scholarly journals Possible Dietary Effects of Insect-Based Diets across Zebrafish (Danio rerio) Generations: A Multidisciplinary Study on the Larval Phase

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 751
Author(s):  
Matteo Zarantoniello ◽  
Basilio Randazzo ◽  
Gloriana Cardinaletti ◽  
Cristina Truzzi ◽  
Giulia Chemello ◽  
...  
Keyword(s):  
Fish Meal ◽  
Gut Health ◽  
Larval Phase ◽  
Nutritional Programming ◽  
Hepatic Lipid Accumulation ◽  
Zebrafish Larvae ◽  
Multidisciplinary Study ◽  
Substitution Level ◽  
Parental Feeding ◽  
Negative Side Effects

Insects represent a valuable and sustainable alternative ingredient for aquafeed formulation. However, insect-based diets have often highlighted controversial results in different fish species, especially when high inclusion levels were used. Several studies have demonstrated that nutritional programming through parental feeding may allow the production of fish better adapted to use sub-optimal aquafeed ingredients. To date, this approach has never been explored on insect-based diets. In the present study, five experimental diets characterized by increasing fish meal substitution levels with full-fat Black Soldier Fly (Hermetia illucens; BSF) prepupae meal (0%, 25%, 50%, 75% and 100%) were used to investigate the effects of programming via broodstock nutrition on F1 zebrafish larvae development. The responses of offspring were assayed through biometric, gas chromatographic, histological, and molecular analyses. The results evidenced that the same BSF-based diets provided to adults were able to affect F1 zebrafish larvae fatty acid composition without impairing growth performances, hepatic lipid accumulation and gut health. Groups challenged with higher BSF inclusion with respect to fish meal (50%, 75% and 100%) showed a significant downregulation of stress response markers and a positive modulation of inflammatory cytokines gene expression. The present study evidences that nutritional programming through parental feeding may make it possible to extend the fish meal substitution level with BSF prepupae meal in the diet up to almost 100% without incurring the well-known negative side effects of BSF-based diets.

scholarly journals Caffeine reduces hepatic lipid accumulation through regulation of lipogenesis and ER stress in zebrafish larvae

2015 ◽  
Vol 22 (1) ◽  
Author(s):  
Xinchun Zheng ◽  
Wencong Dai ◽  
Xiaohui Chen ◽  
Kunyuan Wang ◽  
Wenqing Zhang ◽  
...  
Keyword(s):  
Er Stress ◽  
Lipid Accumulation ◽  
Hepatic Lipid ◽  
Hepatic Lipid Accumulation ◽  
Zebrafish Larvae

scholarly journals Impact of varied combinatorial mixture of non-fishmeal ingredients on growth, metabolism, immunity and gut microbiota of Lates calcarifer (Bloch, 1790) fry

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sanjay K. Gupta ◽  
Ravi Fotedar ◽  
Md. Javed Foysal ◽  
Manisha Priyam ◽  
Muhammad A. B. Siddik ◽  
...  
Keyword(s):  
Growth Performance ◽  
Fish Meal ◽  
Significant Shift ◽  
Gut Health ◽  
Lates Calcarifer ◽  
Poor Growth ◽  
Final Body Weight ◽  
Microbial Profile ◽  
Abundance Profile ◽  
Growth Metabolism

Abstract The search for suitable fish meal replacements in aqua-diets is a salient agenda in the constant effort of making aquaculture practices more sustainable. In this study, we tested four customised diets composed by systematic inclusion of pre-selected fish meal substitutes, lupin kernel meal, BSF meal, TH and PBM on growth, metabolism, cytokine profile, gut morphology and microbiota of juvenile Lates calcarifer. Five isoproteic and isoenergetic diets were prepared viz. FM100 as a control (without fish meal substitute), while FM75, FM50, FM25 and FM0 indicates replacement of fish meal (FM) at 25%, 50%, 75%, and 100%, respectively by a mixture of four different pre-selected non-fish meal (NFM) ingredients. Fish fed FM100, FM75, FM50, FM25 exhibited consistent growth and haematological response, while the fish fed no fishmeal (FM0) showed significant decline in final body weight (FBW) and specific growth rate (SGR). The poor growth performance was correlated with a decrease in villous width, microvilli height and goblet cells density. A significant shift in abundance profile of Psychrobacter in the gut microbial profile of fish fed FM50 was noticed compared to fish fed FM100. The results of qRT-PCR showed up-regulated expression of innate immune responsive genes in the FM50 group. The adverse impacts on growth performance and gut health of fish fed FM0 suggest that the complete substitution of fishmeal is not advisable and the inclusion range of these alternatives should be decided for a species only after examining their effect on maximal physiological performance.

scholarly journals Evaluasi penggunaan tepung cangkang rajungan sebagai bahan baku pakan juwana udang windu Penaeus monodon

2016 ◽  
Vol 15 (2) ◽  
pp. 117
Author(s):  
Agus Kurnia ◽  
Wellem H Muskita ◽  
Oce Astuti ◽  
, Asnani ◽  
Wulandari Harahap
Keyword(s):  
Survival Rate ◽  
Fish Meal ◽  
Penaeus Monodon ◽  
Feeding Trial ◽  
Growth And Survival ◽  
Commercial Diet ◽  
Black Tiger Shrimp ◽  
Substitution Level ◽  
Tiger Shrimp ◽  
C 50

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Feeding trial was conducted to determine the optimum dietary level of the crab shell meal (CSM) for replacement of fish meal (FM) for growth and survival rate of black tiger shrimp larvae. The shrimp (initial weight: 0,0134±0,02 g) were fed with six experimental diet for six weeks which were formulated to replace FM protein by with CSM at various substitution levels: diet A (0% CSM substitution level), diet B (25% CSM substitution level), diet C (50% CSM substitution level), diet D (75% CSM substitution level), diet E (100% CSM substitution level), diet F (commercial diet). Results from the feeding trial indicates that the shrimp fed with all treatment diet were not significantly different in weight gain and FCR. However, survival rate on the shrimp fed with diet F was significantly different to the other groups. The present study conclude that CSM could be used as protein ingredient in the diet of monodon shrimp juvenile.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: replacement, crabs shell meal, fish meal, black tiger shrimp, survival rate</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian pakan dilakukan untuk menentukan dosis optimum tepung cangkang rajungan (TCR) untuk mengganti tepung ikan (TI) dalam pakan terhadap pertumbuhan dan kelangsungan hidup larva udang windu. Udang windu (berat awal: 0,00134±0,02 g ) diberi pakan uji selama enam minggu yang diformulasi untuk mengganti TI dengan tingkat persentasi penggantian TCR dengan desain formulasi pakan A (0% substitusi TCR), pakan B (25% substitusi TCR), pakan C (50% substitusi TCR), pakan D (75% substitusi TCR), pakan E (100% substitusi TCR), dan pakan F (pakan komersial) sebagai pakan kontrol. Hasil dari penelitian ini menunjukkan bahwa udang yang diberi pakan untuk semua perlakuan tidak memberikan pengaruh nyata terhadap pertumbuhan dan laju pertumbuhan harian. Akan tetapi kelompok udang yang diberi pakan F berpengaruh nyata terhadap kelangsungan hidup dibanding dengan perlakuan lainnya. Hasil penelitian ini menyimpulkan bahwa tepung cangkang rajungan dapat digunakan sebagai bahan pakan sumber protein dalam pakan juvenile udang windu.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: penggantian bahan, tepung cangkang rajungan, tepung ikan, udang windu</p>

scholarly journals Response of exotic turkey (Meleagris gallopavo) poults to diets substituted with varying levels of hydrolysed feather meal or blood meal as partial replacement for fish meal

2020 ◽  
Vol 47 (2) ◽  
pp. 100-106
Author(s):  
A. A. Adegbenjo ◽  
O. O. Oluwatosin ◽  
O. A. Osinowo ◽  
A. O. Oso ◽  
O. M. Sogunle ◽  
...  
Keyword(s):  
Blood Meal ◽  
Fish Meal ◽  
Meleagris Gallopavo ◽  
Nutrient Digestibility ◽  
Partial Replacement ◽  
Feather Meal ◽  
Protein Sources ◽  
Substitution Level ◽  
Turkey Poults ◽  
Meal Protein

Recent concerns over the seasonal availability and high cost of conventional protein sources has necessitated the search for alternative feed protein with lesser feed-food competitive properties yet desirable and appropriate to poults. A study was designed to determine the response of exotic turkey poults (Meleagris gallopavo) to diets substituted with varying levels of hydrolysed feather meal (HFM) or blood meal (BLM) as partial replacement for fishmeal for 112days. One hundred and ninety-two British United Turkeys BUT) were allotted to eight dietary treatments comprising four replicates and six birds per replicate. The birds were arranged in a 2 × 4 factorial arrangement of two protein sources (Hydrolysed feather meal or Blood meal) included at 4 levels (0, 10, 20 and 30%). Diets 1-4 were formulated such that fish meal in control was replaced by feather meal, protein for protein. Diets 5-8 wereformulated such that fish meal in control was replaced by blood meal, protein for protein and included at 0, 10, 20 and 30%. Treatments 1 and 5 were the control groups. Data obtained were subjected to Analysis of Variance using SAS. Turkeys fed test ingredients at 20% substitution level recorded higher (p<0.05) final weight and weight gain and best FCR at starter phase. Cost of feed/kg diet reduced (p<0.05) as the substitution level of test ingredientincreased. Nutrient digestibility and energy metabolisability increased with increased substitution levels of test ingredients at the starter phase. Starter turkeys fed hydrolysed feather meal recorded higher energy metabolisability. It can be concluded from this study that substituting turkey diets with Hydrolysed feather meal and Blood meal at 20% inclusion level improves performance, nutrient digestibility and energy metabolisability.

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