scholarly journals Effect of Fermented Rapeseed Meal on the Gastrointestinal Microbiota and Immune Status of Rabbit (Oryctolagus cuniculus)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 716
Author(s):  
Łukasz Wlazło ◽  
Dorota Kowalska ◽  
Paweł Bielański ◽  
Anna Chmielowiec-Korzeniowska ◽  
Mateusz Ossowski ◽  
...  
Keyword(s):  
Immune Status ◽  
System Development ◽  
Anaerobic Bacteria ◽  
Rapeseed Meal ◽  
Control Group ◽  
Standard Diet ◽  
Immunoglobulin Level ◽  
Gastrointestinal Microbiota ◽  
Bioactive Substances ◽  
Immune System Development

The present study was conducted to determine the effect of the use of varying amounts of fermented rapeseed meal in diets for rabbits on the immune status and microbiota of segments of the GIT. Forty 35 day old rabbits used in the experiment were assigned to four groups: the control group (group C) were fed a standard diet and the experimental received 4%, 8% or 12% fermented rapeseed meal (included in place of standard soybean meal). Class A, G and M immunoglobulins were determined in the blood plasma. In the food content collected after slaughter, microbiological parameters were determined for individual sections of the digestive tract. Rabbits from the groups receiving a diet with an increased proportion of fermented rapeseed meal (8% or 12%) had lower concentrations of anaerobic bacteria and Escherichia coli in the intestinal contents. Research has shown that the increase in intake of fermented rapeseed meal was correlated with an increase in the correlations between the immunoglobulin level and the size of the microbial population in the GIT. In light of the presented results fermented rapeseed meal, by supplying valuable bioactive substances, appears to be a good component in the diet of rabbits, enhancing immune system development and helping to prevent disturbances of the gut microbiota.

scholarly journals Impact of synbiotic diets including inulin, Bacillus coagulans and Lactobacillus plantarum on intestinal microbiota of rat exposed to cadmium and mercury

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Dornoush Jafarpour ◽  
Seyed Shahram Shekarforoush ◽  
Hamid Reza Ghaisari ◽  
Saeid Nazifi ◽  
Javad Sajedianfard
Keyword(s):  
Heavy Metals ◽  
Intestinal Microbiota ◽  
Anaerobic Bacteria ◽  
Bacillus Coagulans ◽  
Treated Group ◽  
Inhibitory Effect ◽  
Control Group ◽  
Standard Diet ◽  
Male Wistar Rats ◽  
Group 1

The aim of this study was to investigate the efficacy of two probiotics and a prebiotic (inulin) on intestinal microbiota of rats exposed to cadmium and mercury. Fifty-four male Wistar rats were randomly divided into nine groups. All groups except control group were fed standard rat chow with 5% inulin and treated as follows: i) control (standard diet), ii) <em>Lactobacillus</em> <em>plantarum</em>- treated group (1×109 CFU/day), iii) <em>Bacillus</em> <em>coagulans</em>-treated group (1×109 spores/day), iv) cadmium-treated group (200 μg/rat/day), v) L. plantarum and cadmium-treated group, vi) <em>B. coagulans</em> and cadmium-treated group, vii) mercury-treated group (10 μg/rat/day), viii) <em>L. plantarum</em> and mercurytreated group, ix) <em>B. coagulans</em> and mercurytreated group. Cadmium, mercury and probiotics were daily gavaged to individual rats for 42 days. Treatment effects on intestinal microbiota composition of rats were determined. Data showed that cadmium and mercury accumulation in rat intestine affected the gastrointestinal tract and had a reduction effect on all microbial counts (total aerobic bacteria, total anaerobic bacteria, total Lactic acid bacteria, <em>L. plantarum</em> and <em>B. coagulans</em> counts) compared to the control group. It was also observed that application of synbiotics in synbiotic and heavy metals-treated groups had a significant effect and increased the number of fecal bacteria compared to the heavy metals groups. Based on our study, it can be concluded that <em>L. plantarum</em> and <em>B. coagulans</em> along with prebiotic inulin play a role in protection against cadmium and mercury inhibitory effect and have the potential to be a beneficial supplement in rats’ diets.

Thyroid function, metabolic indices and growth performance in pigs fed 00-rapeseed meal

2003 ◽  
Vol 51 (3) ◽  
pp. 283-295 ◽  
Author(s):  
A. Svetina ◽  
I. Jerković ◽  
Ljiljana Vrabac ◽  
S. Ćurić
Keyword(s):  
Thyroid Gland ◽  
Growth Performance ◽  
Thyroid Function ◽  
Rapeseed Meal ◽  
Daily Weight Gain ◽  
Control Group ◽  
Standard Diet ◽  
Metabolic Indices ◽  
Experimental Group ◽  
Fattening Pigs

Thyroid function and morphology, liver morphology, some metabolic indices, haematological parameters and growth performance of fattening pigs fed 00-rapeseed meal (00-RPM) were examined. The control group was fed on a standard diet containing 6% sunflower seed meal (SM) during the growing period and 8% during the finishing period. The first experimental group was fed a diet in which SM was replaced by equal (6% and 8%) amounts of 00-RPM. The second experimental group was fed with a higher (8% and 10%) amount of 00-RPM. There were no significant differences between the control and experimental groups in the serum concentrations of triiodothyronine (T3) and thyroxine (T4). Thyroid gland and liver weights were significantly (P < 0.01) higher in both groups fed 00-RPM than in the group fed SM. The epithelium of the thyroid gland was cuboidal or columnar and the follicular area was moderately enlarged in pigs fed 00-RPM. Marked changes in liver histology were not observed. The 00-RPM diet increased (P < 0.01) the serum values of total proteins in the first fattening period. At the end of fattening both groups fed 00-RPM had higher (P < 0.05; P < 0.01) concentrations of plasma glucose than the control group. The inclusion of 10% of 00-RPM during the finishing period increased (P < 0.05) the serum values of insulin. Daily weight gain during the growing and the finishing period was higher (P < 0.05) in the experimental groups than in the control group. The results suggest that 6-10% 00-RPM can be used as a protein source in the diet of fattening pigs without poisonous side effects.

scholarly journals ​Response of In ovo Supplementation of Amino Acid and Minerals on Egg Hatchability of Broiler Chicken

2021 ◽  
Author(s):  
M. Saravanakumar ◽  
A.V. Elangovan ◽  
V.B. Awachat ◽  
A.K. Pattanaik ◽  
A. Dhali ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chicken ◽  
System Development ◽  
Control Group ◽  
In Ovo ◽  
Egg Hatchability ◽  
Group I ◽  
Immune System Development ◽  
Group Ii

Background: In ovo supplementation of nutrients either amino acids or minerals in broiler chicken has been common more often individually or few nutrients with positive results. In view of the above, in ovo supplementation with combination of amino acids and minerals as a complete nutrient capsule for the gut and immune system development was tested for hatchability since the modern commercial broilers are very fragile to any changes in the internal environment. Methods: Four trials were conducted to study the response of in ovo supplementation of combinations of Lys, Met, Arg, Thr, Glu, Zn, Se and Cu on hatchability of broiler eggs. In trial I, the fertile eggs on day 18 were divided into five groups of 32 eggs in each group. Group I served as control without any amino acids and mineral supplementation. Group II, III, IV and V supplemented with Lys, Met, Arg, Thr, Glu, Zn, Se and Cu @ 2.2, 1, 2.5, 1.6, 2.5 mg, 80, 0.3, 16 µg; 4.4, 2, 5, 3.2, 5 mg, 80, 0.3, 16 µg; 11, 5, 12.5, 8, 12.5 mg, 80, 0.3, 16 µg and 22, 10, 25, 16, 25 mg, 80, 0.3, 18 µg respectively. In trial II, seventy fertile eggs were divided into seven groups, Group I served as control. Group II, III, IV, V, VI and VII were supplemented with graded levels (1x to 6x) of amino acids. The 1x consisted of Lys, Met, Arg, Thr and Glu @ 2.2, 1, 2.5, 1.6 and 2.5 mg, respectively. On day 18, 10 eggs per group were supplemented with graded levels of AA combination into the amniotic fluid. In trail III and IV it was similar to trial II but was without Lys and Met, respectively. Result: Results indicated that hatchability (Trial 1) in Group V, IV, III, II and I were 0, 18, 21.8, 71 and 90.6%, respectively. The Combination of five amino acids (Trial II) (Lys, Met, Thr, Arg, Glu) for in ovo supplementation reduced the hatchability, whereas, combination of four amino acids (Trial III) (Met, Thr, Arg, Glu) without Lys at lower doses resulted in good hatchability (86.6%). In ovo supplementation of amino acid or mineral-amino acid combinations in commercial broiler eggs should be restricted to few amino acids.

scholarly journals The effects of a fermented rapeseed meal or/and soybean meal additive on the blood lipid profile and immune parameters of piglets and on minerals in their blood and bone

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253744
Author(s):  
Anna Czech ◽  
Eugeniusz R. Grela ◽  
Bożena Nowakowicz-Dębek ◽  
Łukasz Wlazło
Keyword(s):  
Soybean Meal ◽  
Blood Lipid ◽  
Rapeseed Meal ◽  
Control Group ◽  
Standard Diet ◽  
Blood Lipid Profile ◽  
Fermented Soybean ◽  
Immune Parameters ◽  
Metacarpal Bones ◽  
Lipid Parameters

The aim of the study was to assess the effect of the inclusion of dried fermented soybean and/or rapeseed meal in piglet feed on immune parameters, blood lipid parameters, and mineral content in the blood and metacarpal bones. The study was conducted on 150 28-day-old piglets divided into 5 groups. Piglets in the control group (C) received a standard diet with soybean meal. Animals in group FR (group receiving a diet with 8% FRSM) received a diet in which a portion of the soybean meal was replaced with 8% dried fermented rapeseed meal (FRSM). Animals in group FR/FS received a diet in which a portion of the soybean meal was replaced with 6% FRSM and 2% fermented dried soybean meal (FSBM). The piglets in group FS/FR received a diet with 6% FSBM and 2% FRSM. Group FS received a diet in which a portion of the soybean meal was replaced with an 8% share of FSBM. The inclusion of 8% or 6% fermented rapeseed meal (group FR or FR/FS) in the diet of piglets had a beneficial effect on their immune status, as evidenced by the increase in plasma levels of IgG and IgA and the decrease in IL-6 relative to the control group. It also significantly increased the concentrations of minerals, i.e. P, Ca and Zn, in the blood plasma and metacarpal bones of piglets and improved the availability of iron, a key bioelement involved in haemoglobin. The use of 8% or 6% fermented soybean meal in the diet (groups FS and FS/FR) of piglets had a positive effect on blood lipid parameters, reducing CHOL and LDL-cholesterol in the plasma. In conclusion, the fermentation process enables better utilization of rapeseed or soybean meal by pigs. Dried fermented rapeseed meal could partially replace protein components from GMO (genetically modified ogranism) crops (soybean meal) used in diets for pigs.

scholarly journals Milk Replacer Supplementation with Docosahexaenoic Acid from Microalgae Does Not Affect Growth and Immune Status in Goat Kids

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1233
Author(s):  
Isabel Moreno-Indias ◽  
Lorenzo E. Hernández-Castellano ◽  
Davinia Sánchez-Macías ◽  
Antonio Morales-delaNuez ◽  
Alexandr Torres ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Immune Status ◽  
System Development ◽  
Milk Replacer ◽  
Fatty Acid Profiles ◽  
Negative Effects ◽  
Beneficial Effects ◽  
Immune System Development ◽  
Nutritional Strategies ◽  
The Complement System

Consumption of polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (DHA), has beneficial effects for consumers’ health. Consequently, there is an increased interest in enhancing meat fatty acid profiles (i.e., PUFA and DHA content) through diverse nutritional strategies. This study aimed to investigate the effect of supplementing a microalgae-derived product rich in DHA on growth and immune system development in newborn goat kids. In this experiment, newborn goat kids were fed milk replacer (MR) supplemented with three levels of a microalgae-derived product rich in DHA (DHA-Gold®, Martek Biosciences, MD, USA). Groups were designed as follows: MR-NS (milk replacer without DHA-Gold® supplementation; n = 10), MR-DHA-9 (9 g of DHA-Gold®/L milk replacer; n = 10) and MR-DHA-18 (18 g of DHA-Gold®/L milk replacer; n = 10). The immune status of the kids was evaluated by the plasma IgG and IgM concentrations, as well as by the complement system and chitotriosidase activities. Dietary supplementation with DHA did not affect either growth or innate and humoral immunity (p > 0.05). This study concludes that supplementation with DHA does not cause negative effects on growth and immune status in newborn goat kids.

scholarly journals Influence of technological processing on lipid-lowering activity of substances containing in porcine hearts and aortas

10.5219/1119 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 331-336
Author(s):  
Elena Kotenkova ◽  
Irina Chernukha
Keyword(s):  
Molecular Weight ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Raw Material ◽  
Serum Lipid Profile ◽  
Lipid Lowering ◽  
Control Group ◽  
Standard Diet ◽  
Bioactive Substances ◽  
Wide Range

Edible by-products are a good source of nutrients and bioactive substances and could be used as functional ingredients or for biopeptides production natively contained in raw materials. A wide range of peptides are also formed during the enzymatic hydrolysis or food processing. The comparative results of the effectiveness of isolated certain protein and peptide fractions by ultrafiltration with the same natively presented in raw tissues, as well as the influence of heat treatment on biological activity of origin active substances are presented. The model of rat alimentary hyperlipidemia was developed by adding cholesterol and fat to the standard diet and vitamin D2 injection per os. Serum lipid profile was determined on automatic analyzer BioChem FC-360. Dynamic of changes in serum lipid profile was assessed as corresponding control group medium results in ratio to certain rat data. Two-dimensional electrophoresis (2DE) was performed according to the method of O’Farrell with isoelectric focusing in ampholine pH gradient (IEF-PAGE) with following identification by MALDI-TOF MS and MS/MS mass spectrometry. Consumption of native pig aorta and pig heart during 14th days led to normalization of lipid profile in serum of hyperlipidemic rats, while low molecular weight (LMUF, MW <5 kDa) and medium molecular weight (MMUF, MW = 5 – 30 kDa) ultrafiltrates of pig aorta extract did not strongly influenced on level of triglicerides and, on contrary, elevated high density cholesterol. Consumption of developed product by hyperlipidemic rats during 28th days did not lead to significant changes in serum lipid profile, while on 42nd day all ratios reached ones in group, which were treated with native raw material or isolated active fractions. The stability of developed product was confirmed by proteomic studies. Obtained results open prospects to modernization the technology, presumably use as a matrix dietary meat (e.g. poultry) with incorporated active identified components.

scholarly journals Effect of fermented rapeseed meal as a feed component on the redox and immune system of pregnant sows and their offspring

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Anna Czech ◽  
Anna Stępniowska ◽  
Martyna Kiesz
Keyword(s):  
Immune System ◽  
Vitamin C ◽  
Blood Plasma ◽  
Rapeseed Meal ◽  
Control Group ◽  
Standard Diet ◽  
Lipid Hydroperoxides ◽  
Lactating Sows ◽  
Mda Content ◽  
Wbc Count

Abstract The aim of the study was to assess the effect of dried fermented rapeseed meal (FRSM) in diets for sows on blood redox and immunological parameters, taking into account the physiological period (pregnancy or lactation) and age (primiparous vs multiparous sows). The experiment also aimed to determine how FRSM administered to pregnant sows, affect the antioxidant and immune systems of piglets. The animals were divided to 4 groups of 15 animals each. Control groups of primiparous (CG) and multiparous sows (CS) received a standard diet for pregnant or lactating sows. Experimental groups of primiparous (EG) and multiparous sows (ES) received feed with a 4% share of FRSM in place of soybean meal up to 100 day of gestation, 9% share of FRSM from 100 day of gestation to 7 day of lactation, and then again 4% share of FRSM until the end of lactation. In the blood plasma of pregnant sows fed diet with FRSM addition, higher FRAP value and vitamin C, uric acid (UA), immunoglobulin IgG content, lymphocytes (LYM) count and a lower content of malondialdehyde (MDA), lipid hydroperoxides (LOOH), immunoglobulin IgM was noted than in the blood plasma of control sows. Both primiparous and multiparous lactation sows whose feed included FRSM had higher catalase (CAT) activity, higher FRAP, vitamin C, immunoglobulin IgG and IL-6 content, and lower UA content than the control sows. Piglets born to sows fed diet with FRSM addition had significantly higher FRAP values, vitamin C, IgG, and IL-6 content and white blood cells (WBC) count and lower MDA and UA content in the blood plasma than piglets born to sows from control group. Multiparous sows compared to primiparous sows had higher CAT activity, and higher vitamin C, LOOH, creatinine (CREAT), and IgM content. Elevated FRAP, and CREAT levels and reduced MDA content were also observed in the plasma of the multiparous sows compared to primiparous sows during lactation. Multiparous lactation sows compared to primiparous sows had lower WBC count, and IgG and IgM content. Piglets born to multiparous sows had higher FRAP values, LOOH content and IgA content while lower MDA content compared to piglets born to primiparous sows. The inclusion of dried fermented rapeseed meal in feed for sows significantly stimulates antioxidant processes in primiparous and multiparous sows and in their piglets. The inclusion of dried fermented rapeseed meal in the diet of sows stimulates antioxidant processes in primiparous and multiparous sows and in their piglets. This is responsible for stimulation of the immune system (increased LYM counts and IgG titres in the blood plasma). The improved antioxidant status in the plasma suggests that dried fermented rapeseed meal stimulated the immune system of pregnant and lactating sows and their new-born off spring.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

PERAN AIR REBUSAN DAUN SALAM (SYZGIUM POLYANTHUM) DALAM MENURUNKAN KADAR ASAM URAT

2016 ◽  
Vol 5 (2) ◽  
pp. 83-91
Author(s):  
Miftafu Darussalam ◽  
Dwi Kartika Rukmi
Keyword(s):  
Uric Acid ◽  
Vascular Inflammation ◽  
Vascular Lesion ◽  
Wilcoxon Test ◽  
Test Time ◽  
Control Group ◽  
Target Area ◽  
Bioactive Substances ◽  
Control Group Design ◽  
Boiled Water

Background: Uric acid is a final product or a waste that is resulted from the metabolism of purines. A high level of uric acid (hyperuricemia) will cause several health problems, such as vascular inflammation, smooth muscle proliferation, and vascular lesion in kidneys. The syzygium polyanthum leaves contain bioactive substances that may affect the level of uric acid in blood. Objective: This study aimed to determine the influence of boiled water of syzygium polyanthum leaves to the changes of uric acid levels in the target area of Puskesmas Pandak 1 Bantul. Methods: This study employed pre- and post-test without control group design. The population consisted of all patients with hyperuricemia in the target area of Puskesmas Pandak 1 Bantul. Sample was selected with a concecutive sampling, gaining a total number of 24 respondents. Data were analyzed with the Wilcoxon test. The dose of boiled water of syzygium polyanthum leaves intake was 0.36g/ KgBW, once a day for 14 days. Result: This research showed that the boiled water of syzygium polyanthum leaves decreased hyperuricemia (uric acid levels), along with the significancy value of 0.009 (p <0.05). At the pre-test time, the average level of uric acid reached 7.279 mg/dl, and after the treatment, it decreased to 6.76 mg/dl. Conclusion: This study has established evidence that the boiled water of syzygium polyanthum leaves is able to decrease hyperuricemia (uric acid level in blood). Keywords: syzygium polyanthum, boiled water of syzygium polyanthum leaves, hyperuricemia

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

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