scholarly journals Comparative Transcriptome Profile between Iberian Pig Varieties Provides New Insights into Their Distinct Fat Deposition and Fatty Acids Content

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 627
Author(s):  
Ana Villaplana-Velasco ◽  
Jose Luis Noguera ◽  
Ramona Natacha Pena ◽  
Maria Ballester ◽  
Lourdes Muñoz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
Rna Seq ◽  
Transcriptome Profile ◽  
Causal Genes ◽  
Iberian Pig ◽  
Two Parameters ◽  
Upregulated Genes ◽  
Imf Content

The high deposition of intramuscular fat and the content of oleic fatty acid are characteristic of the Iberian pig. These two parameters present great variability and are differentiated amongst the varieties that make up the Iberian pig population. Although previous studies generated evidence for causal genes and polymorphisms associated to the adipogenic potential of the Iberian pig, there is little information about how genetic expression influences this trait’s variability. The aim of this study was to analyses the expression profile between two varieties of Iberian pig (Torbiscal and Retinto) and their reciprocal crosses differentiated in their intramuscular fat (IMF) content and fatty acid (FA) composition in the Longissimus thoracis muscle using an RNA-seq approach. Our results corroborate that the Retinto variety is the fattiest amongst all studied varieties as its upregulated genes, such as FABP3 and FABP5, SLC27A1 and VEGFA among others, contribute to increasing adiposity. In its turn, Torbiscal pigs showed an upregulation of genes associated with the inhibition of fat deposition such as ADIPOQ and CPT1A. Further genetic variation analysis in these Iberian varieties showed relevant associations for SNP located within the differentially expressed genes with IMF and FA content. Thus, the differences found in the genetic architecture and the muscle transcriptome of these Iberian varieties might explain the variability in their fat content and composition and hence, their meat quality.

scholarly journals Expression of lipid metabolism genes provides new insights into intramuscular fat deposition in Laiwu pigs

2020 ◽  
Vol 33 (3) ◽  
pp. 390-397 ◽  
Author(s):  
Hui Wang ◽  
Jin Wang ◽  
Dan-dan Yang ◽  
Zong-li Liu ◽  
Yong-qing Zeng ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Molecular Mechanisms ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
Fatty Acid Binding ◽  
Fat Removal ◽  
Lipid Metabolism Genes ◽  
Imf Content

Objective: The objective of this study was to measure the special expression pattern of lipid metabolism genes and investigate the molecular mechanisms underlying intramuscular fat (IMF) deposition in Longissimus dorsi muscle of Laiwu pigs.Methods: Thirty-six pigs (Laiwu n = 18; Duroc×Landrace×Yorkshire n = 18) were used for the measurement of the backfat thickness, marbling score, IMF content, and expression of lipid metabolism genes.Results: Significant correlations were found between IMF content and the mRNA expression of lipid metabolism genes. Of the 14 fat deposition genes measured, fatty acid synthase (FASN) showed the strongest correlation (r = 0.75, p = 0.001) with IMF content, and of the 6 fat removal genes, carnitine palmitoyl transferase 1B (CPT1B) exhibited the greatest negative correlation (r = –0.66, p = 0.003) with IMF content in Laiwu pig. Multiple regression analysis showed that CPT1B, FASN, solute carrier family 27 member 1 (SLC27A1), and fatty acid binding protein 3 (FABP3) contributed 38% of the prediction value for IMF content in Laiwu pigs. Of these four variables, CPT1B had the greatest contribution to IMF content (14%) followed by FASN (11%), SLC27A1 (9%), and FABP3 (4%).Conclusion: Our results indicate that the combined effects of an upregulation in fat deposition genes and downregulation in fat removal genes promotes IMF deposition in Laiwu pigs.

scholarly journals Association of osteopontin gene with intramuscular fat content and fatty acid composition traits in pigs

2020 ◽  
Vol 19 (2) ◽  
pp. 197-208
Author(s):  
Nanthana Pothakam ◽  
◽  
Worrarak Norseeda ◽  
Guisheng Liu ◽  
Tawatchai Teltathum ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
Fa Composition ◽  
Muscle Tissues ◽  
Ether Extraction ◽  
Pcr Rflp ◽  
Imf Content

Osteopontin (OPN) is a secreted phosphoprotein that is involved in the development of skeletal muscle and fat deposition. The objectives of this study were to identify the polymorphism of the OPN gene and to analyze the association of the OPN gene with intramuscular fat (IMF) content and fatty acid (FA) composition in pigs. Longissimus thoracis (LT) muscle samples taken from the 10-11th rib were collected from a total of 328 Duroc pigs. Genomic DNA samples were extracted from LT muscle tissues using the phenol-chloroform method. IMF content was measured using the ether extraction method and FA composition was measured by gas chromatography. The porcine OPN polymorphisms were identified by DNA sequencing and were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The association analysis of the OPN gene with IMF and FA composition traits was performed using a general linear model (GLM). Two polymorphic sites (OPN g.2442-2471indel and g.3836A>G) were found in the 5´-flanking region and intron 1 of the porcine OPN gene. The OPN g.2442-2471indel polymorphism was found to be significantly associated with IMF content and ω3 FA levels (P<0.05). Moreover, OPN g.3836A>G polymorphism was significantly associated with the linolenic acid levels in the muscles of pigs (P<0.05). The results of this study indicate that the OPN gene is important to IMF content, as well as linolenic and ω3 FA levels in pigs, and could be used as a candidate gene to improve fat deposition and fatty acid composition in the muscles of pigs.

scholarly journals RNA-Seq Analysis Reveals Hub Genes Involved in Chicken Intramuscular Fat and Abdominal Fat Deposition During Development

2020 ◽  
Vol 11 ◽  
Author(s):  
Siyuan Xing ◽  
Ranran Liu ◽  
Guiping Zhao ◽  
Lu Liu ◽  
Martien A. M. Groenen ◽  
...  
Keyword(s):  
Intramuscular Fat ◽  
Abdominal Fat ◽  
Fat Deposition ◽  
Rna Seq ◽  
Hub Genes ◽  
Abdominal Fat Deposition

scholarly journals Effects of dietary lysine level on the content and fatty acid composition of intramuscular fat in late-stage finishing pigs

2018 ◽  
Vol 98 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Taiji Wang ◽  
Mark A. Crenshaw ◽  
Naresh Regmi ◽  
Brian J. Rude ◽  
M. Shamimul Hasan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Late Stage ◽  
National Research Council ◽  
Intramuscular Fat ◽  
Finishing Pigs ◽  
Treatment Groups ◽  
Fa Composition ◽  
Ad Libitum ◽  
Imf Content ◽  
The Imf

This study was conducted to investigate how dietary lysine level affects the intramuscular fat (IMF) content and fatty acid (FA) composition in late-stage finishing pigs. Nine crossbred barrows [94.4 ± 6.7 kg body weight (BW)] were randomly allotted to three treatment groups (n = 3). Three corn- and soybean-meal-based diets were formulated to meet the National Research Council (2012) requirements for various nutrients except for lysine, whose concentrations were 0.43%, 0.71%, and 0.98% (as-fed basis) for Diets 1 (lysine-deficient), 2 (lysine-adequate), and 3 (lysine-excess), respectively. After 5 wk of ad libitum access to diets, pigs were harvested and longissimus dorsi samples were collected. The IMF content and FA composition of the samples were analyzed by gas chromatography. Results showed that the IMF content of the muscle was increased linearly (P < 0.05) with decreasing dietary lysine level from 0.98% to 0.43%. Dietary lysine level altered the composition of FA, especially the unsaturated FA, in the muscle. Particularly, the percentages of C18:1 n-9 and total monounsaturated FA were higher, whereas the percentages of C18:2 n-6 and total polyunsaturated FA were lower, in the muscle of the pigs fed Diet 1. Collectively, dietary lysine deficiency increased the proportion of monounsaturated FA and decreased the proportion of polyunsaturated FA, which may benefit pork palatability.

Effect of a high dose of CLA in finishing pig diets on fat deposition and fatty acid composition in intramuscular fat and other fat depots

Meat Science ◽  
2013 ◽  
Vol 93 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Núria Tous ◽  
Rosil Lizardo ◽  
Borja Vilà ◽  
Marina Gispert ◽  
Maria Font-i-Furnols ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
High Dose ◽  
Fat Depots ◽  
Finishing Pig

Genetic parameters and crossbreeding effects of fat deposition and fatty acid profiles in Iberian pig lines1

2016 ◽  
Vol 94 (1) ◽  
pp. 28-37 ◽  
Author(s):  
N. Ibáñez-Escriche ◽  
E. Magallón ◽  
E. Gonzalez ◽  
J. F. Tejeda ◽  
J. L. Noguera
Keyword(s):  
Fatty Acid ◽  
Genetic Parameters ◽  
Fat Deposition ◽  
Fatty Acid Profiles ◽  
Iberian Pig

scholarly journals Association of IFNA16 and TNFRSF19 Polymorphisms with Intramuscular Fat Content and Fatty Acid Composition in Pigs

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 109
Author(s):  
Supamit Mekchay ◽  
Nanthana Pothakam ◽  
Worrarak Norseeda ◽  
Pantaporn Supakankul ◽  
Tawatchai Teltathum ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Arachidic Acid ◽  
Tumor Necrosis Factor Receptor ◽  
Intramuscular Fat ◽  
Potential Candidate ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Fa Composition ◽  
Crossbred Pigs ◽  
Imf Content

Interferon-alpha-16 (IFNA16) and tumor necrosis factor receptor superfamily member 19 (TNFRSF19) are cytokines that may play a role in adipogenesis and fatness. Single nucleotide polymorphisms (SNPs) of the porcine IFNA16 and TNFRSF19 genes were verified and their association with intramuscular fat (IMF) content and fatty acid (FA) composition were evaluated in commercial crossbred pigs. Two non-synonymous SNPs of the porcine IFNA16 c.413G > A and TNFRSF19 c.860G > C loci were detected in commercial crossbred pigs. The porcine IFNA16 c.413G >A polymorphism was significantly associated with stearic acid, total saturated FAs (SFAs), and the ratio of monounsaturated FAs (MUFAs) to SFAs (p < 0.05). Furthermore, the porcine TNFRSF19 c.860G > C polymorphism was found to be significantly associated with IMF content and arachidic acid levels (p < 0.05). The results revealed that porcine IFNA16 and TNFRSF19 polymorphisms are related to IMF content and/or FA composition and affirmed the importance of these cytokine genes as potential candidate genes for lipid deposition and FA composition in the muscle tissue of pigs.

scholarly journals Identification of Candidate Genes That Specifically Regulate Subcutaneous and Intramuscular Fat Deposition Using Transcriptomic and Proteomic Profiles in Dingyuan Pigs

2021 ◽  
Author(s):  
Pan Zhang ◽  
Qinggang Li ◽  
Yijing Wu ◽  
Yawen Zhang ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Subcutaneous Fat ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
Meat Production ◽  
Rna Seq ◽  
Phenotypic Differences ◽  
Fat Thickness ◽  
Key Pathways ◽  
Differentially Abundant Proteins

Abstract Subcutaneous fat and intramuscular fat (IMF) deposition are closely related to meat production and pork quality. Dingyuan pig, as a native pig breed in China, low selection leads to obvious genetic and phenotypic differences in the population. Individuals with extreme fat content in the population are ideal models for studying the mechanism of fat deposition. In this study, we used RNA-Seq and tandem mass tags-based (TMT) proteomics to analyze the key pathways and genes that specifically regulate subcutaneous fat and IMF deposition in Dingyuan pigs. We identified 191 differentially expressed genes (DEGs) and 61 differentially abundant proteins (DAPs) in the high and low back fat thickness (HBF, LBF) groups, 85 DEGs and 12 DAPs were obtained in the high and low intramuscular fat (HIMF, LIMF) groups. The functional analysis showed that the DEGs and DAPs in the backfat groups were mainly involved in carbohydrates, amino acids, and fatty acids, whereas the IMF groups were involved in the insulin pathway, longevity, and some disease-related pathways. we found 33 candidate genes that might tissue-specifically lipids deposition for subcutaneous and intramuscular fat. Our research provides theoretical reference materials for the improvement of fat deposition traits of local pig breeds in my country.

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