scholarly journals A Seasonal Study of Koi Herpesvirus and Koi Sleepy Disease Outbreaks in the United Kingdom in 2018 Using a Pond-Side Test

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 459
Author(s):  
Irene Cano ◽  
John Worswick ◽  
Brian Mulhearn ◽  
David Stone ◽  
Gareth Wood ◽  
...  
Keyword(s):  
Common Carp ◽  
Internal Control ◽  
Limit Of Detection ◽  
Disease Outbreaks ◽  
Lamp Assay ◽  
Reference Laboratory ◽  
Koi Herpesvirus ◽  
The United Kingdom ◽  
European Genotype ◽  
The Common

Fluorescence real-time LAMP assays were designed for the orf43 gene of CyHV-3 European genotype and the p4a gene of the CEV genogroup I. A third LAMP assay to detect the ef1a gene of the host common carp was designed as an internal control. The limit of detection was 102 and 103 viral copies under 25 min for CyHV-3 and CEV, respectively. The specificity of the CyHV-3 LAMP assay was 95.6% of 72 fish herpesviruses tested. Sixty-three non-lethal common carp mucus swabs were collected across 16 sites during disease investigations. DNA extractions were performed in under 10 min using the QuickExtract™ digestion buffer. The LAMP amplification of CyHV-3 DNA in mucus swabs from clinical cases was detected from 4 to 13 min in 13 sites, while a co-infection of CyHV-3 and CEV was confirmed by LAMP in a single site. The LAMP results agreed with the results of the reference laboratory. The common carp ef1a was amplified only in 61% of the mucus swabs collected, preventing its use as a robust internal control to distinguish false negatives from invalid tests. After further optimization, these tests could be implemented for border inspection posts surveillance and decentralizing testing during disease outbreaks.

scholarly journals Cross-Institute Evaluations of Inhibitor-Resistant PCR Reagents for Direct Testing of Aerosol and Blood Samples Containing Biological Warfare Agent DNA

2013 ◽  
Vol 80 (4) ◽  
pp. 1322-1329 ◽  
Author(s):  
Timothy D. Minogue ◽  
Phillip A. Rachwal ◽  
Adrienne Trombley Hall ◽  
Jeffery W. Koehler ◽  
Simon A. Weller
Keyword(s):  
United States ◽  
United Kingdom ◽  
Internal Control ◽  
Limit Of Detection ◽  
Brucella Melitensis ◽  
The United States ◽  
Content Type ◽  
The United Kingdom ◽  
Direct Testing ◽  
Pcr Assays

ABSTRACTRapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexedBacillus anthracis(target) andBacillus subtilis(internal-control) PCR was used to evaluate 4 reagents against 5 PCR inhibitors and down-selected the TaqMan Fast Virus 1-Step master mix (Life Technologies Inc.). In the United States, four real-time PCR assays (targetingB. anthracis,Brucella melitensis, Venezuelan equine encephalitis virus [VEEV], andOrthopoxvirusspp.) were used to evaluate 5 reagents (plus the Fast Virus master mix) against buffer, blood, and soil samples and down-selected the KAPA Blood Direct master mix (KAPA Biosystems Inc.) with added PlatinumTaq(Life Technologies). The down-selected reagents underwent further testing. In the United Kingdom experiments, both reagents were tested against seven contrived aerosol collector samples containingB. anthracisAmes DNA andB. subtilisspores from a commercial formulation (BioBall). In PCR assays with reaction mixtures containing 40% crude sample, an airfield-collected sample induced inhibition of theB. subtilisPCR with the KAPA reagent and complete failure of both PCRs with the Fast Virus reagent. However, both reagents allowed successful PCR for all other samples—which inhibited PCRs with a non-inhibitor-resistant reagent. In the United States, a cross-assay limit-of-detection (LoD) study in blood was conducted. The KAPA Blood Direct reagent allowed the detection of agent DNA (by four PCRs) at higher concentrations of blood in the reaction mixture (2.5%) than the Fast Virus reagent (0.5%), although LoDs differed between assays and reagent combinations. Across both groups, the KAPA Blood Direct reagent was determined to be the optimal reagent for inhibition relief in PCR.

scholarly journals Koi Herpesvirus Disease (KHVD)

EDIS ◽  
2013 ◽  
Vol 2013 (5) ◽  
Author(s):  
Kathleen H. Hartman ◽  
Roy P. Yanong ◽  
Deborah B. Pouder ◽  
Barbara Denise Petty ◽  
Ruth Francis-Floyd ◽  
...  
Keyword(s):  
United Kingdom ◽  
Common Carp ◽  
Ornamental Fish ◽  
Significant Morbidity ◽  
Fact Sheet ◽  
Koi Herpesvirus ◽  
The United Kingdom ◽  
Aquatic Sciences ◽  
Almost All ◽  
Fish Industry

Koi herpesvirus (KHV) is a highly contagious virus that causes significant morbidity and mortality in common carp varieties. Common carp is raised as a foodfish in many countries and has also been selectively bred for the ornamental fish industry where it is known as koi. The first recognized case of KHV occurred in the United Kingdom in 1996. Since then other cases have been confirmed in almost all countries that culture koi and/or common carp with the exception of Australia. This 9-page fact sheet is intended to inform veterinarians, biologists, fish producers and hobbyists about KHV disease. Written by Kathleen H. Hartman, Roy P.E. Yanong, Deborah B. Pouder, B. Denise Petty, Ruth Francis-Floyd, Allen C. Riggs, and Thomas B. Waltzek, and published by the UF Department of Fisheries and Aquatic Sciences, April 2013. http://edis.ifas.ufl.edu/vm113

Charles de Gaulle and European Community

2020 ◽  
Vol 2020 (10-3) ◽  
pp. 70-81
Author(s):  
David Ramiro Troitino ◽  
Tanel Kerikmae ◽  
Olga Shumilo
Keyword(s):  
World War Ii ◽  
European Community ◽  
World War ◽  
Charles De Gaulle ◽  
The United Kingdom ◽  
History Of ◽  
The Common ◽  
Post War ◽  
De Gaulle

This article highlights the role of Charles de Gaulle in the history of united post-war Europe, his approaches to the internal and foreign French policies, also vetoing the membership of the United Kingdom in the European Community. The authors describe the emergence of De Gaulle as a politician, his uneasy relationship with Roosevelt and Churchill during World War II, also the roots of developing a “nationalistic” approach to regional policy after the end of the war. The article also considers the emergence of the Common Agricultural Policy (hereinafter - CAP), one of Charles de Gaulle’s biggest achievements in foreign policy, and the reasons for the Fouchet Plan defeat.

IMMl N'OHISTOPATHOLOCfCAL EFFECTS OF FENTHION TOXICITY ON THE COMMON CARP \CYPRIM'S CARPIO)

2005 ◽  
Vol 9 (1) ◽  
pp. 185-202
Author(s):  
Hekmat Tantauy ◽  
Mariam Sharaf ◽  
Ismail Abd Elnnbi ◽  
Hend Tag
Keyword(s):  
Common Carp ◽  
The Common

Marine Quality Standards: Their Derivation and Application in the United Kingdom

1985 ◽  
Vol 17 (6-7) ◽  
pp. 1187-1198 ◽  
Author(s):  
G. Mance ◽  
A. R. O'Donnell
Keyword(s):  
Coastal Waters ◽  
Sublethal Effects ◽  
Quality Standards ◽  
Toxicity Data ◽  
Marine Life ◽  
Nickel Zinc ◽  
The United Kingdom ◽  
The Common ◽  
Lead Nickel ◽  
The Impact

This paper discusses the derivation of environmental quality standards for coastal waters and the difficulties of using such standards for controlling industrial discharges. Attention is focused on the common List II substances, copper, chromium, lead, nickel, zinc and arsenic - and their effects on marine life. The adequacy of existing toxicity data is discussed and it is concluded that long exposure tests are required to provide information on sublethal effects. Such data are currently limited. It is also important that consideration be given to the effects that reducing salinities and increasing temperatures have in increasing the toxicity of these substances. The complexity of interpreting the results of laboratory toxicity data to coastal waters is discussed with reference to a study of the impact of an industrial discharge.

scholarly journals From Norms to Facts: The Realization of Rights in Common and Civil Private Law

2011 ◽  
Vol 56 (1) ◽  
pp. 77-114 ◽  
Author(s):  
Helge Dedek
Keyword(s):  
Legal System ◽  
Deep Structure ◽  
Common Law ◽  
Social Reality ◽  
Judicial Decision Making ◽  
Legal Traditions ◽  
The United Kingdom ◽  
The Common ◽  
Legal Concepts ◽  
The Ideal

Every legal system that ties judicial decision making to a body of preconceived norms has to face the tension between the normative formulation of the ideal and its approximation in social reality. In the parlance of the common law, it is, more concretely, the remedy that bridges the gap between the ideal and the real, or, rather, between norms and facts. In the common law world—particularly in the United Kingdom and the Commonwealth—a lively discourse has developed around the question of how rights relate to remedies. To the civilian legal scholar—used to thinking within a framework that strictly categorizes terms like substance and procedure, subjective right, action, and execution—the concept of remedy remains a mystery. The lack of “remedy” in the vocabulary of the civil law is more than just a matter of attaching different labels to functional equivalents, it is the expression of a different way of thinking about law. Only if a legal system is capable of satisfactorily transposing the abstract discourse of the law into social reality does the legal machinery fulfill its purpose: due to the pivotal importance of this translational process, the way it is cast in legal concepts thus allows for an insight into the deep structure of a legal culture, and, convergence notwithstanding, the remaining epistemological differences between the legal traditions of the West. A mixed jurisdiction must reflect upon these differences in order to understand its own condition and to define its future course.

scholarly journals Europe Abhors Donald Trump: The Opinion on the 2020 U.S. Presidential Elections and Their Candidates in the European Newspapers

2021 ◽  
pp. 000276422110055
Author(s):  
Marçal Sintes-Olivella ◽  
Pere Franch ◽  
Elena Yeste-Piquer ◽  
Klaus Zilles
Keyword(s):  
Qualitative Methodology ◽  
Election Campaign ◽  
Donald Trump ◽  
Presidential Debates ◽  
White House ◽  
The United Kingdom ◽  
European Public ◽  
The Media ◽  
The Common ◽  
The U.S

What is the opinion held by the European press on the U.S. election campaign and the candidates running for president? What are the predominant issues that attract the attention of European print media? Does Europe detest Donald Trump? The objective of the present study is to analyze the perception European commentators had of the 2020 race for the White House. The media, the audience, and European governments were captivated more than ever before by how the U.S. election campaign unfolded, fixing their gaze on the contest between Donald Trump and Joe Biden. Through a combined quantitative and qualitative methodology, a combination of content analysis and the application of framing theory (hitherto scarcely applied to opinion pieces), our research centers on exploring the views, opinions, and analyses published in eight leading newspapers from four European countries (France, Germany, Spain, and the United Kingdom) as expressed in their editorials and opinion articles. This study observes how the televised presidential debates were commented on, interpreted, and assessed by commentators from the eight newspapers we selected. The goal was to identify the common issues and frames that affected European public opinion on the U.S. presidential campaign and the aspirants to the White House.

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