scholarly journals Supplementation with Proline Improves Haemato-Biochemical and Reproductive Indicators in Male Rabbits Affected by Environmental Heat-Stress

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 373
Author(s):  
Sameh A. Abdelnour ◽  
Naif A. Al-Gabri ◽  
Nesrein M. Hashem ◽  
Antonio Gonzalez-Bulnes

Twenty-four adult rabbit bucks (n = 6 per treatment) were fed a basal diet supplemented with 0 (control), 50, 100, and 150 mg proline/kg dry matter (DM) diet for 12 weeks to determine possible usefulness for alleviating the negative impact of environmental heat stress on redox status, haemato-biochaemical attributes and semen quality. There were significant dose–response effects, with increments in levels of dietary proline (LDP) quadratically improving red blood cell counts (p = 0.017), rectal temperature (p = 0.009), and respiratory rate (p < 0.001). Increasing LDP cubically affected superoxide dismutase activity in blood plasma (p = 0.012) and total antioxidant capacity in both blood and seminal plasma (p < 0.001 and p = 0.006, respectively). The optimal response was observed at 30 and 80 mg proline/kg DM for blood and seminal plasma, respectively. With regards to homeostasis indexes, increments in LDP cubically modified blood plasma concentrations of total protein (p = 0.002) and albumin (p < 0.001), with an optimal response found at 70 mg proline/kg DM. A linear relationship (p = 0.005) was also observed between LDP and blood plasma glucose concentrations, with the optimal response being found at 100 mg proline/kg DM. Increasing LDP also showed positive effects on reproductive traits, with quadratic increases in blood plasma testosterone and cortisol concentrations (p < 0.001; optimal responses at 50 and 60 mg proline/kg DM, respectively), a positive linear relationship with in libido, ejaculate volume, sperm concentration and total sperm count (p < 0.001 for all; optimal responses observed at 100 mg proline/kg DM) and a quadratic increase in total functional sperm fraction (p < 0.001; optimal response at 70 mg proline mg/kg DM). Hence, the optimal positive effects of dietary proline supplementation on redox status, blood metabolites, and reproductive traits of rabbit bucks may be achieved at 50–100 mg/kg DM.

Author(s):  
Abhradip Majumder ◽  
Manjula Thakur ◽  
Mukesh Bhakat ◽  
Manorama Saha ◽  
Tushar Kumar Mohanty ◽  
...  

The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals were supplemented with 0%, 25%, and 50% Cu and Zn above the basal diet in T1, T2 and T3 groups, respectively for 180 days. Quantitative and qualitative characteristics of semen, blood and seminal plasma antioxidant status, blood and semen minerals (Cu, Zn, Ca and Mn) were determined in experimental Murrah bulls. Semen ejaculate volume (mL) increased in T2 and T3 while sperm concentration (million/mL), intact acrosome (%), HOST reacted spermatozoa (%) increased in T3 group compared to T1 and T2 groups. No difference was observed in mass motility, pH, live spermatozoa (%) in semen sexual behaviour except dismounting time which was decreased in T3 than T1 and T2 (plessthan0.05). SOD activity in blood plasma and LPO activity decreased in seminal plasma in both T2 and T3 groups than T1, whereas catalase activity did not show any significant difference. Cu and Zn supplementation in T2 group improved plessthan0.05) Zn level in blood and seminal plasma and Cu level in blood plasma only, but not in seminal plasma. Therefore, it can be concluded that supplementation of Zn and Cu at 50% above the recommended levels of ICAR (2013) improved the qualitative and quantitative attributes of semen in Murrah bulls.


1983 ◽  
Vol 19 (1) ◽  
pp. 725-729 ◽  
Author(s):  
R. Claus ◽  
D. Schopper ◽  
H.-G. Wagner

1983 ◽  
Vol 107 (1) ◽  
pp. 95-103 ◽  
Author(s):  
M. Morisawa ◽  
K. Suzuki ◽  
H. Shimizu ◽  
S. Morisawa ◽  
K. Yasuda

Spermatozoa of freshwater Cyprinidae (goldfish, carp, crucian carp and dace) remained immotile when the semen was diluted in solutions of NaCl, KCl, mannitol or glucose iso-osmolar to the seminal plasma (300 mosmol kg-1). The spermatozoa became motile in media containing these solutes if the osmolality was lower than that of the seminal plasma, suggesting that motility is suppressed by the osmolality of the seminal plasma in the sperm duct and initiated by a decrease of osmolality upon spawning into fresh water. Potassium was a major component of seminal plasma, having a concentration 20–30 times higher than that in the blood plasma in goldfish and carp. Sodium concentration in seminal plasma was lower than that in blood plasma. Potassium increased viability and speed of sperm movement at a concentration below that in the seminal plasma, whereas sodium and the nonelectrolytes were less effective. Potassium released with spermatozoa at spawning may therefore stimulate motility which has already been initiated by the decrease of osmolality.


2006 ◽  
Vol 52 (6) ◽  
pp. 805-810 ◽  
Author(s):  
Taiki MATSUOKA ◽  
Hiroko IMAI ◽  
Sadaki ASAKUMA ◽  
Hirohide KOHNO ◽  
Yutaka FUKUI

AIDS ◽  
2008 ◽  
Vol 22 (13) ◽  
pp. 1677-1679 ◽  
Author(s):  
Anne-Geneviève Marcelin ◽  
Roland Tubiana ◽  
Sidonie Lambert-Niclot ◽  
Gilles Lefebvre ◽  
Stéphanie Dominguez ◽  
...  

2018 ◽  
Vol 72 (4) ◽  
pp. 195-203 ◽  
Author(s):  
Alexander Vladimirovich Ivanov ◽  
Valery Vasilʼevich Alexandrin ◽  
Alexander Alexandrovich Paltsyn ◽  
Edward Danielevich Virus ◽  
Ksenya Alexandrovna Nikiforova ◽  
...  

2020 ◽  
Vol 51 (3) ◽  
pp. 760-766
Author(s):  
Mustafa & Wasman

The present investigation was carried out to compare the impact of cinnamon and clove powders and their oil additives as anti-stressors on quails under heat stress which is one of the riskiest environmental conditions that influence poultry all over the world in general and Iraq in particular. For this purpose, 420 quails (5 weeks old) were chosen and randomly grouped into seven treatments with different treatments (T0: control (standard diet), T1: 2 g clove powder/kg diet, T2: 1 ml clove oil/kg diet, T3: 2 g cinnamon powder, T4: 1 ml cinnamon oil/kg diet, T5: 1 g clove powder+1 g cinnamon powder/kg diet, T6: 0.5 ml clove oil+0.5 ml cinnamon oil/kg diet) added to standard diet. The quails were fed with these diets for 17 weeks. The results indicated that adding clove, cinnamon, and their oils to quail diet under heat stress led to significantly (p<0.05) lower relative density of heat shock protein HSP40, HSP70, HSP90, the concentrations of (corticosterone hormone, MDA and CK oxidative stimulator enzymes in blood plasma) and heterophil-to-lymphocyte (H/L) ratio, however, the concentrations of antioxidant enzymes AOA, GPx, SOD and CAT in blood plasma were significantly (p<0.05) higher in all additive treatments compared with the control group. It was also seen that treatment with the oil additives resulted in higher improvement than the powder additives.


2011 ◽  
Vol 80 (3) ◽  
pp. 287-292 ◽  
Author(s):  
Márta Erdélyi ◽  
Mária Weber ◽  
Krisztián Balogh ◽  
Zsolt Ancsin ◽  
Miklós Mézes

The effect of feeding a diet naturally contaminated with deoxynivalenol (DON) (0-21 days: 1.50 mg·kg-1; 22-39 days: 1.54 mg·kg-1) was studied in 40 broiler chickens. Birds were divided into two groups fed a control diet and a diet contaminated with DON (n = 20 in each). Feed intake was measured daily and individual live weight weekly; daily weight gain and feed to gain ratio were calculated. Five animals from each group were euthanized on days 21and 39 when blood (blood plasma and red blood cell haemolysates) and liver samples were collected. Concentration of triglyceride, uric acid and glucose and activities of ALT, AST and LDH were measured in blood plasma. Indicators of lipid peroxide and glutathione redox status, malondialdehyde and reduced glutathione concentration and glutathione-peroxidase activity were measured in blood plasma, red blood cell haemolysates and liver homogenates. The low dose of DON did not cause difference in the production traits, but caused significantly lower concentration of uric acid and glucose, and significantly higher concentration of triglyceride in blood plasma on day 21. Enzyme activities in blood plasma did not differ significantly between the treatment groups. Among the markers of lipid peroxide and glutathione redox status, malondialdehyde content was significantly higher in liver homogenate on day 21 in the group fed with DON contaminated diet, but reduced glutathione content and glutathione peroxidase activity did not differ significantly between the treatment groups. The results showed that diet contaminated even with a low content of deoxynivalenol caused alterations in selected biochemical indicators of blood and liver of broiler chicken.


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