scholarly journals Pedigree and Molecular Analyses in the Assessment of Genetic Variability of the Polish Greyhound

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 353
Author(s):  
Małgorzata Goleman ◽  
Ireneusz Balicki ◽  
Anna Radko ◽  
Iwona Rozempolska-Rucińska ◽  
Grzegorz Zięba
Keyword(s):  
Genetic Variability ◽  
Litter Size ◽  
Gene Diversity ◽  
Population Based ◽  
Pedigree Analysis ◽  
Molecular Testing ◽  
Pedigree Data ◽  
Kinship Matrix ◽  
Average Litter Size ◽  
The Mean

The aim of the study was to assess the genetic variability of the Polish Greyhound population based on pedigree analysis and molecular DNA testing and to determine the degree of relatedness among individuals in the population. Pedigree data of 912 Polish Greyhounds recorded in pedigree books since they were opened for this breed were analyzed. For molecular testing, DNA was obtained from cheek swabs taken from 235 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat—STR) was used. The mean inbreeding determined for the Polish Greyhound population based on pedigree analyses was low and amounted to 11.8%, but as many as 872 individuals of the 912 dogs in the studied population were inbred. A total of 83 founders (at least one unknown parent) were identified, among which 27 founders had both unknown parents. Full-sibling groups consisted of 130 individuals, with a minimum and maximum litter size of 2 and 16, respectively. The average litter size was 5.969. Gene diversity calculated based on the mean kinship matrix was 0.862 and the population mean kinship was 0.138. The founder genome equivalent based on the mean kinship matrix was 3.61; the founder genome surviving level was 12.34; the mean Ne was estimated at 21.76; and the Ne/N ratio was 0.135. The FIS inbreeding coefficient for 21 STR was negative, and the mean FIS value for all loci had a low negative value (−0.018). These values suggest a low level of inbreeding in the examined breed as well as the avoidance of mating related animals.

Development of a Strain of Rabbits with Congenital Simple Nonsyndromic Coronal Suture Synostosis Part I: Breeding Demographics, Inheritance Pattern, and Craniofacial Anomalies

1994 ◽  
Vol 31 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Mark P. Mooney ◽  
H. Losken Wolfgang ◽  
Michael I. Siegel ◽  
Janice F. Lalikos ◽  
Albert Losken ◽  
...  
Keyword(s):  
Litter Size ◽  
Rabbit Model ◽  
Pedigree Analysis ◽  
Human Populations ◽  
Incomplete Penetrance ◽  
Coronal Suture ◽  
Inheritance Pattern ◽  
Average Litter Size ◽  
Midfacial Hypoplasia ◽  
Congenital Condition

The lack of an animal model of congenital coronal suture (CS) synostosis has prompted the widespread use of an experimental rabbit model using adhesive Immobilization of the CS. Such postnatal models have helped make significant scientific contributions but may still not fully represent all aspects of the human congenital condition. In the March 1993 issue of The Cleft Palate-Craniofacial Journal we reported a female rabbit born in our laboratory with complete bilateral CS synostosis. This follow-up study presents our attempts to breed this animal and establish a strain of cranlosynostotic rabbits. To date, we have accomplished 10 back- and intercrosses with these animals and have produced a total of 71 live offspring; 10 animals exhibited complete nonsyndromic unilateral (plagiocephalic) or bilateral (brachycephalic) CS synostotic deformities at birth, and 19 animals exhibited partial CS synostosis that showed more than 75% growth retardation across the CS (well below the 95% confidence interval for normals). Results revealed that gestational time and litter size averages were consistent with those reported for the strain, although the average litter size decreased with increased inbreeding. By 1.5 weeks of age the completely synostosed animals already exhibited brachycephalic cranial vaults and midfacial hypoplasia compared to unaffected siblings. Initial pedigree analysis suggested an autosomal dominant inheritance pattern with incomplete penetrance and variable expressivity. The development of such a congenital rabbit model may prove useful In helping to understand the etiopathogenesis of this condition In human populations.

scholarly journals Production systems of swine in the rural areas of Rangamati and Khagrachari districts of Bangladesh

2012 ◽  
Vol 40 (1-2) ◽  
pp. 28-33
Author(s):  
ME Hossain ◽  
S Chakma ◽  
MM Khatun ◽  
M Hasanuzzaman ◽  
MY Miah ◽  
...  
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Rural Areas ◽  
Economic Condition ◽  
Production Systems ◽  
Economic Benefits ◽  
Disease Prevalence ◽  
Pig Production ◽  
Average Litter Size ◽  
The Mean

The study was undertaken for a period of 60 days to investigate the production systems of swine in Rangamati and Khagrachari districts, Bangladesh. Production systems, particularly housing, feeding, breeding, disease prevalence, vaccination, bio-security, marketing, socio-economic condition and constraints of pig production were investigated during the study period. It was found that the propensity of rearing pig differed significantly (P<0.01) among the pig owners. Pigs were reared mostly by poor and landless peoples (54.7%) followed by marginal (32.1%), medium (9.4%) and large (3.8%). Rearing systems were also different (P<0.01) and the mean figures were 43.4% for free range, 24.5% for tin shed housing , 20.8% for fencing and 11.3% for girth tethering systems. The average litter size, birth weight, post-weaning weight and weaning period were 9.3, 1.72 kg, 9.0 kg and 40.8 days respectively. Prevalence of diseases differed (P<0.01) and most prevalent diseases were diarrhea (35.8%), coccidiosis (20.8%), pneumonia (17.0%) and hemorrhagic septicemia (13.2%). The economic benefits generated from farming were selling of piglets.DOI: http://dx.doi.org/10.3329/bjas.v40i1-2.10787Bang. J. Anim. Sci. 2011. 40 (1-2): 28-33

scholarly journals Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Salvatore Bordonaro ◽  
Anna Maria Guastella ◽  
Andrea Criscione ◽  
Antonio Zuccaro ◽  
Donata Marletta
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variability ◽  
Genetic Information ◽  
Gene Diversity ◽  
Genetic Distances ◽  
Conservation Strategies ◽  
Population Structure Analysis ◽  
The Mean ◽  
Differentiation Index

The genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard the Pantesco breed as well as to manage Ragusano and Grigio Siciliano. In the whole sample, that included 108 donkeys representative of the three populations, a total of 85 alleles were detected. The mean number of alleles was lower in Pantesco (3.7), than in Grigio Siciliano and Ragusano (4.4 and 5.9, resp.). The three breeds showed a quite low level of gene diversity (He) ranging from 0.471 in Pantesco to 0.589 in Grigio. The overall genetic differentiation index (Fst) was quite high; more than 10% of the diversity was found among breeds. Reynolds’ () genetic distances, correspondence, and population structure analysis reproduced the same picture, revealing that, (a) Pantesco breed is the most differentiated in the context of the Sicilian indigenous breeds, (b) within Ragusano breed, two well-defined subgroups were observed. This information is worth of further investigation in order to provide suitable data for conservation strategies.

An isozyme-based assessment of the genetic variability within the Daucus carota complex (Apiaceae: Caucalideae)

1990 ◽  
Vol 68 (11) ◽  
pp. 2449-2457 ◽  
Author(s):  
Michel D. St. Pierre ◽  
Randall J. Bayer ◽  
I. Michael Weis
Keyword(s):  
Genetic Variability ◽  
Daucus Carota ◽  
Gene Diversity ◽  
Genetic Bottleneck ◽  
Enzyme Electrophoresis ◽  
Morphological Divergence ◽  
Simple Pattern ◽  
Isozyme Electrophoresis ◽  
The Mean ◽  
Eurasian Species

Daucus carota sensu lato is a phenotypically variable Eurasian species complex that has been naturalized and cultivated worldwide. One hundred and sixty-eight accessions from 32 countries were surveyed by enzyme electrophoresis to determine the extent of genetic variation present in the species. Eight enzyme systems coded by 16 putative loci were surveyed. They indicate that the recognized subspecies have diverged only slightly from one another with respect to the enzymes surveyed. The samples of wild taxa are more genetically diverse than those of the cultivated taxa, but not significantly different with respect to the mean number of alleles per locus, the proportion of polymorphic loci, the observed and expected heterozygosities, or Nei's genetic diversity statistics (P > 0.05). Members of the aggregate group gingidium have significantly lower total gene diversity within a taxon (P <.05) than do members of the aggregate group carota, as well as lower gene diversity within populations of the taxa (P < 0.01). Thus allozyme divergence is concordant with morphological divergence. Cultivars with carotene-pigmented roots show negligible amounts of genetic divergence. They appear to have evolved in a simple pattern after an initial genetic bottleneck. Key words: Daucus carota, isozyme, electrophoresis, genetic variability.

scholarly journals Pedigree analysis of 220 almond genotypes reveals two world mainstream breeding lines based on only three different cultivars

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Felipe Pérez de los Cobos ◽  
Pedro J. Martínez-García ◽  
Agustí Romero ◽  
Xavier Miarnau ◽  
Iban Eduardo ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Tree Breeding ◽  
Inbreeding Coefficient ◽  
Genetic Contribution ◽  
Pedigree Data ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Inbreeding Coefficients ◽  
The Usa ◽  
The Mean

AbstractLoss of genetic variability is an increasing challenge in tree breeding programs due to the repeated use of a reduced number of founder genotypes. However, in almond, little is known about the genetic variability in current breeding stocks, although several cases of inbreeding depression have been reported. To gain insights into the genetic structure in modern breeding programs worldwide, marker-verified pedigree data of 220 almond cultivars and breeding selections were analyzed. Inbreeding coefficients, pairwise relatedness, and genetic contribution were calculated for these genotypes. The results reveal two mainstream breeding lines based on three cultivars: “Tuono”, “Cristomorto”, and “Nonpareil”. Descendants from “Tuono” or “Cristomorto” number 76 (sharing 34 descendants), while “Nonpareil” has 71 descendants. The mean inbreeding coefficient of the analyzed genotypes was 0.041, with 14 genotypes presenting a high inbreeding coefficient, over 0.250. Breeding programs from France, the USA, and Spain showed inbreeding coefficients of 0.075, 0.070, and 0.037, respectively. According to their genetic contribution, modern cultivars from Israel, France, the USA, Spain, and Australia trace back to a maximum of six main founding genotypes. Among the group of 65 genotypes carrying the Sf allele for self-compatibility, the mean relatedness coefficient was 0.125, with “Tuono” as the main founding genotype (24.7% of total genetic contribution). The results broaden our understanding about the tendencies followed in almond breeding over the last 50 years and will have a large impact into breeding decision-making process worldwide. Increasing current genetic variability is required in almond breeding programs to assure genetic gain and continuing breeding progress.

scholarly journals Genetic diversity assessment of an indigenous horse population of Greece

2017 ◽  
Vol 33 (1) ◽  
pp. 81-90
Author(s):  
George Laliotis ◽  
Meni Avdi
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Pedigree Data ◽  
Horse Population ◽  
Heterozygote Deficit ◽  
Diversity Assessment ◽  
The Mean ◽  
North Western ◽  
Insular Region ◽  
Of Greece

Highly endangered local breeds are considered important not only for the maintenance of their genetic diversity for future survival but also because they regarded as part of the cultural heritage of the local and national communities. Using pedigree data and an analysis of 18 microsatellite loci we investigated the genetic diversity of a private (commercial) indigenous Skyros horse population, reared in an insular region of North-Western of Greece. The overall average animal inbreeding value reached 24%. Concerning average inbreeding value over non founding animals, it was estimated to 0.013, while the corresponding value over inbred animals were 0.13.The mean number of alleles per locus amounted to 3.72, ranging between 1 and 7 alleles. The average observed heterozygosity was 0.57. Taking into account the inbreeding estimated index, an average heterozygote deficit (Fis) of -0.09 was noted (P<0.05). Although the population maintained reasonable levels of genetic diversity, well studied inbreeding strategies should be implemented, in order to reduce the loss of genetic variability, to avoid extinction and further genetic drift of the population.

scholarly journals The reasons for low intrapopulation genetic variation in Lamium incisum Willd

2011 ◽  
Vol 60 (1) ◽  
pp. 79-84
Author(s):  
Katarzyna Wąsowicz ◽  
Monika Szczecińska ◽  
Jakub Sawicki
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Diversity Index ◽  
Gene Diversity ◽  
Weed Species ◽  
Preliminary Screening ◽  
Low Genetic Diversity ◽  
The Mean ◽  
Intrapopulation Genetic Variation ◽  
Issr Primers

The reasons for low intrapopulation genetic variation in Lamium incisum Willd The paper presents results of a study which aim was preliminary screening of intrapopulation genetic variability in Lamium incisum Willd. This weed species is rarely distributed in Poland and lessening its count during the last years. As a plant inhabiting anthropogenic sites it is exposed to extreme conditions and disturbances caused mostly by the progressive intensification of agriculture. In order to investigate the genetic variability of the selected population markers of ISSR category were used. The analysis of chosen individuals with use of three ISSR primers revealed total of 49 loci, of which only 15 were polymorphic. Nei's gene diversity index (HE=0.099) and the mean number of alleles per locus (AE=1.160) indicated low genetic diversity within the examined population. The research presented in this paper allows for a better learning of the genetic variability of the investigated species and considers probable factors influencing its level.

scholarly journals Genetic Variability and Population Structure of Polish Konik Horse Maternal Lines Based on Microsatellite Markers

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 546
Author(s):  
Agnieszka Fornal ◽  
Katarzyna Kowalska ◽  
Tomasz Zabek ◽  
Agata Piestrzynska-Kajtoch ◽  
Adrianna D. Musiał ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Microsatellite Markers ◽  
Pedigree Data ◽  
Horse Breed ◽  
K Value ◽  
Information Index ◽  
Genetic Clusters ◽  
The Mean ◽  
Index Value ◽  
F Statistics

The aim of the conservation programme is to maintain the population size of endangered livestock breeds of less economic importance at a level that ensures the survival of the breed, the preservation of genetic diversity, and the preservation of as many pedigree lines as possible. The Polish Konik, a native Polish primitive-type horse breed and is one of the breeds included in such a programme in Poland. Presently, there are only 16 (of the 35 maternal lines known in 1962), some of which are endangered. We examined the genetic variability and structure of the Polish Konik maternal lines (176 individuals) on the basis of the pedigree data and 17 microsatellite markers (STRs) from parentage testing. The overall mean number of alleles was 7.647 (±0.411), the effective number of alleles was 3.935 (±0.271), the mean number of alleles for which the frequency was equal to or lower than 5% was 4.471 (±0.286), and the mean information index was 1.506 (±0.087). The structure of the population and admixture patterns were calculated with the Structure and Structure Harvester software. The structural analysis indicated three likely genetic clusters; as the most optimal K value was estimated as 3, with ∆K of 15.4188. The F-statistics results indicated a low level of inbreeding (average inbreeding coefficient FIT was 0.0188, coefficient of differentiation FST was 0.0304, and mean inbreeding index value FIS was −0.0119). Variability monitoring should be carried out in order to avoid inbreeding depression, while breeding strategies should be designed to prevent the decrease of genetic variability in the Polish horse breed and to sustain the active female lines.

Genetic Variability of Bharat Merino Sheep Derived from Pedigree Information

2020 ◽  
Author(s):  
P. K. Mallick ◽  
I. Chauhan ◽  
S. M.K. Thirumaran ◽  
R. Pourouchttamane ◽  
Arun Kumar
Keyword(s):  
Genetic Diversity ◽  
Body Weight ◽  
Genetic Variability ◽  
Pedigree Analysis ◽  
Inbreeding Coefficient ◽  
Production Performance ◽  
Effective Number ◽  
Pedigree Data ◽  
Breeding Values ◽  
Merino Sheep

Background: The genetic variability in a population is the raw material for selection, because the estimation of genetic parameters depends on the variability present within the population. The pedigree analysis is a method to assess population genetic variability. An increase in the level of inbreeding disturbs the production performance of the animals. Hence, it is essential to assess the effect of inbreeding on production performance of the animals at regular intervals. The present study was conducted on data of Bharat Merino (BM) sheep with twin objectives of evaluating the population structure by pedigree analysis and possible effect of inbreeding on lamb growth and heritability estimates. Methods: The study was conducted on data consisting of a total of 9688 pedigree records of BM sheep born from 1975 to 2018 (43 years), out of which 9050 formed population reference (with both the parents known). ENDOG ver 4.8 program was used to generate different measures of genetic diversity. General Linear Model of SPSS 25.0 was used to ascertain the effect of inbreeding (Fi) or change in inbreeding (ÄFi) on the lamb live weights. Using animal model with software WOMBAT, single trait linear mixed model analyses were performed. The heritability estimates and breeding values were obtained by including or excluding the inbreeding coefficient in the model to observe how the estimates of heritability varied with inclusion or exclusion of the inbreeding coefficient. Result: Effective number of founders (fe) was 56, constituting 11.39% founders in the population reference, while the effective number of ancestors (fa) was 43.The genetic contribution of the 15 most influent ancestors explained 50% of the genetic variability in the dataset. The ratio fe/fa, representing the effect of population bottleneck, was 1.302. The average inbreeding coefficients for the whole pedigree was 2.36%, while it was 3.84% for inbred animals. It was found that the inbreeding coefficient (Fi) increased with the addition of each generation to the pedigree. The average relatedness coefficient was 4.53% between members of the population.The effect of individual inbreeding (Fi) or the change in inbreeding (DFi) was not significant on the lamb live weights, except the effect of individual inbreeding (Fi) on three-month body weight and average daily gain (0-3month) and of change in inbreeding (DFi) on three-month body weight. From the analysis of the pedigree data of Bharat Merino sheep, it was found that the most of the measures of genetic diversity were within acceptable limits and the pedigree data was reasonably well maintained. When inbreeding was accounted for in the model, there were reductions in h2 estimates as well as the estimates of breeding values for both 3WT and ADG1 and consequently there were changes in ranking of animals for both 3WT and ADG1.

scholarly journals Duroc boars have lower progeny mortality and lower fertility than Pietrain boars1

2019 ◽  
Vol 3 (2) ◽  
pp. 885-892
Author(s):  
Marie Louise M Pedersen ◽  
Ingela H Velander ◽  
Mai Britt F Nielsen ◽  
Nils Lundeheim ◽  
Bjarne Nielsen
Keyword(s):  
Litter Size ◽  
Artificial Insemination ◽  
Suckling Period ◽  
Average Litter Size ◽  
Slaughter Pigs ◽  
Lower Fertility ◽  
The Mean ◽  
Piglet Mortality ◽  
Terminal Sire ◽  
Size At Birth

Abstract In pig production, Pietrain and Duroc lines are often used as terminal sire lines to produce crossbred slaughter pigs. The objective of this study was to identify the differences in paternal fertility and mortality during the suckling period of crossbred progeny from Pietrain and Duroc terminal sire lines. In total, 87 purebred Duroc boars and 68 purebred Pietrain boars were used as terminal sires to produce 1,823 crossbred Duroc litters (D-litters) and 1,705 crossbred Pietrain litters (P-litters) in two production herds. The sows were crosses between DanBred Landrace and Yorkshire (F1). All boars were kept at the same artificial insemination (AI) station, and all semen doses were produced in the same laboratory. The experiment was balanced according to herd, boars, and time, with approximately 13 sows from each herd mated to each boar within each breed. The results showed higher fertility expressed as litter size at birth in P-litters compared with D-litters led to 0.5 higher total number born (TNB) for P-litters (P = 0.0076). However, piglet mortality including number of stillborn piglets was lower in D-litters compared with P-litters (P &lt; 0.0001), and 5 d after farrowing, the average litter size in P-litters ranged 0.4 below the litter size in D-litters (P &lt; 0.027). At 21 d after birth, mean litter size in P- and D-litters were 14.5 and 14.9 piglets per litter, respectively (P &lt; 0.015). This indicated that Pietrain progenies were weaker than Duroc progenies, and it was concluded that use of Duroc boars as the terminal sire line led to lower piglet mortality. In the two herds, the mean piglet mortality rate including still born piglets ranged from 19.5% to 23.6% and from 17.6% to 19.1% in P- and D-litters, respectively.

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