scholarly journals Observational Study on Variation of Longitudinal Platelet Counts in Calves over the First 14 Days of Life and Reference Intervals from Cross-Sectional Platelet and Leukocyte Counts in Dairy Calves up to Two Months of Age

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 347
Author(s):  
Emma Strous ◽  
Arne Vanhoudt ◽  
Anja Smolenaars ◽  
Gerdien van Schaik ◽  
Matthijs Schouten ◽  
...  
Keyword(s):  
Platelet Count ◽  
Observational Study ◽  
Reference Intervals ◽  
Dairy Calves ◽  
Cross Sectional ◽  
Adult Cattle ◽  
Platelet Counts ◽  
Holstein Friesian ◽  
Leukocyte Counts ◽  
Count Reference

Platelet and leukocyte count reference intervals (RIs) for cattle differ by age and while adult RIs are known, RIs for calves are studied less. The aims of this observational study are to evaluate variation of platelet counts of Holstein Friesian calves over the first 14 days of life and to propose RIs for platelet and leukocyte counts of Holstein Friesian calves aged 0–60 days. In a longitudinal study, 19 calves were blood sampled 17 times, in the first 14 days of their lives. Blood was collected in a citrate blood tube and platelet counts were determined. We assessed the course of platelet counts. In a field study, 457 healthy calves were blood sampled once. Blood was collected in an EDTA blood tube and platelet and leukocyte counts were determined. The RIs were calculated by the 2.5 and 97.5 percentiles. Platelet counts started to increase 24 h after birth (mean platelet count 381 × 109/L ± 138 × 109/L) and stabilized after five days (mean platelet count 642 × 109/L ± 265 × 109/L). In calves up to six days of age, platelet counts were lower than in calves older than five days. In conclusion, the RIs of platelet and leukocyte counts in calves were wider in range than the RIs for adult cattle, therefore, calf specific RIs for platelet and leukocyte counts should be used. From 6 until 60 days of age, we propose an RI for platelet counts of 287–1372 × 109/L and for the first 60 days of life an RI for leukocyte counts of 4.0–18.9 × 109/L.

Studies on Reference Intervals for Platelet Counts in Three Cities in China and one in Japan

2002 ◽  
Vol 88 (07) ◽  
pp. 111-114 ◽  
Author(s):  
Changgeng Ruan ◽  
Yun Wu ◽  
Tokuhiro Okada ◽  
Shigemi Motoi ◽  
Tamiaki Kondo ◽  
...  
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Healthy Subjects ◽  
Hematological Parameters ◽  
Reference Interval ◽  
Reference Intervals ◽  
Sichuan Province ◽  
Jiangsu Province ◽  
Platelet Volume ◽  
Platelet Counts

SummaryHematological parameters including platelet counts, etc. were determined in 1,140 healthy subjects living in four cities: Suzhou (Jiangsu Province), Chengdu (Sichuan Province) and Harbin (Heilongjang Province) in China, and Kobe in Japan. Then, the reference intervals for platelet counts were calculated and compared. The reference interval for platelet count of subjects aged between 18 and 60 years was 60-259 × 109/L in Suzhou and 52–202 × 109/L in Chengdu, and subjects with platelet counts of 100 × 109/L or less accounted for about 30% of the subjects examined in these cities. The reference intervals in Harbin and Kobe were within the range of 150–350 × 109/L, and no subject having a platelets count of 100 × 109/L or less was detected. Mean platelet volume (MPV) determined concurrently was negatively correlated with platelet count, and the reference intervals for MPV in Chengdu and Suzhou were higher than those in Harbin and Kobe.

scholarly journals Association between platelet count and osteoarthritis in women older than 50 years

2020 ◽  
Vol 12 ◽  
pp. 1759720X2091286
Author(s):  
Yu-Jin Kwon ◽  
Il-Hyun Koh ◽  
Kwangho Chung ◽  
Yong-Jae Lee ◽  
Hyoung-Sik Kim
Keyword(s):  
Platelet Count ◽  
Confidence Intervals ◽  
Multiple Logistic Regression Analysis ◽  
Odds Ratios ◽  
Characteristic Analysis ◽  
Cross Sectional ◽  
Platelet Counts ◽  
Health And Nutrition ◽  
Inflammatory Processes ◽  
The Relationship

Background: Osteoarthritis (OA) is a multifactorial disease involving inflammatory processes. Platelets play important roles in both hemostasis and the inflammatory response; however, the relationship between platelet count and OA is unclear. Our aim was to evaluate the association between platelet count and knee and hip OA in Korean women. Methods: In this cross-sectional designed study, we included a total of 6011 women aged ⩾50 years from the 2010–2013 Korea National Health and Nutrition Examination Survey. Knee and hip OA were defined as Kellgren–Lawrence grade ⩾2 and presence of knee or hip pain, respectively. Platelet counts were divided into quartiles as follows: Q1, 150–212 (103/µl); Q2, 213–246 (103/µl); Q3, 247–283 (103/µl); and Q4, 284–450 (103/µl). Multiple logistic-regression analysis was conducted to calculate odds ratios and 95% confidence intervals. Receiver operating characteristic analysis was performed to determine the optimal platelet count cut-off with which to discriminate participants with knee and/hip OA versus those without OA. Results: Of the 6011 participants, 1141 (18.1%) had knee or hip OA. The mean age of participants without OA was 60.6 years, and that of participants with OA was 68.0 years. Compared with the lowest quartile, odds ratios (95% confidence intervals) for OA were 1.08 (0.84–1.39) for Q2, 0.94 (0.73–1.23) for Q3, and 1.35 (1.08–1.69) for Q4 after adjusting for confounders. The prevalence of OA was significantly higher with platelet counts ⩾288 × 103/µl, compared with platelet counts <288 × 103/µl. Conclusion: High platelet counts within the normal range are significantly associated with knee and hip OA.

An evaluation of methods for determining reference intervals for light transmission platelet aggregation tests on samples with normal or reduced platelet counts

2008 ◽  
Vol 100 (07) ◽  
pp. 01-12 ◽  
Author(s):  
Karen A. Moffat ◽  
Menaka Pai ◽  
Yang Liu ◽  
Jodi Seecharan ◽  
Heather McKay ◽  
...  
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Platelet Function ◽  
Light Transmission ◽  
Reference Intervals ◽  
Platelet Counts ◽  
Healthy Control ◽  
Parametric Analyses ◽  
Control Samples ◽  
Non Parametric

SummaryLight transmission platelet aggregation tests are important for diagnosing platelet function defects. However, uncertainties exist about the best procedures to determine aggregation reference intervals. We investigated methods for determining reference intervals for light transmission aggregation tests, using the % maximal aggregation values for prospectively collected data on healthy control samples. Reference intervals for samples tested at 250 x 109 platelets/l were determined by mean ± 2 standard deviations and non-parametric analyses. To establish reference intervals for tests on thrombocytopenic subjects, regression analyses were used to estimate 95% confidence limits for % maximal aggregation, according to sample platelet counts, using data for control samples diluted to match the platelet count of undiluted thrombocytopenic patient platelet-rich plasma samples. For samples tested at 250 x 109 platelets/l, non-parametric analyses described 95% of data for healthy control samples better than mean ± 2 standard deviations. For samples tested at lower counts, to match thrombocytopenic samples, the % maximal aggregation was influenced by platelet count and derived limits were wider at very low platelet counts for almost all agonists. With ristocetin, it proved feasible to test samples with very low platelet counts to exclude Bernard-Soulier syndrome and type 2B von Willebrand disease. Non-parametric analyses should be the preferred method to establish light transmission aggregation reference intervals for samples tested at normal platelet counts. The derived limits for thrombocytopenic samples provide guidance for evaluating thrombocytopenic platelet function disorders, including which agonists to test, based on the sample platelet count.

scholarly journals The effect of age when group housed and other management factors on playing and non-nutritive sucking behaviour in dairy calves: a cross sectional observational study

2020 ◽  
Vol 62 (1) ◽  
Author(s):  
Masja Reipurth ◽  
Stephanie Kruuse Klausen ◽  
Matthew Denwood ◽  
Björn Forkman ◽  
Hans Houe
Keyword(s):  
Observational Study ◽  
Dairy Calves ◽  
Organic N ◽  
Age Difference ◽  
Cross Sectional ◽  
Play Behaviour ◽  
Nutritive Sucking ◽  
Indirect Measures ◽  
The Mean ◽  
First Time

Abstract Background The aim of this study was to investigate if calves’ play behaviour and non-nutritive sucking behaviour, as indirect measures of welfare status, are associated with the age of the calf when group housed, age when observed, age difference within the group, pen size, milk feeding system, current or previous sicknesses, access to dry teat, indoor/outdoor rearing, sex, organic/conventional farm, group size and regrouping events. An observational study was conducted on 176 Danish dairy calves in the age range of 1–12 weeks, on both conventional (n = 17) and organic (n = 5) farms. All calves had been group housed before 8 weeks of age and had spent various periods of time with the dam and/or individually housed before being group housed. Behaviour was recorded continuously by filming each individual calf over a period of 30 min. Results The calf’s age when group housed for the first time was not found to be significantly associated with duration of either play behaviour (P = 0.55) or non-nutritive sucking behaviour (P = 0.44). It was found that calves had significantly reduced odds of playing for longer than the mean play duration (5.5 s) for each day of their lives (OR = 0.97, P = 0.003). Also, they had reduced odds of performing non-nutritive sucking behaviour for longer than the mean non-nutritive sucking duration (145.5 s) when milk was allocated by drinker buckets fitted with a teat compared to by bowl or trough (OR = 0.06, P = 0.02). Conclusion No significant associations were found between calves’ age when group housed for the first time and play and non-nutritive sucking behaviour. It was found that calves’ play behaviour decreased with increasing age, and that non-nutritive sucking behaviour decreased when milk was allocated with a teat compared to no teat.

scholarly journals Occurrences of Eimeria spp. and gastrointestinal nematodes in dairy calves in southern Minas Gerais, Brazil

2012 ◽  
Vol 21 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Fábio Raphael Pascoti Bruhn ◽  
Fidelis Antônio Silva Júnior ◽  
André Henrique de Oliveira Carvalho ◽  
Débora Ribeiro Orlando ◽  
Christiane Maria Barcellos Magalhães da Rocha ◽  
...  
Keyword(s):  
Observational Study ◽  
Dairy Farms ◽  
Gastrointestinal Nematodes ◽  
Minas Gerais ◽  
Dairy Calves ◽  
Cross Sectional ◽  
Factors Associated ◽  
Eimeria Spp

The aim of this cross-sectional observational study was to determine the frequency and factors associated with infection by Eimeria spp. and gastrointestinal nematodes in 356 calves on 20 dairy farms located in southern Minas Gerais, Brazil. Ten species of Eimeria spp. were identified, of which E. bovis (37.6%) and E. zuernii (17.9%) were the most frequent. From fecal cultures, four genera of gastrointestinal nematodes were recovered, of which Cooperia spp. (74.6%) and Haemonchus (19.4%) were the most frequent. Variables relating to higher levels of technology used on dairy farms showed a significant association (p < 0.05) with higher OPG and EPG counts, and are discussed in this study.

scholarly journals BLOOD SMEAR PROFILE OF PATIENTS WITH DENGUE HEMORRHAGIC FEVER IN BALI ROYAL HOSPITAL

2021 ◽  
Vol 5 (1) ◽  
pp. 39
Author(s):  
I Made Dwi Mertha Adnyana ◽  
Ni Luh Gede Sudaryati ◽  
A.A Komang Suardana
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Blood Smear ◽  
Hemorrhagic Fever ◽  
Blood Component ◽  
Cross Sectional ◽  
Royal Hospital ◽  
Platelet Counts ◽  
Univariate Anova ◽  
Leukocyte Counts ◽  
Hematological Tests

Background: Each year, dengue hemorrhagic fever has grown. Clinical hematological examinations and blood preparations are used to confirm the diagnosis. Purpose: To detect, assess and characterize dengue hemorrhagic fever blood smear at Bali Royal Hospital. Method: Cross-sectional descriptive observational study-was conducted in April at Bali Royal Hospital on 37 patients with dengue hemorrhagic fever, clinical hematological tests and the manufacture of blood preparations identified using inclusion and exclusion criteria. Platelet counts, hemoglobin levels, hematocrit values, leukocyte counts, clinic degrees, gender and age, and blue plasma lymphocytes, are all used in hematological analyzed by univariate, ANOVA and BNT tests to analyze data. Result: Patients with dengue hemorrhagic fever are mostly toddlers and children (35.13%), women (54.0%), and dengue fever degree I (64.86%) with positive blue plasma lymphocytes (51.36%). Platelet counts, hemoglobin levels, hematocrit values, and leukocyte counts are significantly changed on days 3, 6, and 9, with probability values (p=0.000) less than p=0.01. The findings of the BNT test indicate that the number of platelets and haemoglobin on various days is significantly different (p<0.01), but the hematocrit value and leukocyte calculation are not significantly different (p>0.01). Conclusion: Clinical hematological tests and blood preparations reveal a blood component anomaly in Dengue Haemorragic Fever (DHF) patients at Bali Royal Hospital on days 3, 6, and 9.

scholarly journals A New, Automatic, Disposable System for Blood Counts and Hemoglobin

Blood ◽  
1963 ◽  
Vol 21 (5) ◽  
pp. 648-655 ◽  
Author(s):  
MELVIN H. FREUNDLICH ◽  
HORACE W. GERARDE
Keyword(s):  
Platelet Count ◽  
Test System ◽  
High Index ◽  
Platelet Counts ◽  
Leukocyte Counts ◽  
Blood Dilution ◽  
Disposable Plastic ◽  
New System

Abstract A new system of blood dilution which uses a self-filling, disposable micro-pipette and a prefilled, disposable plastic dilution container has been described. By this means a single .013 ml. sample of blood is all that is required to do a WBC, RBC, Hb and platelet count. Comparison of the system (Unopette-B-D) with standard pipette counts proved to have a high index of correlation as far as hemoglobin, erythrocyte counts and leukocyte counts were concerned. Platelet counts tended to show less agreement, the test system giving consistently lower results. The reasons for this were discussed. The advantages of the new system were discussed.

scholarly journals Identification of Shigatoxigenic and Enteropathogenic Escherichia coli Serotypes in Healthy Young Dairy Calves in Belgium by Recto-Anal Mucosal Swabbing

2020 ◽  
Vol 7 (4) ◽  
pp. 167
Author(s):  
Audrey Habets ◽  
Frederik Engelen ◽  
Jean-Noël Duprez ◽  
Brecht Devleesschauwer ◽  
Marc Heyndrickx ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intestinal Tract ◽  
Carrier State ◽  
Enterohemorrhagic Escherichia Coli ◽  
Dairy Calves ◽  
Adult Cattle ◽  
E Coli ◽  
Holstein Friesian ◽  
Agar Media ◽  
Pcr Targeting

Enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli (EPEC), and Shigatoxigenic E. coli (STEC) are carried by healthy adult cattle and even more frequently by young calves in their intestinal tract, especially at the height of the recto-anal junction. The purpose of the present study was to assess the presence of ten EHEC, EPEC, and/or STEC O serotypes (O5, O26, O80, O103, O111, O118, O121, O145, O157, and O165) in calves sampled via recto-anal mucosal swabs (RAMS) at three dairy farms in Belgium. A total of 233 RAMS were collected on three consecutive occasions from healthy <6-month-old Holstein-Friesian calves and submitted to a PCR targeting the eae, stx1, and stx2 genes after non-selective overnight enrichment growth. The 148 RAMS testing positive were streaked on four (semi-)selective agar media; of the 2146 colonies tested, 294 from 69 RAMS were PCR-confirmed as EHEC, EPEC, or STEC. The most frequent virulotype was eae+ EPEC and the second one was stx1+ stx2+ STEC, while the eae+ stx1+ and eae+ stx1+ stx2+ virulotypes were the most frequent among EHEC. The majority of EHEC (73%) tested positive for one of the five O serotypes detected (O26, O103, O111, O145, or O157) vs. 23% of EPEC and 45% of STEC. Similarly, more RAMS (73%) harbored EHEC isolates positive for those five serotypes compared to EPEC (53%) or STEC (52%). This survey confirms that (i) healthy young dairy calves are asymptomatic carriers of EHEC and EPEC in Belgium; (ii) the carrier state rates, the virulotypes, and the identified O serotypes differ between farms and in time; and (iii) a majority of EPEC belong to so far unidentified O serotypes.

scholarly journals Relationship between Cardiopulmonary Bypass Time, Platelet Count, Fibrinogen, ROTEM Measurements, Antithrombin Level, and Bleeding Amount during Cardiovascular Surgery and Intensive Care Unit Stay: A Observational Study

2021 ◽  
Author(s):  
Mitsuharu Kodaka ◽  
Junko Ichikawa ◽  
Kazuyoshi Ando ◽  
Makiko Komori
Keyword(s):  
Intensive Care ◽  
Platelet Count ◽  
Cardiopulmonary Bypass ◽  
Blood Loss ◽  
Observational Study ◽  
Medical Information ◽  
The Other ◽  
University Hospital ◽  
Platelet Counts ◽  
Perioperative Bleeding

Abstract Purpose:Hemoglobin levels after a cardiopulmonary bypass (CPB) are easily estimated; however, decreased fibrinogen and platelet levels before and after CPB are difficult to predict because of the adsorption and consumption. We hypothesized that fibrinogen levels, platelet count, and measurements by ROTEMTM may decrease in proportion to CPB time; moreover, we compared the perioperative blood loss by conducting a observational study. Methods:A total of 160 patients were enrolled and divided into three groups depending on the CPB time: < 2, 2–3, and >3 h. Blood samples were obtained at four time points, i.e., baseline, CPB start, CPB end, and intensive care, and platelet counts, ROTEM, fibrinogen, and antithrombin were measured. Results:A total of 74 patients were included in the <2 h, 63 in the 2–3 h, and 23 in the >3 h groups.No difference was observed in the platelet counts and fibrinogen between each group while weaning CPB, whereas the amplitude 10 min of EXTEM A10, FIBTEM A10, and antithrombin in the >3 h group were lower than those in the other groups. Furthermore, blood loss and each transfusion component were higher in the >3 h group than those in the other groups.Conclusions:The longer the CPB times, the greater perioperative bleeding loss and transfusion, particularly if >3 h, due to decreased (EXTEM A10 and FIBTEM A10) represented by ROTEMTM.Trial registration: This trial is registered with University hospital Medical Information Network (UMIN) Center, https://www.umin.ac.jp/. (ID: UMIN000017412) on May 5, 2015.

scholarly journals Phototherapy in full term neonates suffering from unconjugated hyperbilirubinemia; Does it affect platelet count?

2020 ◽  
Vol 27 (09) ◽  
pp. 1911-1915
Author(s):  
Adnan Bashir ◽  
Ashok Kumar ◽  
Nathumal Maheshwari ◽  
Bilawal Hingorjo ◽  
Shakeel Ahmed ◽  
...  
Keyword(s):  
Platelet Count ◽  
Blood Platelets ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Term Neonates ◽  
Platelet Counts ◽  
Medical College ◽  
Significant Difference ◽  
Chi Square Test

Objectives: Investigating the hazardous effect of Phototherapy on Platelet counts in healthy neonates with Neonatal hyperbilirubinemia (NH). Study Design: Cross Sectional study. Setting: Department of Paediatrics, SMBB Medical College Layari General Hospital, Karachi, Sindh. Period: January 2018 to May 2019. Material & Methods: A sample of 313 neonates suffering from NH was selected according to the inclusion and exclusion criteria. Blood Platelets were counted at baseline, and 24, 48 and 72 hours of phototherapy. Statistical software SPSS 21.0 (IBM, Incorp, USA) was used for data analysis using paired t-test and Pearson`s Chi- square test at P≤0.05. Results: Platelet counts at 24, 48 and 72 hours of phototherapy were found 2.97±0.45×109/L, 3.38±0.48×109/L and 3.52± 0.47×109/L respectively. Platelet counts at 24, 48 and 72 hours showed statistically significant difference compared to baseline counts (P<0.05). A statistically insignificant decline in total population platelet count after phototherapy was noted (P>0.05). However, thrombocytopenia was noted in 9 (2.87%) cases; comprising mild and moderate in 7 (2.23%) and 2 (0.64%) cases respectively. Conclusion: The present study observed thrombocytopenia in 9 (2.87%) of total cases; of which mild and moderate thrombocytopenia occurred in 7 (2.23%) and 2 (0.64%) cases respectively.

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