scholarly journals Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 262
Author(s):  
Simone Tamara Schabmeyer ◽  
Anna Maria Kneidl ◽  
Julia Katharina Schneider ◽  
Sandra Kirsch ◽  
Yury Zablotski ◽  
...  
Keyword(s):  
Gene Expression Analysis ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Interferon Α ◽  
Classical Pathway ◽  
Fatty Acid Binding ◽  
Type 1 Interferon ◽  
Interferon Receptor

The inadequate maternal recognition of embryonic interferon τ (IFNτ) might explain subfertility in cattle. This study aimed at modeling the inducibility of type 1 interferon receptor subunits 1/2 (IFNAR1/2), mimicking competition between IFNτ and infection-associated interferon α (IFNα), and simulating type 1 interferon pathways in vitro. Endometrial explants (n = 728 from n = 26 healthy uteri) were collected at the abattoir, challenged with IFNτ and/or IFNα in different concentrations, and incubated for 24 h. Gene expression analysis confirmed the inducibility of IFNAR1/2 within this model, it being most prominent in IFNAR2 with 10 ng/mL IFNα (p = 0.001). The upregulation of interferon-induced GTP-binding protein (MX1, classical pathway) was higher in explants treated with 300 ng/mL compared to 10 ng/mL IFNτ (p < 0.0001), whereas the non‑classical candidate fatty acid binding protein 3 (FABP3) exhibited significant downregulation comparing 300 ng/mL to 10 ng/mL IFNτ. The comparison of explants challenged with IFNτ + IFNα indicated the competition of IFNτ and IFNα downstream of the regulatory factors. In conclusion, using this well-defined explant model, interactions between infection-associated signals and IFNτ were indicated. This model can be applied to verify these findings and to mimic and explore the embryo–maternal contact zone in more detail.

scholarly journals Urinary Liver-Type Fatty Acid-Binding Protein and Progression of Diabetic Nephropathy in Type 1 Diabetes

Diabetes Care ◽  
2013 ◽  
Vol 36 (7) ◽  
pp. 2077-2083 ◽  
Author(s):  
N. M. Panduru ◽  
C. Forsblom ◽  
M. Saraheimo ◽  
L. Thorn ◽  
A. Bierhaus ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Diabetic Nephropathy ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil

Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 7
Author(s):  
Tiara Cascais-Figueiredo ◽  
Phelipe Austriaco-Teixeira ◽  
Maria Fantinatti ◽  
Maria Luciana Silva-Freitas ◽  
Joanna Reis Santos-Oliveira ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Low Income ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Developed Countries ◽  
Mucosal Barrier ◽  
Fatty Acid Binding ◽  
Epithelial Damage ◽  
Acid Binding Protein

Giardiasis is an intestinal infection caused by ingestion of water or food contaminated with cysts of Giardia lamblia. Susceptibility is higher in children and overall prevalence can reach up to 90% in low-income areas, although outbreaks are also reported in developed countries. Both parasite and immune-mediated epithelial damage has been observed in vitro and in animal models. However, whether enterocytes are directly damaged during infection is not entirely known. Our goal was to identify whether plasma levels of intestinal fatty acid binding protein (I-FABP), a marker of enterocyte damage, are related to the immune response in giardiasis. Blood plasma was collected from 31 children (19 Giardia-positive) from a public day care in Rio de Janeiro, Brazil. The levels of I-FABP were increased in Giardia-infected children compared to children without detectable infection. There was no difference in I-FABP levels in giardiasis caused by different genetic assemblages of Giardia. Levels of IL-8 were decreased, while there was a trend to elevated IL-17 in the Giardia-positive children. A positive correlation was observed between I-FABP and IL-17 levels as well as TNF, suggesting that epithelial damage can be related to cytokine production during giardiasis. These results help elucidate the relationship between the disruption of the intestinal mucosal barrier and immune responses to G. lamblia in children.

scholarly journals Fatty Acid-Binding Protein 4 (FABP4): Pathophysiological Insights and Potent Clinical Biomarker of Metabolic and Cardiovascular Diseases

2014 ◽  
Vol 8s3 ◽  
pp. CMC.S17067 ◽  
Author(s):  
Masato Furuhashi ◽  
Shigeyuki Saitoh ◽  
Kazuaki Shimamoto ◽  
Tetsuji Miura
Keyword(s):  
Insulin Resistance ◽  
Fatty Acid ◽  
Cardiovascular Diseases ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Ectopic Expression ◽  
Classical Pathway ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
And Function

Over the past decade, evidences of an integration of metabolic and inflammatory pathways, referred to as metaflammation in several aspects of metabolic syndrome, have been accumulating. Fatty acid-binding protein 4 (FABP4), also known as adipocyte FABP (A-FABP) or aP2, is mainly expressed in adipocytes and macrophages and plays an important role in the development of insulin resistance and atherosclerosis in relation to metaflammation. Despite lack of a typical secretory signal peptide, FABP4 has been shown to be released from adipocytes in a non-classical pathway associated with lipolysis, possibly acting as an adipokine. Elevation of circulating FABP4 levels is associated with obesity, insulin resistance, diabetes mellitus, hypertension, cardiac dysfunction, atherosclerosis, and cardiovascular events. Furthermore, ectopic expression and function of FABP4 in several types of cells and tissues have been recently demonstrated. Here, we discuss both the significant role of FABP4 in pathophysiological insights and its usefulness as a biomarker of metabolic and cardiovascular diseases.

scholarly journals Urinary liver-type fatty acid binding protein is an independent predictor of stroke and mortality in individuals with type 1 diabetes

Diabetologia ◽  
2017 ◽  
Vol 60 (9) ◽  
pp. 1782-1790 ◽  
Author(s):  
Nicolae M. Panduru ◽  
◽  
Carol Forsblom ◽  
Markku Saraheimo ◽  
Lena M. Thorn ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Independent Predictor ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Urinary liver-type fatty acid-binding protein as a Marker for Early Diagnosis of Diabetic Nephropathy in Type 1 Diabetic Children.

2018 ◽  
Vol 11 (5) ◽  
pp. 329
Author(s):  
Mones M Abushady ◽  
Gihan A Fathy ◽  
Inas R El-alameey ◽  
Abbas Ma ◽  
Essam M Galal ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Fatty Acid ◽  
Early Diagnosis ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Linear Regression Analysis ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
The Mean

 Objectives: Renal failure and premature mortality are fatal prognosis of diabetic nephropathy. To improve patient outcome, early diagnosis of diabetic nephropathy is necessary. The study was designed to evaluate urinary liver-type fatty acid binding protein (L-FABP), as an early biomarker of tubulointerstitial injury, and its association with the clinical characteristics of type 1 diabetic children.Methods: Fifty randomly selected patients with type 1 diabetes mellitus (DM) attending the diabetes outpatient clinic of Ain Shams University Children’s Hospital were included in the study. 50 age and sex-matched healthy subjects were enrolled as controls. Urinary L-FABP, 24 h urine albumin, hemoglobin A1c (HbA1c), serum creatinine, and lipid profile were measured.Results: Diabetic subjects had higher mean urinary L-FABP than controls (p<0.05). In microalbuminuric diabetic subjects, the mean urinary L-FABP was detected to be significantly higher than that in normoalbuminuric diabetic subjects, and significantly higher values of the mean urinary L-FABP were detected in the microalbuminuric and the normoalbuminuric subjects than the controls (p<0.05). Multiple linear regression analysis showed that duration of DM and HbA1c was the main predictors of urinary L-FABP in diabetic subjects.Conclusion: In patients with childhood-onset T1D, urinary L-FABP may be used as an indicator of renal injury in early stages of nephropathy, even in the normoalbuminuric state.

804 FATTY ACID BINDING PROTEIN 4 INHIBITOR INHIBITS PANCREATIC CANCER GROWTH IN VITRO AND IN VIVO.

2021 ◽  
Vol 160 (6) ◽  
pp. S-166
Author(s):  
Shuhei Shinoda ◽  
Keith Wirth ◽  
Sayeed Ikramuddin ◽  
David A. Bernlohr ◽  
Masato Yamamoto
Keyword(s):  
Pancreatic Cancer ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Cancer Growth ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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