scholarly journals Effects of Dietary N-Carbamylglutamate on Growth Performance, Apparent Digestibility, Nitrogen Metabolism and Plasma Metabolites of Fattening Holstein Bulls

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 126
Author(s):  
Jinshan Yang ◽  
Jian Zheng ◽  
Xinpeng Fang ◽  
Xin Jiang ◽  
Yukun Sun ◽  
...  

N-carbamylglutamate (NCG), a structural analog of N-acetylglutamate, improves nitrogen utilization in dairy cows. However, the effects of NCG on bulls are unknown. The purpose of the current research was to investigate the effects of adding different amounts of NCG on growth performance, nutrient digestibility, nitrogen metabolism and plasma metabolites of fattening Holstein bulls. Twenty-four Holstein bulls with similar body weights (BW, 408 ± 21.9 kg) and ages (450 ± 6.1 d; all mean ± SD) were selected for the feeding trial. After 2 weeks of adaptation, bulls were blocked by BW and age and subsequently randomly assigned to 1 of 4 groups: (1) CON group (control diet), (2) L group (supplementation with 20 mg/kg BW NCG), (3) M group (supplementation with 40 mg/kg BW NCG), or (4) H group (supplementation with 80 mg/kg BW NCG). The addition of NCG linearly and quadratically increased the average daily gain (CON vs. L vs. M vs. H = 1.03 vs. 1.19 vs. 1.40 vs. 1.26 kg/d) (p < 0.05), feed conversion ratio (CON vs. L vs. M vs. H = 11.92 vs. 9.22 vs. 7.76 vs. 8.62) (p < 0.05), crude protein digestibility (CON vs. L vs. M vs. H = 64.3 vs. 63.8 vs. 67.7 vs. 65.8%) (0.05 < p < 0.10), N retention (p < 0.05) and N utilization (p < 0.05) of bulls, whereas the contents of fecal N (0.05 < p < 0.10) and urinary N (0.05 < p < 0.10) in NCG-fed bulls linearly decreased compared with those in CON bulls. Bulls fed NCG showed a quadratic increased plasma nitric oxide (p < 0.05) concentration. Furthermore, Arg (p < 0.05), Ile (p < 0.05), Val (p < 0.05), Ala (p < 0.05), Glu (p < 0.05), Ser (p < 0.05), total essential amino acid (p < 0.05) and total nonessential amino acid (p < 0.05) concentrations linearly and quadratically increased with increasing doses of NCG. In contrast, plasma urea (p < 0.05) and ammonia (p < 0.05) concentration linearly and quadratically decreased with increasing doses of NCG. Overall, the addition of NCG increased plasma Arg, Ile, Val, TEAA and TNEAA concentration, which in turn resulted in a higher N utilization and, therefore, higher average daily gain in NCG-fed bulls, providing baseline data for the widespread application of NCG in beef cattle production.

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Eric Hatungimana ◽  
Tess C Stahl ◽  
Peter S Erickson

Abstract The objective of this study was to evaluate the growth performance and apparent total tract nutrient digestibility of Holstein heifers limit-fed diets containing different amounts of wet brewer’s grains (WBG). A 12-wk randomized complete block study was conducted using 30 yearling Holstein heifers [378 ± 27 d of age, and body weight (BW) of 357.8 ± 27.6 kg (mean ± SD)]. Treatments were 0%, 10% and 20% of WBG on a dry matter (DM) basis and diets were formulated to be limit-fed for dry matter intake (DMI) at 2.35% of BW and provided 15% crude protein (CP) and 2.27 Mcal metabolizable energy/kg of DM. Dry matter intake was recorded daily, while BW and skeletal measurements were measured every 2 wk. During week 12, fecal samples were collected directly from the rectum over four consecutive days and composited by heifer to determine apparent total tract nutrient digestibility using acid detergent insoluble ash as a marker. Data were analyzed using the MIXED procedure of SAS. Dry matter intakes, BW, and average daily gain were not different among treatments (P = 0.2, P = 0.4, and P = 0.6, respectively). Dry matter intakes ranged from 8.6 to 9.0 kg/d. Average BW were 404.4, 411.5, and 409.3 kg for heifers fed the 0%, 10%, and 20% WBG diets, respectively. Average daily gains were 1.03, 1.04, and 0.96 kg/d for heifers fed the 0%, 10%, and 20% WBG diets respectively. Skeletal measurements and body condition scores (BCS) were not different among treatments except for the change in heart girth (P &lt; 0.01) and initial BCS (P &lt; 0.01). Apparent total tract digestibilities of DM, organic matter, CP, fat, and hemicellulose were greater or tended to be greater in heifers fed 0% and 20% WBG treatments than heifers fed 10 % WBG (P = 0.04, P = 0.04, P = 0.06, P = 0.06, and P = 0.01, respectively). Neutral detergent fiber, acid detergent fiber, and fat digestibilities were similar among treatments (P = 0.2, P = 0.3, and P = 0.3, respectively). During the digestibility phase, DMI tended to be greater (P = 0.08) for the 10% WBG treatment. These results demonstrate that limit-feeding heifers with diets containing up to 20% WBG could replace soybean- and corn-based concentrates in diets without adverse consequences to the heifer growth performance.


2014 ◽  
Vol 59 (No. 11) ◽  
pp. 529-537 ◽  
Author(s):  
X.Z. Wu ◽  
Y. Yang ◽  
H.-T. Liu ◽  
Z.-Y. Yue ◽  
X.-H. Gao ◽  
...  

The objective of this study was to determine whether nutrient digestibility and growth performance of mink were affected by different dietary copper levels. Ninety-six 60-day-old healthy female minks were randomly assigned to 8 treatment groups with 12 animals in each group and fed a diet supplemented with either 0, 4, 8, 16, 32, 64, 128 or 256 mg/kg copper as CuSO<sub>4 </sub>&times; 5H<sub>2</sub>O, respectively. Our data showed that body weight and average daily gain increased (linear and quadratic, P &lt; 0.05) as Cu increased in the diet, the highest body weight and average daily gain were seen in the Cu32 group. Feed : gain ratio responded in a linear (P = 0.0025) fashion with increasing level of Cu, the lowest feed : gain ratio was seen in the Cu64 group. Digestibility of ether extract responded in a linear (P = 0.0190) fashion with increasing level of Cu. There were no differences in apparent digestibility of dry matter, CP, and gross energy among groups (P &gt; 0.05). N retention linearly (P = 0.0363) responded to increasing levels of Cu. Glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase increased (linear and quadratic, P &lt; 0.05) as Cu increased in the diet; the highest glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase were seen in the Cu256 group. Total protein of the minks was similar among the treatments, however, albumin in serum responded in a linear (P = 0.0370) and quadratic (P = 0.0049) fashion with increasing level of Cu. The activity of ceruloplasmin responded in a linear (P = 0.0001) and quadratic (P = 0.0203) fashion with increasing level of Cu. The activity of Cu-Zn superoxide dismutase responded in a linear (P = 0.0010) fashion with increasing level of Cu. Our results indicate that supplemental Cu plays an important role in the growth performance of mink, helping young female mink digest and efficiently utilize added dietary fat. &nbsp;


2019 ◽  
Vol 26 (4) ◽  
pp. 248-258
Author(s):  
Liangkang Lv ◽  
Hui Zhang ◽  
Zhengya Liu ◽  
Long Lei ◽  
Zhi Feng ◽  
...  

The present study was conducted to investigate the effects of dietary supplementation of selenium from different sources on the growth performance, nutrient digestibility, and blood immune indices of piglets orally challenged with Salmonella typhimurium (ST). In a 2 × 2 factorial arrangement, 32 piglets (6.43 ± 0.54 kg of body mass) were assigned into four groups with or without dietary inclusion of sodium selenite (SS) or yeast selenium (YS) and with or without ST challenge (5 ml 1 × 109 cfu/ml ST or 5 ml saline) on d 13. In each period, YS increased average daily feed intake and average daily gain but did not reach statistical significance. During the challenged stage, piglets fed YS had higher digestibility of dry matter, crude protein, crude fat, and YS reduced the amount of Escherichia coli in feces. Additionally, YS regulated the composition of T-lymphocyte subset and influenced the production of inflammatory cytokines. In conclusion, in this study selenium-enriched yeast was more effective in enhancing nutrient digestibility, and inhibiting inflammation and oxidative stress by inducing the activity of the lymphocytes, expression of antioxidant enzymes and so on.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qindan Dai ◽  
Jian Ma ◽  
Guang Cao ◽  
Rui Hu ◽  
Yixiao Zhu ◽  
...  

AbstractThe experiment was conducted to compare the growth performance, rumen fermentation, nutrient digestibility, and ruminal and fecal bacterial community between yaks and cattle-yaks. Ten male yaks (36-month-old) were used as the yak (YAK) group and 10 male cattle-yaks with similar age were selected as the cattle-yak (CAY) group. All the animals were fed same ration and the experiment lasted for 60 days. The results showed that the average daily gain and dry matter intake of CAY group were higher (P < 0.05) than those of YAK group. The ruminal concentrations of total volatile fatty acids, acetate, and butyrate were higher (P < 0.05) in CAY group than those in YAK group. However, the neutral detergent fiber and acid detergent fiber digestibility exhibited an opposite between two groups. In the rumen, the relative abundances of Prevotella 1 and Prevotellaceae UCG-001 were higher (P < 0.05) and Succiniclasticum and Butyrivibrio 2 were lower (P < 0.05) in YAK group compared to CAY group. In the feces, the unclassified Lachnospiraceae, Lachnospiraceae NK4A136 group, and Lachnospiraceae AC2044 group were significantly enriched (P < 0.05) in YAK group, whereas the Ruminococcaceae UCG-010, Ruminococcaceae UCG-013, and Succiniclasticum were significantly enriched (P < 0.05) in CAY group. Overall, under the same diet, the yaks have higher fiber utilization and cattle-yaks have higher energy utilization.


2021 ◽  
Vol 50 (6) ◽  
Author(s):  
M.L. Seshoka ◽  
P.J. Fourie ◽  
A.T. Kanengoni ◽  
I.M.M. Malebana ◽  
R.S. Thomas ◽  
...  

In this experiment, the effects were evaluated of various dietary inclusion levels of avocado oil cake (AOC) silage on growth performance, nutrient digestibility, and carcass characteristics in pigs. A mixture of 70% AOC, 25% wheat bran, and 5% sugarcane molasses was ensiled in 200 L drums for 90 days. It was then incorporated at 0%, 3% and 5% in the experimental diets. Twenty-seven Large White cross Landrace (LW x LR) weaner pigs, with an average live weight of 22 kg, were stratified by weight and randomly allocated to the three experimental diets (9 pigs/diet). Pigs were weighed individually each week throughout the trial. Daily feed intake, average daily gain (ADG) and feed conversion rates (FCRs) were recorded. A nutrient digestibility study was carried out on completion of the growth trial. At the end of the experiment, pigs were fasted for twelve hours and weighed to determine the final weight. The pigs were then slaughtered, and carcass samples were collected and analysed. The growth performance of pigs was not affected by diet, irrespective of fibre levels in the AOC silage. However, the addition of 3% AOC silage improved the digestibility of fibre fractions. The weights of the cold carcass, chops, and backfat thickness were reduced with AOC silage addition.


2019 ◽  
Vol 99 (4) ◽  
pp. 840-847
Author(s):  
X. Liu ◽  
Y.S. Han ◽  
I.H. Kim

The present experiment was to evaluate the effects of dietary Spirulina (SP) supplementation in growing pigs. A total of 140 pigs [(Landrace × Yorkshire) × Duroc, 25.32 ± 1.36 kg] were randomly distributed to one of four treatments: control, basal diet; treatment 1, basal diet + 0.025% SP; treatment 2, basal diet + 0.050% SP; and treatment 3, basal diet + 0.100% SP. Growing pigs fed 0.050% SP diet had greater (P < 0.05) body weight and fecal Lactobacillus counts compared with pigs fed basal diet. Average daily gain and gain to feed ratio were greater (P < 0.05) in pigs fed 0.050% and 0.100% SP diets as compared with pigs fed basal diet. The apparent total tract digestibility (ATTD) of dry matter (DM) and superoxide dismutase (SOD) activity for pigs fed 0.050% SP diet tended to increase compared with pigs fed basal diet (P < 0.10). Pigs fed 0.025%, 0.050%, and 0.100% SP had a higher (P < 0.05) glutathione peroxidase (GPx) activity than pigs fed basal diet. In conclusion, SP supplementation improved growth performance and ATTD of DM, increased the SOD and GPx activity, and enhanced the fecal Lactobacillus counts in growing pigs.


2014 ◽  
Vol 59 (No. 11) ◽  
pp. 527-535 ◽  
Author(s):  
S. Mohana Devi ◽  
IH Kim

The objective of the present study was to evaluate the effects of MCFA and probiotic (Enterococcus faecium DSM 7134) supplementation on growth performance, nutrient digestibility, blood profiles, faecal score, excreta microbiota, and excreta noxious gas emission in weanling pigs. A total of 140 weanling pigs [(Yorkshire &times; Landrace) &times; Duroc] were allotted to four treatments groups of seven replicates/treatment and five pigs/replicate. The four experimental diets included: CON diet (basal diet); T1 (CON + MCFA 0.2%); T2 (CON + probiotic 0.01%) and T3 (CON + MCFA 0.2% + probiotic 0.01%). Growth performance, average daily gain (ADG), average daily feed intake (ADFI), gain-to-feed ratio (G : F), Nutrient digestibility: dry matter (DM), nitrogen (N), energy were determined along with blood profiles: glucose, blood urea nitrogen (BUN), creatinine, high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol, triglyceride, Excreta bacteria: Lactobacillus, E. coli, Excreta gas emission: NH<sub>3</sub>, H<sub>2</sub>S, total mercaptans, acetic acid and faecal scores. Overall, average daily gain (ADG) and G : F in T2 and T3 treatment groups was higher than in T1 and controls. In blood profiles, glucose levels were found to be increased in week two and six in the treatment groups compared to controls. In addition, the nutrient digestibility of DM, N and energy were found to be increased significantly in T2 and T3 when compared to T1 and controls. There was no significant difference observed between the groups for faecal score, microflora and noxious gas emission. In conclusion, dietary MCFA and probiotic supplementation in weanling pigs are efficacious alternatives to antibiotics, and can improve health status and performance.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 179-180
Author(s):  
Jemin Ahn ◽  
Xiang Ao ◽  
Haoyang Sun ◽  
In Ho Kim

Abstract This study was conducted to evaluate the effects of carbohydrases on growth performance, nutrient digestibility, blood profiles and fecal microbiota in weanling pigs fed reduced energy diet. A total of 160 cross-bred [(Yorkshire × Landrace) × Duroc)] weanling pigs with an initial BW of 7.23 ± 1.08 kg were used in this 42-day trial. Pigs were randomly allocated in 1 of the following 4 dietary treatments: (1) PC, positive diet (NE 2,545 kcal/kg); (2) NC, negative diet (NE 2470 kcal/kg); (3) NC1, NC + 0.1% carbohydrases; (4) NC2, NC + 0.2% carbohydrases based on the sex and BW. There were 8 replications (pens) per treatment with 5 pigs per pen. During d 0-7, pigs fed NC diet had lower (P < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) than those fed PC and NC2 diets. NC group reduced (P < 0.05) ADG throughout the experiment compared with PC and NC2 groups. NC group reduced (P < 0.05) the digestibility of dry matter, nitrogen and gross energy. Taken together, reduced energy diet caused detrimental effects on growth performance and nutrient digestibility in weanlings, while the addition of carbohydrases (0.2%) can partially counteract these negative effects.


2009 ◽  
Vol 89 (1) ◽  
pp. 85-89 ◽  
Author(s):  
R T Zijlstra ◽  
K. Menjivar ◽  
E. Lawrence ◽  
E. Beltranena

The effects of substituting wheat with crude glycerol as a dietary energy source were evaluated. Seventy-two weaned pigs were fed for 4 wk one of three pelleted wheat-based diets containing 0, 4, or 8% glycerol and formulated to 2.28 Mcal kg-1 net energy (NE) and 5.02 g standardized ileal digestible lysine Mcal-1 NE. For day 0 to 28, body weight increased linearly (P = 0.04); pigs fed 8% glycerol were 1.11 kg heavier than pigs fed 0% glycerol. Glycerol inclusion tended to increase average daily gain linearly (P = 0.066) and increased average daily feed intake quadratically (P = 0.037) without affecting feed efficiency (P > 0.10). Feeding up to 8% dietary crude glycerol by substituting wheat can enhance the growth performance of weaned pigs. Key words: Co-product, digestibility, glycerol, weaned pig


2019 ◽  
Vol 157 (5) ◽  
pp. 456-468
Author(s):  
V. N. Gouvêa ◽  
M. A. P. Meschiatti ◽  
J. M. M. Moraes ◽  
C. D. A. Batalha ◽  
J. R. R. Dórea ◽  
...  

AbstractThe current study evaluated growth performance and digestion responses of finishing bulls fed diets containing 825 g/kg flint maize [dry matter (DM) basis] ground to medium (1.66 mm; MG) or coarse particle sizes (2.12 mm; CG), with added monensin (26 mg/kg; DM basis; MON) or a blend of essential oils (BEO) + exogenous α-amylase (AM; 90 mg/kg + 560 mg/kg commercial product, respectively, DM basis). In Expt 1, 256 Nellore bulls were blocked by initial body weight (BW) (360 ± 11.7 kg) and assigned to 48 pens in a 2 × 2 factorial arrangement of treatments. Effect of a maize particle size × feed additive interaction was not detected for final BW, DM intake (DMI), average daily gain (ADG) and feed efficiency. The DMI was greater for bulls fed BEO + AM v. MON. Final BW and ADG tended to be greater for bulls fed CG than MG maize. An interaction was detected for hot carcass weight which was 11 kg heavier for bulls fed BEO + AM v. MON in diets containing CG, but not MG particle size. In Expt 2, four ruminally cannulated Nellore steers were offered the same treatments as Expt 1, in a 4 × 4 Latin Square design. Intake of most nutrients was greater for steers fed CG than steers fed MG maize. In summary, feeding bulls CG maize increased growth performance and carcass characteristics compared with MG. The combination of BEO + AM resulted in heavier carcass weights compared with MON supplementation when included in diets containing CG maize.


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