scholarly journals Proteomic Analysis of the Protective Effect of Early Heat Exposure against Chronic Heat Stress in Broilers

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2365
Author(s):  
Da Rae Kang ◽  
Kwan Seob Shim
Keyword(s):  
Global Warming ◽  
Heat Stress ◽  
Heat Exposure ◽  
Proteome Analysis ◽  
Differentially Expressed ◽  
Poultry Production ◽  
Differentially Expressed Proteins ◽  
Physiological Mechanisms ◽  
Stress Effects ◽  
Increasing Trend

The increasing trend of global warming has affected the livestock industry through the heat stress, especially in poultry. Therefore, a better understanding of the mechanisms of heat stress in poultry would be helpful for maintaining the poultry production. Three groups were designed to determine early heat stress effects during chronic heat stress: CC, raised at a comfortable temperature; CH, chronic heat exposure at 35 °C for 21–35 days continuously; and HH, early heat exposure at 40 °C for 24 h at 5 days old with 35 °C temperature for 21–35 days continuously. In this study, proteome analysis was carried out to identify differentially expressed proteins in the liver tissue of broilers under chronic and early heat exposure. There were eight differentially expressed proteins from early heat stress during chronic heat exposure, which were related to actin metabolism. According to KEGG (Kyoto encyclopedia of genes and genomes) analysis, the proteins involved in carbohydrate metabolism were expressed to promote the metabolism of carbohydrates under chronic heat stress. Early heat reduced the heat stress-induced expression changes of select proteins. Our study has shown that early heat exposure suggests that the liver of broilers has various physiological mechanisms for regulating homeostasis to aid heat resistance.

scholarly journals Proteomic Analysis of the Protective Effect of Early Heat Exposure Against Chronic Heat Stress in Broilers

2020 ◽  
Author(s):  
Darae Kang ◽  
Kwan Seob Shim
Keyword(s):  
Heat Stress ◽  
Carbohydrate Metabolism ◽  
Cell Damage ◽  
Heat Exposure ◽  
Proteome Analysis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Thermal Reactions ◽  
Liver Proteome

Abstract BackgroundThe increasing trend of global warming has affected the livestock industry through the heat stress caused to the animals. Among them, poultry are the most susceptible to heat stress, which results in serious production problems. Therefore, a better understanding of the mechanisms related to the thermal reactions and thermal resistance in poultry would be helpful toward resolving the production issues. In this study, whole proteome analysis was carried out to identify differentially expressed proteins in the liver tissue of broilers under chronic heat stress (35℃/24 h, each day between the broiler ages of 21–35 days). Additionally, the effect of early heat exposure (40℃/24 h, for 1 day only on chicks at 5 days of age) was determined.ResultsIn total, 277 differentially expressed proteins due to chronic heat stress were identified (132 downregulated and 145 upregulated). Of those, 95 proteins were regulated by early heat exposure (42 downregulated and 53 upregulated during chronic heat stress). Of the 95 proteins, 8 were related to actin metabolism. According to the KEGG analysis, the proteins were mainly involved in pathways for carbon metabolism and carbohydrate metabolism. Under chronic heat stress, the proteins involved in carbohydrate metabolism were expressed in such a way as to promote the metabolism of carbohydrates, which is the natural means to reduce body temperature but may well induce cell damage.ConclusionEarly heat exposure reduced the heat stress-induced expression changes of select proteins, indicating the adaptability of the animal to chronic heat stress. The determination of the differentially expressed proteins in the liver proteome under chronic heat stress and early heat exposure suggests that the liver of broilers has various physiological mechanisms for regulating homeostasis to aid heat resistance.

scholarly journals HEAT STRESS IN POULTRY INDUSTRY

2016 ◽  
Vol 8 (2) ◽  
pp. 87-101 ◽  
Author(s):  
Miloš Kapetanov ◽  
Marko Pajić ◽  
Dragana Ljubojević ◽  
Miloš Pelić
Keyword(s):  
Global Warming ◽  
Heat Stress ◽  
Preventive Measures ◽  
Production Performance ◽  
Poultry Production ◽  
Summer Period ◽  
Temperature Oscillations ◽  
Increasing Trend ◽  
The Republic ◽  
Continental Climate

Th e results of our 15-year long research on the eff ects of global warming in our region that relies on the database obtained from the Republic Hydrometeorological Service of the Republic of Serbia strongly suggested changes in the pattern of disease events associated with inevitable occurrence of heat stress in poultry regardless of the species and category. Having in mind the accelerating global warming and geographic position of Serbia the occurrence of frequent cyclical and intense meteorological extremes is expected (Kapetanov et al., 2011; Kapetanov et al., 2013; Kapetanov et al., 2013). In our country and surrounding regions characterized by continental climate, the risk from the heat stress in poultry production is limited to summer months. Still, summer period has been getting longer and is associated with increasing trend of tropical days and annual trend of global warming spreading of 0.2o geographic latitude. Heat stress of poultry is one of the most challenging problems of poultry production causing substantial damages and aff ecting all parameters of production performance and is oft en associated with sudden and massive deaths. Having in mind the aforementioned data, the objective of our research was monitoring of changes in mortality structure associated with temperature oscillations during production process with an aim of emphasizing some major sources, consequences and preventive measures related to heat stress.

scholarly journals Investigation of multiple sclerosis-related pathways through the integration of genomic and proteomic data

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11922
Author(s):  
Elif Everest ◽  
Ege Ülgen ◽  
Ugur Uygunoglu ◽  
Melih Tutuncu ◽  
Sabahattin Saip ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
False Negative ◽  
Proteome Analysis ◽  
Functional Enrichment ◽  
Differentially Expressed ◽  
Coagulation Cascade ◽  
Differentially Expressed Proteins ◽  
Proteomic Data ◽  
Genome Wide ◽  
Immunological Mechanisms

Background Multiple sclerosis (MS) has a complex pathophysiology, variable clinical presentation, and unpredictable prognosis; understanding the underlying mechanisms requires combinatorial approaches that warrant the integration of diverse molecular omics data. Methods Here, we combined genomic and proteomic data of the same individuals among a Turkish MS patient group to search for biologically important networks. We previously identified differentially-expressed proteins by cerebrospinal fluid proteome analysis of 179 MS patients and 42 non-MS controls. Among this study group, 11 unrelated MS patients and 60 independent, healthy controls were subjected to whole-genome SNP genotyping, and genome-wide associations were assessed. Pathway enrichment analyses of MS-associated SNPs and differentially-expressed proteins were conducted using the functional enrichment tool, PANOGA. Results Nine shared pathways were detected between the genomic and proteomic datasets after merging and clustering the enriched pathways. Complement and coagulation cascade was the most significantly associated pathway (hsa04610, P = 6.96 × 10−30). Other pathways involved in neurological or immunological mechanisms included adherens junctions (hsa04520, P = 6.64 × 10−25), pathogenic Escherichia coli infection (hsa05130, P = 9.03 × 10−14), prion diseases (hsa05020, P = 5.13 × 10−13). Conclusion We conclude that integrating multiple datasets of the same patients helps reducing false negative and positive results of genome-wide SNP associations and highlights the most prominent cellular players among the complex pathophysiological mechanisms.

Proteome analysis of virulent Aeromonas hydrophila reveals the upregulation of iron acquisition systems in the presence of a xenosiderophore

2020 ◽  
Vol 367 (20) ◽  
Author(s):  
Miles D Lange ◽  
Jason Abernathy ◽  
Craig A Shoemaker ◽  
Dunhua Zhang ◽  
Augustus Kirby ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Ictalurus Punctatus ◽  
Aeromonas Hydrophila ◽  
Iron Acquisition ◽  
Chelating Agent ◽  
Proteome Analysis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Protein Protein Interactions ◽  
Virulent Aeromonas Hydrophila

ABSTRACT The Gram-negative bacterium, Aeromonas hydrophila, has been responsible for extensive losses in the catfish industry for over a decade. Due to this impact, there are ongoing efforts to understand the basic mechanisms that contribute to virulent A. hydrophila (vAh) outbreaks. Recent challenge models demonstrated that vAh cultured in the presence of the iron chelating agent deferoxamine mesylate (DFO) were more virulent to channel catfish (Ictalurus punctatus). Interestingly, differential gene expression of select iron acquisition genes was unremarkable between DFO and non-DFO cultures, posing the question: why the increased virulence? The current work sought to evaluate growth characteristics and protein expression of vAh after the addition of DFO. A comparative proteome analysis revealed differentially expressed proteins among tryptic soy broth (TSB) and TSB + DFO treatments. Upregulated proteins identified among the TSB + DFO treatment were enriched for gene ontology groups including iron ion transport, siderophore transport and siderophore uptake transport, all iron acquisition pathways. Protein-protein interactions were also evaluated among the differentially expressed proteins and predicted that many of the upregulated iron acquisition proteins likely form functional physiological networks. The proteome analysis of the vAh reveals valuable information about the basic biological processes likely leading to increased virulence during iron restriction in this organism.

scholarly journals Proteomic Study of Hypothalamus in Pigs Exposed to Heat Stress

2020 ◽  
Author(s):  
Tianyue Yu ◽  
Yan-Hong Yong ◽  
Jun-yu Li ◽  
Biao Fang ◽  
Can-ying Hu ◽  
...  
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Regulatory Networks ◽  
Protein Profile ◽  
Absolute Quantification ◽  
The Body ◽  
Differentially Expressed ◽  
Farm Animals ◽  
Differentially Expressed Proteins ◽  
The Brain

Abstract Background : With evidence of warming climates, it is important to understand the effects of heat stress in farm animals in order to minimize production losses. Studying the changes in the brain proteome induced by heat stress may aid in understanding how heat stress affects brain function. The hypothalamus is a critical region in the brain that controls the pituitary gland, which is responsible for the secretion of several important hormones. In this study, we examined the hypothalamic protein profile of 10 pigs (15 ± 1 kg body weight), with five subjected to heat stress (35 ± 1 °C; relative humidity = 90%) and five acting as controls (28 ± 3°C; RH = 90%). Result: The isobaric tags for relative and absolute quantification (iTRAQ) analysis of the hypothalamus identified 1710 peptides corresponding to 360 proteins, including 295 differentially expressed proteins (DEPs), 148 of which were up-regulated and 147 down-regulated, in heat-stressed animals. The Ingenuity Pathway Analysis (IPA) software predicted 30 canonical pathways, four functional groups, and four regulatory networks of interest. The DEPs were mainly concentrated in the cytoskeleton of the pig hypothalamus during heat stress. Conclusions: In this study, heat stress significantly increased the body temperature and reduced daily gain of body weight in pigs. Furthermore, we identified 295 differentially expressed proteins, 147 of which were down-regulated and 148 up-regulated in hypothalamus of heat stressed pigs. The IPA showed that the DEPs identified in the study are involved in cell death and survival, cellular assembly and organization, and cellular function and maintenance, in relation to neurological disease, metabolic disease, immunological disease, inflammatory disease, and inflammatory response. We hypothesize that a malfunction of the hypothalamus may destroy the host physical and immune function, resulting in decreased growth performance and immunosuppression in heat stressed pigs.

scholarly journals Response of Endozoicomonas montiporae to heat stress and coral host lysates

2021 ◽  
Author(s):  
YaFan Chan ◽  
Chia-Yu Chen ◽  
Chih-Ying Lu ◽  
Yung-Chi Tu ◽  
Kshitij Tandon ◽  
...  
Keyword(s):  
Heat Stress ◽  
Antioxidant Defense ◽  
Molecular Mechanisms ◽  
Proteome Analysis ◽  
Differentially Expressed ◽  
Coral Host ◽  
Defense Proteins ◽  
Depth Analysis ◽  
Pyruvate Synthase ◽  
Shock Proteins

Endozoicomonas, a core bacterial group in corals, may also be a coral symbiont. Endozoicomonas communities often decrease rapidly in corals under heat stress. However, how the bacteria respond to changes in temperature and coral host during heat stress is unknown. Here, we employed the cultivable, dominant species E. montiporae as a working organism to explore how Endozoicomonas responds to heat stress. We designed two experiments to clarify the extent to which E. montiporae is influenced by temperature and coral host. We detected differentially expressed protein (DEP) profiles in this bacterium at 31°C and 33°C compared to 25°C by tandem mass tags-based quantitative proteome analysis. Fifty DEPs, including many heat shock proteins, were detected when the temperature changed. The expression of antioxidant defense proteins and key pyruvate synthase proteins decreased, suggesting that E. montiporae were in a physiology of stress at 33°C. Furthermore, some proteins were differentially expressed because of the heat-stress-treated coral lysate specifically, suggesting that not only heat but also heat-induced host factors can affect the protein expression of the bacterium. This study provides an in-depth analysis of how the molecular mechanisms of Endozoicomonas are affected by heat stress and coral host.

scholarly journals Differential Proteome Analysis of Hybrid Bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) Under Fungal Stress (Arthrinium phaeospermum)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shujiang Li ◽  
Xinmei Fang ◽  
Shan Han ◽  
Tianhui Zhu ◽  
Hanmingyue Zhu
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Proteome Analysis ◽  
Enrichment Analysis ◽  
Pathogenic Fungi ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Differential Proteins ◽  
Arthrinium Phaeospermum

AbstractIn this study, TMT (tandem mass tag)-labeled quantitative protein technology combined with LC–MS/MS (liquid chromatography-mass spectrometry/mass spectrometry) was used to isolate and identify the proteins of the hybrid bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) and the bamboo inoculated with the pathogenic fungi Arthrinium phaeospermum. A total of 3320 unique peptide fragments were identified after inoculation with either A. phaeospermum or sterile water, and 1791 proteins were quantified. A total of 102 differentially expressed proteins were obtained, of which 66 differential proteins were upregulated and 36 downregulated in the treatment group. Annotation and enrichment analysis of these peptides and proteins using the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases with bioinformatics software showed that the differentially expressed protein functional annotation items were mainly concentrated on biological processes and cell components. The LC–PRM/MS (liquid chromatography-parallel reaction monitoring/mass spectrometry) quantitative analysis technique was used to quantitatively analyze 11 differential candidate proteins obtained by TMT combined with LC–MS/MS. The up–down trend of 10 differential proteins in the PRM results was consistent with that of the TMT quantitative analysis. The coincidence rate of the two results was 91%, which confirmed the reliability of the proteomic results. Therefore, the differentially expressed proteins and signaling pathways discovered here may be the further concern for the bamboo-pathogen interaction studies.

scholarly journals Proteomic changes of the porcine small intestine in response to chronic heat stress

2015 ◽  
Vol 55 (3) ◽  
pp. 277-293 ◽  
Author(s):  
Yanjun Cui ◽  
Xianhong Gu
Keyword(s):  
Heat Stress ◽  
Tricarboxylic Acid Cycle ◽  
Cell Structure ◽  
Heat Exposure ◽  
Proteome Analysis ◽  
Tca Cycle ◽  
Intestinal Morphology ◽  
Negative Effects ◽  
Free Triiodothyronine ◽  
Intestinal Integrity

Acute heat stress (HS) negatively affects intestinal integrity and barrier function. In contrast, chronic mild HS poses a distinct challenge to animals. Therefore, this study integrates biochemical, histological and proteomic approaches to investigate the effects of chronic HS on the intestine in finishing pigs. Castrated male crossbreeds (79.00±1.50 kg BW) were subjected to either thermal neutral (TN, 21 °C; 55%±5% humidity; n=8) or HS conditions (30 °C; 55%±5% humidity; n=8) for 3 weeks. The pigs were sacrificed after 3 weeks of high environmental exposure and the plasma hormones, the intestinal morphology, integrity, and protein profiles of the jejunum mucosa were determined. Chronic HS reduced the free triiodothyronine (FT3) and GH levels. HS damaged intestinal morphology, increased plasma d-lactate concentrations and decreased alkaline phosphatase activity of intestinal mucosa. Proteome analysis of the jejunum mucosa was conducted by 2D gel electrophoresis and mass spectrometry. Fifty-three intestinal proteins were found to be differentially abundant, 18 of which were related to cell structure and motility, and their changes in abundance could comprise intestinal integrity and function. The down-regulation of proteins involved in tricarboxylic acid cycle (TCA cycle), electron transport chain (ETC), and oxidative phosphorylation suggested that chronic HS impaired energy metabolism and thus induced oxidative stress. Moreover, the changes of ten proteins in abundance related to stress response and defense indicated pigs mediated long-term heat exposure and counteracted its negative effects of heat exposure. These findings have important implications for understanding the effect of chronic HS on intestines.

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