scholarly journals The Serum and Saliva Proteome of Dogs with Diabetes Mellitus

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2261
Author(s):  
Lorena Franco-Martínez ◽  
Andrea Gelemanović ◽  
Anita Horvatić ◽  
María Dolores Contreras-Aguilar ◽  
Vladimir Mrljak ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Mellitus Type 1 ◽  
Healthy Controls ◽  
Serum Samples ◽  
Proteomic Data ◽  
Proteomic Approach ◽  
Paired Serum ◽  
Canine Diabetes Mellitus ◽  
Type 1 Dm ◽  
First Time

This study aims to evaluate the changes in salivary and serum proteomes that occur in canine diabetes mellitus type-1 (DM) through a high-throughput quantitative proteomic analysis. The proteomes of 10 paired serum and saliva samples from healthy controls (HC group, n = 5) and dogs with untreated DM (DM group, n = 5) were analyzed using Tandem Mass Tags (TMT)-based proteomic approach. Additionally, 24 serum samples from healthy controls and untreated DM were used to validate haptoglobin in serum. The TMT analysis quantified 767 and 389 proteins in saliva and serum, respectively. Of those, 16 unique proteins in serum and 26 in saliva were differently represented between DM and HC groups. The verification of haptoglobin in serum was in concordance with the proteomic data. Our results pointed out changes in both saliva and serum proteomes that reflect different physiopathological changes in dogs with DM. Although some of the proteins identified here, such as malate dehydrogenase or glyceraldehyde-3-phosphate dehydrogenase, were previously related with DM in dogs, most of the proteins modulated in serum and saliva are described in canine DM for the first time and could be a source of potential biomarkers of the disease. Additionally, the molecular function, biological process, pathways and protein class of the differential proteins were revealed, which could improve the understanding of the disease’s pathological mechanisms.

Simultaneous Juvenile Idiopathic Arthritis and Diabetes Mellitus Type 1 — A Finnish Nationwide Study

2011 ◽  
Vol 39 (2) ◽  
pp. 377-381 ◽  
Author(s):  
HEINI POHJANKOSKI ◽  
HANNU KAUTIAINEN ◽  
MATTI KORPPI ◽  
ANNELI SAVOLAINEN
Keyword(s):  
Diabetes Mellitus ◽  
Juvenile Idiopathic Arthritis ◽  
Autoimmune Disease ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Laboratory Findings ◽  
Type 1 Dm ◽  
Psychiatric Problems ◽  
Rheumatoid Factor Seropositivity

Objective.To describe the occurrence and main clinical and laboratory findings of patients having both juvenile idiopathic arthritis (JIA) and diabetes mellitus type 1 (DM-1) in a period of 30 years.Methods.Eighty-two patients having simultaneous JIA and DM-1 were identified in the reimbursement registers of the Finnish National Institute of Insurance during the period 1976–2005. Data on their clinical histories were collected from patient files.Results.Occurrence of simultaneous JIA and DM-1 increased 4.5-fold between the first (1976-85) and the last (1996–2005) decade. Prevalence of uveitis was 7%, of rheumatoid factor seropositivity 15%; 22% of patients had a third autoimmune disease [autoimmune disease (AID)], and 16% had serious psychiatric problems.Conclusion.The occurrence of patients with the 2 diseases, JIA and DM-1, increased over 3 decades. The prevalence of uveitis was low, the number of seropositive patients was high, and further cases of AID were frequent. Patients had multiple additional problems necessitating multiprofessional care.

scholarly journals Evaluation of Vascular Endothelial Function in Children with Type 1 Diabetes Mellitus

2021 ◽  
Vol 10 (21) ◽  
pp. 5065
Author(s):  
Karolina Nocuń-Wasilewska ◽  
Danuta Zwolińska ◽  
Agnieszka Zubkiewicz-Kucharska ◽  
Dorota Polak-Jonkisz
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Endothelial Dysfunction ◽  
Type 1 Diabetes Mellitus ◽  
Diabetes Mellitus Type 1 ◽  
Vascular Endothelial ◽  
Healthy Controls ◽  
Linear Correlations ◽  
Pai 1

Diabetic kidney disease belongs to the major complications of diabetes mellitus. Here, hyperglycaemia is a key metabolic factor that causes endothelial dysfunction and vascular changes within the renal glomerulus. The aim of the present study was to assess the function of the vascular endothelium in children with type 1 diabetes mellitus (type 1 diabetes) by measuring selected endothelial lesion markers in blood serum. The selected markers of endothelial lesions (sVCAM-1, sICAM-1, sE-SELECTIN, PAI-1, ADMA and RAGE) were assayed by the immunoenzymatic ELISA method. The study involved 66 patients (age: 5–18 years) with type 1 diabetes and 21 healthy controls (age: 5–16 years). In the type 1 diabetes patients, significantly higher concentrations of all of the assayed markers were observed compared to the healthy controls (p < 0.001). All of the evaluated markers positively correlated with the disease duration, the age, and BMI of the patients, while only PAI-1 and sE-SELECTIN were characteristic of linear correlations with the estimated glomerular filtration rate (eGFR). It can be concluded that endothelial inflammatory disease occurs in the early stages of type 1 diabetes mellitus in children. The correlations between PAI-1, sE-SELECTIN, and eGFR suggest an advantage of these markers over other markers of endothelial dysfunction as prognostic factors for kidney dysfunction in children with type 1 diabetes.

scholarly journals Serum Metabolites as an Indicator of Developing Gestational Diabetes Mellitus Later in the Pregnancy: A Prospective Cohort of a Chinese Population

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Mengyuan Tian ◽  
Shujuan Ma ◽  
Yiping You ◽  
Sisi Long ◽  
Jiayue Zhang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Early Pregnancy ◽  
Pantothenic Acid ◽  
Maternal Serum ◽  
Control Group ◽  
Healthy Controls ◽  
Serum Samples ◽  
Serum Metabolites

Objective. Gestational diabetes mellitus (GDM) is a common metabolic disorder with onset during pregnancy. However, the etiology and pathogenesis of GDM have not been fully elucidated. In this study, we used a metabolomics approach to investigate the relationship between maternal serum metabolites and GDM in early pregnancy. Methods. A nested case-control study was performed. To establish an early pregnancy cohort, pregnant women in early pregnancy ( 10 ‐ 13 + 6 weeks) were recruited. In total, 51 patients with GDM and 51 healthy controls were included. Serum samples were analyzed using an untargeted high-performance liquid chromatography mass spectrometry metabolomics approach. The relationships between metabolites and GDM were analyzed by an orthogonal partial least-squares discriminant analysis. Differential metabolites were evaluated using a KEGG pathway analysis. Results. A total of 44 differential metabolites were identified between GDM cases and healthy controls during early pregnancy. Of these, 26 significant metabolites were obtained in early pregnancy after false discovery rate ( FDR < 0.1 ) correction. In the GDM group, the levels of L-pyroglutamic acid, L-glutamic acid, phenylacetic acid, pantothenic acid, and xanthine were significantly higher and the levels of 1,5-anhydro-D-glucitol, calcitriol, and 4-oxoproline were significantly lower than those in the control group. These metabolites were involved in multiple metabolic pathways, including those for amino acid, carbohydrate, lipid, energy, nucleotide, cofactor, and vitamin metabolism. Conclusions. We identified significant differentially expressed metabolites associated with the risk of GDM, providing insight into the mechanisms underlying GDM in early pregnancy and candidate predictive markers.

scholarly journals Support System Keluarga dalam Pencegahan Ketoasidosis Diabetik pada Anak dengan DM Tipe 1

2019 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Ana Fitria Nusantara ◽  
Sunanto Sunanto ◽  
Achmad Kusyairi
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Family Support ◽  
Support System ◽  
Parental Support ◽  
Diabetes Mellitus Type 1 ◽  
Qualitative Research Design ◽  
Life Threatening ◽  
Type 1 Dm

AbstrakKetoasidosis diabetik (KAD) merupakan komplikasi akut diabetes melitus tipe 1 yang ditandai dengan dehidrasi, kehilangan elektrolit, asidosis dan disebabkan oleh pembentukan keton  yang  berlebihan.  Keadaan ini merupakan gangguan metabolisme yang paling serius dan mengancam jiwa. Namun demikian kejadian KAD dapat dicegah dengan beberapa cara, salah satunya adalah dengan menejemen diabetik yang benar. Penelitian bertujuan mengidentifikasidukungan keluarga dalam mencegah ketoasidosis diabetik.Desain penelitian kualitatif digunakan pada penelitian ini dengan pendekatan phenomenology. Data dikumpulkan dengan tehnik wawancara tidak terstruktur kemudian dianalisis dengan tehnik Van Manen.Hasil penelitian menunjukkan  bahwa support sistem keluarga dapat dilakukan dengan cara membawakan bekal makan atau minum untuk kegiatan di luar rumah, mengkomunikasikan penyakit anak kepada pihak lain di luar rumah (sekolah, tetangga, saudara), melakukan injeksi insulin, pengawasan diet, serta kunjungan rutin ke rumah sakit. Anak dengan DM tipe 1 berada pada usia yang sangat muda sehingga dalam hal perawatan dan penatalaksanaan diabetesnya sangat tergantung pada orang tua selaku penanggung jawab dalam perawatan anak. Dukungan orang tua dalam perawatan anak dengan DM tipe 1 sangat berdampak pada pencegahan terjadinya komplikasi seperti ketoasidosis diabetik.Kata kunci: support system, ketoasidosis diabetik, diabetes mellitus tipe 1  AbstractDiabetic ketoacidosis (DKA) is an acute complication of type 1 diabetes mellitus which is characterized by dehydration, loss of electrolytes, acidosis and is caused by excessive ketone formation. This situation is the most serious and life-threatening metabolic disorder. However, the incidence of DKA can be prevented in several ways, one of them is correctly diabetic management. The study aims to identify family support in preventing diabetic ketoacidosis. A qualitative research design was used in this study with a phenomenology approach. Data was collected using unstructured interview techniques and then analyzed with Van Manen techniques. The results showed that family support system are by bringing food or drink supplies to the activities outside the home, communicating children's illnesses to other parties outside the home (school, neighbors, relatives), injecting insulin, supervising diet, and regular visits to hospital. Children with type 1 diabetes are at a very young age so that in case of diabetes care and management aredepent on parents as the responsible person in child care. Parental support to the children with type 1 DM are greatly affects to the prevention of complications such as diabetic ketoacidosis.Keywords: support system, ketoacidosis diabetic, diabetes mellitus type 1

scholarly journals Glycemic control in children and young adults with different types of diabetes mellitus in Ukraine

2021 ◽  
pp. 7-14
Author(s):  
E. V. Globa ◽  
N. B. Zelinska
Keyword(s):  
Diabetes Mellitus ◽  
Glycemic Control ◽  
Monogenic Diabetes ◽  
Different Types ◽  
Pathogenetic Therapy ◽  
Children And Young Adults ◽  
Type 1 Dm ◽  
First Time

Introduction. Until recently, glycated hemoglobin (HbA1c) has not been studied in children with different types of diabetes mellitus (DM). With the development of molecular genetics, new types of diabetes have been diagnosed, including neonatal (ND) and maturity onset diabetes of the young (MODY), which usually require specific pathogenetic therapy, including sulfonylureas (SU), which improve glycemic control in patients with certain mutations. The aim of this study, which was conducted for the first time in Ukraine, was to analyze glycemic control in patients with different types of DM, including ND and MODY and changing their treatment after genetic testing. Materials and methods. We studied conducted the analysis of glycemic control in patients depending on the type of diabetes, namely: type 1 diabetes (n = 9282), type 2 diabetes (n = 23), ND (n = 16) with mutations KCNJ11, ABCC8 and MODY (n = 16) with mutations in HNF1A/HNF4A and ABCC8 genes. To confirm the nature of monogenic diabetes, targeting next-generation sequencing (tNGS) of all known neonatal and monogenic diabetes genes was performed. Results. According to the Register in 2018, in Ukraine the average age of children with type 1 DM was 11.5 ± 1.4 years, and the level of HbA1c was unsatisfactory (8.8 ± 2.01 %). The proportion of children who had ideal or optimal glycemic control (HbA1c

Altered Serum Oxytocinase and Enkephalin-Degrading Aminopeptidase Activities in Patients With Fibromyalgia

2019 ◽  
Vol 21 (4) ◽  
pp. 431-439 ◽  
Author(s):  
José Manuel Martínez-Martos ◽  
María Correa-Rodríguez ◽  
Alma Rus ◽  
Francisco Molina ◽  
María Jesús Ramírez-Expósito ◽  
...  
Keyword(s):  
Roc Analysis ◽  
Activity Levels ◽  
Healthy Controls ◽  
Serum Samples ◽  
Chronic Pain Condition ◽  
Analgesic Effects ◽  
Unclear Etiology ◽  
First Time ◽  
Control Study

Objectives: Fibromyalgia (FM) is a chronic pain condition of unclear etiology. We have analyzed, for the first time, the activity of a broad spectrum of aminopeptidases (APs) in patients with FM and controls to investigate whether they are involved in the pathophysiology of this syndrome. Method: In this case–control study, we fluorometrically measured specific AP activities in serum samples of 75 patients with FM and 29 healthy controls. The predictive value of AP activities in FM was determined by receiver operating characteristic (ROC) analysis. Results: Oxytocinase activity was higher in patients with FM than in controls ( p < .001). A subgroup of patients with FM ( n = 18; 24%) showed low levels of enkephalin-degrading aminopeptidase (EDA) activity when compared with the healthy controls ( p < .001) and with the rest of FM patients ( p < .001). There were no significant differences in the activity levels of aminopeptidase A, aminopeptidase B, aspartyl aminopeptidase, insulin-regulated aminopeptidase, pyroglutamyl aminopeptidase, or aminopeptidase N between FM patients and controls. According to ROC analysis, oxytocinase activity may be a good marker for differentiating individuals with FM from healthy subjects. Conclusions: Our findings show that serum oxytocinase activity is increased in patients with FM, which could alter the metabolism of peptides with analgesic effects such as oxytocin and enkephalins. The determination of serum oxytocinase activity may aid in FM diagnosis. Additionally, we have identified a subpopulation of FM patients with abnormally low serum EDA activity.

scholarly journals A COMPARATIVE STUDY OF SERUM ASCORBATE BETWEEN NEWLY DIAGNOSED TYPE 2 DIABETICS AND LONG STANDING TYPE 2 DIABETICS ON TREATMENT

2021 ◽  
Vol 9 (08) ◽  
pp. 369-372
Author(s):  
Abdur Rahman ◽  
◽  
Usha Rani Pegu ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Vitamin C ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Newly Diagnosed ◽  
Serum Samples ◽  
Medical College ◽  
Type 2 Diabetics ◽  
Oral Hypoglycemic Drugs

Introduction: diabetes mellitus is a metabolic disorder prevalent worldwide . Oxidative stress has been the Cause of complications which arises gradually due to prolonged hyperglycemia. Vitamin c being a potent antioxidant is well consumed in diabetes mellitus. Methods: Serum samples of diabetes mellitus type 2 patients from jorhat medical college of Assam were tested to estimate ascorbate levels using spectrophotometer. Whole blood samples were tested to estimate glycated hemoglobin levels in d-10 biorad hplc machine. A total of 58 patients and equal numbers of age and sex matched apparently healthy controls were included. Results: significantly lower levels of ascorbate were recorded in diabetic cases compared to controls. Newly diagnosed cases had lesser serum ascorbate levels compared to patients who were on treatment with oral hypoglycemic drugs and insulin. Conclusion: the study was done to look for any alteration in serum ascorbic acid levels in diabetes mellitus patients. Patients newly diagnosed had lesser vitamin c levels compared to patients already on medications. It is found that vitamin c is significantly lower in diabetic patients with or without medications compared to healthy controls.

scholarly journals Assessment of the functional state of the microvasculature in children with diabetes mellitus type 1

2020 ◽  
Vol 11 (2) ◽  
pp. 71-80
Author(s):  
N. V. Malyuzhinskaya ◽  
K. V. Stepanenko ◽  
E. I. Volchansky
Keyword(s):  
Diabetes Mellitus ◽  
Functional State ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Control Group ◽  
Healthy Children ◽  
Regulation Mechanisms ◽  
Glycated Hemoglobin Level ◽  
Type 1 Dm

Objective: to assess the functional state of the microvasculature in children with diabetes mellitus type 1 (DM type 1).Materials and methods: 63 children with a verifi ed diagnosis of diabetes mellitus type 1 were examined. Th e control group consisted of 30 practically healthy children. Methods: clinical, paraclinical (determination of glycated hemoglobin level, study of microcirculation indicators using laser Doppler fl owmetry (LDF), statistical.Results: microcirculatory disorders accompanying the course of diabetes mellitus type 1 depending on the length of illness were identifi ed. In children with diabetes mellitus type 1 with standing less than 3 years an increase in the average modulation of blood fl ow mainly due to passive regulation mechanisms and the predominance of hypera adaptation in assessing the functional states of microcirculation of varying severity with an increase in the energy of oscillatory processes were observed. Signs of non-nutritive hyperemia in the zone rich in arteriovenous anastamoses and a decrease in perfusion due to an increase in perfusion fl uctuations and coeffi cient of variation in the distal extremities, as well as a decrease in amplitudes in the active tone-forming range, a gradual decrease in the energy of oscillations and randomness criteria were diagnosed with standing in the duration of the disease.Conclusions: disorders in children with diabetes mellitus type 1 microcirculatory detected using LDF are staged. Th e contribution of non-nutritive blood fl ow to microcirculation increases as the disease progresses, which leads to tissue hypoxia. Evaluation of the combination of energy, information and non-linear parameters of the oscillatory component of the blood flow allows you to identify the type of functional state in the microcirculation system.

scholarly journals Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 741
Author(s):  
Lorena Franco-Martínez ◽  
Andrea Gelemanović ◽  
Anita Horvatić ◽  
María Dolores Contreras-Aguilar ◽  
Roman Dąbrowski ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Mammary Tumors ◽  
Human Breast ◽  
Heavy Chains ◽  
Proteomic Data ◽  
Canine Mammary Tumors ◽  
Paired Serum ◽  
Immunoglobulin Gamma ◽  
First Time ◽  
Sample Set

The aim of this study was to evaluate changes in serum and saliva proteomes in canine mammary tumors (CMT) using a high-throughput quantitative proteomic analysis in order to potentially discover possible biomarkers of this disease. Proteomes of paired serum and saliva samples from healthy controls (HC group, n = 5) and bitches with CMT (CMT group, n = 5) were analysed using a Tandem Mass Tags-based approach. Twenty-five dogs were used to validate serum albumin as a candidate biomarker in an independent sample set. The proteomic analysis quantified 379 and 730 proteins in serum and saliva, respectively. Of those, 35 proteins in serum and 49 in saliva were differentially represented. The verification of albumin in serum was in concordance with the proteomic data, showing lower levels in CMT when compared to the HC group. Some of the modulated proteins found in the present study such as haptoglobin or S100A4 have been related to CMT or human breast cancer previously, while others such as kallikrein-1 and immunoglobulin gamma-heavy chains A and D are described here for the first time. Our results indicate that saliva and serum proteomes can reflect physiopathological changes that occur in CMT in dogs and can be a potential source of biomarkers of the disease.

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