scholarly journals Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2228
Author(s):  
Kai Jin ◽  
Jing Zhou ◽  
Qisheng Zuo ◽  
Jiuzhou Song ◽  
Yani Zhang ◽  
...  
Keyword(s):  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Gallus Gallus ◽  
Future Research ◽  
Biological Processes ◽  
Rna Seq ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Difference ◽  
Go Terms

Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.

scholarly journals Epistaxis as a marker for severe acute respiratory syndrome coronavirus-2 status – a prospective study

2020 ◽  
Vol 134 (8) ◽  
pp. 717-720 ◽  
Author(s):  
MH Hussain ◽  
M Mair ◽  
P Rea
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Prospective Study ◽  
Control Group ◽  
Study Group ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
The Mean ◽  
Polymerase Chain ◽  
The Difference ◽  
A Prospective Study

AbstractObjectiveTo evaluate the prevalence of severe acute respiratory syndrome coronavirus-2 infection in patients presenting with epistaxis to a tertiary otolaryngology unit.MethodsA prospective study was conducted of 40 consecutive patients presenting with epistaxis referred to our tertiary otolaryngology unit. A group of 40 age-matched controls were also included. All patients underwent real-time reverse transcriptase polymerase chain reaction testing for severe acute respiratory syndrome coronavirus-2. Symptoms of fever, cough and anosmia were noted in the study group.ResultsThe mean age was 66.5 ± 22.4 years in the study group. There were 22 males (55 per cent) and 18 females (45 per cent). The mean age in the control group was 66.3 ± 22.4 years (p = 0.935). There were six positive cases for severe acute respiratory syndrome coronavirus-2 (15 per cent) in the epistaxis group and one case (2.5 per cent) in the control group. The difference was statistically significant (p = 0.05).ConclusionEpistaxis may represent a presenting symptom of severe acute respiratory syndrome coronavirus-2 infection. This may serve as a useful additional criterion for screening patients.

scholarly journals Detection of NaturalToxoplasma gondiiInfection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
John Mokua Mose ◽  
John Maina Kagira ◽  
Simon Muturi Karanja ◽  
Maina Ngotho ◽  
David Muchina Kamau ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Human Beings ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Free Range ◽  
Age Dependent ◽  
Polymerase Chain ◽  
The Difference

The detection ofToxoplasma gondiiin free-range chickens is a good indicator of possible risk to human beings. The aim of this study was to investigate the occurrence ofT. gondiiin free-range chicken using polymerase chain reaction (PCR). Brain samples from 105 free-range chickens from three administrative areas in Thika region, Kenya, were collected, DNA-extracted, and analyzed using PCR to detect presence ofT. gondii. The overall prevalence ofT. gondiiin all the three areas was 79.0% (95% CI: 70.0–86.4%) and the prevalence across the three areas was not significantly different (P=0.5088;χ2=1.354). Female chickens had higher (79.4%) prevalence than males (78.6%), although the difference was not significant (P=0.922,χ2= 0.01). However, chickens that were more than 2 years old had significantly (P=0.003;χ2= 11.87) higher prevalence compared to younger ones. The study indicates that there was a high occurrence ofT. gondiiinfection in free-range chickens from Thika region and that the infection rate is age dependent. Further studies should be carried out to determine the possible role of roaming chickens in the epidemiology of the disease among humans in the area.

scholarly journals THE FREQUENCY OF THROMBOTIC COMPLICATIONS AND FEATURES OF GENOTYPES OF POLYMORPHIC MARKERS OF HEMOSTASIS GENES IN CHILDREN WITH NONCOMPACT CARDIOMYOPATHY

2019 ◽  
Vol 20 (3) ◽  
pp. 139-144 ◽  
Author(s):  
E. N. Basargina ◽  
M. K. Umarova ◽  
K. V. Savostyanov ◽  
Yu. V. Derevnina ◽  
I. E. Smirnov
Keyword(s):  
Cardiac Remodeling ◽  
Retrospective Cohort ◽  
Mthfr Gene ◽  
Polymorphic Markers ◽  
Chain Reaction ◽  
Noncompaction Cardiomyopathy ◽  
Thrombotic Complications ◽  
Polymerase Chain ◽  
The Difference ◽  
Folate Cycle

Noncompaction cardiomyopathy (NCMP) is characterized by the anomalous myocardium structure and various types of cardiac remodeling, in some cases it is accompanied by thrombotic complications. Preconditions for thrombosis in the disease are unknown, as also there are differences in thrombosis rates between NCMP and other cardiomyopathies, similarly accompanied by the chronic heart failure and analogous remodeling phenotypes. Aim of study is to reveal the difference in the rate of thrombosis in NCMP and dilated cardiomyopathies (DCMP) in children, and to define differences in the frequency of different genotypes of polymorphic markers in an array of hemostasis genes in the two cardiomyopathies. Methods. There was executed a prospective-retrospective cohort study, included patients from the Cardiac Department of the National Scientific and Practical Center of Children's Health from October 2011 to May 2015. The presence of NCMP was established by echocardiography, alleles and genotypes of polymorphic markers of hemostasis and folate cycle genes were determined by polymerase chain reaction in real-time mode. Results. Thrombotic complications in NCMP children were observed more often than in DCMP cases. There were no differences between NCMP and DCMC patients in the frequency of the polymorphic markers c.1691G>A of the F5 gene (p=0.61) , c.20210G>A of the F2 gene (p=1.0) , c.1565T> C of the ITGB3 gene (p=0.32) , 5G(-675)4G of PLANH1 gene (p=0,52) , G(-455)A of FGB gene (p=0.82) , c.677C>T of MTHFR gene (p=0.11). Conclusion Thrombotic complications in NCMP children occur rather more often than in DCMP cases, studied polymorphic markers of the hemostasis and folate cycle genes do not cause this difference, and this requires continuation of the study.

scholarly journals Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1985 ◽  
Author(s):  
Xiaoke Ping ◽  
Tengyue Wang ◽  
Na Lin ◽  
Feifei Di ◽  
Yangyang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Family ◽  
Vital Role ◽  
Gene Family Evolution ◽  
Rna Seq ◽  
Chain Reaction ◽  
Element Analysis ◽  
Cis Element ◽  
Genome Wide ◽  
Polymerase Chain

Lignin is an important biological polymer in plants that is necessary for plant secondary cell wall ontogenesis. The laccase (LAC) gene family catalyzes lignification and has been suggested to play a vital role in the plant kingdom. In this study, we identified 45 LAC genes from the Brassica napus genome (BnLACs), 25 LAC genes from the Brassica rapa genome (BrLACs) and 8 LAC genes from the Brassica oleracea genome (BoLACs). These LAC genes could be divided into five groups in a cladogram and members in same group had similar structures and conserved motifs. All BnLACs contained hormone- and stress- related elements determined by cis-element analysis. The expression of BnLACs was relatively higher in the root, seed coat and stem than in other tissues. Furthermore, BnLAC4 and its predicted downstream genes showed earlier expression in the silique pericarps of short silique lines than long silique lines. Three miRNAs (miR397a, miR397b and miR6034) target 11 BnLACs were also predicted. The expression changes of BnLACs under series of stresses were further investigated by RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT-PCR). The study will give a deeper understanding of the LAC gene family evolution and functions in B. napus.

scholarly journals Detection of Enterococcus faecalis in Necrotic Teeth Root Canals by Culture and Polymerase Chain Reaction Methods

2007 ◽  
Vol 01 (04) ◽  
pp. 216-221 ◽  
Author(s):  
Dilsah COGULU ◽  
Atac UZEL ◽  
Ozant ONCAG ◽  
Semiha d AKSOY ◽  
Cemal ERONAT
Keyword(s):  
Polymerase Chain Reaction ◽  
Enterococcus Faecalis ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Chain Reaction ◽  
Test Species ◽  
Root Canals ◽  
Significant Difference ◽  
Polymerase Chain ◽  
The Difference

ABSTRACTObjectives: The aim of this study was to investigate the presence of Enterococcus faecalis in endodontic infections in both deciduous and permanent teeth by culture and polymerase chain reaction (PCR) methods.Methods: A total of 145 children aged 5-13 years old were involved in this study. The presence of E. faecalis in necrotic deciduous and permanent teeth root canals was studied using culture and polymerase chain reaction methods.Results: Among 145 molar teeth, 57% (n=83) presented necrotic asymptomatic pulp tissues and were included in this study. Culture and PCR methods detected the test species in 18 and 22 of 83 teeth involved, respectively. E. faecalis was cultured from 8 (18%) of 45 necrotic deciduous teeth and from 10 (26%) of 38 necrotic permanent teeth. PCR detection identified the target species in 10 (22%) and 12 (32%) of necrotic deciduous and permanent teeth respectively. Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively). The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).Conclusions: The results of the present study confirm that both culture and PCR methods are sensitive to detect E. faecalis in root canals. (Eur J Dent 2007;1:216-221)

Grocery Store Genetics

2015 ◽  
Vol 77 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Betsy J. Briju ◽  
Sarah E. Wyatt
Keyword(s):  
Gel Electrophoresis ◽  
Grocery Store ◽  
Simple Experiment ◽  
Chain Reaction ◽  
Mendelian Genetics ◽  
Pcr Product ◽  
Basic Biology ◽  
Polymerase Chain ◽  
The Difference ◽  
Red Coloration

Instructors often present Mendelian genetics and molecular biology separately. As a result, students often fail to connect the two topics in a tangible manner. We have adopted a simple experiment to help link these two important topics in a basic biology course, using red and white onions bought from a local grocery store. A lack of red coloration in white onions is a result of one or more mutations in the color production pathway. This mutation can be seen by the use of polymerase chain reaction (PCR) followed by gel electrophoresis. An absence of an amplified PCR product for one of the genes necessary for color production is associated with a lack of color production – an obvious trait in white onion. The students are able to “see” the difference at the DNA level between the red and white onion.

scholarly journals Investigation of coelectroporation as a method for introducing small mutations into embryonic stem cells.

1992 ◽  
Vol 12 (6) ◽  
pp. 2769-2776 ◽  
Author(s):  
A C Davis ◽  
M Wims ◽  
A Bradley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Selectable Marker ◽  
Embryonic Stem ◽  
Chain Reaction ◽  
Genetic Changes ◽  
Replacement Vector ◽  
Hprt Locus ◽  
Polymerase Chain ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

We have investigated coelectroporation as a method for introducing minor genetic changes into specific genes in embryonic stem cells. A selectable marker (neo) and a targeting replacement vector designed to insert a 4-bp insertion into exon 3 of the mouse hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene were coelectroporated into embryonic stem cells and selected in G418 and 6-thioguanine (6-TG). HPRT-negative clones were obtained at a frequency of approximately 1 per 520 G418r clones. Southern analysis and the polymerase chain reaction were used to demonstrate that 3 of 36 of the 6-TG-resistant clones had the desired 4-bp insertion without any other disruption of the HPRT locus. Initial studies indicated that the other 33 6-TG-resistant clones probably resulted from the targeted integration of a concatemer containing both the targeting construct and the selectable neo gene.

Occurrence and Treatment of Mycoplasma Genitalium in Patients Visiting STD Clinics in Sweden

2000 ◽  
Vol 11 (5) ◽  
pp. 324-326 ◽  
Author(s):  
G Johannisson ◽  
Y Enström ◽  
G-B Löwhagen ◽  
V Nagy ◽  
K Ryberg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Life Time ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
History Of ◽  
Polymerase Chain ◽  
The Difference

Two hundred and thirty-three men and 85 women visiting STD clinics in western Sweden between April 1997 and March 1998 were examined for Mycoplasma genitalium and Chlamydia trachomatis. The bacteria were identified by the polymerase chain reaction (PCR) technique. Three women (3.5%) and 18 men (7%) were positive for M. genitalium. Seventeen (14%) of the 115 men with urethritis were infected but only one of the men was without urethritis. After treatment with tetracyclines for 10 days, one woman and 8 of the 13 men still harboured M. genitalium. M. genitalium-infected men did not have more life-time partners than other men visiting STD clinics. More men positive for M. genitalium gave a history of previous urethritis but the difference was not significant.

scholarly journals COVID-19 in Pakistan

BioMedica ◽  
2020 ◽  
Vol 36 (2S) ◽  
pp. 33-34
Author(s):  
Ghazala Rubi ◽  
Irfan Malik ◽  
Zainab Javaid
Keyword(s):  
Health Professionals ◽  
Action Plan ◽  
World Health ◽  
Future Research ◽  
Chain Reaction ◽  
Public Sector Hospital ◽  
Polymerase Chain ◽  
The Government ◽  
Health Organization ◽  
Effective Reaction

<p>Coronavirus disease-2019 (COVID-19) outbreak was first declared as emergency on January 30th2020 by World Health Organization (WHO). The Government of Pakistan presented a National action plan, which aimed to inhibit the spread of this Corona virus and to reinforce the effective reaction due to this COVID-19 epidemic. Lahore General Hospital (LGH) is the first public sector hospital that started COVID-19 real-time reverse transcription polymerase chain reaction (rRT-PCR) testing to serve community. COVID-19 has affected the mental health of public in general and health professional in particular. We need to support healthprofessionals to do theirjobs, be safe and stay alive. Future research should explore the fears and<br />coping strategies of health professionals during pandemics.</p>

Sign in / Sign up

Export Citation Format

Share Document