scholarly journals Effect of Phlorotannins from Brown Seaweeds on the In Vitro Digestibility of Pig Feed

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2193
Author(s):  
Lauren Ford ◽  
Chloe Curry ◽  
Mairead Campbell ◽  
Katerina Theodoridou ◽  
Gary Sheldrake ◽  
...  
Keyword(s):  
Animal Feed ◽  
Solid Phase ◽  
In Vitro Digestibility ◽  
Gut Health ◽  
Brown Seaweeds ◽  
Positive Effects ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
Pig Feed

Phlorotannins have been reported to have positive effects on pig health, including improved gut health and digestibility. In this study, we investigate the effect of phenolics found in two brown seaweeds, Ascophyllum nodosum and Fucus serratus, on in vitro dry matter digestibility of seaweeds and commercial pig feed. Phlorotannin extracts and whole seaweeds were supplemented into pig feed to test their effect on digestibility. Solid-phase extraction was used to purify the phenolics to phlorotannins. The results showed a slight decrease in the digestibility of pig feed that was found to be significant when phlorotannin extracts were added from either seaweed. However, when whole A. nodosum was added to the pig feed, the effect on digestibility was less pronounced. Specifically, no significant difference in digestibility was observed at inclusion rates up to 5%, and thereafter results varied. A difference in digestibility was also observed in the same species at the same inclusion rate, collected from different seasons. This suggests that other compounds, e.g., polysaccharides, are having an effect on digestibility when whole seaweeds are supplemented to animal feed. This research has also highlighted the need to base supplementation on phenolic concentration as opposed to a standardised percentage inclusion of seaweeds to ensure that digestibility is not adversely affected.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation

2005 ◽  
Vol 48 (spe) ◽  
pp. 161-168 ◽  
Author(s):  
Lúcia de Fátima Araújo ◽  
Ariosvaldo Nunes Medeiros ◽  
Américo Perazzo Neto ◽  
Líbia de Sousa Conrado Oliveira ◽  
Flávio Luiz Honorato da Silva
Keyword(s):  
Dry Matter ◽  
Solid Medium ◽  
Animal Feed ◽  
In Vitro Digestibility ◽  
Cactus Pear ◽  
Protein Supplements ◽  
Dry Matter Digestibility ◽  
Matter Production ◽  
Gross Energy

The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross energy value when compared to that of the cactus pear in natura.

scholarly journals ADDITION OF TOFU WASTE TO CONCENTRAT FEED ON RUMINANT ANIMAL VALUE

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Engkus Ainul Yakin ◽  
Sri Sukaryani ◽  
Catur Suci Purwati ◽  
Desi Lestari
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Animal Feed ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Ruminant Animal ◽  
Significant Difference

The purpose of this study was to determine the effect of the addition of tofu waste in concentrate feed on the nutritional value of ruminant animal feed. Concentrate feed used in this study uses concentrated feed for sheep. Tofu waste was obtained from the place of making tofu in Girimarto sub-district, Wonogiri Regency. The research method used was concentrate feed for sheep given the addition of tofu waste. The study used 3 treatments namely T0 = Concentrate without the addition of tofu waste (control), T1 = Concentrate with 10% tofu waste addition, and T2 = Concentrate with 20% tofu waste addition. The results of the study were analyzed in the laboratory to determine the nutritional value of sheep feed. The study was designed with a completely randomized design. Observation parameters of nutritional value of feed include dry matter, crude protein, crude fiber and in vitro digestibility. The results showed that dry matter content was 78.54 ± 0.23% and crude protein was 16.63 ± 0.46% with the addition of tofu waste until 20% showed a significant difference, whereas crude fiber was 20.37 ± 0.48 % showed no significant difference, while the dry matter digestibility and organic matter digestibility  showed no significant difference. The conclusion of this study was the addition of tofu waste in the concentrate had an effect on the dry matter and crude protein, but not significantly different on crude fiber. The addition of tofu waste to the concentrate did not affect the digestibility of dry matter or the digestibility of organic matter.Key words : Animal feed, concentrate,  in vitro digestibility, tofu waste

scholarly journals In Vitro Digestibility of Lamtoro Leaves (Leucaena Leucocephala) In Poultry With Gizzard Fluid And Duodenum

2021 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Tri Ambarwati ◽  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin
Keyword(s):  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Dry Matter ◽  
Protein Digestibility ◽  
In Vitro Digestibility ◽  
Duodenal Fluid ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Significant Difference

Protein source feed is the feed that has the biggest share in the nutritional needs of livestock. Lamtoro or Leucaena leucocephala is one of the leaf flours that can be used as an alternative feed ingredient for poultry in the tropics. With its abundant availability and does not compete for its needs with humans and has no selling value so that it can minimize consumption costs for poultry. This study aims to determine the effect of fluid from the digestive tract of chickens on the in vitro digestibility measurement of lamtoro leaves. The study used a completely randomized design (CRD) with a unidirectional pattern with 4 treatments and 3 replications. The treatments consisted of P0 = control, P1 = gizzard fluid, P2 = duodenal fluid and P3 = a mixture of gizzard and duodenal fluid. The results of this study show the average Dry Matter Digestibility Coefficient (%) for the treatment P0, P1, P2 and P3 respectively; 19.276, 29.089, 20.515 and 28.737. Organic Matter Digestibility Coefficient ; 27.702, 36.313, 24.795 and 35.649. Dissolved Protein Digestibility Coefficient ; 39.777, 53.288, 21.145 and 48.737. The results of the statistical test that the administration of lamtoro leaves showed a significant difference (P<0.05) on the digestibility coefficient of dry matter, organic matter and dissolved protein. The conclusion is that the use of gizzard liquid is more effective in increasing the in vitro digestibility of lamtoro leaves.

scholarly journals Chemical Composition and Digestibility of Six Species of Fodder (Fabaceae)

2021 ◽  
Vol 13 (12) ◽  
Author(s):  
Eliseo Sosa-Montes ◽  
Sergio Iban Mendoza-Pedroza ◽  
Perpetuo Álvarez-Vázquez ◽  
Pablo Alfredo Domínguez-Martínez ◽  
Ricardo Barcena-Gama ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Animal Feed ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Acacia Angustissima ◽  
Low Levels

Objectives: To determine the chemical composition and in vitro digestibility of Acacia angustissima, Dalea spp., Desmodium spp., Leucaena leucocephala, Phaseolus vulgaris and Tephrosia vicioides (Fabaceae) fodder species. Design/Methodology/Approach: Crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude lignin (CL), cellulose (Cel), hemicellulose (Hcel), cellular content (CC) and in vitro dry matter digestibility (IVDMD) were determined. The design was completely random with three repetitions. The variables were correlated by pairs and the means of the species were compared with Tukey’s test (P<0.05). Results: Dalea spp. had the highest crude protein (17.7%), followed by A. angustissima (15.9%) and L. leucocephala (14.1%). A. angustissima (73.2%), Dalea spp. (74.9%) and P. vulgaris (77.5%) showed the highest IVDMD. L. leucocephala, Tephrosia vicioides and Desmodium spp. showed the lowest values of CP (14.1, 11.8 and 12.3%, respectively) and IVDMD (70.4, 70.2 and 64.9%, respectively). Desmodium spp. showed high levels of NDF (59.2%), ADF (41.4%), CL (17.5%), Cel (29.7%) and Hcel (17.8%) (P?0.05). IVDMD showed positive correlation with CC and negative correlations with NDF, CL, and Hcel (P<0.05). Study Limitations/Implications: Desmodium spp. showed high content of CL and low values of CP and IVDMD, therefore supplements should be added when used in animal feed. Findings/Conclusions: Dalea spp. showed low levels of lignin and high levels of protein and digestibility, making it posible to use as feed for ruminants

scholarly journals In Vitro Study of Fermented Complete Feed by Using Sago Residues as Main Source Diet

2015 ◽  
Vol 17 (3) ◽  
pp. 129
Author(s):  
Samadi Samadi ◽  
Sitti Wajizah ◽  
Yunasri Usman
Keyword(s):  
Animal Feed ◽  
In Vitro Study ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Feed Formulation ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Coconut Meal ◽  
By Products

Recently, fermentation of low quality feed such as agro industry by products has been widely applied to produce enriched animal feed and improve animal productivity. The purpose of this study was to evaluate in vitro digestibility of fermented complete feed by using agro-residues from sago starch processing industries as main source diet.  Feed was formulated on the basis of 40% sago residue and mixed with other ingredients (rice brand, coconut meal, bread by product, soybean meal and soybean hulls) to fulfill the requirement of sheep with 16,10% of CP, 2,80 Mkal ME/kg and TDN 60,88% based on calculation. Mineral and Urea were added in the complete feed to reach mineral and CP requirements of sheep.  Complete feed formulation was fermented by using 3 commercial fermentation products (Saus Burger Pakan® (SBP), Probion® and EM4®) for 21 days. Total and types of microbes from commercial fermentation products which were utilized in this study were not determined. Parameters measured in this study were pH, NH3, IVDMD (in vitro dry matter digestibility), IVOMD (in vitro organic matter digestibility) and VFA total. The model used for the statistical analysis was completely randomized design (CRD) with 4 treatments (control and 3 different commercial fermentation products) and 4 replications.  The results of this study indicated that administration of different commercial fermentation products into fermented complete feed based on sago residues significantly influenced (P<0.05) on pH, NH3, VFA total, IVDMD and IVOMD. Generally, commercial fermentation products mainly SBP produced better feed quality by improving the values of pH, NH3, VFA total, IVDMD and IVOMD. However, types and total microorganisms were needed to be determined before experiment.

PENGARUH PERLAKUAN ALKALI PADA LIMBAH KULIT KOPI DAN PENINGKATAN KECERNAANNYA DENGAN TEKNIK IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
A. Fariani., dkk
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Animal Feed ◽  
Alkali Treatment ◽  
In Vitro Digestibility ◽  
Coffee Pulp ◽  
Dry Matter Digestibility ◽  
Tannin Concentration ◽  
Organic Matter Digestibility

This research was done to improve coffee pulp digestibility with alkali treatment and adding organic acid by in vitro. This research was done in two stage, fi rst stage was soaking in alkali fl uid and second stage was in vitro digestibility analysis, in Animal Feed and Nutrition Laboratory, Faculty of Agriculture, Sriwijaya University. Completely Randomized Design (CRD) used in 4 reatments and feed treatment was replicated 3 times. The treatments were: P0 (control), P1 (2 day soaking), P2 (3 day soaking) and P3 (4 day soaking). Observe parameters were tannin concentration, dry matter digestibility, organic matter digestibility, concentration of N-NH3 and (pH). The result showed that the treatment had nosignifi cant diference (P0.01) on tannin concentration. However, it was signifi cantly difference on dry matter digestibility, organic matter digestibility, concentration of N-NH3, and (pH).Key Words: Coffee pulp, alkali treatment, digestibility, in vitro techniques

scholarly journals The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Monica Canadianti ◽  
Lies Mira Yusiati ◽  
Chusnul Hanim ◽  
Budi Prasetyo Widyobroto ◽  
Andriyani Astuti
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
In Vitro Technique ◽  
Significant Difference ◽  
Organic Matter Digestibility

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.

scholarly journals Effects of Alysacrpus Monilifer fodder intake on feed consumption and performance of desert goats

2021 ◽  
pp. 196-200
Author(s):  
Ebahiem Mohammed Alhadi ◽  
Ajabeldoor Izarig Mubaak Abdalla ◽  
Salah Bukhari Salah Abulgabar ◽  
Abdalla Mohamed Suleiman Eshag ◽  
Hammad Dahia Salah Basar ◽  
...  
Keyword(s):  
Dry Matter ◽  
Plant Biomass ◽  
Feed Consumption ◽  
In Vitro Digestibility ◽  
Average Weight ◽  
Dry Matter Digestibility ◽  
Natural Pasture ◽  
Significant Difference ◽  
And Performance

This study was conducted to study the effects of the intake of the Alysacrpus Monilifera on dry matter intake, digestibility of nutrients and performance of Desert bucks. Twelve Desert goats bucks 4-5 years old with an average weight of 11 + 0.250 kg, were divided into three equal groups. The first group was fed A. Monilifera fodder, while the second group consumed the natural pasture free of A. Monilifera while the third group consumed the groundnut haulms. After observing the cases of deadly diarrhea in the first group, the feeding pattern was modified with 50% natural pasture and 50% A. Monilifer fodder. The fodder was analyzed as well as conducting in vitro digestibility. The experimental design was randomized and the data were analyzed using the analysis of variance and the detection of differences among means using least significant difference test. The results showed that feeding A. Monilifer alone resulted in fatal diarrhea in bucks without affecting appetite, and the dry matter digestibility and organic matter in vitro digestibility was significantly (P<0.05) higher in A. Monilifer than compared to the groundnut haulms or the natural grazing. The weight gain was significantly (P<0.05) higher for the group on groundnut haulms and the natural grazing than those on A. Monilifer alone but when 50% of the plant biomass was replaced by the natural grazing the gain was significantly higher. The study concluded that the intake of A. Monilifer at the growth stage was good quality roughage when fed as part of the diet.

scholarly journals Chemical composition and in vitro digestibility of some selected under-utilized tropical seeds as protein sources in ruminants’ diet

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Oluwatosin Bode Omotoso ◽  
Mary Oluwafunmilayo Adeduntan ◽  
Adebowale Noah Fajemisin
Keyword(s):  
Short Chain Fatty Acids ◽  
Nutritional Composition ◽  
Metabolizable Energy ◽  
In Vitro Digestibility ◽  
Protein Sources ◽  
Dry Matter Digestibility ◽  
Supplementary Feed ◽  
Monodora Myristica

Abstract Background The study highlighted the potential of three common and under-utilized tropical leguminous seeds (Tomentosa nilotica, Dioclea reflexa and Monodora myristica) to be used as supplementary feed to ruminant livestock. These seeds (their plants inclusive) are valuable sources of food and medicine for the prevention of illness and maintenance of human health. The medicinal properties of these seeds include antimicrobial, anti-inflammatory, anti-oxidant and immuno-stimulant. Trypsin inhibitors, which are common anti-nutritional factors in legumes and for monogastric animals, do not exert adverse effects in ruminants because they are degraded in the rumen. Hence, the crux of this study is to examine the effect of processing methods on the nutritional composition (proximate, fibre fractions, minerals, anti-nutrients) and in vitro digestibility of Tomentosa nilotica, Dioclea reflexa and Monodora myristica seeds and their suitability as feedstuff (protein sources) in small ruminant feed, particularly during off-season. Results From the results, raw Tomentosa nilotica and Monodora myristica have the highest crude protein (30.35% CP) and fat (22.40% EE), respectively. It is noteworthy that roasting best improve the mineral and significantly reduce the anti-nutrients observed in this study better compared to boiling and soaking methods. The highest organic matter digestibility, short-chain fatty acids, metabolizable energy and in vitro dry matter digestibility values were obtained in Dioclea reflexa compared to other test seeds. Roasting best improved the nutritive values, while Dioclea reflexa seed was rated highest for all the nutritional attributes and in vitro digestibility. Conclusions Dioclea reflexa could be incorporated in ruminants’ diet as protein source, particularly during the off-season, for improved ruminant production in Nigeria. However, in vivo study is therefore recommended to validate this report.

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