scholarly journals Influence of Parental Fatty Acid Desaturase 2 (fads2) Expression and Diet on Gilthead Seabream (Sparus aurata) Offspring fads2 Expression during Ontogenesis

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2191
Author(s):  
Hanlin Xu ◽  
Shajahan Ferosekhan ◽  
Serhat Turkmen ◽  
Juan Manuel Afonso ◽  
María Jesús Zamorano ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Polyunsaturated Fatty Acid ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Chain Polyunsaturated Fatty Acid ◽  
Nutritional Programming ◽  
Heart Beating ◽  
Long Chain

Previous studies have shown that it is possible to increase the ability of marine fish to produce long-chain polyunsaturated fatty acid from their 18C precursors by nutritional programming or using broodstock with a higher fatty acyl desaturase 2 (fads2) expression. However, those studies failed to show the effect of these interventions on the expression of the fads2 gene in the developing egg. Moreover, there were no studies on the temporal expression of the fads2 during ontogeny in the gilthead sea bream (Sparus aurata). In order to determine the changes in expression of fads2 during ontogeny, gilthead sea bream broodstock with a high (HRO) or low (LRO) fads2 expression fed a diet previously used for nutritional programming, or a fish oil-based diet (LFO) were allowed to spawn. The samples were taken at the stages of spawning, morula, high blastula, gastrula, neurula, heart beating, hatch and 3 day-old first exogenous feeding larvae to determine fads2 expression throughout embryonic development. The results showed the presence of fads2 mRNA in the just spawned egg, denoting the maternal mRNA transfer to the developing oocyte. Later, fads2 expression increased after the neurula, from heart beating until 3-day-old larvae, denoting the transition from maternal to embryonic gene expression. In addition, the eggs obtained from broodstock with high fads2 expression showed a high docosahexaenoic acid content, which correlated with the downregulation of the fads2 expression found in the developing embryo and larvae. Finally, feeding with the nutritional programming diet with the partial replacement of fish oil by rapeseed oil did not affect the long chain polyunsaturated fatty acid (LC-PUFA) contents nor fads2 expression in the gilthead sea bream developing eggs.

scholarly journals Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata)

2017 ◽  
Vol 118 (7) ◽  
pp. 500-512 ◽  
Author(s):  
Serhat Turkmen ◽  
Maria J. Zamorano ◽  
Hipólito Fernández-Palacios ◽  
Carmen M. Hernández-Cruz ◽  
Daniel Montero ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Developmental Stages ◽  
Sparus Aurata ◽  
Linseed Oil ◽  
Regulation Of Gene Expression ◽  
Fish Growth ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Nutritional Programming ◽  
Long Chain

AbstractNutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and low-fishmeal (FM) diets for 1 month. The present study further investigated the effects of broodstock feeding on the same offspring when they were 16 months old and were challenged for a second time with the low-FM and low-FO diet for 2 months. The results showed that replacement of parental moderate-FO feeding with LO, combined with juvenile feeding at 4 months old with low-FM and low-FO diets, significantly (P<0·05) improved offspring growth and feed utilisation of low-FM/FO diets even when they were 16 months old: that is, when they were on the verge of their first reproductive season. Liver fatty acid composition was significantly affected by broodstock or reminder diets as well as by their interaction. Moreover, the reduction of long-chain PUFA and increase in α-linolenic acid and linoleic acid in broodstock diets lead to a significant down-regulation of hepatic lipoprotein lipase (P<0·001) and elongation of very long-chain fatty acids protein 6 (P<0·01). Besides, fatty acid desaturase 2 values were positively correlated to hepatic levels of 18 : 4n-3, 18 : 3n-6, 20 : 5n-3, 22 : 6n-3 and 22 : 5n-6. Thus, this study demonstrated the long-term nutritional programming of gilthead sea bream through broodstock feeding, the effect of feeding a ‘reminder’ diet during juvenile stages to improve utilisation of low-FM/FO diets and fish growth as well as the regulation of gene expression along the fish’s life-cycle.

scholarly journals Fish oil–based finishing diets strongly increase long‐chain polyunsaturated fatty acid concentrations in farm‐raised common carp ( C yprinus carpio L.)

2014 ◽  
Vol 46 (9) ◽  
pp. 2174-2184 ◽  
Author(s):  
Sebastian Schultz ◽  
Apostolos‐Manuel Koussoroplis ◽  
Zhara Changizi‐Magrhoor ◽  
Jörg Watzke ◽  
Martin J Kainz
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Common Carp ◽  
Polyunsaturated Fatty Acid ◽  
Chain Polyunsaturated Fatty Acid ◽  
Finishing Diets ◽  
Long Chain

scholarly journals Adaptation of lipid metabolism, tissue composition and flesh quality in gilthead sea bream (Sparus aurata) to the replacement of dietary fish oil by linseed and soyabean oils

2004 ◽  
Vol 92 (1) ◽  
pp. 41-52 ◽  
Author(s):  
D. Menoyo ◽  
M. S. Izquierdo ◽  
L. Robaina ◽  
R. Ginés ◽  
C. J. Lopez-Bote ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fish Oil ◽  
Acid Composition ◽  
Vegetable Oil ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Flesh Quality

Linseed (LO) and soyabean (SO) oils were evaluated as fish-oil (FO) substitutes in the diets of marketable-sized gilthead sea bream (Sparus aurata). Practical diets were designed factorially with the lipid added as follows (%): FO 100, LO 60+FO 40, LO 80+FO 20, SO 60+FO 40, SO 80+FO 20. The effects of experimental diets on growth, fatty acids patterns in liver and muscle, flesh quality variables and activities of selected enzymes involved in lipid synthesis and catabolism were determined at the end of a 7-month trial. Fatty acid composition of liver and muscle generally reflected the fatty acid composition of the diets. Then−3 PUFA levels were significantly reduced by the inclusion of vegetable oils. This tendency was more pronounced for EPA than for docosahexaenoic acid. Then−3:n−6 fatty acid ratio reached the lowest values in fish fed the SO diets; this was associated with a higher liver lipid deposition. No differences were found in fillet texture and pH. However, under conditions of forced peroxidation, muscles from fish fed the SO diets had lower peroxidation levels. Vegetable oil substitution decreased lipogenesis in liver and this effect was greatest at the highest substitution level. In contrast, muscle β-oxidation enzymes had increased activities with vegetable oil substitution. Thus, the lower hepatic lipogenesis was correlated with an increased lipid utilisation in muscle. It is concluded that growth and lipid metabolism were affected by experimental diets.

scholarly journals The Use of Low-EPA Fish Oil for Long-Chain Polyunsaturated Fatty Acid Supplementation of Preterm Infants

2000 ◽  
Vol 48 (6) ◽  
pp. 835-841 ◽  
Author(s):  
Alexandre Lapillonne ◽  
Jean-Charles Picaud ◽  
Véronique Chirouze ◽  
Joëlle Goudable ◽  
Bernadette Reygrobellet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Preterm Infants ◽  
Polyunsaturated Fatty Acid ◽  
Chain Polyunsaturated Fatty Acid ◽  
Fatty Acid Supplementation ◽  
Acid Supplementation ◽  
Long Chain
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