scholarly journals FSH Stimulation with Short Withdrawal Improves Oocyte Competence in Italian Mediterranean Buffalo (Bubalus bubalis)

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1997
Author(s):  
Georgios Petrovas ◽  
Michal Andrzej Kosior ◽  
Giorgio Antonio Presicce ◽  
Marco Russo ◽  
Gianluigi Zullo ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Oocyte Competence

The aim of this work was to evaluate the efficiency of different FSH doses and FSH coasting times before ovum pick-up (OPU) on follicular growth and oocyte competence in buffalo. Experiment 1 involved two different FSH treatments: 40 mg FSH given three (FSH3) or six (FSH6) times, 2 days after dominant follicle removal were tested, with OPU carried out after 40–44 h of coasting. In experiment 2, OPU was carried out after FSH6 protocol followed by 28–32 h (C1), 40–44 h (C2), or 64–68 h (C3) of coasting time. Cumulus oocyte complexes (COCs) were classified, in vitro matured, fertilized, and cultured. The results demonstrated that FSH6 increased the total number of follicles, the number and percentages of medium and large follicles, the number and the proportion of good quality oocytes, and the number of grade 1,2 and fast-developing blastocysts compared to the control. C3 decreased the percentage of good quality oocyte and blastocyst rates compared to C1 and C2. A higher percentage of fast blastocysts and average number of grade 1,2 blastocysts was observed in C1 compared to C3, with intermediate values found in C2. The improved efficiency in terms of blastocyst yields suggests the use of FSH6 + C1 protocol for ovarian superstimulation in buffalo.

135 Use and dose of porcine follicle-stimulating hormone for ovarian superstimulation prior to ovum pickup and in vitro embryo production in pregnant Holstein heifers

2019 ◽  
Vol 31 (1) ◽  
pp. 192
Author(s):  
R. V. Sala ◽  
L. C. Carrenho-Sala ◽  
M. Fosado ◽  
E. Peralta ◽  
D. C. Pereira ◽  
...  
Keyword(s):  
Limited Information ◽  
Dominant Follicle ◽  
Blastocyst Development ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Oocyte Competence ◽  
Treatment Groups ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

The benefit of superstimulation with exogenous FSH before ovum pickup for in vitro embryo production has been the subject of significant controversy. In addition, there is limited information on different dose regimens. Thus, the objective of the present study was to evaluate the effect of dose of porcine (p)-FSH during superstimulation before ovum pickup (OPU) on in vitro embryo production in pregnant heifers. Pregnant Holstein heifers (n=36) were assigned to a complete 3×3 crossover design. Three treatment groups were evaluated as follows: p-FSH 0mg (FSH0), p-FSH 160mg (FSH160) and p-FSH 300mg (FSH300). Three sessions of OPU were performed on each animal at 48, 62 and 76 days of gestation, with a washout interval between sessions of 14 days. Follicular wave emergence was synchronized by dominant follicle removal. Heifers in the FSH0 group received no further treatment, whereas the remaining groups received a total of 4 injections 12h apart as follows: FSH160 (48.0, 42.7, 37.3 and 32.0mg) or FSH300 (90.0, 80.0, 70.0 and 60.0mg), beginning 36h after dominant follicle removal. Ovum pickup was performed in all heifers 40h after the last p-FSH injection. Heifers were subjected to OPU for oocyte recovery, and number of follicles was determined. Recovered oocytes were processed and in vitro embryo production performed. Differences between treatment groups were evaluated by generalized linear mixed models. Data are presented (Table 1) as mean±standard error of the mean. There was no effect of days in gestation for any of the outcomes evaluated (P>0.05). Follicle numbers at the time of oocyte recovery were different (P<0.01) between groups. Heifers in the FSH300 group had a greater (P<0.05) number of medium, large and total follicles than heifers in the FSH0 group, whereas heifers in the FSH160 were intermediate. Total number of recovered, viable and cleaved oocytes were greater (P<0.01) in FSH300- than in FSH160- and FSH0-treated heifers. Cleavage rate and blastocyst development rate were not different (P>0.10) between groups. The number of grade 1 and 2 blastocysts was greater in FSH300- than in FSH160- and FSH0-treated heifers (P<0.03). In summary, the use of 300mg of p-FSH before OPU in pregnant heifers increases the number of follicles, oocytes and blastocysts produced per heifer with no detrimental effect on oocyte competence. Table 1.Ovum pickup and in vitro embryo production in pregnant heifers treated with different doses of porcine FSH

scholarly journals Follicular growth and oocyte competence in the in vitro cultured mouse follicle: effects of gonadotrophins and steroids

2007 ◽  
Vol 14 (2) ◽  
pp. 75-83 ◽  
Author(s):  
A. A. Murray ◽  
A. K.E. Swales ◽  
R. E. Smith ◽  
M. D. Molinek ◽  
S. G. Hillier ◽  
...  
Keyword(s):  
Follicular Growth ◽  
Oocyte Competence

scholarly journals Angiotensin II Signaling Promotes Follicle Growth and Dominance in Cattle

Endocrinology ◽  
2011 ◽  
Vol 152 (12) ◽  
pp. 4957-4965 ◽  
Author(s):  
Rogério Ferreira ◽  
Bernardo Gasperin ◽  
Monique Rovani ◽  
Joabel Santos ◽  
Marcos Barreta ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Granulosa Cell ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Follicle Growth ◽  
Angiotensin Ii Signaling

It is generally understood that angiotensin II (AngII) promotes follicle atresia in rats, although recent data suggested that this may not be true in cattle. In this study, we aimed to determine in vivo whether AngII alters follicle development in cattle, using intrafollicular injection of AngII or antagonist into the growing dominant follicle or the second largest subordinate follicle. Injection of saralasin, an AngII antagonist, into the growing dominant follicle inhibited follicular growth, and this inhibitory effect was overcome by systemic FSH supplementation. Injection of AngII into the dominant follicle did not affect follicular growth, whereas injection of AngII into the second largest follicle prevented the expected atresia of this subordinate follicle, and the treated follicle grew at the same rate as the dominant follicle for the next 24 h. Inhibition of AngII action in the dominant follicle decreased estradiol concentrations in follicular fluid and the abundance of mRNA encoding aromatase, 3β-hydroxysteroid dehydrogenase, LH receptor, and cyclinD2 in granulosa cells, with minimal effects on theca cells. The effect of AngII on aromatase mRNA levels was confirmed using an in vitro granulosa cell culture system. In conclusion, these data suggest that AngII signaling promotes follicle growth in cattle and does so by regulating genes involved in estradiol secretion and granulosa cell proliferation and differentiation.

Folliculogenesis in buffalo (Bubalus bubalis): a review

2002 ◽  
Vol 14 (5) ◽  
pp. 315 ◽  
Author(s):  
R. S. Manik ◽  
P. Palta ◽  
S. K. Singla ◽  
V. Sharma
Keyword(s):  
Bubalus Bubalis ◽  
Lower Number ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Lack Of Information ◽  
Synchronous Development ◽  
Second Wave ◽  
Ovarian Follicular Growth ◽  
Selection Of ◽  
Follicular Dynamics

The urgent need for improving the reproductive performance of buffalo necessitates a better understanding of the mechanisms controlling ovarian follicular growth and development. Attention needs to be focused on improving superovulation responses and conception rates, and reducing the variability in ovulation rate and embryo loss. Application of ultrasonic imaging has revealed that follicular turnover during an unstimulated oestrous cycle occurs in waves, with each wave involving synchronous development of a group of follicles, one dominant and several subordinate follicles. There is a predominance of two waves with the first wave beginning around Day 0 (day of ovulation) and the second wave around Day 9 or 10. Primary reasons for a lower superovulation response in buffalo compared with that in cattle is a lower number of primordial and antral follicles, a slower shift from small to large follicles during superovulation, a higher incidence of deep atresia and inability of several large follicles to ovulate, especially when superovulation is induced by equine chorionic gonadotrophin treatment. There is near complete lack of information in Bubalus bubalis on the factors controlling the selection of the dominant follicle, the period of functional dominance and the effects of environmental factors, such as climate and nutrition, on follicular dynamics.

scholarly journals Chorionic Gonadotropin Secretion Is Associated with an Inhibition of Follicular Growth and an Improvement in Oocyte Competence for In Vitro Maturation in the Mare1

1998 ◽  
Vol 58 (3) ◽  
pp. 760-768 ◽  
Author(s):  
Ghylène Goudet ◽  
Laurent Leclercq ◽  
Jacqueline Bézard ◽  
Guy Duchamp ◽  
Daniel Guillaume ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
In Vitro Maturation ◽  
Follicular Growth ◽  
Gonadotropin Secretion ◽  
Oocyte Competence

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

GREM1, EGFR, and HAS2; the oocyte competence markers for improved buffalo embryo production in vitro

2016 ◽  
Vol 86 (8) ◽  
pp. 2004-2011 ◽  
Author(s):  
Rahul Bhardwaj ◽  
Mohd Matin Ansari ◽  
Sriti Pandey ◽  
Mehtab S. Parmar ◽  
Vikash Chandra ◽  
...  
Keyword(s):  
Embryo Production ◽  
Oocyte Competence
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