scholarly journals Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1929
Author(s):  
Mohamed El-Tholoth ◽  
Michael G. Mauk ◽  
Yasser F. Elnaker ◽  
Samah M. Mosad ◽  
Amin Tahoun ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Low Cost ◽  
Skin Lesions ◽  
Nucleic Acid Amplification ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Molecular Assay ◽  
Lateral Flow Strip ◽  
Cutaneous Warts

Bovine papillomatosis is a viral disease of cattle causing cutaneous warts. A diagnosis of this viral infection is very mandatory for combating the resulting economic losses. Given the limited data available about bovine papillomavirus (BPV) in Egypt, the present study involved the molecular diagnosis of bovine papillomavirus type-1 (BPV-1), -2, -4, -5, and -10 in cattle presenting cutaneous warts on the head and neck from New Valley Province, Egypt. The phylogenetic analysis of the detected types of BPV was also performed, followed by developing a point-of-need molecular assay for the rapid identification of identified BPV types. In this regard, a total of 308 cattle from private farms in Egypt were clinically examined, of which 13 animals presented cutaneous warts due to suspected BPV infection. The symptomatic animals were treated surgically, and biopsies from skin lesions were collected for BPV-1, -2, -4, -5, and -10 molecular identification using polymerase chain reaction (PCR). The presence of BPV-1 DNA was confirmed in 11 collected samples (84.6%), while BPV-2, -4, -5, and -10 were not detected. Sequencing of the PCR products suggested the Egyptian virus is closely related to BPV found in India. An isothermal nucleic acid amplification test (NAAT) with labeled primers specific for the BPV-1 L1 gene sequence, and based on recombinase polymerase amplification (RPA), in combination with a lateral flow strip assay for the detection of RPA products, was developed and tested. The point-of-need molecular assay demonstrated a diagnostic utility comparable to PCR-based testing. Taken together, the present study provides interesting molecular data related to the occurrence of BPV-1 in Egypt and reveals the genetic relatedness of the Egyptian BPV-1 with BPV-1 found in buffalo in India. In addition, a simple, low-cost combined test was also validated for diagnosis of the infection. The present study suggests the necessity of future investigations about the circulating strains of the virus among the cattle in Egypt to assess their genetic relatedness and better understand the epidemiological pattern of the disease.

scholarly journals Phylogenetic analysis of a partial L1 gene from bovine papillomavirus type 1 isolated from naturally occurring papilloma cases in the northwestern region of Turkey

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
M. Ozkan Timurkan ◽  
M. Eray Alcigir
Keyword(s):  
Phylogenetic Analysis ◽  
Histopathological Examination ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Sequencing Analysis ◽  
Molecular Characterisation ◽  
Northwestern Turkey ◽  
Northwestern Region

This study was aimed at the molecular characterisation of bovine papillomavirus type 1 (BPV-1) isolated from papilloma cases in the northwestern region of Turkey. BPV-1 is a widely occurring oncogenic virus in cattle and is associated with benign epithelial neoplasia which causes significant economic losses in dairy and beef cattle because of treatment costs. In this study, 29 suspected papilloma specimens were collected from cattle in northwestern Turkey. These samples underwent molecular characterisation via the polymerase chain reaction (PCR) and sequencing analysis as well as macroscopic and histopathological examination. The histopathological examinations confirmed papilloma as the main lesion type in the specimens. Of the 29 papilloma-like tissue samples that were collected, 11 (i.e. 37.93%) were detected as positive and determined as containing BPV-1 (11 of 11, 100%). Using a partial sequence for the L1 gene acquired from GenBank, phylogenetic analysis confirmed the presence of BPV-1 and revealed that the infection might have originated in cross bred domestic and imported cattle. This study provides potentially useful information on the origin and spread of this disease. Its results can potentially aid in the development of appropriate control measures and therapeutic or vaccination strategies against the BPV-1 strain of bovine papillomatosis.

scholarly journals Detection of Orf Virus and Papillomavirus Out of Samples from Goats and Cattle which were Gathered by Multiplex PCR Romania

2012 ◽  
Vol 45 (2) ◽  
pp. 85-88
Author(s):  
Oana Strugaru ◽  
Elena Velescu ◽  
T. Perianu ◽  
Florentina Bocăneţi ◽  
Alessandra Scagliarini
Keyword(s):  
Multiplex Pcr ◽  
Skin Lesions ◽  
Etiologic Agent ◽  
Laboratory Method ◽  
The Body ◽  
Orf Virus ◽  
Economic Losses ◽  
Viral Agent ◽  
Bovine Papillomavirus ◽  
Virus Identification

Detection of Orf Virus and Papillomavirus Out of Samples from Goats and Cattle which were Gathered by Multiplex PCR RomaniaThe lesions which appear on the skin and are caused by parapoxvirus, herpesviruses and papillomavirus, depending on they're gravity, can produce large economic losses. The identification of viral species with different laboratory methods can facilitate making a correct diagnostic in identifying the infectious entity. The most common encountered injuries, especially on goats and kids, have an oral location, breasts, eyes and genital and podal. On the other side on cattles infected with papillomavirus the lesions are multiple and spread all over the body. To identify the etiologic agent from goats and cattle with skin lesions, tissue samples were collected and scabs. The virus identification was made with molecural biology techniques. The PCR method revealed the viral DNA, thus identifying a portion of the B2L preserved gene which is then amplified using primers PPP1-PPP4. For papillomavirus detection, primers FAP59 and FAP 64 were used to conserve the L1 human gene, primers that can help detect other types of papillomavirus from other species. The PCR results showed that lesions from goats were made by Orf virus, a virus that belongs to the papillomavirus gene, as for cattles the bovine papillomavirus. Studies have shown that PCR is a rapid laboratory method which can be used to identify the viral agent that causes severe skin lesions.

scholarly journals Detection and whole genomic characterization of Bovine Papillomavirus type 1 associated with severe mammary cutaneous warts in Turkey

2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Tuba Çiğdem Oğuzoğlu ◽  
Bahattin Taylan Koç ◽  
Zeynep Akkutay-Yoldar ◽  
Seçkin Salar ◽  
Ayhan Baştan
Keyword(s):  
Mammary Gland ◽  
Diagnostic Methods ◽  
Bovine Papillomavirus ◽  
Dominant Type ◽  
Natural Hosts ◽  
Genomic Characterization ◽  
Full Length Genome ◽  
Cutaneous Warts

Papilloma viruses (PVs) induce epithelial tumors in their natural hosts. These tumors are located on the mammary gland of cattle; therefore, PVs are considered viral agents with significant effects, both economical and on the animals’ health. Also, knowing the diversity of bovine PV (BPV) and its prevalence in countries worldwide is important to develop optimal diagnostic methods, as well as methods to fight this infection.The aim of the present study was to characterize the full-length genome of BPV 1 as the dominant type circulating among cattle in Turkey. It is the authors’ opinion that the results of this work provide useful information for further studies on the preventative steps that can be taken against BPV infection.

scholarly journals Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mohammed A. Hamad ◽  
Ahmed M. Al-Shammari ◽  
Shoni M. Odisho ◽  
Nahi Y. Yaseen
Keyword(s):  
Phylogenetic Analysis ◽  
Histopathological Examination ◽  
Complete Sequence ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Sequencing Analysis ◽  
First Report ◽  
Malignant Lesions

This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies.

scholarly journals BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

2013 ◽  
Vol 44 (1) ◽  
pp. 61 ◽  
Author(s):  
Roberta Cotugno ◽  
Dario Gallotta ◽  
Morena d’Avenia ◽  
Annunziata Corteggio ◽  
Gennaro Altamura ◽  
...  
Keyword(s):  
Bovine Papillomavirus ◽  
Death Signals
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