scholarly journals Evaluation of Changes in the Levels of Firmicutes and Bacteroidetes Phyla of Sheep Feces Depending on the Breed

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1901
Author(s):  
Paulina Cholewińska ◽  
Magdalena Wołoszyńska ◽  
Marta Michalak ◽  
Katarzyna Czyż ◽  
Witold Rant ◽  
...  
Keyword(s):  
Body Weight ◽  
Environmental Conditions ◽  
Polymerase Chain Reaction Analysis ◽  
Bacterial Populations ◽  
Bacterial Phyla ◽  
Body Weights ◽  
Breed Differences ◽  
Polymerase Chain ◽  
Similar Body ◽  
Sheep Feces

Studies carried out so far have indicated the effect of the microbiome on the composition of ruminant products. Recent studies have shown that not only diet, but also genetic factors can affect the microbiological composition of the digestive system. The aim of the study was to determine the differences in the levels of selected bacterial phyla in terms of breed differences. Three sheep breeds, i.e., Olkuska, Romanov, and old-type Polish Merino, differing in their use (meat–wool, meat, prolificacy) and country of breed origin were included in the study. Sheep at the same age and of the same sex were kept for a period of 3 months in the same environmental conditions and fed the same feed in the same proportions. The study included real-time PCR (polymerase chain reaction) analysis of feces collected before the slaughter and measurements of body weight and chilled carcasses. The obtained results showed significant differences between the breeds in the levels of bacterial populations tested. There were also differences in body weight between the breeds during the first weight measurements, however, the final results did not show any differences—after three months of maintenance all of them reached similar body weights, despite differences in fecal microbiological composition. The study suggests that in addition to diet and environmental conditions, the microbiology can also be influenced by breed.

scholarly journals PSIII-30 Feedlot performance and carcass characteristics of Angus and Wagyu sired cattle raised to a similar body weight endpoint

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 259-259
Author(s):  
Jerad R Jaborek ◽  
Alejandro E Relling
Keyword(s):  
Body Weight ◽  
Mixed Model ◽  
Block Design ◽  
Random Effect ◽  
Design Experiment ◽  
Feedlot Performance ◽  
Incomplete Block Design ◽  
Breed Differences ◽  
Effect Of Treatment ◽  
Similar Body

Abstract Angus and Wagyu sired calves were used in an incomplete block design experiment to investigate breed differences for growth at a similar body weight. Cattle were individually fed a common corn silage based growing diet for 4 months and transitioned to a common finishing diet for the remainder of the study. Treatments were as follows: T1) Angus sired steers (n = 13) harvested at 612 kg, T2) Wagyu (LMR Fukutsuru 729T) sired steers (n = 4) and heifers (n = 5) harvested at 612 kg, T3) Wagyu (OW Yasufuku 229Y) sired steers (n = 5) and heifers (n = 8) harvested at 612 kg. Data were analyzed in SAS using the mixed model: Yij = μ + Ti + sj + eij, where Ti was the fixed effect of treatment, sj was the random effect of sex, and eij was the random error. Contrasts were made to compare T1 vs. T2 and T3, and T2 vs. T3. Receiving weight was greater (P ≤ 0.02) for Angus sired cattle compared to Wagyu sired cattle, and Fukutsuru sired cattle had greater (P ≤ 0.05) receiving weights compared to Yasufuku sired cattle. Off-test weights were similar across treatments (P = 0.49). Wagyu sired cattle required more days on feed (P ≤ 0.01), resulting in a greater age at harvest (P ≤ 0.01) compared to Angus sired cattle. Fukutsuru sired cattle tended to require fewer days on feed (P = 0.07) and be younger at harvest (P = 0.10) compared to Yasufuku sired cattle. Angus sired cattle had greater ADG (P ≤ 0.01), DMI (P ≤ 0.01), and G:F (P ≤ 0.02) compared to Wagyu sired cattle. Angus sired cattle had a lesser percentage of KPH fat (P ≤ 0.01) and tended to have more desirable YG scores (P = 0.06) compared to Wagyu sired cattle. Wagyu sired cattle had greater marbling scores (P ≤ 0.01) compared to Angus sired cattle, while Yasufuku sired cattle had greater marbling scores compared to Fukutsuru sired cattle.

scholarly journals Hyperhomocysteinemia Induced by Methionine Excess is Effectively Suppressed by Betaine in Geese

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1642
Author(s):  
Zhi Yang ◽  
Yu Yang ◽  
Jinjin Yang ◽  
Xiaoli Wan ◽  
Haiming Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Control Diet ◽  
Control Group ◽  
Lower Plasma ◽  
Protein Levels ◽  
Myocardial Apoptosis ◽  
Serum Homocysteine ◽  
Body Weights ◽  
Similar Body

The objective of our study was to investigate the effects of excess Methionine (Met) on the growth performance, serum homocysteine levels, apoptotic rates, and Bax and Bcl-2 protein levels in geese and to study the role of Bet (betaine) in relieving excess Met-induced hyperhomocysteinemia (HHcy). In this study, 150 healthy male 14-day-old Yangzhou geese of similar body weight were randomly distributed into three groups with five replicates per treatment and 10 geese per replicate: the control group (fed a control diet), the Met toxicity group (fed the control diet +1% Met), and the Bet detoxification group (fed the control diet +1% Met +0.2% Bet). At 28, 49, and 70 d of age, the geese in the Met toxicity group had significantly lower body weights than those in the control group (p < 0.05). The serum homocysteine levels in geese at 70 d of age in the detoxification group were significantly lower than those in the Met toxicity group (p < 0.05). Compared with the control, Met significantly increased cardiomyocyte apoptosis rates, while Bet reduced them. In conclusion, our results suggest that excess methionine reduces body weight induced by myocardial apoptosis, and Bet can be used to effectively lower plasma homocysteine levels.

ASSOCIATION OF KARYOTYPE WITH BODY WEIGHT AND RESISTANCE TO DESICCATION IN DROSOPHILA PSEUDOOBSCURA

1971 ◽  
Vol 13 (1) ◽  
pp. 63-69 ◽  
Author(s):  
J. A. Thomson
Keyword(s):  
Body Weight ◽  
Relative Humidity ◽  
Environmental Conditions ◽  
Gene Arrangement ◽  
Drosophila Pseudoobscura ◽  
Relative Resistance ◽  
Body Weights

The effect of karyotype on body weight and resistance to desiccation has been examined for the Standard (ST) and Chiricahua (CH) gene arrangements of Drosophila pseudoobscura obtained from Mather, California. Mean body weights fall in the order ST/CH > CH/CH > ST/ST, and variability of body weight in the order CH/CH > ST/ST > ST/CH in adult flies of both sexes. The relative resistance to desiccation in mass survival tests at 0% relative humidity, without food, is [Formula: see text] in males and [Formula: see text] in females. Karyotype has an effect on survival under desiccation, apart from its effect on body size.The data provide a further example of pronounced heterosis associated with a gene-arrangement polymorphism under extreme environmental conditions.

The body composition of growing sheep during milk feeding, and the effect on composition of weaning at various body weights

1976 ◽  
Vol 86 (3) ◽  
pp. 483-493 ◽  
Author(s):  
T. W. Searle ◽  
D. A. Griffiths
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Tritiated Water ◽  
Body Weight Loss ◽  
The Body ◽  
Whole Body ◽  
Protein Energy ◽  
Body Weights ◽  
Similar Body ◽  
Milk Feeding

SummaryThirty-four (13♂, 21♀) entire cross-bred lambs were suckled on reconstituted, spray-dried cows' milk from 2 days of age for varying periods of time up to 18 weeks before being weaned on to solid food or slaughtered to determine chemical composition. The body composition of each sheep (water, fat, protein, energy) was also estimated from the tritiated water (TOH) space at 3-weekly intervals during milk feeding, at intervals of 5–8 weeks subsequently and at slaughter. Comparison between. TOH estimates and whole body analysis confirmed the accuracy of the previously-derived prediction equations.The relationship between each of the various body components and body weight for individual sheep during milk feeding was described by a ‘bent’ (non-rectangular) hyperbola. There were no substantial differences between animals in either the position or slope of the lower asymptote nor in the slope of the upper asymptote. There were, however, substantial differences in the point of intersection of the two asymptotes both within and between sexes. The mean intersection points for males and females were 17·7 and 14·4 kg body weight respectively.Sheep weaned at body weights of 11–16 kg subsequently followed the previously defined pattern of growth. Weaning at higher body weights (21–34 kg, body fat not less than 5·5 kg) resulted initially in both fat and/or body weight loss. In the final fattening phase of growth early- and late-weaned sheep of the same sex had similar body composition but females were fatter than males.

Phenotypic characterization of three strains of indigenous goats in Tanzania

2000 ◽  
Vol 28 ◽  
pp. 43-51 ◽  
Author(s):  
M.A. Madubi ◽  
G.C. Kifaro ◽  
P.H. Petersen
Keyword(s):  
Body Weight ◽  
The Body ◽  
Colour Pattern ◽  
Phenotypic Characterization ◽  
Body Measurements ◽  
Body Weights ◽  
Indigenous Goats ◽  
Similar Body

SummaryCharacterization of three strains of indigenous goats found in three regions of Tanzania was undertaken on the basis of their phenotypes viz: body weight and measurements, colour pattern, ears and horns.The three strains differed in the body weights and in the frequencies of the different coat colours. The Dodoma and Mtwara strains had similar body measurements, whereas the Kigoma strain was significantly smaller.

scholarly journals Rumen Bacterial Community of Grazing Lactating Yaks (Poephagus grunniens) Supplemented with Concentrate Feed and/or Rumen-Protected Lysine and Methionine

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2425
Author(s):  
Hu Liu ◽  
Hui Jiang ◽  
Lizhuang Hao ◽  
Xuliang Cao ◽  
Allan Degen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Volatile Fatty Acids ◽  
Rumen Microbiota ◽  
Bacterial Phyla ◽  
Protein Requirements ◽  
Natural Pasture ◽  
Ruminal Microbiota ◽  
Similar Body ◽  
Supplementary Feed

Traditionally, yaks graze only natural pasture all year round without supplements. Forage intake of lactating yaks is below energy and protein requirements, even in the summer, and suckling yaks lose a substantial amount of significant body weight. Today, to mitigate the loss in body weight, supplementary feed is being offered to lactating yaks. However, the effects of supplementary feed on ruminal bacterial communities in lactating yaks is unknown. In the current study, we examined the effect of supplementary feed on ruminal microbiota, using 16S rRNA sequencing, and on volatile fatty acids (VFAs). Twenty-four lactating yaks of similar body weight (218 ± 19.5 kg) and grazing natural pasture were divided randomly into four groups and received different supplements: (1) rumen-protected amino acids (RPA); (2) concentrate feed (C); (3) RPA plus C (RPA+C); and (4) no supplements (control-CON). The concentrations of total VFAs, acetate, and butyrate were greater (p < 0.05) when supplemented with concentrate feed (C and RPA+C) than without concentrate feed (CON and RPA). Bacteroidetes (B) and Firmicutes (F) were the dominant ruminal bacterial phyla in all groups. The ratio of relative abundance of F:B in RPA+C was greater than in the RPA group, while there was no difference between CON and RPC (interaction, p = 0.026). At the genus level, the relative abundances of Absconditabacteriales_SR1, Bacteroidales-RF16-group, Bacteroidales_BS11_gut_group, Prevotellaceae, and Rikenellaceae_RC9_gut_group were lesser (p < 0.05) with supplementary concentrate feed (C and RPA+C) than without concentrate feed (CON and RPA), whereas Butyrivibrio_2 and Pseudobutyrivibrio were greater (p < 0.05) with supplementary rumen-protected amino acids (RPA and RPA+C) than without rumen-protected amino acids (CON and C). These results demonstrate that supplementary feed: (1) alters the composition of rumen microbiota and concentrations of ruminal VFAs in lactating yaks; and (2) can be used to manipulate the composition of rumen microbiota.

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

Roux-en-Y Gastrointestinal Bypass Promotes Activation of TGR5 and Peptide YY

2020 ◽  
Vol 20 (8) ◽  
pp. 1262-1267
Author(s):  
Haojun Yang ◽  
Hanyang Liu ◽  
YuWen Jiao ◽  
Jun Qian
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Metabolic Diseases ◽  
Peptide Yy ◽  
The Body ◽  
Wild Type ◽  
L Cells ◽  
Body Weights ◽  
Gastrointestinal Bypass ◽  
G Protein Coupled

Background: G protein-coupled bile acid receptor (TGR5) is involved in a number of metabolic diseases. The aim of this study was to identify the role of TGR5 after Roux-en-Y gastric bypass (GBP). Methods: Wild type and TGR5 knockout mice (tgr5-/-) were fed a high-fat diet (HFD) to establish the obesity model. GBP was performed. The changes in body weight and food intake were measured. The levels of TGR5 and peptide YY (PYY) were evaluated by RT-PCR, Western blot, and ELISA. Moreover, the L-cells were separated from wild type and tgr5-/- mice. The levels of PYY in L-cells were evaluated by ELISA. Results: The body weights were significantly decreased after GBP in wild type mice (p<0.05), but not tgr5-/- mice (p>0.05). Food intake was reduced after GBP in wild type mice, but also not significantly affected in tgr5-/- mice (p>0.05). The levels of PYY were significantly increased after GBP compared with the sham group (p<0.05); however, in tgr5-/- mice the expression of PYY was not significantly affected (p>0.05). After INT-777 stimulation in L-cells obtained from murine intestines, the levels of PYY were significantly increased in L-cells tgr5+/+ (p<0.05). Conclusion: Our study suggests that GBP up-regulated the expression of TGR5 in murine intestines, and increased the levels of PYY, which further reduced food intake and decreased the body weight.

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