scholarly journals CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1347
Author(s):  
Chao Zhu ◽  
Yue Jiang ◽  
Junru Zhu ◽  
Yonglong He ◽  
Hao Yin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Negative Feedback ◽  
Target Gene ◽  
Molecular Mechanisms ◽  
Mammary Epithelial Cells ◽  
Noncoding Rnas ◽  
Mammary Epithelial ◽  
Circular Rnas ◽  
Stanniocalcin 2 ◽  
Goat Mammary Epithelial Cells

Circular RNAs (circRNAs), which are considered a large class of endogenous noncoding RNAs, function as regulators in various biological procedures. In this study, the function and molecular mechanisms of circRNA8220 in goat mammary epithelial cells (GMECs) were explored. CircRNA8220 could spong miR-8516 and block the function of miR-8516 by binding to the target site of miR-8516 a negative feedback relationship existed between circRNA8220 and miR-8516. Stanniocalcin 2 (STC2) was a target gene of miR-8516. circRNA8220 could up-regulate the expression of STC2 by sponging miR-8516 in GMECs. circRNA8220/miR-8516/STC2 could promote proliferation and enhance the synthesis of β-casein and triglycerides (TG) via Ras/MEK/ERK and PI3K/AKT/mTOR signaling pathways, respectively.

ADIPOR1 regulates genes involved in milk fat metabolism in goat mammary epithelial cells

2021 ◽  
Author(s):  
Wangsheng Zhao ◽  
Michael Adjei ◽  
Hongmei Wang ◽  
Yueling Yangliu ◽  
Jiangjiang Zhu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Fat Metabolism ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells

Effect of Relaxin on Primary Cultures of Goat Mammary Epithelial Cells

1995 ◽  
pp. 87-88
Author(s):  
Sylvia Kondo ◽  
Colin J. Wilde ◽  
Gillian D. Bryant-Greenwood ◽  
Eleanor Taylor ◽  
Malcolm Peaker ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Primary Cultures ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells

scholarly journals PIN1 Is an E2F Target Gene Essential for Neu/Ras-Induced Transformation of Mammary Epithelial Cells

2002 ◽  
Vol 22 (15) ◽  
pp. 5281-5295 ◽  
Author(s):  
Akihide Ryo ◽  
Yih-Cherng Liou ◽  
Gerburg Wulf ◽  
Masafumi Nakamura ◽  
Sam W. Lee ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cyclin D1 ◽  
Transcriptional Activation ◽  
Target Gene ◽  
Mammary Epithelial Cells ◽  
Cell Transformation ◽  
Mammary Tumorigenesis ◽  
Mammary Epithelial ◽  
Oncogenic Signaling ◽  
And Function

ABSTRACT Oncogenes Neu/HER2/ErbB2 and Ras can induce mammary tumorigenesis via upregulation of cyclin D1. One major regulatory mechanism in these oncogenic signaling pathways is phosphorylation of serines or threonines preceding proline (pSer/Thr-Pro). Interestingly, the pSer/Thr-Pro motifs in proteins exist in two completely distinct cis and trans conformations, whose conversion is catalyzed specifically by the essential prolyl isomerase Pin1. By isomerizing pSer/Thr-Pro bonds, Pin1 can regulate the conformation and function of certain phosphorylated proteins. We have previously shown that Pin1 is overexpressed in breast tumors and positively regulates cyclin D1 by transcriptional activation and posttranslational stabilization. Moreover, in Pin1 knockout mice, mammary epithelial cells fail to undergo massive proliferation during pregnancy, as is the case in cyclin D1 null mice. These results indicate that Pin1 is upregulated in breast cancer and may be involved in mammary tumors. However, the mechanism of Pin1 overexpression in cancer and its significance in cell transformation remain largely unknown. Here we demonstrate that PIN1 expression is mediated by the transcription factor E2F and enhanced by c-Neu and Ha-Ras via E2F. Furthermore, overexpression of Pin1 not only confers transforming properties on mammary epithelial cells but also enhances the transformed phenotypes of Neu/Ras-transformed mammary epithelial cells. In contrast, inhibition of Pin1 suppresses Neu- and Ras-induced transformed phenotypes, which can be fully rescued by overexpression of a constitutively active cyclin D1 mutant that is refractory to the Pin1 inhibition. Thus, Pin1 is an E2F target gene that is essential for the Neu/Ras-induced transformation of mammary epithelial cells through activation of cyclin D1.

ΔFosB regulates Ca2+ release and proliferation of goat mammary epithelial cells

Gene ◽  
2014 ◽  
Vol 545 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Huiling Zheng ◽  
Hui Li ◽  
Lihui Li ◽  
Shaoyang Ma ◽  
Xuemei Liu
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells

scholarly journals Microarray analysis of RhoC-transformed mammary epithelial cells suggests molecular mechanisms of inflammatory breast cancer

10.1038/87212 ◽  
2001 ◽  
Vol 27 (S4) ◽  
pp. 73-73
Author(s):  
Sofia Merajver ◽  
Zhi-Fen Wu ◽  
Tammy Chang ◽  
Hamid Mirshahidi ◽  
Paul Meltzer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Microarray Analysis ◽  
Inflammatory Breast Cancer ◽  
Molecular Mechanisms ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial

5-Hydroxy-l-tryptophan Promotes the Milk Calcium Level via the miR-99a-3p/ATP2B1 Axis in Goat Mammary Epithelial Cells

2020 ◽  
Vol 68 (10) ◽  
pp. 3277-3285
Author(s):  
Shunxin Chen ◽  
Haiying Zhao ◽  
Xiaoru Yan ◽  
Zhifei Zhang ◽  
Kaizhao Hu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Calcium Level ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells
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