scholarly journals Dominant Yeast Community in Raw Sheep’s Milk and Potential Transfers of Yeast Species in Relation to Farming Practices

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 906 ◽  
Author(s):  
Álvaro Rafael Quintana ◽  
José Manuel Perea ◽  
Beatriz García-Béjar ◽  
Lorena Jiménez ◽  
Ana Garzón ◽  
...  
Keyword(s):  
Mixed Model ◽  
Yeast Species ◽  
Dairy Farm ◽  
Farming Practices ◽  
Milk Samples ◽  
Yeast Community ◽  
La Mancha ◽  
Milk Contamination ◽  
Contamination Routes

Yeasts are always present in any type of cheese, as well as in the factories where it is produced. However, the role of the yeast community in the cheese making process, as well as the routes of contamination used by yeast species to contaminate milk from the dairy farm environment, are not well known. The objectives of this study were to broaden the knowledge of the dominant yeast community in Manchega sheep’s milk and to assess the contamination routes of the yeast species depending on the farm practices. Milk, teat surface (collected from ten ewes per farm), feed, and air (collected in milking parlours and livestock housing) samples were collected from 12 typical farms in Castilla-La Mancha, Spain with differences in farming practices, and the yeast species were identified using DNA sequencing methods. To evaluate whether certain farming practices have an effect on the distribution of species of yeast in the milk samples, a mixed model was used. The results showed that most of the dominant yeast species (mainly belonging to the genus Candida) found in milk were also found in the other samples, indicating a microbial transfer from the farm environment to the milk. Furthermore, the statistical model showed that factors influencing yeast counts in milk were the presence of yeasts in the milking parlour, the use of silage, and the frequency of acid treatment for cleaning the milking machines. In conclusion, milk contamination from the yeast species present in the dairy farm environment is related to certain farming practices such as the use of silage and the daily use of acid in the cleaning of the milking machines, which favours the presence of desirable microbiota in milk.

scholarly journals Influence of Environmental and Productive Factors on the Biodiversity of Lactic Acid Bacteria Population from Sheep Milk

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2180
Author(s):  
Álvaro Rafael Quintana ◽  
José Manuel Perea ◽  
María Llanos Palop ◽  
Ana Garzón ◽  
Ramón Arias
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Mixed Model ◽  
Animal Feed ◽  
Dairy Industry ◽  
Dairy Farm ◽  
Sheep Milk ◽  
La Mancha ◽  
Milking Machine

Milk is a typical and satisfactory medium for the growth of lactic acid bacteria (LAB). These microorganisms are of vital importance in the quality of the milk since they contribute to its preservation and give differential organoleptic properties to the final product. Furthermore, LABs can act as biocontrol agents in the dairy industry by inhibiting the growth of undesirable bacteria present in milk and by improving the quality of dairy products such as cheese. In this context, knowing the transfer routes used by LABs from the livestock environment to the milk is of great importance within the dairy industry. Therefore, the objectives of the present study were to expand the knowledge of the LAB population present in the milk of Manchego ewe by means of DNA sequencing techniques and to evaluate the possible transfers of LAB species based on the management of each dairy farm. Samples of bulk tank milk, air (from the milking parlour and from the livestock housing), animal feed and teat surface (taken from 10 sheep per farm) were collected in 12 traditional livestock farms in Castilla-La Mancha (Spain), where each farm presented differences regarding their farming practices. A mixed-effects model was used to evaluate the effects of livestock practices on the distribution of LAB species. Results showed that the vast majority of species identified in the milk had an isolate that was also found in other matrices, which could indicate a microbial transference via the livestock environment to the milk. In addition, the mixed model showed that the factors that positively influence the LAB count were the low-line milking system and the daily use of acid detergent in cleaning the milking machine.

scholarly journals Influence of Microencapsulation on Fermentative Behavior of Hanseniaspora osmophila in Wine Mixed Starter Fermentation

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 112
Author(s):  
Grazia Alberico ◽  
Angela Capece ◽  
Gianluigi Mauriello ◽  
Rocchina Pietrafesa ◽  
Gabriella Siesto ◽  
...  
Keyword(s):  
Yeast Species ◽  
Starter Culture ◽  
Cell Immobilization ◽  
Mixed Fermentation ◽  
Wine Composition ◽  
Saccharomyces Yeast ◽  
Suitable System ◽  
Saccharomyces Yeasts ◽  
Fermentative Process

In recent years, as a consequence of the re-evaluation of the role of non-Saccharomyces yeasts, several studies have been conducted on the use of controlled mixed fermentations with Saccharomyces and different non-Saccharomyces yeast species from the winemaking environment. To benefit from the metabolic particularities of some non-Saccharomyces yeasts, the management of a non-Saccharomyces strain in mixed fermentation is a crucial step, in particular the use of procedures addressed to increase the persistence of non-Saccharomyces strains during the fermentative process. The use of microencapsulation for cell immobilization might represent a strategy for enhancing the competitiveness of non-Saccharomyces yeasts during mixed fermentation. This study was aimed to assess the fermentative performance of a mixed starter culture, composed by a wild Hanseniaspora osmophila strain (ND1) and a commercial Saccharomyces cerevisiae strain (EC1118). For this purpose, free and microencapsulated cells of ND1 strain were tested in co-culture with EC1118 during mixed fermentations in order to evaluate the effect of the microencapsulation on fermentative behavior of mixed starter and final wine composition. The data have shown that H. osmophila cell formulation affects the persistence of both ND1 and EC1118 strains during fermentations and microencapsulation resulted in a suitable system to increase the fermentative efficiency of ND1 strain during mixed starter fermentation.

The role of information structure in the instantiation of objects: Evidence from Amazonian Spanish

2020 ◽  
Vol 13 (1) ◽  
pp. 219-245
Author(s):  
Rosa Vallejos ◽  
Evelyn Fernández-Lizárraga ◽  
Haley Patterson
Keyword(s):  
Regression Analysis ◽  
Information Structure ◽  
Mixed Model ◽  
Linguistic Variables ◽  
Social Variables ◽  
Null Objects ◽  
The Impact ◽  
Role Of Information ◽  
Activation Status

AbstractThis study analyzes the instantiation of objects in Peruvian Amazonian Spanish (PAS) discourse in two communities with distinct linguistic contexts. We examine the impact of two social variables (gender and place) and nine linguistic variables (transitivity, animacy, definiteness, anaphora function, anaphora expression, cataphora function, cataphora expression, activation, topic persistence) on the speech of eight participants. Our findings indicate that null instantiation in PAS is pervasive, occurring with a range of verb lexemes. While neither gender nor place are significant predictors of null objects, various linguistic variables contribute to the instantiation of objects. The five significant variables as determined by a mixed model regression analysis include the following: animacy, definiteness, anaphora expression, cataphora expression, and activation status. Several findings are consistent with previous research (e. g. human and definite referents disfavor null objects), while other results differ (e. g. PAS propositions disfavor null objects). Activation status and anaphora expression are the most significant predictors of null objects in PAS. In particular, highly accessible referents in discourse and anaphoric null objects favored null objects in subsequent clauses. Thus, the results in the present study demonstrate the pivotal role of information structure in object instantiation, furthering the discussion on syntax-discourse interplay phenomena.

Don Quixote, Part I (1605)

2021 ◽  
pp. 86-117
Author(s):  
Edwin Williamson
Keyword(s):  
Don Quixote ◽  
Cultural Significance ◽  
Second Best ◽  
La Mancha ◽  
The World ◽  
Insight Into

When it first appeared in 1605, Cervantes’s great novel Don Quixote de la Mancha became an international sensation. This chapter follows the chronology of the plot in a critical manner providing the reader with important insight into why Don Quixote has become the second best-selling book of all time. Including the famous scenes of the windmill, the liquidation of Don Quixote’s library, the funeral of Grisóstomo and redemption of Marcela, Ginés de Pasamonte and the galley slaves, El curioso impertinente, the famous tale of Cardenio the star-crossed lover, and a commentary on the role of drama and novels of chivalry in society, Don Quixote, Part One sets the hero and his trusty country squire against the world at large. This chapter explores its deep cultural significance and answers the question of whether or not it is merely a ‘funny book’.

scholarly journals The Potential Role of Direct and Indirect Contacts on Infection Spread in Dairy Farm Networks

2017 ◽  
Vol 13 (1) ◽  
pp. e1005301 ◽  
Author(s):  
Gianluigi Rossi ◽  
Giulio A. De Leo ◽  
Stefano Pongolini ◽  
Silvano Natalini ◽  
Luca Zarenghi ◽  
...  
Keyword(s):  
Potential Role ◽  
Dairy Farm ◽  
Infection Spread

scholarly journals What makes human–animal relations ‘organizational’? The de-scription of anthrozootechnical agencements

Organization ◽  
2016 ◽  
Vol 24 (6) ◽  
pp. 761-780 ◽  
Author(s):  
Antoine Doré ◽  
Jérôme Michalon
Keyword(s):  
Network Theory ◽  
Performance Test ◽  
Dairy Farm ◽  
Actor Network Theory ◽  
Analytical Framework ◽  
Organization Studies ◽  
Relational Properties ◽  
Course Of Action ◽  
Human Animal

Questions concerning animals’ role in society have received little attention from Organization Studies. This article develops and tests some theoretical and methodological propositions aimed at contributing to the elaboration of an analytical framework for interpreting our organized relations with animals and furthering our understanding of what makes human–animal relations ‘organizational’. First, examining the role of animals in the ‘non-human turn’ that has been emerging, especially with the Actor–Network Theory and the Symmetrical Anthropology project, it adresses the limits of the ‘non-human’ category to analyze situations of coordination of collective action involving animals. It then develops the concept of anthrozootechnical agencement to envisage the role of animals in the course of action through the lens of their relational properties and applies the notion of script to propose an operational formulation of the specifically organizational trials to which these particular agencements are subjected. Based on three case studies (the role of the leash in the organization of human–dog relations, the management of wolves’ return to France, and the production of milk on a dairy farm), this article shows that two main types of operation make human–animal relations ‘organizational’: first, the organization of anthrozootechnical relations is constituted by and constitutive of the combination of three types of specifically organizational test to which these particular agencements are subjected (the performance test, the coherence test, and the dimensioning test); second, the work of organizing anthrozootechnical relations then consists in elaborating, executing, and transforming heterogeneous scripts that are never strictly indexed on the nature (human, animal, technique) of the entities they concern.

scholarly journals The presence of pathogens in milk of ewes in relation to the somatic cell count and subpopulations of leukocytes

2021 ◽  
Author(s):  
Tvarožková ◽  
Vašíček ◽  
Uhrinčať ◽  
Mačuhová ◽  
Hleba ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Polymorphonuclear Cells ◽  
Coagulase Negative Staphylococci ◽  
Major Health Problem ◽  
Milk Samples

Mastitis is a major health problem of the udder in dairy sheep breeds. For diagnosis of subclinical mastitis, somatic cell count (SCC) is commonly used. The presence of pathogens in the udder causes the increase of leukocytes and thus SCC in milk. Therefore, the aim of this study was to evaluate the presence of pathogens in the milk of ewes and the possible relationship with SCC. The changes of leukocytes subpopulation in milk samples with high SCC were evaluated as well. The experiment was carried out on a dairy farm with the Lacaune breed. This study was conducted on 45 ewes (98 milk samples) without signs of clinical mastitis. Based on somatic cell count, samples were divided to five SCC groups: SCC1 &lt; 200 000 cells/ml (45 milk samples); 200 000 ≤ SCC2 &lt; 400 000 cells/ml (10 milk samples); 400 000 ≤ SCC3 &lt; 600 000 cells/ml (six milk samples); 600 000 ≤ SCC4 &lt; 1 000 000 cells/ml (six milk samples); SCC5 ≥ 1 000 000 cells/ml (31 milk samples). No pathogens were observed in the majority of milk samples (60.20%). Coagulase-negative staphylococci (CNS) were the most commonly isolated pathogens from the milk of ewes (86.11%). Staphylococcus epidermidis had the highest incidence from CNS (35.48%). In the SCC5 group, up to 79.31% of bacteriological samples were positive. The percentage of leukocytes significantly increased (P &lt; 0.001) in the samples with higher SCC (≥ 200 × 10<sup>3</sup> cells/ml) in comparison to the group SCC1. Also, the percentage of polymorphonuclear cells (PMNs) was significantly higher with increasing SCC (P &lt; 0.001). In conclusion, the presented results showed that the high SCC was caused by the presence of the pathogen in milk. Thus SCC &lt; 200 000 cells/ml and leukocyte subpopulation, especially PMNs, could be considered as important tools in udder health programs applied in dairy ewes.

The Potential for Sustainable Organic Farming in Nagaland: A Case Study

2022 ◽  
pp. 101852912110652
Author(s):  
Devpriya Sarkar
Keyword(s):  
Organic Farming ◽  
Crop Residues ◽  
The State ◽  
Cattle Dung ◽  
Farming Practices ◽  
Eastern State ◽  
North Eastern ◽  
Traditional Mode

In 2015, Sikkim, a North-Eastern state of India, achieved the state of being fully organic. Later, states like Nagaland, Mizoram, Meghalaya, Goa and Kerala have declared their intentions to be fully organic. In Nagaland, more than 47% of the population is engaged in agriculture and entirely dependent on the traditional mode of farming and has used organic manure like cattle dung, dried leaves-litter and crop residues for enhancing the capacity of soil from time immemorial. Also, studies have shown that the state of Nagaland has negligible use of inorganic supplements in their fields. Thus, Nagaland has a high potential to be converted into an organic state without making any significant shifts in their existing farming practices. Shifting cultivation, locally known as Jhum-kheti, is one of the oldest forms of the agricultural process in practice in Nagaland. However, some studies regard Jhum cultivation as harmful to the environment, but there is a scope to reinvent this farming method and move towards a more sustainable form of agriculture there. This study explores the relation between traditional farming and organic farming and the benefits of state-induced organic farming methods and their effects on the farmers of Nagaland. A survey was carried out in the Mokokchung district of Nagaland to understand the role of farmers in attaining sustainability.

Impacto de la pandemia por COVID19 en la percepción de competencia motriz de escolares de la ciudad de Temuco, Chile (Impact of the COVID19 pandemic on the perception of motor competence in schoolchildren from Temuco, Chile)

Retos ◽  
2021 ◽  
Vol 43 ◽  
pp. 361-369
Author(s):  
Jaime Carcamo-Oyarzun ◽  
Francisco Romero-Rojas ◽  
Isaac Estevan
Keyword(s):  
Physical Education ◽  
Motor Development ◽  
Mixed Model ◽  
Physical Education Class ◽  
Motor Competence ◽  
Object Control ◽  
Face To Face ◽  
Underlying Mechanisms ◽  
Didactic Strategies

  En el ámbito del desarrollo motor, la percepción de la competencia motriz es considerada como uno de los mecanismos subyacentes más importantes que influyen en la adherencia a la práctica de actividad física, siendo las clases de Educación Física una de las instancias más pertinentes para su desarrollo. El presente estudio tiene como objetivo analizar, en condiciones de confinamiento y de cierre de las escuelas por COVID-19, cómo la percepción de la competencia motriz evoluciona durante el tiempo en que el alumnado no ha tenido Educación Física de manera presencial. Participaron 104 estudiantes (52,9% niñas; M = 10,9 años de edad; D.T. = 0,69), quienes contestaron el cuestionario SEMOK de evaluación de la competencia motriz percibida, tanto en 2019 (T1) como en 2020 (T2). Los resultados del modelo mixto no mostraron un efecto principal del periodo de confinamiento sobre la percepción de competencia motriz (p > ,05), mientras que en cada una de los tiempos de medición se encontraron diferencias significativas en función del sexo, donde los niños mostraron una mayor percepción de competencia motriz en control de objetos que las niñas (p < ,001). Los estudiantes mantuvieron una percepción similar desde T1 hasta T2, mostrando una estabilidad en la percepción de su competencia motriz durante este periodo de confinamiento, lo que coincide con los escasos estudios que han investigado esta temática siguiendo procedimientos prospectivos. Además, se confirma el rol que el sexo ejerce en la percepción de la competencia motriz, aspecto que debe ser considerado al momento de desarrollar estrategias didácticas en Educación Física.  Abstract. In the field of motor development, the perception of motor competence is considered one of the most important underlying mechanisms that influence the adherence to the practice of physical activity, where Physical Education classes are one of the most relevant instances for its development. The present study aims to analyze how the perception of motor competence evolves in conditions of confinement and closed schools because of COVID-19 during the time in which the students have not had Physical Education classes in a face-to-face manner. 104 students (52.9% girls; age M=10.9; S.D.=0.69) participated and answered the SEMOK questionnaire for assessing perceived motor competence in both 2019 (T1) and 2020 (T2). The results of the mixed model did not present major effects of the confinement period on the perception of motor competence (p>.05), while in each of the periods significant differences were found according to gender, with boys showing a higher perception of motor competence in object control than girls (p<.001). The students maintained a similar perception from T1 to T2, showing stability in the perception of their motor competence during the period of confinement, which coincides with the few studies that have studied this subject, following prospective procedures. In addition, the role of gender in the perception of motor competence is confirmed and should be considered when developing didactic strategies for Physical Education class.

scholarly journals Lifetime productivity and repeatability estimation of selected traits of crossbred cows in Savar Dairy Farm

2018 ◽  
Vol 29 (2) ◽  
pp. 168-177
Author(s):  
MS Islam ◽  
A Akhtar ◽  
MA Hossain ◽  
MF Rahman ◽  
SS Hossain
Keyword(s):  
Milk Yield ◽  
Mixed Model ◽  
Dairy Farm ◽  
Cattle Breeding ◽  
Crossbred Cows ◽  
Lactation Length ◽  
Genetic Groups ◽  
Age At Puberty ◽  
Selected Traits ◽  
Productive Traits

The study was conducted on 244 crossbred cows of (Central Cattle Breeding and Diry Farm), Savar, Dhaka to evaluate the effect of different productive groups on their lifetime performance of various productive traits using twenty years data.  Data were accumulated from a prescribed data sheet maintained by Central Cattle Breeding Station, Savar, Dhaka. Genetic groups for this experiment were Local Friesian (LF), Local Jersey (LJ), Local Hariana X Friesian (LH x F), Local Friesian X Local Friesian (LF x LF) and Local Jersey X Local Jersey (LJ x LJ). The studied parameters were age at puberty, lactation length, lactation yield, total milking days, and total milk yield in lifetime. The data were analyzed using Least-Squares Mixed Model and Maximum Likelihood Computer Program (Harvey, 1990). Analysis showed that genetic groups had a significant effect on age at puberty, lactation length and lactation yield (P<0.01), total milking days and milk yield in lifetime (P<0.001). From the result it was found that earliest age at puberty were in L x F (749.27±99.01). Lactation length and standardized lactation yield were also higher in L x F (258.69±20.04 days and 1408.80±83.60 kg respectively). Total milking days and milk yield were observed higher in (LH x F) with mean of 1468.30±198.58 days and 7340.32±1813.28 kg, respectively. The repeatability estimates of lactation length for genetic groups LF, LJ, LH x F, LF x LF and LJ x LJ were 0.24±0.09, 0.04(-ve), 0.10±0.05, 0.07±0.05 and 0.49±0.22, respectively. The same for lactation yield was 0.17±0.08, 0.27± 0.02, 0.07±0.04, 0.17±0.08 and 0.03±0.05 respectively. From analysis it was revealed that in respect of productive performances the L x F genetic group was found superior compared to other groups but interns of total lifetime productivity, LH x F ranked the highest in the given environment. It is concluded that lifetime productivity needs to be considered as an appropriate guideline for selecting genetic groups for future productivity.Progressive Agriculture 29 (2): 168-177, 2018

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