scholarly journals An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 730
Author(s):  
Anna Perazzi ◽  
Chiara Gomiero ◽  
Livio Corain ◽  
Ilaria Iacopetti ◽  
Enrico Grisan ◽  
...  
Keyword(s):  
Organ Culture ◽  
Diagnostic Methods ◽  
Assay System ◽  
Automated Image Analysis ◽  
Ocular Structure ◽  
Culture Model ◽  
Significant Difference ◽  
Corneal Organ Culture ◽  
Structure Investigations

The purpose of this study was to investigate the response of porcine corneal organ cultures to riboflavin/UV-A phototherapy in the injury healing of induced lesions. A porcine corneal organ culture model was established. Corneal alterations in the stroma were evaluated using an assay system, based on an automated image analysis method able to (i) localize the holes and gaps within the stroma and (ii) measure the brightness values in these patches. The analysis has been performed by dividing the corneal section in 24 regions of interest (ROIs) and integrating the data analysis with a “multi-aspect approach.” Three group of corneas were analyzed: healthy, injured, and injured-and-treated. Our study revealed a significant effect of the riboflavin/UV-A phototherapy in the injury healing of porcine corneas after induced lesions. The injured corneas had significant differences of brightness values in comparison to treated (p < 0.00) and healthy (p < 0.001) corneas, whereas the treated and healthy corneas showed no significant difference (p = 0.995). Riboflavin/UV-A phototherapy shows a significant effect in restoring the brightness values of damaged corneas to the values of healthy corneas, suggesting treatment restores the injury healing of corneas after lesions. Our assay system may be compared to clinical diagnostic methods, such as optical coherence tomography (OCT) imaging, for in vivo damaged ocular structure investigations.

An organ culture model for study of biochemical development of fetal rat lung

1978 ◽  
Vol 45 (3) ◽  
pp. 355-362 ◽  
Author(s):  
I. Gross ◽  
G. J. Smith ◽  
W. M. Maniscalco ◽  
M. R. Czajka ◽  
C. M. Wilson ◽  
...  
Keyword(s):  
Organ Culture ◽  
Late Gestation ◽  
In Vivo Studies ◽  
Morphological Development ◽  
Rat Lung ◽  
Fetal Rat ◽  
Culture Model ◽  
Fetal Rat Lung

We have developed a short-term organ culture model for the study of the biochemical and morphological development of late gestation fetal rat lung. Explants (1 mm3) of 19-day lung were cultured in an oxygen enriched environment in the presence of synthetic serum-free medium for 3 days. Morphological maturation continued in culture. The rate of incorporation of choline into disaturated phosphatidylcholine and the content of this phospholipid in the explants increased in vitro in a pattern very similar to that which occurs in vivo. The activities of choline kinase and cholinephosphotransferase were also similar in cultured lung and in vivo. Studies of glucose oxidation to CO2 provided additional evidence that the explants remained viable in culture. The explants retained the sensitivity of fetal lung to hormonal action. This was demonstrated by the stimulation of choline incorporation into phospholipid by cyclic AMP and an increase in the glycogen content after exposure to insulin.

scholarly journals Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

10.3791/58562 ◽  
2019 ◽  
Author(s):  
Nileyma Castro ◽  
Stephanie R. Gillespie ◽  
Audrey M. Bernstein
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Ex Vivo ◽  
Culture Model ◽  
Corneal Organ Culture

Skin Organ Culture as an Alternative to In Vivo Dermatotoxicity Testing

1993 ◽  
Vol 21 (4) ◽  
pp. 443-449
Author(s):  
Johannes J.M. van de Sandt ◽  
Jacqueline van Schoonhoven ◽  
Wilfred J.M. Maas ◽  
Alphons A.J.J.L. Rutten
Keyword(s):  
Organ Culture ◽  
Chloroacetic Acid ◽  
Tetrazolium Salt ◽  
Dependent Manner ◽  
Cutaneous Toxicity ◽  
Culture Model ◽  
The Relationship ◽  
Dose Dependent ◽  
Skin Organ Culture

Various aspects of acute cutaneous toxicity were studied in a skin organ culture model. Chemicals were applied topically for four hours, after which cytotoxicity was assessed by measuring the conversion of the tetrazolium salt, MTT. The relationship between pKa and cytotoxicity was investigated for a homologous series of benzoic acids. In this series, salicylic acid had the lowest pKa and proved to be the most toxic compound. Furthermore, the pH of the carrier solution was shown to influence the toxicity of chloroacetic acid and acetic acid in a different way. Using skin discs of both human and rabbit origin, we found that human skin was more resistant to toxicity induced by the irritants benzalkonium chloride and formaldehyde. As an additional aspect of dermal toxicology, the percutaneous absorption of testosterone was studied. After topical application to rabbit skin discs, testosterone was absorbed in a dose-dependent manner and concurrent metabolism was demonstrated.

scholarly journals Establishment and Characterization of an Air-Liquid Canine Corneal Organ Culture Model To Study Acute Herpes Keratitis

2014 ◽  
Vol 88 (23) ◽  
pp. 13669-13677 ◽  
Author(s):  
R. M. Harman ◽  
L. Bussche ◽  
E. C. Ledbetter ◽  
G. R. Van de Walle
Keyword(s):  
Organ Culture ◽  
Culture Model ◽  
Herpes Keratitis ◽  
Corneal Organ Culture

scholarly journals Establishment of a Robust and Simple Corneal Organ Culture Model to Monitor Wound Healing

2021 ◽  
Vol 10 (16) ◽  
pp. 3486
Author(s):  
Sandra Schumann ◽  
Eva Dietrich ◽  
Charli Kruse ◽  
Salvatore Grisanti ◽  
Mahdy Ranjbar
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Mitomycin C ◽  
Ex Vivo ◽  
Corneal Tissue ◽  
Dependent Manner ◽  
Toxicological Studies ◽  
Culture Model ◽  
The Impact

The use of in vitro systems to investigate the process of corneal wound healing offers the opportunity to reduce animal pain inflicted during in vivo experimentation. This study aimed to establish an easy-to-handle ex vivo organ culture model with porcine corneas for the evaluation and modulation of epithelial wound healing. Cultured free-floating cornea disks with a punch defect were observed by stereomicroscopic photo documentation. We analysed the effects of different cell culture media and investigated the impact of different wound sizes as well as the role of the limbus. Modulation of the wound healing process was carried out with the cytostatic agent Mitomycin C. The wound area calculation revealed that after three days over 90% of the lesion was healed. As analysed with TUNEL and lactate dehydrogenase assay, the culture conditions were cell protecting and preserved the viability of the corneal tissue. Wound healing rates differ dependent on the culture medium used. Mitomycin C hampered wound healing in a concentration-dependent manner. The porcine cornea ex vivo culture ideally mimics the in vivo situation and allows investigations of cellular behaviour in the course of wound healing. The effect of substances can be studied, as we have documented for a mitosis inhibitor. This model might aid in toxicological studies as well as in the evaluation of drug efficacy and could offer a platform for therapeutic approaches based on regenerative medicine.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

A Comparative Ex Vivo Study of Plasminogen Activators and Proteases for Fibrinolytic Activity and Side Effects in Rabbits

1977 ◽  
Vol 37 (01) ◽  
pp. 154-161 ◽  
Author(s):  
B. A Janik ◽  
S. E Papaioannou
Keyword(s):  
Side Effects ◽  
Effective Dose ◽  
Fibrinolytic Activity ◽  
Ex Vivo ◽  
Plasminogen Activators ◽  
Assay System ◽  
Coagulation Parameters ◽  
Ex Vivo Assay

SummaryUrokinase, streptokinase, Brinase, trypsin, and SN 687, a bacterial exoprotease, have been evaluated in an ex vivo assay system. These enzymes were injected into rabbits and the fibrinolytic activity as well as other coagulation parameters were measured by in vitro techniques. Dose-response correlations have been made using the euglobulin lysis time as a measure of fibrinolytic activity and the 50% effective dose has been determined for each enzyme. Loading doses, equal to four times the 50% effective dose, were administered to monitor potential toxicity revealing that Brinase, trypsin, and SN 687 were very toxic at this concentration.Having established the 50% effective dose for each enzyme, further testing was conducted where relevant fibrinolytic and coagulation parameters were measured for up to two days following a 50% effective dose bolus injection of each enzyme. Our results have demonstrated that urokinase and streptokinase are plasminogen activators specifically activating the rabbit fibrinolytic system while Brinase, trypsin and SN 687 increase the general proteolytic activity in vivo.The advantages of this ex vivo assay system for evaluating relative fibrinolytic potencies and side effects for plasminogen activators and fibrinolytic proteases have been discussed.

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