scholarly journals Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 601 ◽  
Author(s):  
Simone Ceccobelli ◽  
Emiliano Lasagna ◽  
Eymen Demir ◽  
Giacomo Rovelli ◽  
Emidio Albertini ◽  
...  
Keyword(s):  
Information Content ◽  
Polymorphic Information Content ◽  
Low Cost ◽  
Local Population ◽  
Geographic Area ◽  
Factorial Correspondence Analysis ◽  
Umbria Region ◽  
Goat Population ◽  
Components Analysis ◽  
Microsatellite Alleles

Italy holds important genetic resources of small ruminant breeds. By distinguishing goat breeds at the DNA level, certification of products from specific breeds can be valorized. The aim of this study was to establish the genetic identity of Facciuta della Valnerina, a local goat population of Italy, compared with the cosmopolitan breeds, Saanen and Camosciata delle Alpi, reared in the same geographic area. A total of 116 microsatellite alleles ranging from 4 to 13 were detected at 16 loci in the three goat populations/breeds. A total of 23 private alleles with frequencies lower than 0.3 were detected in the Facciuta della Valnerina population. The mean numbers of alleles were 6.67, 4.58, and 4.92 in Facciuta della Valnerina, Camosciata delle Alpi, and Saanen, respectively. The expected heterozygosity ranged from 0.20 to 0.86. Most loci were highly polymorphic and informative (polymorphic information content ≥0.50). Factorial correspondence analysis and principal components analysis revealed very clear separation between Facciuta della Valnerina and the two reference goat breeds. Reducing the number of markers from 16 to 12 (on the basis of polymorphic information content and the number of alleles) still allowed us to distinguish the local population, indicating that microsatellite markers are capable of discriminating local livestock breeds at a low cost.

scholarly journals Genetic Diversity Analysis in Chickpea (Cicer arietinum L) Genotypes Grown in Northwestern Algeria using Microsatellite Markers (SSR)

2019 ◽  
Author(s):  
Bouri Amina ◽  
Mediouni Mohammed Rida ◽  
Ameur Ameur Abdelkader ◽  
Udupa Sripada ◽  
Gaouar Souheil Bechir Semir
Keyword(s):  
Genetic Diversity ◽  
Information Content ◽  
Cicer Arietinum ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Geographic Origin ◽  
Diversity Analysis ◽  
Factorial Correspondence Analysis ◽  
Genetic Diversity Analysis ◽  
Cicer Arietinum L

The present study aimed to characterize a subset of 10 selected chickpea accessions (Cicer arietinum L.) using SSR. The result indicated a presence of a total of 59 alleles. The genetic diversity at the 15 microsatellites loci was varied from 0, 32 for TA22 to 0.78 for TA72 and TA117 with an average of 0.66. Polymorphic information content (PIC) values ranged from 0.27 to 0.74. This study also detected a high significant (P less than 0.01) positive correlation between alleles per locus, gene diversity (H) and polymorphism information content (PIC). In the dendrogram and on the PCoA bi-plots, chickpea genotypes were adjoined according to their geographic origin, type of chickpea (Kabuli/ Desi). Nevertheless, the distribution of the different grouping through the factorial correspondence analysis (AFC) is due to the genetic variability.

scholarly journals Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Workia Ahmed ◽  
Tileye Feyissa ◽  
Kassahun Tesfaye ◽  
Sumaira Farrakh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Information Content ◽  
Date Palm ◽  
Polymorphic Information Content ◽  
Phoenix Dactylifera ◽  
Mean Value ◽  
Date Palms ◽  
Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

scholarly journals Genotyping oil flax varieties using the microsatellite DNA marker system

2020 ◽  
Vol 21 (5) ◽  
pp. 531-539
Author(s):  
S. Z. Guchetl ◽  
T. A. Tchelyustnikova
Keyword(s):  
Information Content ◽  
Genetic Relatedness ◽  
Polymorphic Information Content ◽  
Biotic And Abiotic Stresses ◽  
Crop Acreage ◽  
Marker System ◽  
Average Value ◽  
Ssr Loci ◽  
New Varieties ◽  
Microsatellite Dna Marker

The tendency to increase crop acreage of oil flax requires the development of new varieties with high indicators of ecological plasticity, productivity and resistance to biotic and abiotic stresses. The application of modern biotechnolog ical approaches based on the use of molecular markers can accelerate the assessment of genetic differences and the dete rmination of potential of the source material for breeding. The research was aimed at assessment of the genotyping parameters of some oil flax varieties of VNIIMK breeding using the system of microsatellite markers. Seventeen variety samples of flax were used as the material for the research. DNA was isolated using CTAB buffer. Eleven SSR loci were used for the identification of varieties. Ten polymorphic loci were identified during the research. The total number of counted alleles is 32. The size of alleles varied in the range of 111-210 bps. The number of alleles per locus ranged from 2 to 6 with an average value of 3.20. The value of the index of polymorphic information content (PIC) was from 0.29 to 0.75 with an average parameter value of 0.51. The effective number of alleles for different loci is determined in the range of 1.40-3.94 with an average value of 2.28. The level of information content of the marker system (PIC 0.51) corresponds to that for identifying sets of genotypes from collections with a limitation in geographical origin. There were established differences in the frequency of occurrence of alleles. The discriminatory potential of the used marker system allowed to identify 15 variety samples. Two genotypes with common origin were identical. The degree of genetic relatedness of the studied flax genotypes has been evaluated. The obtained results will serve as the basis for the subsequent construction of genetic passports of oil flax varieties of VNIIMK breeding.

scholarly journals Serviço de apoio a distância ao professor em sala de aula pela tv digital interativa

2004 ◽  
Vol 1 (2) ◽  
pp. 53
Author(s):  
Sergio Ferreira do Amaral ◽  
Luciana Ozello Baratti ◽  
Daniel Moutinho Bataca ◽  
João Henrique de Augustinis Franco ◽  
José Manuel Martin Rios ◽  
...  
Keyword(s):  
New Technologies ◽  
Low Cost ◽  
Geographic Area ◽  
College Of Education ◽  
Digital Tv ◽  
Multimedia Content ◽  
Multimedia Material ◽  
Search Tool ◽  
Practical Development ◽  
Interactive Digital Tv

Este é um artigo de um projeto em andamento desenvolvido em parceria com a Diretoria Técnica de Serviços do CPqD Telecom & IT Solutions e a Faculdade de Educação da UNICAMP, que objetiva estabelecer um serviço de apoio a distância aos professores em sala de aula em 03 Escolas da região de Barão Geraldo em Campinas/ SP , para utilizar a TV Digital Interativa como ferramenta de busca e desenvolvimento de conteúdo multimídia interativo nas práticas pedagógicas. Fundamentalmente esta ferramenta será de fácil uso por utilizar como terminal de escolha e exibição um televisor colocado nas salas de aula das escolas e controlado por controle remoto. O professor poderá interagir com o conteúdo exibido no televisor de modo a obter uma programação não linear que se desenvolverá conforme o seu projeto pedagógico. As principais características do serviço são a facilidade de uso, fornecimento a distância de material multimídia interativo, baixo custo de implantação e grande área geográfica de cobertura, proporcionada pela disponibilidade quase total de sinal de televisão no território nacional. Este serviço é parte de um conjunto de serviços de teleducação que estão sendo desenvolvidos para o futuro Sistema de TV Digital terrestre que será implantado no Brasil. Palavras-chave: Educação a distância; TV digital interativa; Novas tecnologias aplicadas na educação; Tecnologia da informação e comunicação Abstract This is an article of a developed in progress project in would seem the Direction Technique of Services of the CPqD Telecom & IT Solutions and the College of Education of the UNICAMP, that objective in the distance to establish a support services to the teacher in classroom in 03 Schools of the region of Barão Geraldo in Campinas, SP, to use the Interactive Digital TV as a search tool and practical development of interactive multimedia content in the pedagogical practical ones. Basically this tool will be easy to use as a terminal of choice and exhibition a television set placed in the school’s classrooms and controlled for remote control. The teacher be able to interact with according to the content shown in the television set in order to get a non linear programme that will develop this pedagogical project. The main services characteristics are the easiness of use, supply of the interactive at distance multimedia material, low cost of implantation and covering of a great geographic area, for the almost total availability of signal of television in domestic territory. This service is part of a set of TV-education services that are being developed for the coming System of terrestrial Digital TV that will be implanted in Brazil. Key words: Distance Education; Interactive Digital TV; New Technologies applied in Education

scholarly journals Microsatellite development via next-generation sequencing in Acacia stenophylla (Fabaceae) and Duma florulenta (Polygonaceae): two ecologically important plant species of Australian dryland floodplains

2018 ◽  
Author(s):  
Bruce F Murray ◽  
Michael A Reid ◽  
Shu-Biao Wu
Keyword(s):  
Gene Flow ◽  
Next Generation Sequencing ◽  
Microsatellite Markers ◽  
Information Content ◽  
Polymorphic Information Content ◽  
Extreme Variability ◽  
Polymorphic Microsatellite ◽  
Generation Sequencing ◽  
Semi Arid

Duma florulenta and Acacia stenophylla are two ecologically important but understudied species that naturally occur on the floodplains and riverbanks of Australia’s arid and semi-arid river systems. This paper describes the discovery and characterization of 12 and 13 polymorphic microsatellite markers for D. florulenta and A. stenophylla respectively. The number of alleles per locus for D. florulenta ranged from 2-12 with an average of 6.1. Across all samples, observed and expected heterozygosities ranged from 0.026 to 0.784 and 0.026 to 0.824 respectively and mean polymorphic information content was equal to 0.453. For A. stenophylla, the number of alleles per locus ranged between 2 and 8 with an overall mean of 4.8. Across all samples, observed and expected heterozygosities ranged from 0.029 to 0.650 and 0.029 to 0.761 respectively and mean polymorphic information content was 0.388. The developed suites of 12 and 13 microsatellite markers for D. florulenta and A. stenophylla respectively provide opportunity for novel research into mechanisms of gene flow, dispersal and breeding system and how they operate under the extreme variability these species are exposed to in the environments in which they live.

scholarly journals Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Caroline Apolinário-Silva ◽  
Dhiego G. Ferreira ◽  
Analiza F. Cavenagh ◽  
Nícollas G. O. Aprígio ◽  
Bruno A. Galindo ◽  
...  
Keyword(s):  
Information Content ◽  
Microsatellite Loci ◽  
Polymorphic Information Content ◽  
Neotropical Fish ◽  
Genetic Studies ◽  
Cross Amplification ◽  
Abstract Data ◽  
Polymorphic Microsatellite

ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species.

Intrabreed polymorphic information content of microsatellites in cattle and sheep

1998 ◽  
Vol 78 (3) ◽  
pp. 425-428 ◽  
Author(s):  
Fiona C. Buchanan ◽  
Tracey D. Thue
Keyword(s):  
Key Words ◽  
Information Content ◽  
Polymorphic Information Content ◽  
Livestock Species ◽  
The Common ◽  
Cattle And Sheep

Eight ovine and eleven bovine microsatellites were amplified in unrelated individuals from five breeds of sheep and cattle respectively. Polymorphic information content (PIC) was calculated for each breed. It was shown that the common practice of using genotypes from several breeds to calculate PIC for a livestock species can give misleading PIC values for a given breed. Key words: Microsatellite, polymorphic information content

scholarly journals Application of the ISSR method to estimate the genetic similarity of Dasypyrum villosum (L.) P. Candargy Greek populations to Triticum and Secale species

2011 ◽  
Vol 21 (1) ◽  
pp. 7-12
Author(s):  
Agnieszka Grądzielewska
Keyword(s):  
Information Content ◽  
Genetic Similarity ◽  
Polymorphic Information Content ◽  
Similarity Index ◽  
Issr Markers ◽  
Mean Value ◽  
Dasypyrum Villosum ◽  
Reproducible Method ◽  
The Mean ◽  
High Diversity

Application of the ISSR method to estimate the genetic similarity of Dasypyrum villosum (L.) P. Candargy Greek populations to Triticum and Secale species In the study, the genetic similarity between Dasypyrum villosum L. (P.) Candargy and Triticum L. and Secale L. species was studied on the basis of ISSR markers. As a very polymorphic, effective and reproducible method, ISSR can be successfully employed to evaluate polymorphism between and inside different species. The polymorphic information content values (PIC) of ISSR method ranged from 0.57 to 0.87, with the mean value of 0.7. The genetic similarity of the forms analyzed ranged from 0.27 to 0.97, with the mean value of 0.47, indicating their high diversity. A higher similarity of Dasypyrum villosum to Triticum species, in comparison with Secale was found - the mean Dice genetic similarity index between genera was calculated at 0.40 for Dasypyrum and Triticum, and at 0.31 for Dasypyrum and Secale.

scholarly journals PICcalc: An Online Program to Calculate Polymorphic Information Content for Molecular Genetic Studies

2012 ◽  
Vol 50 (9-10) ◽  
pp. 670-672 ◽  
Author(s):  
Sándor Nagy ◽  
Péter Poczai ◽  
István Cernák ◽  
Ahmad Mousapour Gorji ◽  
Géza Hegedűs ◽  
...  
Keyword(s):  
Information Content ◽  
Polymorphic Information Content ◽  
Molecular Genetic ◽  
Online Program ◽  
Genetic Studies
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