scholarly journals Characterization of Sheep Milk Extracellular Vesicle-miRNA by Sequencing and Comparison with Cow Milk

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 331 ◽  
Author(s):  
Suyu Quan ◽  
Xuemei Nan ◽  
Kun Wang ◽  
Linshu Jiang ◽  
Junhu Yao ◽  
...  
Keyword(s):  
Small Rnas ◽  
Regulatory Element ◽  
Signal Transmission ◽  
Extracellular Vesicle ◽  
Ovis Aries ◽  
Cow Milk ◽  
Bioactive Components ◽  
Sheep Milk ◽  
Bioactive Component

Milk can mediate maternal-neonatal signal transmission by the bioactive component extracellular vesicles (EVs), which select specific types of miRNA to encapsulate. The miRNA profiling of sheep milk EVs was characterized by sequencing and compared with that of cow milk. Nanoparticle tracking analysis revealed that the concentration of sheep milk EVs was 1.3 ± 0.09 × 1012 particles/mL and the diameter was peaked at 131.2 ± 0.84 nm. Sheep milk EVs contained various small RNAs, including tRNA, Cis-regulatory element, rRNA, snRNA, other Rfam RNA, and miRNA, which held about 36% of all the small RNAs. In total, 84 types of miRNA were annotated with Ovis aries by miRBase (version 22.0) in sheep milk EVs, with 75 shared types of miRNAs in all samples. The miR-26a, miR-191, let-7f, let-7b and miR-10b were highly expressed both in cow and sheep milk EVs, and 14 sheep milk EV-miRNAs in the top 20, occupying 98% of the total expression, were immune-related. Although pathway analysis showed different potential functions of cow and sheep milk EV-miRNAs, there were still some shared points: lipid metabolism (phospholipase D, glycerophospholipid and glycosylphosphatidylinositol), calcium metabolism, and nerve conduction (axon guidance and synapse). This study provides reference for the bioactive components in the milk of different species.

Characterization of Haemanthus coccineus extract and its bioactive component: A promising and novel anti-inflammatory approach

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
S Fuchs ◽  
L Tzung-Harn Hsieh ◽  
W Saarberg ◽  
L Schaefer ◽  
CAJ Erdelmeier ◽  
...  
Keyword(s):  
Bioactive Component ◽  
Anti Inflammatory

Characterization of small RNAs in early zebrafish PGCs

2021 ◽  
Author(s):  
Xiaoyuan Zhang ◽  
Yiping Li ◽  
Yunbin Zhang ◽  
Shifeng Li
Keyword(s):  
Small Rnas

scholarly journals Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes

2006 ◽  
Vol 20 (13) ◽  
pp. 1732-1743 ◽  
Author(s):  
Toshiaki Watanabe ◽  
Atsushi Takeda ◽  
Tomoyuki Tsukiyama ◽  
Kazuyuki Mise ◽  
Tetsuro Okuno ◽  
...  
Keyword(s):  
Small Rnas ◽  
Identification And Characterization

Choosing sheep (Ovis aries) as animal model for temporomandibular joint research: Morphological, histological and biomechanical characterization of the joint disc

Morphologie ◽  
2016 ◽  
Vol 100 (331) ◽  
pp. 223-233 ◽  
Author(s):  
D.F. Angelo ◽  
P. Morouço ◽  
N. Alves ◽  
T. Viana ◽  
F. Santos ◽  
...  
Keyword(s):  
Animal Model ◽  
Temporomandibular Joint ◽  
Ovis Aries ◽  
Joint Research

scholarly journals Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

2014 ◽  
Vol 83 (10) ◽  
pp. S3-S8 ◽  
Author(s):  
Lenka Necidová ◽  
Šárka Bursová ◽  
Alena Skočková ◽  
Bohdana Janštová ◽  
Pavla Prachařová ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Storage Temperature ◽  
Goat Milk ◽  
Storage Conditions ◽  
Cow Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plate Method ◽  
Milk Samples ◽  
Sheep Milk ◽  
Enterotoxin Production

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Transfusion ◽  
2014 ◽  
Vol 55 (3) ◽  
pp. 507-521 ◽  
Author(s):  
Annika Pienimaeki-Roemer ◽  
Katja Kuhlmann ◽  
Alfred Böttcher ◽  
Tatiana Konovalova ◽  
Anne Black ◽  
...  
Keyword(s):  
Extracellular Vesicle

High-power InAlAs/InGaAs Schottky barrier photodiodes for analog microwave signal transmission

2022 ◽  
Vol 43 (1) ◽  
pp. 012302
Author(s):  
K. S. Zhuravlev ◽  
A. L. Chizh ◽  
K. B. Mikitchuk ◽  
A. M. Gilinsky ◽  
I. B. Chistokhin ◽  
...  
Keyword(s):  
Schottky Barrier ◽  
Transmission Lines ◽  
High Power ◽  
High Speed ◽  
Signal Transmission ◽  
Mesa Structure ◽  
Microwave Characterization ◽  
Absorbing Layer ◽  
Analog Transmission

Abstract The design, manufacturing and DC and microwave characterization of high-power Schottky barrier InAlAs/InGaAs back-illuminated mesa structure photodiodes are presented. The photodiodes with 10 and 15 μm mesa diameters operate at ≥40 and 28 GHz, respectively, have the output RF power as high as 58 mW at a frequency of 20 GHz, the DC responsivity of up to 1.08 A/W depending on the absorbing layer thickness, and a photodiode dark current as low as 0.04 nA. We show that these photodiodes provide an advantage in the amplitude-to-phase conversion factor which makes them suitable for use in high-speed analog transmission lines with stringent requirements for phase noise.

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