scholarly journals Effects of High Forage/Concentrate Diet on Volatile Fatty Acid Production and the Microorganisms Involved in VFA Production in Cow Rumen

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 223 ◽  
Author(s):  
Lijun Wang ◽  
Guangning Zhang ◽  
Yang Li ◽  
Yonggen Zhang
Keyword(s):  
Block Design ◽  
Fatty Acid Production ◽  
Key Enzymes ◽  
Ferredoxin Oxidoreductase ◽  
Randomized Complete Block Design ◽  
Pyruvate Ferredoxin Oxidoreductase ◽  
Butyrate Kinase ◽  
The Difference ◽  
High Forage ◽  
Sampling Times

The objectives of this study were to investigate the difference in the mechanism of VFAs production combined with macrogenome technology under different forage-to-concentrate ratios and sampling times. Six ruminally cannulated Holstein cows were used in a randomized complete block design. The high forage (HF) and high concentrate (HC) diets contained 70 and 35% dietary forage, respectively. The results showed that pH was affected by sampling time, at 4 h after feeding had lower value. Excepted for acetate, the VFAs was increased with forage decreased. Propionate formation via the succinic pathway, in which succinate CoA synthetase (EC 6.2.1.5) and propionyl CoA carboxylase (EC 2.8.3.1) were key enzymes, and significantly higher in HC treatment than in HF treatment, Selenomonas, Ruminobacter, Prevotella, and Clostridium were the main microorganism that encodes these key enzymes. Butyrate formation via the succinic pathway, in which phosphate butyryltransferase (EC 2.3.1.19), butyrate kinase (EC 2.7.2.7) and pyruvate ferredoxin oxidoreductase (EC 1.2.7.1) are the important enzymes, Prevotella and Bacteroides played important role in encodes these key enzymes. This research gave a further explanation on the metabolic pathways of VFAs, and microorganisms involved in VFAs production under different F:C ration, which could further reveal integrative information of rumen function.

scholarly journals Effect of energy source and level, animal age, and sex on the flavor profile of sheep meat12

2020 ◽  
Vol 4 (2) ◽  
pp. 1140-1147
Author(s):  
Jerad R Jaborek ◽  
Henry N Zerby ◽  
Macdonald P Wick ◽  
Francis L Fluharty ◽  
Steven J Moeller
Keyword(s):  
Energy Source ◽  
Block Design ◽  
Flavor Intensity ◽  
Ewe Lambs ◽  
Age Related ◽  
Randomized Complete Block Design ◽  
Ad Libitum ◽  
Eating Experience ◽  
Dietary Treatments ◽  
High Forage

Abstract The effects of dietary energy source, energy level, sheep age, and sheep sex on flavor and off-flavor intensity were evaluated. Consumer panelists, with previous lamb-eating experience, assessed lamb flavor and off-flavor intensity on a 100-point, end-anchored scale (0 = very mild to 100 = very intense), with off-flavor being defined as anything different than lamb flavor. Lamb longissimus thoracis (LT) and whole, boneless ground shoulder (GS) formed into patties were evaluated. Trial 1 was a randomized complete block design with a 3 × 2 factorial arrangement of treatments. Sheep age (ewe lambs, yearling ewes, and mature ewes; n = 16/age) and ad libitum access to diets [alfalfa pellets (AP) or whole-shelled corn (WSC100)] were treatments. The LT from mature ewes had a greater (P ≤ 0.02) off-flavor intensity when compared with yearling ewes and ewe lambs. Ground shoulder from sheep raised on AP had a greater lamb flavor (P ≤ 0.04) and off-flavor (P ≤ 0.04) intensity than GS from sheep consuming WSC100. Trial 2 was a randomized complete block design with a 3 × 2 × 2 factorial arrangement of treatments. Three dietary treatments [AP, WSC100, and restricted intake of whole-shelled corn to 85% of ad libitum (WSC85)], lamb sex (ewes and wethers; n = 48/sex), and lamb age [short fed, 177 ± 16.6 d of age and 93 ± 20.5 d on feed (DOF); long fed, 294 ± 7.0 d of age and 219 ± 3.8 DOF]. Flavor intensity of the LT was greater (P ≤ 0.05) from lambs offered AP when compared with lambs offered WSC85, whereas lamb flavor of the LT from lambs fed WSC100 was intermediate and not different from the lamb flavor of the LT of lambs fed AP or WSC85. The LT of long-fed lambs had a greater (P ≤ 0.01) lamb flavor and off-flavor intensity when compared with short-fed lambs. Lambs offered AP resulted in a GS with greater lamb flavor intensity (P ≤ 0.01) when compared with lambs offered WSC85 and WSC100, with no diet influence on GS off-flavor intensity. Long-fed lambs produced GS with a greater lamb flavor (P ≤ 0.01) and off-flavor (P ≤ 0.01) intensity when compared with GS from short-fed lambs. Results from the two trials indicate lamb flavor and off-flavor intensity were greater from sheep offered a high-forage (AP) diet when compared with a high-concentrate (WSC) diet. Lamb flavor intensity increased as age of the sheep at harvest increased, suggesting dietary management and associated age-related effects at harvest will influence consumer perception of lamb flavor.

115 Stability of Thiamine Supplied by Vitamin Premix and Yeast Ingredients in Canned Cat Food

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 61-62
Author(s):  
Amanda N Dainton ◽  
Markus F Miller ◽  
Brittany White ◽  
Leah K Lambrakis ◽  
Charles G Aldrich
Keyword(s):  
Binding Protein ◽  
Block Design ◽  
Random Effect ◽  
Comparison Test ◽  
Randomized Complete Block Design ◽  
Thiamine Content ◽  
The Difference ◽  
Retort Processing ◽  
Post Hoc

Abstract Despite numerous process modifications, thiamine retention of canned cat food has not improved. Yeasts possess a binding protein which may provide a thiamine form able to withstand retort processing. Therefore, the objective was to evaluate dried yeasts as thiamine sources in canned cat food. Treatments were arranged as a 2ｘ4 factorial with 2 levels of vitamin premix (with or without) and 4 sources of yeast (none (NY), LBV, BY, or EA). Inclusion of LBV matched the thiamine contribution from the vitamin premix and BY and EA were capped at 5%. Three replicates were processed in a horizontal still retort to an average total F0 of 79.23 minutes. Thiamine degradation was calculated as the difference between pre- and post-retort thiamine contents. Data were analyzed as a randomized complete block design with pre-retort thiamine content as a covariate and production day as a random effect. The Fisher’s LSD post hoc comparison test was performed with ⍺ = 0.05. On average, experimental formulas retained 33.75% thiamine. Thiamine degradation was not affected by the inclusion of vitamin premix (P = 0.0670), but was affected by the inclusion of yeast (P = 0.0232) and the interaction between vitamin premix and yeast (P = 0.0002). Thiamine loss between NY (-31.3 mg/kg DM) and BY (-33.8 mg/kg DM) were similar (P > 0.05) whereas EA (-40.5 mg/kg DM) and LBV (-55.6 mg/kg DM) lost more (P < 0.05) thiamine than NY. The treatment of EA with vitamin premix (-70.3 mg/kg DM) lost more (P < 0.05) thiamine than no yeast, BY, or EA without vitamin premix (average -17.4 mg/kg DM) and all others (average -57.3 mg/kg DM) were intermediate (P > 0.05). Inclusion of vitamin premix with yeast did not minimize thiamine loss and BY exhibited similar thiamine degradation as intrinsic thiamine in standard ingredients.

scholarly journals Study on Nutrient Balances of Newly Developed Lowland Rice Fields at Kleseleon Village, Malaka District, Nusa Tenggara Timur

2020 ◽  
Vol 25 (3) ◽  
pp. 137
Author(s):  
Sukristiyonubowo - ◽  
Damasus Riyanto
Keyword(s):  
Rice Straw ◽  
Block Design ◽  
Lowland Rice ◽  
Nutrient Inputs ◽  
Nutrient Balances ◽  
Application Rates ◽  
Randomized Complete Block Design ◽  
Potential Acid ◽  
The Difference ◽  
Sulfate Soils

The land allocated for newly opened lowland rice can be from ultisols, oxisols, inceptisols, and potential acid sulfate soils. The study was aimed to evaluate nitrogen (N), phosphorous (P), and potassium (K) inputs – outputs of newly developed lowland rice under different treatments and to validate the N, P, and K recommended applications rate according to N, P, and K balances. The study was conducted in Kleseleon village, Malaka District. Five treatments were tested namely T0: farmers practices, T1: NPK at recommendation rate + Rice straw compost, T2: NPK at recommendation rate + Smart + Rice straw compost, T3: ¾ NPK at recommendation rate + Smart + Rice straw compost, and T4: NPK at recommendation rate + Smart + Rice straw compost. The treatments were arranged in a randomized complete block design with three replications. The nutrient balances were constructed according to the difference between nutrient inputs and losses. The results indicated that there were surplus N, P, and K across the treatments and more than adequate to replace N, P, and K removed by harvest products. The suggested N, P, and K application rates were 100 kg urea, 100 kg SP-36, and 100 kg KCl with the addition of compost 3 Mg ha-1 season-1.

scholarly journals Growth and accumulation of nutrients in organic jambu

2018 ◽  
Vol 9 (2) ◽  
pp. 287-291
Author(s):  
Leilson Costa Grangeiro ◽  
Gilberta Carneiro Souto ◽  
Valdivia Fátima Lima Sousa ◽  
Jader Vieira Carneiro ◽  
Jorge Luiz Alaves Silva ◽  
...  
Keyword(s):  
Experimental Design ◽  
Block Design ◽  
Nutrient Accumulation ◽  
Purple Flower ◽  
Yellow Flower ◽  
Nutrient Demand ◽  
Federal Institute ◽  
Randomized Complete Block Design ◽  
Calcium Magnesium ◽  
Sampling Times

The objective of this study was to evaluate the growth and accumulation of nutrients in accessions of organic jambu. The experiment was conducted in the didactic garden of the Federal Institute of Pará, Campus Castanhal, in the period May and June 2015. The experimental design was a randomized complete block design with a 2 x 5 factorial arrangement with four replicates. The treatments were a combination of two jambu accessions (Purple Flower and Yellow Flower) and five sampling times (10, 18, 26, 34 and 42 days after transplanting - DAT). The period of greatest growth and nutrient demand was for access to Purple Flower from 18 to 34 DAT and for the Yellow Flower from 26 to 42 DAT. Nutrient accumulation by the two accesses of jambu, in decreasing order, was: potassium, nitrogen, calcium, magnesium and phosphorus.

Effects of Trichoderma harzianum Strain T-22 Sprays on Incidence of Sooty Blotch and Fly Speck of Apple and Botrytis Fruit Rot of Raspberry

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 491f-492
Author(s):  
Richard K. Kiyomoto
Keyword(s):  
Trichoderma Harzianum ◽  
Block Design ◽  
Fruit Rot ◽  
Red Raspberry ◽  
Evaluation Period ◽  
Sooty Blotch ◽  
Randomized Complete Block Design ◽  
Sooty Blotch And Flyspeck ◽  
The Difference ◽  
Four Blocks

Tests were made to determine the effectiveness of Trichoderma harzianum T-22 sprays in controlling sooty blotch (Zygophiala jamaicensis) and fly speck (Gloeodes pomigena) in five experimental scab-resistant apple varieties and botrytis fruit rot in five fall-bearing red raspberry cultivars. T-22 and S-12 surfactant used in these studies were supplied by BioWorks, Inc., Geneva, N.Y. Treatments applied to runoff on foliage and fruit were: unsprayed; T22 (1.2 g·L–1), T22 + S-12 (1.2 g·L–1 + 0.5 g·L–1), Captan 50W +T22 (1.2 g·L–1 + 1.2 g·L–1), and Captan 50W (1.1 g·L–1). Raspberry treatments substituted Rovral for Captaon 50W. Cultivars in the apple and raspberry trials were planted in a randomized complete block design with three blocks in the apple and four blocks in the raspberry planting. All spray treatments were applied to each cultivar in a block. Apples were treated on 5 Aug., 19 Aug, 2 Sept., and 16 Sept. and fruit were evaluated as cultivars ripened from 29 Sept. to 17 Oct. Raspberries were treated on 30 July (34% to 86% bloom), 14 Aug., 28, Aug., and 9 Sept. Evaluation for Botrytis was made throughout this period and data are presented for 12 Sept. Incidence of sooty blotch and flyspeck were ≈50% lower in T22 treatments than in unsprayed controls, but the difference was not significant. Captan treatments had significantly lower incidence of sooty blotch. Captan and T22 treatments had less flyspeck than the unsprayed controls, but the differences were not significant. Significant cultivar × treatment interactions may have resulted from the prolonged evaluation period and obscured differences in treatment effects In the fall raspberries 10% of untreated, 5% of T22 and T22 + S-12-treated, and 2% of Rovral and Rovral + T22 fruit were infected with Botrytis. These differences were highly significant.

DISTRIBUSI DAN IDENTIFIKASI POPULASI SINTRONG (Crossocephalum crepidiodes.Benth) RESISTEN PARAKUAT PADA LAHAN JAGUNG (Zea mays) DI KABUPATEN DAIRI

2017 ◽  
Vol 4 (2) ◽  
pp. 149-161
Author(s):  
Berton Sianturi
Keyword(s):  
Zea Mays ◽  
Dose Response ◽  
High Resistance ◽  
Block Design ◽  
Recommended Dose ◽  
Second Step ◽  
Resistance Level ◽  
Susceptible Population ◽  
Randomized Complete Block Design ◽  
Susceptible Populations

Crassocephalum crepidioides on Cornfields in Dairi Regency had been reported tobecome more difficult to control using paraquat. The objective of the research was todetermine the characteristics and the distribution of C.crepidioides resistant to paraquatin cornfields. The experiment was carried out in two steps, the first step was screeningthe population of C. crepidioides with paraquat at the recommended dose, and the secondstep, dose-response experiment for the resistance level of C. crepidioides population withdose 0, 76, 152, 304,5, 609, 1218, and 2436 g.ai /ha. In the first step experiment, paraquatdichloride was applied at 280 g.ai/ha. The treatments were arranged in a randomized blockdesign with 3 replication. The second step experiment was that the resistant populationsconfirmed in the first experiment were sprayed for their dose-response. The treatmentswere arranged in a randomized complete block design (CRBD). The results showed thatof 30 populations of C. crepidiodes, 19 populations (63.3%) were categorized to beresistant with the mortality ranging from 10.84% to 52.08%, and 11 populations (36.7%),was categorized as high resistance with mortality of 0% to 9.21%. The level ofresistance (R/S) of R-C25, R-C27, and R-C30 populations of C. crepidioides were 12,3,14,86, and 24,83 times consecutively, compared with the susceptible population. Thenumber of C. crepidioides chlorophyl leaves in susceptible populations was significantlylower than that of a resistant populations.

Response of corn (Zea mays L.) to various organic-based fertilizers in marginal upland of Sta. Rita, Samar

2017 ◽  
pp. 31-43
Author(s):  
Berta Ratilla ◽  
Loreme Cagande ◽  
Othello Capuno
Keyword(s):  
Block Design ◽  
Management Strategies ◽  
Chemical Properties ◽  
Organic Fertilizers ◽  
Growth And Yield ◽  
Physical And Chemical Properties ◽  
Total N ◽  
Randomized Complete Block Design ◽  
Physical And Chemical ◽  
And Chemical Properties

Organic farming is one of the management strategies that improve productivity of marginal uplands. The study aimed to: (1) evaluate effects of various organic-based fertilizers on the growth and yield of corn; (2) determine the appropriate combination for optimum yield; and (3) assess changes on the soil physical and chemical properties. Experiment was laid out in Randomized Complete Block Design, with 3 replications and 7 treatments, namely; T0=(0-0-0); T1=1t ha-1 Evans + 45-30-30kg N, P2O5, K2O ha-1; T2=t ha-1 Wellgrow + 45-30-30kg N, P2O5, K2O ha-1; T3=15t ha-1 chicken dung; T4=10t ha-1 chicken dung + 45-30-30kg N, P2O5, K2O ha-1; T5=15t ha-1 Vermicast; and T6=10t ha-1 Vermicast + 45-30-30kg N, P2O5, K2O ha-1. Application of organic-based fertilizers with or without inorganic fertilizers promoted growth of corn than the control. But due to high infestation of corn silk beetle(Monolepta bifasciata Horns), its grain yield was greatly affected. In the second cropping, except for Evans, any of these fertilizers applied alone or combined with 45-30-30kg N, P2O5, K2O ha-1 appeared appropriate in increasing corn earyield. Soil physical and chemical properties changed with addition of organic fertilizers. While bulk density decreased irrespective of treatments, pH, total N, available P and exchangeable K generally increased more with chicken dung application.

Effect of Bio and Organic Fertilizers on Pumpkin's (Cucurbita pepo L.) Leave Content of N, P, and K

2020 ◽  
Vol 10 (1) ◽  
pp. 241-245
Author(s):  
Ammar Hameed Madi ◽  
Jawad A. Kamal Al-Shibani
Keyword(s):  
Cucurbita Pepo ◽  
Sandy Loam ◽  
Block Design ◽  
Organic Fertilizers ◽  
Flowering Stage ◽  
College Of Agriculture ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Loam Soil ◽  
Four Levels

This study was conducted to investigate the effect of bacterial bio-fertilization A. chroococcum and P. putide and four levels of compost (0, 1, 2, 3) tons.h-1 on the leaves content of N.P.K elements. The experiment was carried out in one of the greenhouses of the College of Agriculture - University of Al-Qadisiyah during fall season 2018-2019. It designed in accordance with the Randomized Complete Block Design (RCBD) with three replicates in sandy loam soil. The means of treatments were compared with the least significant difference (LSD) at (5)% probability level. The results present that the treatments of A. chroococcum, P. putide and compost at (3) tons.kg-1 significantly increases the leaves content of K.P.K compared to all other treatments in the flowering stage (4.970, 0.5000, and 4.930) mg.kg-1, respectively. This treatment was followed by the effect of the treatment of A. chroococcum and compost at (3) tons.kg-1, which increases the values of all traits except the leaf content of (P). Bio-fertilizer with P. putide + A. chroococcum significantly increases the leaves' content of P.

Growth and Branching Responses of Portulaca grandiflora to Foliar Applications of Chemical Growth Regulators

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 447c-447
Author(s):  
T.J. Banko ◽  
M.A. Stefani
Keyword(s):  
Growth Regulators ◽  
Growth Habit ◽  
Stem Elongation ◽  
Block Design ◽  
Shoot Elongation ◽  
Main Shoot ◽  
Randomized Complete Block Design ◽  
Portulaca Grandiflora ◽  
Prostrate Growth ◽  
Secondary Shoot

During container production of portulaca, growth of long, prostrate, sparsely branched stems makes handling of plants difficult, and reduces their commercial appeal. Growers prefer to minimize shoot elongation while increasing branching to provide a full, compact plant. The objectives of this study were to evaluate growth regulators for promotion of branching and inhibition of stem elongation. Container-grown plants ≈21 cm in diameter were treated with sprays of ProShear (benzylaminopurine) at 62.4, 125, and 250 ppm; Promalin (benzyaminopurine + gibberellins 4+7) at 125, 250, and 500 ppm; Atrimmec (dikegulac) at 250, 500, and 750 ppm; and Florel (ethephon) at 250, 500, and 750 ppm. These treatments were compared with untreated controls in a randomized complete-block design. Main shoot lengths were measured at 16, 31, and 51 days after treatment (DAT). Numbers of new shoot breaks were counted 16 DAT. The growth habit, that is, tendency to grow upright or prostrate, was also evaluated 16 DAT. The most-effective material for retarding primary shoot elongation and for stimulating secondary shoot development was ProShear. At 16 DAT, 250 ppm ProShear reduced shoot elongation by 25% compared to control plants. This treatment also increased the number of secondary shoot breaks by 143%. Promalin increased the number of new shoot breaks, but it also increased the lengths of all shoots. High rates of Florel and Promalin caused shoots to grow predominantly upright rather then prostrate. ProShear, however, caused more prostrate growth as rate increased.

Influence of Nitrogen and Potassium on Three Zoysiagrasses

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 516b-516
Author(s):  
James N. McCrimmon
Keyword(s):  
Gulf Coast ◽  
Block Design ◽  
Nutrient Content ◽  
Zoysia Japonica ◽  
Tissue Samples ◽  
Color Quality ◽  
Randomized Complete Block Design ◽  
Color Density ◽  
Minimal Research ◽  
Micronutrient Fertilizer

Zoysiagrass has great potential for use in the Gulf Coast states as a turfgrass. There has been minimal research on the nitrogen (N) and potassium (K) fertility response of zoysiagrass and the effect on turf color, quality, and nutrient content. The objective of this study was to evaluate the effects of N and K fertility on zoysiagrass. A study was conducted on three zoysiagrasses: Zoysia japonica × Z. tenuifolia Willd. ex Trin. (`Emerald'); Z. japonica Steud. (`Meyer'); and Z. matrella. The N and K treatment combinations consisted of high (H) and low (L) rates of N and K at the following levels: N levels of 454 and 227 g N/92.9 m2 per month and K levels of 454 and 227 g N/92.9 m2 per month. The treatment combinations were (N and K): HH, HL, LH, and LL and were applied in two split applications monthly from July through November. The study was a randomized complete-block design with three replications. All plots received two applications of a micronutrient fertilizer (late June and August), were irrigated as needed, and maintained at a height of 3.8 cm. Color, density, texture, uniformity, and quality were determined visually for each month. Plant tissue samples were collected (September) and analyzed for macronutrient and micronutrient contents. There were significant differences for color, density, and quality in the following months: September (color and density); October (quality); and November (color and quality). There were differences in leaf texture for all months. There were significant differences for N, magnesium (Mg), and K contents but there were no differences for any micronutrient. This study indicated that all three zoysiagrasses provided acceptable color and quality during the summer and fall, and that N and K rates affected N, K, and Mg contents in the plant.

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