scholarly journals Effects of Dietary Vibroactivated Clinoptilolite Supplementation on the Intramammary Microbiological Findings in Dairy Cows

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 202 ◽  
Author(s):  
Dražen Đuričić ◽  
Tomislav Sukalić ◽  
Franjo Marković ◽  
Predrag Kočila ◽  
Ivona Žura Žaja ◽  
...  

The aim of this study was to determine the effects of dietary vibroactivated clinoptilolite supplementation on the intramammary microbiological findings in dairy cows, causative agents of potential intramammary infection, and their sensitivity to antibiotics. Cows (n = 78) were randomly divided into two groups: CPL-treated group that received clinoptilolite (CPL) in-feed (n = 38), i.e., 50 g natural powdered zeolite CPL, twice daily from the seventh month of pregnancy to 75 days after calving, and the control group (CON) of untreated cows (n = 40). Milk samples were taken from each cow on days 7, 25, 45, and 75 postpartum. The following causative pathogens were isolated in 86 udder quarters: Staphylococcus aureus in 5.81% of positive samples, Staphylococcus spp. 9.32%, coagulase-negative Staphylococcus (CNS) 22.09%, Streptococcus uberis 13.95%, Streptococcus agalactiae 1.16%, Streptococcus sp. 3.49%, Escherichia coli 8.13%, Enterococcus spp. 6.98%, Corynebacterium spp. 11.63%, Pasteurella sp. 10.47%, Serratia spp. 2.33%, and Arcanobacterium pyogenes, Citrobacter sp., Prototheca sp., and yeasts each in 1.16% of samples. Additionally, 3.87% of environmental microflora samples (n = 47) and 89.06% of udder samples (n = 1083) were bacteriologically negative. The most effective antibiotics were cefoperazone and amoxicillin-clavulanic acid, while cloxacillin and tetracycline were the least effective antibiotics in both groups. In the clinoptilolite supplemented (CPL) group (n = 38) of 14 cows, nine causative agents of mastitis were isolated in 27 quarters, while in the control (CON) group (n = 40) of 24 cows, 13 causative agents of mastitis were isolated in 59 quarters. Cows from the CON group had a 1.96 times higher risk of intramammary infection than cows from the CPL group during the observation period (odds ratio = 1.96, p = 0.0031; 95% CI = 1.2570–3.0770).

2014 ◽  
Vol 97 (12) ◽  
pp. 7426-7436 ◽  
Author(s):  
I.M.T. Thompson ◽  
S. Tao ◽  
A.P.A. Monteiro ◽  
K.C. Jeong ◽  
G.E. Dahl

Author(s):  
Bridget Drew ◽  
J A Foulkes ◽  
Jill F B Altman

The results of studies undertaken outside the United Kingdom suggest that the inclusion of 50 g of 30% protected DL Methionine (Ketionin) into the ration of conventionally fed dairy cows improves feed utilisation, milk yield, fertility and health. For instance (Spørndly (1981) in Sweden measured the weight of cows during the experimental period and found that the weight loss in the control group was twice that of the cows that received Ketionin. In West Germany a significant increase in milk of 2.0 kg per day was obtained by Kaufmann and Hagemeister (1980) and similar results were reported by Kuther (1982). Stoikov (1978) in studies on 673 cows in Bulgaria showed an improvement in calving rate from 37% in the control group to 55% in the treated group and Daugaard (1978) working in Denmark reported that the inclusion of Ketionin reduced the incidence of Ketosis.


1990 ◽  
Vol 78 (03) ◽  
pp. 132-134 ◽  
Author(s):  
A.V. Williamson ◽  
W.J. Crawford ◽  
B. Rennie ◽  
W.L. Mackie

AbstractOverall reproductive performance and associated periparturient disorders were monitored in a herd of British Friesian cross dairy cows from September 1987 to August 1988. Pregnancy diagnosis and cyclical status of the herd was determined by palpation of the ovaries per rectum. A total of 49 (110) cows were randomly treated with Sepia 200c either on day 14(19) or 21 days (30) postpartum.Statistical analysis of the results was based on the differences between the control and combined Sepia-treated groups. Although there were significant differences in the proportion of heifers calving in each group, there was no significant difference in periparturient disorders or during the pre-service period.However, in the Sepia Group that held a lower proportion of heifers, a significant difference was found in the conception rate to 1st service (47%), the percentage of cows in calf (90%) and total culling rate (10%) compared to the Control Group (26%, 72% and 28% respectively). A reduction (non significant) was found between the Sepia and Control services to conception (0.2), calving to conception interval (5 days) and calving index (2 days).The results of this pilot study appear to demonstrate a difference in effect between the Sepia-treated Group and Control Group. The study has been extended to treat a larger number of dairy cows to examine the reproducibility of these results.


Author(s):  
Rana S. Al-Taweel

Septicemia is a serious bloodstream infection; it can quickly become life-threatening.  The current study aimed to isolate and identify the causative agents of septicemia cases with reference to the antibiogram test.  52 blood samples were collected from a number of incomings and those who lie in Hilla general hospital. After incubation and culturing on suitable media, 30 samples gave growth of one or two species of bacteria. A total of 33 bacterial isolates were obtained, most of them (16 isolates) were belong to coagulase-negative Staphylococcus spp., followed by E. coli (6 isolates), Pseudomonas sp. (5 isolates), Streptococcus sp. (2 isolates), and one isolate for each of Staphylococcus aureus,  Streptococcus pneumonia, Acinetobacter sp., and Listeria monocytogenes. When antibiotics sensitivity test was accomplished, most Staphylococcus spp. were sensitive for daptomycin and had high resistance to both of vancomycin and clindamycin, whereas Staphylococcus aureus was sensitive for most the used antibiotics. Half of E. coli isolates were sensitive, while the second half were resistant to the used antibiotics. Imipenem inhibited the growth of all Pseudomonas isolates, whereas 80% of them were resistant to amikacin. The epidemiology of bacteremia is altering with the aging of the population, shifts in healthcare, and progress in medicine, such as increased use of immunosuppressive treatment, intravascular devices, and invasive procedures.


2020 ◽  
Vol 9 (11) ◽  
pp. e1749119607
Author(s):  
Renata Cristina Defiltro ◽  
Patricia Glombowsky ◽  
Lilian Kolling Girardini ◽  
Julcimar Machado Maciel ◽  
Marcelo Vedovatto ◽  
...  

The aim of this study was to determine whether the addition of a homeopathic product to dairy cow feed would improve health and production efficiency, as well as composition and quality of milk. The product tested here was produced to stimulate immunity and indirectly to prevent the colonization of the mammary gland by bacteria, thereby decreasing somatic cell counts and bacteria in cow’s milk. Fifty dairy cows were used, divided into two groups: Control (n = 25) and Treated (n = 25). For 90 consecutive days, 50 grams of homeopathic product (treated group) was added to the concentrate; 50 g of the product vehicle (limestone) was added to the concentrate of the control cows. We performed composition and quality analysis on the milk (days 1, 15, 30, 45, 60, 75, and 90) as well as blood collection to carry out hematological and metabolism analyses (days 1, 30, 60, and 90). We found that fat content in the milk cows consuming the homeopathic agent was greater than that of the control group. Because of this increased fat content, there was a tendency towards higher total solids content in treated cows. A treatment effect was found in terms of total bacterial count (TBC); that is, there were lower TBCs in milk of treated animals than in control animals. There was a trend of a treatment effect versus day for somatic cell count (SCC); that is, there were lower SCC in animals that consumed the homeopathic on days 15 and 30 of the experiment. There was no difference between groups in terms of milk production; however, there was a negative correlation between milk production and TBC or SCC in the milk of cows that consumed homeopathic product. Animals in the treated group had lower total leukocyte and lymphocyte counts than did the control group, as well as a tendency toward lower neutrophil counts in these animals. Several bacteria were isolated from the cows' milk during the experimental period, with no treatment effect. In particular, we isolated Corynebacterium spp., Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus hyicus in greater numbers of cows compared to other etiologic agents. Based on these results, we conclude that consumption of the homeopathic product by dairy cows had positive effects on milk quality.


2020 ◽  
Vol 11 (2) ◽  
pp. 408-420
Author(s):  
Carlos Leyva Orasma ◽  
Jesús Jaime Benitez-Rivas ◽  
Juan Luis Morales Cruz ◽  
Cesar Alberto Meza-Herrera ◽  
Oscar Ángel-García ◽  
...  

The aim was to evaluate if 1-2 propanodiol plus calcium propionate (glycogenic precursor) supplementation during the transition period in high yielding dairy cows reduces metabolic and reproductive dysfunctions during early lactation. Cows (n=202) where divided into two homogeneous groups regarding number of lactations and body condition score. 1) Treated Group (GG; n=112) received 60g/cow/day  15d of a glycogenic precursor during the transition period. 2) Control Group (GC; n = 90) received no treatment. Postpartum levels of BHB (GG= 0.9±0.2 mmol/L vs GC =1.3±0.2 mmol/L; P < 0.05), and NEFA (GG= 0.6 ± 0.1 mEq/L vs GC = 0.8 ± 0.1 mEq/L; P < 0.05) were higher for GC. Likewise, GC-cows had a higher percentage of retained placenta (23% vs. 13%; P ≤ 0.06) subclinical ketosis (GG = 10%, GC = 56%; P < 0.05), and mastitis (GG = 8%, GC = 16%; P < 0.05). Metritis, dystocia, abortions, clinical ketosis, hypocalcemia and ruminal acidosis showed no differences. Administration of a glycogenic precursor during the transition period demonstrated a positive effect upon BHB and NEFA blood levels during early lactation. Also, levels of subclinical ketosis and retained placenta were reduced.


2015 ◽  
Vol 31 (3) ◽  
pp. 349-364 ◽  
Author(s):  
H.A. Bakr ◽  
M.S. Hassan ◽  
N.D. Giadinis ◽  
N. Panousis ◽  
D. Ostojic-Andric ◽  
...  

Data concerning the effect of probiotics supplementation on many parameters concurrently at the same cows are lacking. Therefore, the objective of this experiment was to investigate the effects of Saccharomyces cerevisiae feeding on rumen, blood and milk parameters together in highproducing dairy cattle during the transition and early lactation period. Sixteen clinically healthy Holstein cows were divided into 2 groups: a control group of 6 cows and a probiotics-fed group of 10 cows. Rumen fluid and blood samples were collected 21 days before the expected calving as well as 7, 15, 30, 45 and 60 daysin- milk (DIM). Milk yield for each animal was recorded every 2 weeks. Individual milk samples were collected 15, 30, 45 and 60 DIM. Ruminal pH and rumen ammonia nitrogen were significantly lower, whereas total volatile fatty acids were significantly higher in yeast-fed animals compared with controls throughout the study. Serum concentrations of total proteins and globulins were higher, while albumins were lower in the yeast-treated group. Serum glucose levels were significantly higher in yeast-supplemented animals. Serum triglycerides, high density lipoproteins, and low density lipoproteins concentrations were lower, with cholesterol being significantly lower in the treated group. Milk production and milk fat percentage were higher, whereas milk protein percentage and somatic cell count were decreased in yeast-supplemented cows throughout the study. These results suggest that supplementation of S. cerevisiae to dairy cows rations during transition and early lactation period improve their health and milk production parameters.


1999 ◽  
Vol 62 (11) ◽  
pp. 1354-1357 ◽  
Author(s):  
S. P. OLIVER ◽  
M. J. LEWIS ◽  
B. E. GILLESPIE ◽  
S. J. IVEY ◽  
L. H. COLEMAN ◽  
...  

A trial was conducted for 12 months in a herd of 120 Holstein cows in order to determine the efficacy of a teat disinfectant, which contained a phenolic combination, for the prevention of bovine intramammary infections during lactation. Postmilking teat disinfection was compared to a negative control using a split-udder experimental design. The percentage of quarters newly infected by mastitis pathogens was 45% lower in mammary glands with teats that had been dipped in the experimental teat disinfectant after milking than it was in undipped controls. New infections caused by Streptococcus uberis, Staphylococcus aureus, coagulase-negative Staphylococcus species, and Corynebacterium bovis were significantly lower in mammary glands with teats that had been dipped in the experimental teat disinfectant than in undipped controls. No statistical differences in the incidence of clinical mastitis between treatment groups were observed. No irritation or chapping of teats dipped in the experimental teat disinfectant were observed. The results of this study suggest that the experimental teat disinfectant containing a phenolic combination is an effective postmilking teat disinfectant for use in the prevention of new intramammary infections by both contagious and environmental mastitis pathogens.


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