scholarly journals Effects of Dietary Fiber Sources during Gestation on Stress Status, Abnormal Behaviors and Reproductive Performance of Sows

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 141 ◽  
Author(s):  
Shuangbo Huang ◽  
Jianfu Wei ◽  
Haoyuan Yu ◽  
Xiangyu Hao ◽  
Jianjun Zuo ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Peptide Yy ◽  
Control Diet ◽  
Group Versus ◽  
Gas Production ◽  
Stillbirth Rate ◽  
Antioxidant Power ◽  
Swelling Capacity ◽  
Abnormal Behaviors

Inclusion of fiber in gestation diets is a method for enhancing satiety and reducing abnormal behaviors in restricted feeding sows without providing excess energy. The purpose of this study was to use an in vitro-in vivo method to appraise the effects of two available unconventional dietary fiber resources during gestation on sows’ physio-chemical properties of diets, postprandial satiety, performance, abnormal behaviors, stress status and lactation feed intake under three different dietary treatments: control diet (CON diet), 5% resistant starch diet (RS diet), and 5% fermented soybean fiber diet (FSF diet) with a total of 78 (average parity 5) Landrace × Yorkshire sows. Results showed that swelling capacity was higher in the RS diet than in the CON or FSF diet. Meanwhile, the 48 h cumulative gas production and the final asymptotic gas volume after in vitro fermentation of gestation diets showed an increased trend (p = 0.07, p = 0.09, respectively) in the RS diet versus the CON or FSF diets. While the sows’ litter size, body weight, backfat or weaning-to-estrus interval were not affected (p > 0.05) by the three treatments during gestation, the RS group showed a decline in stillbirth number (p < 0.05) and stillbirth rate (p < 0.01) relative to the other two groups. Meanwhile, the proportion of standing was lower while the sow’s serum concentrations of PYY (peptide YY) and GLP-1 (glucagon-like peptide-1) were higher (p < 0.05) on day 70 of gestation in the RS group than in the CON or FSF group. Compared with the CON group, the RS group showed a downward tendency (p = 0.07) in the sows’ plasma cortisol concentration on day 70 of gestation. A comparison of oxidative and antioxidative indicators revealed an increase in the sows’ serum FRAP (ferric ion reducing antioxidant power) (p < 0.05) and a decrease of protein carbonyl (p < 0.05) on day 109 of gestation in the RS or FSF group versus the CON group. Overall, inclusion of 5% RS with greater swelling capacity in the gestation diet contributed to enhancing the postprandial satiety, alleviating the stress status, reducing the abnormal behaviors and thus lowering the stillbirth rate of sows.

PSXIV-14 Supplementation of high-grain diet with red osier dogwood affects in vitro digestibility and fermentation characteristics

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 477-477
Author(s):  
Wenzhu Yang
Keyword(s):  
Control Diet ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Rumen Acidosis ◽  
Barley Silage ◽  
And Control

Abstract Red osier dogwood (ROD) is a native shrub plant rich in phenolic compounds with antimicrobial properties. The objective of this study was to evaluate the effects of substituting barley silage with either raw ROD or ROD extract (RODE) in high-grain (HG) diet under a low media pH (5.8) on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures. The study was a completely randomized design with 4 treatments: 1) control diet (10% barley silage and 90% barley concentrate, DM basis), control diet supplemented with 2) monensin (30 mg/kg diet DM; positive control), 3) substitution of 3% ROD or 4) 3% RODE for an equal portion of silage. Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HG diet. Substrate ground (1 mm) was incubated for 24 h and the experiment repeated twice. The GP did not differ among treatments (147 ml/g DM), but the DMD differed (P &lt; 0.02) at highest for control (69.4%), lowest for ROD (58.4%) and intermediate for other treatments (64.1%). Total volatile fatty acid (VFA) concentration (mM) tended (P&lt; 0.08) to be lower with ROD (80.5) and monensin (80.1) than control (83.9). Acetate proportion was greater (P = 0.02) with ROD (46.2%) and RODE (46.9%) than control (42.4%) and monensin (42.3%). However, the propionate proportion was greater (P = 0.05) with monensin (32.1%) than other treatments (averaged 30.1%). Consequently, acetate to propionate ratio (A:P) of ROD (1.52) and RODE (1.56) was higher than monensin (1.32; P &lt; 0.01) and control (1.44; P &lt; 0.08). Differences in variables measured between ROD and RODE were minimal. These results indicated that the decreased DMD along with increased A:P with addition of ROD or RODE suggests that both ROD and RODE may be beneficial to HG fed cattle for reducing risk of rumen acidosis without negatively impacting fibre digestion.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

scholarly journals Effect of Commercial Slow-Release Urea Product on in Vitro Rumen Fermentation and Ruminal Microbial Community Using RUSITEC Technique

2021 ◽  
Author(s):  
Yongmei Guo ◽  
Ling Xiao ◽  
Long Jin ◽  
Sumei Yan ◽  
Dongyan Niu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dairy Cow ◽  
Protein Production ◽  
Control Diet ◽  
Gas Production ◽  
Microbial Protein ◽  
N Concentration ◽  
Slow Release Urea ◽  
Microbial N

Abstract Background The objectives of this study were to determine the effect of commercial slow-release urea (SRU) on in vitro fermentation characteristics, nutrient digestibility, gas production, microbial protein synthesis and bacterial community using rumen simulation technique (RUSITEC). The experiment was a completely randomized design with four treatments and four replications of each treatment. Treatments were: control diet (no SRU addition), control diet plus 0.28% SRU (U28), or plus 0.56% SRU (U56), and control diet that was modified for substituting with 0.35% SRU for equavelant soybean protein (MU35; dry matter [DM] basis). The experiment consisted of 8 days of adaptation and 7 days of data and sample collection. Rumen inoculum was obtained from three ruminally fistulated Angus cows fed the same diet to the substrate incubated. Results Digestibility of DM, organic matter (OM), crude protein (CP), fibre and starch was not affected, but daily production of gas (P < 0.07) and methane (P < 0.05) was quadratically changed with increasing SRU supplementation. The increase of SRU addition did not affect fermentation pH and total volatile fatty acid (VFA) production, whereas linearly (P < 0.01) decreased proportion of propionate, and linearly (P < 0.01) increased acetate to propionate ratio and ammonia nitrogen (N) concentration. The microbial N efficiency also linearly (P < 0.03) improved with increasing supplementation of SRU. In comparison with control diet, the dietary substitution of SRU for part of soybean meal increased (P < 0.05) the digestibility of DM, OM and CP and decreased (P < 0.02) the total gas production. The total VFA production and acetate to propionate ratio did not differ between control and MU35, whereas the proportion of butyrate was lower (P < 0.05) and that of branched-chain VFA was greater (P < 0.05) with MU35 than control diet. Total and liquid-associated microbial N production as well as ammonia N concentration were greater (P < 0.03) with MU35 than control diet. Observed OTUs, Shannon diversity index, and beta diversity of the microbial community did not differ among treatments. Taxonomic analysis revealed no effect of adding SRU on the relative abundance of bacteria at the phylum level, while at the genus level, the impact of SRU addition on microbial community was greater with MU35 either for liquid associate bacteria or feed particle-associated bacteria. Conclusions Supplementation of a dairy cow diet with SRU showed potential of increase in ammonia N concentration and microbial protein production, and change fermentation pattern to more acetate production. Adding SRU in dairy cow diet also showed beneficial effect on improving digestibility of OM and fibre. The results suggest that SRU can partially substitute soybean meal in dairy cow diet to increase microbial protein production without impairing rumen fermentation.

scholarly journals Ferric citrate decreases ruminal hydrogen sulphide concentrations in feedlot cattle fed diets high in sulphate

2013 ◽  
Vol 111 (2) ◽  
pp. 261-269 ◽  
Author(s):  
Mary E. Drewnoski ◽  
Perry Doane ◽  
Stephanie L. Hansen
Keyword(s):  
Hydrogen Sulphide ◽  
Rumen Fluid ◽  
Control Diet ◽  
Gas Production ◽  
Feedlot Cattle ◽  
Ferric Citrate ◽  
Ammonium Citrate ◽  
Ferric Ammonium Citrate ◽  
Ferric Ammonium

Dissimilatory reduction of sulphate by sulphate-reducing bacteria in the rumen produces sulphide, which can lead to a build-up of the toxic gas hydrogen sulphide (H2S) in the rumen when increased concentrations of sulphate are consumed by ruminants. We hypothesised that adding ferric Fe would competitively inhibit ruminal sulphate reduction. The effects of five concentrations and two sources (ferric citrate or ferric ammonium citrate) of ferric Fe were examinedin vitro(n6 per treatment). Rumen fluid was collected from a steer that was adapted to a high-concentrate, high-sulphate diet (0·51 % S). The addition of either source of ferric Fe decreased (P< 0·01) H2S concentrations without affecting gas production (P= 0·38), fluid pH (P= 0·80) orin vitroDM digestibility (P= 0·38) after a 24 h incubation. Anin vivoexperiment was conducted using eight ruminally fistulated steers (543 (sem12) kg) in a replicated Latin square with four periods and four treatments. The treatments included a high-concentrate, high-sulphate control diet (0·46 % S) or the control diet plus ferric ammonium citrate at concentrations of 200, 300 or 400 mg Fe/kg diet DM. The inclusion of ferric Fe did not affect DM intake (P= 0·21). There was a linear (P< 0·01) decrease in the concentration of ruminal H2S as the addition of ferric Fe concentrations increased. Ferric citrate appears to be an effective way to decrease ruminal H2S concentrations, which could allow producers to safely increase the inclusion of ethanol co-products.

Evaluation of a fat protection method (Ca-soap) with an in vitro gas production system

2006 ◽  
Vol 34 ◽  
pp. 125-129
Author(s):  
H Magaña-Sevilla ◽  
CA Sandoval-Castro ◽  
C. Capetillo-Leal
Keyword(s):  
Soybean Lecithin ◽  
Control Diet ◽  
Gas Production ◽  
Dietary Fats ◽  
Pennisetum Purpureum ◽  
Protection Method ◽  
Slaked Lime ◽  
Lime Solution ◽  
Sorghum Grain

SummaryAn in vitro gas production technique was used to evaluate a fat protection method (Ca-soap). The Ca-soaps were prepared by incorporation of oil into a slaked lime solution which had been prepared immediately before. The proportions employed were 10:1 (oil:slaked lime). A feed sample of 0.5g was incubated in a N-rich medium, using 6 ml ruminal liquor and 54 ml medium. A factorial design 2x3 (2 inoculum sources from cows fed with and without Ca-soap x 3 diets) was employed. Diets were A) Control: 70% grass (Pennisetum purpureum var. Taiwan), 30% sorghum grain; B) Soybean lecithin: 70% grass, 20% sorghum, 10% Lecithin; and, C) Ca-soap: 70% grass, 20% sorghum 10% Ca-soap (made from lecithin). Total gas production, DM and OM in vitro digestibility were measured. A 10% reduction (approximately) (P<0.05) on IVDMD and IVOMD with the oil diet was found, but no differences were found (P>0.05) between Control and Ca-soap diets. No effect was found due to inoculum source (P>0.05). Compared with the Control diet, the total gas production was reduced (P<0.05) in the oil diet and was intermediate in Ca-soap diet (P>0.05). It was concluded that Ca-soap diets are a source of rumen inert fat for both the in vitro incubation and the animals fed with this supplement. Ca-soap had no effect on IVDMD and IVOMD. In vitro gas production techniques can be useful tools to evaluate dietary fats at ruminal level.

scholarly journals Effect of mixture of herbal plants on ruminal fermentation, degradability and gas production

2020 ◽  
Vol 43 ◽  
pp. e48549
Author(s):  
Ahmed Mahmoud Abd El Tawab ◽  
Mostafa Sayed Abdellatif Khattab ◽  
Fatma Ibrahim Hadhoud ◽  
Mahmoud Mohamed Shaaban
Keyword(s):  
Organic Matter ◽  
Ph Value ◽  
Control Diet ◽  
Short Chain Fatty Acids ◽  
Culture Technique ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Ruminant Diets ◽  
Negative Effect

Reducing livestock negative environmental impacts get great interest in last years. So, present study was carried out to determine the effect of adding different levels of mixture of thyme and celery versus salinomycin on ruminal fermentation, gas production, dry, organic matter and fiber degradation. Four experimental treatments were used by in-vitro batch culture technique, as follow: 60% CFM, 40% clover hay (control), control diet + 2.5 gm thyme + 2.5 gm celery kg-1 DM (T1), control diet + 5 gm thyme + 5 gm celery kg-1 DM (T2), control diet + 10 gm thyme + 10 gm celery kg-1 DM (T3), control diet + 0.4 gm Salinomycin kg-1 DM (T4). Ruminal pH value was significantly increased (p < 0.05) with T4 compared with other treatments. While, the T4 recorded the lowest value (p < 0.05) for microbial protein, short chain fatty acids concentrations (SCFA), total gas production, dry matter and organic matter degradability (DMd and OMd) compared with other treatments. Fiber fraction degradability (NDFd and ADFd) appeared no significant variance (p > 0.05) between control and other treatments except for T1 that recorded the lowest value (p < 0.05). It is concluded that mixture of thyme plus celery could be alternate for ionophores in the ruminant diets to enhance ruminal fermentation, reducing gas production without any negative effect on nutrients degradability.

scholarly journals 163 Xylanase and fermentable xylo-oligosaccharides improve performance in grower-finisher pigs fed a corn-soybean meal based diet

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 91-92 ◽  
Author(s):  
Gustavo Cordero ◽  
Jae Cheol Kim ◽  
Natasha Whenham ◽  
Helen Masey-O’Neill ◽  
Saksit Srinongkote ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Feed Efficiency ◽  
Control Diet ◽  
Average Daily Gain ◽  
Gas Production ◽  
Large White ◽  
In Vitro Fermentation ◽  
Daily Gain ◽  
Average Body

Abstract Cecal inoculum collected from broilers fed a diet supplemented with xylanase showed increased gas production in an in vitro fermentation study with arabinoxylan-based substrates, indicating that intestinal microbiota can adapt to increase the capability to ferment arabinoxylans when pre-exposed to xylo-oligosaccharides (XOS) produced by xylanase. As the most abundant non-starch polysaccharides in a corn-soybean meal-based diet is arabinoxylan, this study tested the hypothesis that a blend of a xylanase and XOS will improve growth performance of pigs compared with those fed a diet supplemented with xylanase alone. One hundred twenty male crossbred (Landrace x Large white x Duroc) pigs (77 days average age; 30 kg average body weight -BW) were allocated to one of three treatments: CTR (control diet), XYL (CTR diet supplemented with 100 g/t of xylanase [Econase XT, AB Vista]) and XYL+XOS [CTR diet supplemented with 100 g/t of xylanase and XOS (Signis, AB Vista)]. Each treatment had ten replicates, with 4 animals each. The control diet was formulated with corn, soybean meal, DDGS and cassava meal as major ingredients. Pelleted diets were fed over 3 phases: grower (30–60 kg), finisher 1 (60–80 kg) and finisher 2 (80–100 kg). Average daily gain (ADG) and daily feed intake (ADFI) were measured from 30 to 100 kg and feed efficiency calculated (FCR). No treatment effects were observed on ADFI. Pigs offered the XYL+XOS had increased ADG (CTR = 1.058 vs XYL+XOS= 1.086 g/pig/d; P = 0.02) compared with those supplemented with xylanase alone (CTR = 1.058 vs XYL = 1.067 g/pig/d; P = 0.40). Feed efficiency was improved with XYL (2.65; P = 0.042) and XYL+XOS (2.60; P < 0.001) compared with CTR (2.71). These results suggest that xylanase in combination with XOS further improves daily gain and FCR compared with a xylanase alone. Interestingly the differences between XYL and XYL+XOS become more apparent in older animals, when the microbiome matures and possibly digests fiber more effectively.

scholarly journals Prebiotic Dietary Fiber and Gut Health: Comparing the In Vitro Fermentations of Beta-Glucan, Inulin and Xylooligosaccharide.

2017 ◽  
Author(s):  
Justin L. Caelson ◽  
Jennifer M. Erickson ◽  
Julie M. Hess ◽  
Trevor J. Gould ◽  
Joanne L. Slavin
Keyword(s):  
Dietary Fiber ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Gas Production ◽  
Gut Health ◽  
Beta Glucan ◽  
In Vitro Fermentation ◽  
Gastrointestinal Health ◽  
Oat Bran

Prebiotic dietary fiber supplements are commonly consumed to help meet fiber recommendations and improve gastrointestinal health by stimulating beneficial bacteria and the production of short-chain fatty acids (SCFAs), molecules beneficial to host health. The objective of this research project was to compare potential prebiotic effects and fermentability of five commonly consumed fibers using an in vitro fermentation system measuring changes in fecal microbiota, total gas production and formation of common SCFAs. Fecal donations were collected from three healthy volunteers. Materials analyzed included: pure beta-glucan, Oatwell (commercially available oat-bran containing 22% oat &beta;-glucan), xylooligosaccharides (XOS), WholeFiber (dried chicory root containing inulin, pectin, and hemi/celluloses), and pure inulin. Oatwell had the highest production of propionate at 12 h (4.76 &mu;mol/mL) compared to inulin, WholeFiber and XOS samples (p&lt;0.03). Oatwell&rsquo;s effect was similar to those of the pure beta-glucan samples, both samples promoted the highest mean propionate production at 24 h. XOS resulted in a significant increase in the genus Bifidobacterium after 24 h of fermentation (0 h: 0.67 OTUs; 24 h: 5.22 OTUs; p = 0.038). Inulin and WholeFiber increased the beneficial genus Collinsella, consistent with findings in clinical studies. All analyzed compounds were fermentable and promoted the formation of beneficial SCFAs.

scholarly journals Impact of Supplementation of Hemp Seeds (Cannavis sativa L.) on In Vitro Gas Fermentation Parameters and Digestibility using Foregut Fluid from Dromdary Camel

2020 ◽  
Vol 33 (1) ◽  
pp. 98-106
Author(s):  
Tahereh Mohammadabadi
Keyword(s):  
Biogas Production ◽  
Rumen Fluid ◽  
Control Diet ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Control Treatment ◽  
Cellulolytic Bacteria ◽  
Gas Fermentation

This experiment was conducted to investigate the effect of hemp seeds on in vitro biogas production, and digestibility of one-humped camel. Two female fistulated dromedary camels (300 kg) were fed with a forage diet (60 wheat straw and 40 alfalfa) for 1 month. Then rumen fluid was supplied from camels prior to the morning meal. Experimental samples were control diet and diet containing 10 % hemp seeds. Glass vials 100 ml containing 30 ml buffered rumen fluid was used. Gas production parameters were measured by exponential models and digestibility of foregut bacteria of camels were determined. The results showed that addition hemp seeds decreased potential of gas production (P<0.05), but gas production rate was not influenced (P>0.05). Actually digested organic matter and microbial biomass of diet containing hemp seeds were more than a control diet (P<0.05).While, hemp seeds supplementation had the greatest digestibility by cellulolytic bacteria of camel, after 24, 48 and 72 h incubation. The results also showed hemp seeds decreased ammonia nitrogen concentration as compared to the control treatment (P<0.05). Therefore, adding hemp seeds in the camel diet caused to reduce gas emission and ammonia nitrogen production in foregut and increased cellulolytic bacteria digestibility; so hemp seeds may be used in camel diets and will influence camel growth performance and decrease gas emissions in the environment.

Relation between in vivo and in vitro fermentation of oligosaccharides in weaner pigs

1997 ◽  
Vol 1997 ◽  
pp. 57-59
Author(s):  
J.G.M. Houdijk ◽  
B.A. Williams ◽  
S. Tamminga ◽  
M.W.A. Verstegen
Keyword(s):  
Control Diet ◽  
Gas Production ◽  
Crude Fibre ◽  
In Vitro Fermentation ◽  
Sterile Saline ◽  
Weaner Pigs ◽  
Endogenous Enzymes ◽  
Cheese Cloth

Non-digestible oligosaccharides (NDOs), including fructo-oligosaccharides (FOS) and trans-galacto-oligosaccharides (TOS), are not digested by endogenous enzymes, but may be fermented by bacteria such as lactobacilli and bifidobacteria (Tomomatsu, 1994). Thus, dietary NDOs may alter fermentation characteristics of the microflora of the host. In this experiment, in vivo and in vitro fermentation characteristics of NDOs in weaner pigs were studied, using the cumulative gas production technique (Theodorou et al.,1994).A NDO-free control diet (CON), with oatshuskmeal (OHM) as a fibre source, and this diet with 40 g FOS (FOS40) or TOS (TOS40) per kg (w/w against cellulose) was restrictedly fed for 7 weeks (2.6*Em) to 3 groups of four 35-day old individually housed weaner pigs. Total faeces were collected from day 13-18 to determine crude fibre digestibility. Pigs were fitted with a PVTC-cannula on day 21 (Van Leeuwen et al, 1991). Inocula for gas production were anaerobically prepared from faeces (day 14-16) and ileal chyme (day 33-35, via PVTC-cannula). Samples were diluted (1:4) with sterile saline and filtered through cheese cloth. Five ml filtrate were injected into airtight bottles, containing 80 ml growth medium and no substrate (BLA) or 0.25 g FOS, TOS or OHM as sole energy source.

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