scholarly journals Nisin as a Novel Feed Additive: The Effects on Gut Microbial Modulation and Activity, Histological Parameters, and Growth Performance of Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 101 ◽  
Author(s):  
Bartosz Kierończyk ◽  
Mateusz Rawski ◽  
Zuzanna Mikołajczak ◽  
Sylwester Świątkiewicz ◽  
Damian Józefiak
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Dietary Supplement ◽  
Dose Response ◽  
Broiler Chickens ◽  
Experimental Period ◽  
Feed Additive ◽  
Microbial Activities ◽  
Bacterial Populations ◽  
Bacterial Fermentation

Two independent experiments were performed to evaluate the effect of nisin alone or with monensin on gut microbiota, gut microbial activities, and histomorphology (exp 1) and the effect of nisin application in a dose‒response manner on the growth performance of broiler chickens (exp 2). A total of 900 one-day-old female Ross 308 chicks (400, exp 1; 500, exp 2) were randomly distributed to four groups (exp 1; 10 replicate pens per treatment with 10 birds each), i.e., NA, no additives; MON, monensin (100 ppm); NIS, nisin (2700 IU/kg diet); and MON + NIS, a mixture of monensin (100 ppm) and nisin (2700 IU/kg diet); or 5 treatments (exp 2), NA, no additives; NIS100, nisin (100 IU/kg diet); NIS200, nisin (200 IU/kg diet); NIS400, nisin (400 IU/kg diet); and NIS800, nisin (800 IU/kg diet). Nisin supplementation positively affected the microbiota of the gut by reducing potentially pathogenic bacterial populations in the jejunum and ceca. The bacterial fermentation in the jejunum was significantly lowered by nisin addition. The addition of nisin from 100 IU to 800 IU decreased the FCR value over the entire experimental period. According to the results, nisin can be considered a natural dietary supplement for broiler chickens.

Effects of Moringa Oleifera Leaf Polysaccharide on Growth Performance and Immune Response of Broiler Chickens

2021 ◽  
Author(s):  
Hosameldeen Mohamed Husien ◽  
JunJie Huang ◽  
WeiLong Peng ◽  
ShuMei Zheng ◽  
JinGui Li
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
The Body ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
High Dose ◽  
Tnf Α

Abstract Moringa oleifera (MO) is a widely used as the nutritious and non-traditional feed supplementation containing kinds of bioactive substances. However, the enhancement effect of Moringa oleifera leaf Polysaccharide (MOLP) as a feed additive in broilers growth performance and immunity remains unclear. In this study, MOLP was obtained by water extraction and alcohol precipitation method, then purified with Trichloroacetic acid (TCA) assay. Chickens were randomly divided into 4 groups, to receive different doses of MOLP (0, 0.1, 0.2, 0.4g/kg) in feed for 3 weeks. The body weight gain (BWG) and feed consumption were recorded for feed conversion ratio (FCR) and average daily feed intake (ADFI) calculation. Broiler chickens were sacrificed and sampled on day 14, 21, 28 (D 14, D 21, and D 28) respectively. Serological indicators, including total protein (TP), albumin (ALB), globulin (GLO), and creatinine (CREA) were detected. ELISA kits were applied for detecting the levels of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-2 (IL-2), and tumor necrosis factor (TNF-α). From D 21 to D 28, the results showed that middle dose of MOLP significantly increased BWG and ADFI as well as liver and bursa indexes when compared with the control group. In addition, TP and GLO were also increased (P<0.05). All MOLP treatments enhanced the serum concentrations of IgG and IL-2 (P<0.01). Furthermore, results of quantitative RT-PCR showed that high dose of MOLP treatment significantly increased (P<0.001) the mRNA expression levels of IL-2 and TNF-α of chickens relative to the control group. In conclusion, the results showed that MOLP supplementation contributed to improve growth performance and immune response in broiler chickens, and MOLP could be considered as a promising feed additive.

scholarly journals Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens

2021 ◽  
Vol 8 ◽  
Author(s):  
Teketay Wassie ◽  
Zhuang Lu ◽  
Xinyi Duan ◽  
Chunyan Xie ◽  
Kefyalew Gebeyew ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Marine Algae ◽  
Basal Diet ◽  
Control Group ◽  
Villus Height ◽  
Crypt Depth ◽  
Caecal Microbiota

Marine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects of marine algae Enteromorpha (Ulva) prolifera polysaccharide (EP) supplementation on growth performance, immune response, and caecal microbiota of broiler chickens. A total of 200 1-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with ten replications of ten chickens in each replication. The dietary treatments consisted of the control group (fed basal diet), and EP group (received diet supplemented with 400 mg EP/kg diet). Results showed that chickens fed EP exhibited significantly higher (P &lt; 0.05) body weight and average daily gain than the chicken-fed basal diet. In addition, significantly longer villus height, shorter crypt depth, and higher villus height to crypt depth ratio were observed in the jejunal and ileal tissues of chickens fed EP. EP supplementation upregulated the mRNA expression of NF-κB, TLR4, MyD88, IL-2, IFN-α, and IL-1β in the ileal and jejunal tissues (P &lt; 0.05). Besides, we observed significantly higher (P &lt; 0.05) short-chain volatile fatty acids (SCFAs) levels in the caecal contents of the EP group than in the control group. Furthermore, 16S-rRNA analysis revealed that EP supplementation altered gut microbiota and caused an abundance shift at the phylum and genus level in broiler chicken. Interestingly, we observed an association between microbiota and SCFAs production. Overall, this study demonstrated that supplementation of diet with EP promotes growth performance, improves intestinal immune response and integrity, and modulates the caecal microbiota of broiler chickens. This study highlighted the application of marine algae polysaccharides as an antibiotic alternative for chickens. Furthermore, it provides insight to develop marine algae polysaccharide-based functional food and therapeutic agent.

Effects of Compound Probiotics on Growth Performance, Intestinal Microflora and Immune Function of Broilers

2014 ◽  
Vol 997 ◽  
pp. 178-184
Author(s):  
Hong Ling Wang ◽  
Yu Hong Yang ◽  
Jia Qing Wang ◽  
Zhuo Wang ◽  
Shuang Ma
Keyword(s):  
Immune Function ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Intestinal Microflora ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Group Iii ◽  
Serum Biochemical ◽  
Anti Body

This study aimed to investigate the efficacy of compound probiotics on broiler performance, intestinal microflora, immune function and serum biochemical indicators. Four hundred one-d-old female broiler chickens were randomly divided into 4 groups with 4 replicates in each group and 25 chickens per replication. The chickens in groupI(control group) were fed a basal diet, experimental groups II, III and IVwere fed the basal diet supplemented with 0.1%, 0.2% and 0.4% compound probiotics. The results of this work were as follows: (1) during the experimental period, compared with control group, the average daily in group III was significantly increased (P<0.05), the feed/gain and average daily feed intake were significantly lower (P<0.05); (2) at the age of 14 and 28 days, the number of cecal Lactic acid in group II and III were significantly higher than the control (P<0.05) and the content of E.coli has the opposite results. (3) the anti-body titer in group III was the highest, and significantly higher compared to the control (P<0.05), but there were no significantly differences between group IV and control group (P>0.05). (4) serum biochemical indexes of broilers in group III and IVwere significantly increased than that in control group (P<0.05). In conclusion, the supplementation of 0.2% compound probiotics could promote the growth performance, improve the cecal mico-ecosystem, enhance the anti-body titer and biochemical indexes in serum.

Growth performance, digestibility, haematology, biochemistry, and some humoral immunity blood parameters of broiler chickens fed different levels of Boswellia serrata resin

2018 ◽  
Vol 58 (10) ◽  
pp. 1885 ◽  
Author(s):  
Ali Ridha Mustafa Al-Yasiry ◽  
Bożena Kiczorowska ◽  
Wioletta Samolińska ◽  
Edyta Kowalczuk-Vasilev
Keyword(s):  
Growth Performance ◽  
Humoral Immunity ◽  
Broiler Chickens ◽  
Blood Parameters ◽  
Nutrient Digestibility ◽  
Feed Additive ◽  
Boswellia Serrata ◽  
Blood Profile ◽  
Positive Effects ◽  
Different Levels

The study determined the effect of three different levels of Boswellia serrata tree resin (BSR) supplementation in diets for broiler chickens on the growth performance, selected slaughter analysis, nutrient digestibility, as well as haematology, biochemical and some humoral immunity blood parameters. In total, 200 1-day-old broiler chickens were assigned randomly to four treatments. The broiler chickens were fed diets containing 0 (C), 1.5 (BSR1.5), 2 (BSR2), or 2.5% Boswellia serrata resin (BSR2.5). The BSR treatments improved (P < 0.05) the feed intake and feed conversion ratio at breeding Days 22–35, but did not differentiate the nutrient digestibility in the diets. The best-quality carcass with a high proportion of muscles and low fat content (Control versus BSR diets, linear, P < 0.05) was obtained in broiler chickens fed diets supplemented with 2% and 2.5% BSR. The blood profile was affected by the BSR treatments. The lymphocyte count increased linearly in blood (P < 0.05) along the increasing amounts of BSR. The content of uric acid and aspartate aminotransferase activity in blood plasma decreased upon the BSR supplementation (Control vs BSR diets, linear, P < 0.05; and Control vs BSR diets, linear, P < 0.01, respectively). The resin of Boswellia serrata can be considered as a good botanical feed additive, which can have positive effects on the performance and health of broiler chickens.

scholarly journals Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens

2019 ◽  
Vol 64 (No. 9) ◽  
pp. 387-394
Author(s):  
Roman Svejstil ◽  
Vladimir Plachy ◽  
Miroslav Joch ◽  
Hana Salmonova ◽  
Dagmar Duskova ◽  
...  
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Anaerobic Bacteria ◽  
Clostridium Butyricum ◽  
Feed Additive ◽  
Plate Count ◽  
The European Union ◽  
Plate Count Method

Clostridium butyricum CBM 588 is used as a probiotic in eastern Asian countries and has been recently approved as an animal feed additive in the European Union. The purpose of this study was to evaluate the effect of C. butyricum MIYAIRI 588 (CBM 588) on abundance of selected genera of caecal and crop bacteria, volatile fatty acids and growth performance of broiler chickens. We studied counts of anaerobic bacteria in caeca and crops of broiler chickens by plate-count method and evaluated their growth performance. CBM 588 significantly reduced E. coli counts in caeca of broiler chickens at days 10 and 42 and also enhanced their growth performance. Additionally, it significantly increased the amount of butyrate in the caeca that provides energy to enterocytes, resulting in increased weight gains. Out of the obtained results we conclude that C. butyricum CBM 588 influences caecal microbiota of broiler chickens and positively affects their growth performance.

scholarly journals Effect of oligosaccharides extract from palm kernel expeller on growth performance, gut microbiota and immune response in broiler chickens

2015 ◽  
Vol 94 (10) ◽  
pp. 2414-2420 ◽  
Author(s):  
Siamak Rezaei ◽  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Idrus Zulkifli ◽  
Abdoreza Soleimani Farjam ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Palm Kernel
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