scholarly journals High-Throughput Phenotyping of Seed/Seedling Evaluation Using Digital Image Analysis

Agronomy ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 63 ◽  
Author(s):  
Chongyuan Zhang ◽  
Yongsheng Si ◽  
Jacob Lamkey ◽  
Rick Boydston ◽  
Kimberly Garland-Campbell ◽  
...  
2014 ◽  
Vol 104 (9) ◽  
pp. 985-992 ◽  
Author(s):  
Ethan L. Stewart ◽  
Bruce A. McDonald

Zymoseptoria tritici, causal agent of Septoria tritici blotch on wheat, produces pycnidia in chlorotic and necrotic lesions on infected leaves. A high-throughput phenotyping method was developed based on automated digital image analysis that accurately measures the percentage of leaf area covered by lesions (PLACL) as well as pycnidia size and number. A seedling inoculation assay was conducted using 361 Z. tritici isolates originating from a controlled cross and two different winter wheat cultivars. Pycnidia size and density were found to be quantitative traits that showed a continuous distribution in the progeny. There was a weak correlation between pycnidia density and size (r = −0.27) and between pycnidia density and PLACL (r = 0.37). There were significant differences in PLACL and pycnidia density on resistant and susceptible cultivars. In all, >20% of the offspring exhibited significantly different pycnidia density on the two cultivars, consistent with host specialization. Automated image analysis provided greater accuracy and precision compared with traditional visual estimates of virulence. These results show that digital image analysis provides a powerful tool for measuring differences in quantitative virulence among strains of Z. tritici.


Plant Methods ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Chantal Le Marié ◽  
Norbert Kirchgessner ◽  
Patrick Flütsch ◽  
Johannes Pfeifer ◽  
Achim Walter ◽  
...  

2009 ◽  
Vol 31 (8) ◽  
pp. 1197-1201 ◽  
Author(s):  
Manoharan Shankar ◽  
Ramachandran Priyadharshini ◽  
Paramasamy Gunasekaran

2000 ◽  
Vol 10 (2) ◽  
pp. 7-9
Author(s):  
Yaser Natour ◽  
Christine Sapienza ◽  
Mark Schmalz ◽  
Savita Collins

2019 ◽  
Vol 8 (3) ◽  
pp. 11 ◽  
Author(s):  
Gustav Stålhammar ◽  
Thonnie Rose O. See ◽  
Stephen Phillips ◽  
Stefan Seregard ◽  
Hans E. Grossniklaus

2008 ◽  
Vol 14 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Hiromasa Tanaka ◽  
Gojiro Nakagami ◽  
Hiromi Sanada ◽  
Yunita Sari ◽  
Hiroshi Kobayashi ◽  
...  

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