scholarly journals Effects of Salt Stress on Fruit Antioxidant Capacity of Wild (Solanum chilense) and Domesticated (Solanum lycopersicum var. cerasiforme) Tomatoes

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1481
Author(s):  
Juan Pablo Martínez ◽  
Raúl Fuentes ◽  
Karen Farías ◽  
Carolina Lizana ◽  
Juan Felipe Alfaro ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Capacity ◽  
Solanum Lycopersicum ◽  
Fruit Weight ◽  
Interspecific Crosses ◽  
Antioxidant Power ◽  
Tomato Species ◽  
In The Wild ◽  
Solanum Chilense ◽  
The Impact

The effects of salt on the quality of fruits were investigated in order to compare the impact of salt on key fruit properties of the cultivated domesticated tomato species (Solanum lycopersicum) and its wild halophyte relative Solanum chilense. To this end, cherry tomato plants (S. lycopersicum var. cerasiforme) and from accession LA4107 (S. chilense) were maintained for 112 days in the absence or presence of NaCl (40 and 80 mM) in nutrient solution. Among others, salinity decreased fruit weight and increased total soluble solid (TSS) in S. lycopersicum but not in S. chilense. The fruit antioxidant capacity estimated by ferric reducing antioxidant power (FRAP) analysis was higher in S. chilense than in S. lycopersicum and increased in the former while it decreased in the latter in response to NaCl. Salinity increased the lycopene (LYC) content but decreased ß-carotene (b-CAR) concentration in the fruits of S. lycopersicum, while these compounds were not detected in the wild halophyte S. chilense. The oxidative status of salt-treated fruits was more tightly regulated in S. chilense than in S. lycopersicum. The two considered species, however, possess complementary properties and interspecific crosses may therefore be considered as a promising option for the improvement of salt-stress resistance in tomatoes.

scholarly journals Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey

2018 ◽  
Vol 46 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Ferit CELIK ◽  
Mehmet Ramazan BOZHUYUK ◽  
Sezai ERCISLI ◽  
Muttalip GUNDOGDU
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Fruit Weight ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Power

In present study, fruit weight, fruit firmness, external fruit color, soluble solid content, pH, titratable acidity, vitamin C, total phenolics, total anthocyanins, and antioxidant capacity of eight wild bilberry grown in Coruh valley in northeastern Turkey were determined. The blueberry cv. ‘Bluecrop’ is also included study to make comparison with bilberry genotypes. Antioxidant activity of bilberry and blueberry fruits were comparatively assessed by FRAP (Ferric reducing antioxidant power) assay. Significantly higher content of phenolics and anthocyanins was found in bilberry fruits than in blueberry fruits. However blueberry cv. ‘Bluecrop’ exhibited higher fruit weight and vitamin C content than bilberry fruits. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. ‘Bluecrop’ while it was between 492-563 mg gallic acid equaivalent and 307-342 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of  bilberry accessions. Moreover, wild accessions approximately had 2-3 folds higher antioxidant capacity than cv. ‘Bluecrop’. Results suggest the possibility of improving the bioactive and antioxidant properties of bilberry cultivars based food products by using wild ones in cross breeding. It was also concluded that genotypes significantly affect their bioactive content and consequently the possibility of using wild bilberry fruits as a potential source of natural antioxidants in food industry.

scholarly journals Use of a Biostimulant to Mitigate Salt Stress in Maize Plants

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1755
Author(s):  
Roberto D’Amato ◽  
Daniele Del Buono
Keyword(s):  
Salt Stress ◽  
Crop Yields ◽  
Total Phenolic ◽  
Maize Seedlings ◽  
Fresh Weight ◽  
Antioxidant Power ◽  
Crop Development ◽  
Ferric Reducing Antioxidant Power ◽  
Maize Plants ◽  
The Impact

Salinity is considered among the abiotic stresses most impacting agriculture for its ability to interfere with crop development and quality. For this reason, practices and innovations that could contain the deleterious effects of such stress are of pivotal importance for maintaining acceptable crop yields. In this context, this work has concerned the study of severe salt stress (100 mM NaCl) on maize seedlings and the effects of a plant biostimulant (Megafol–Meg) in helping plants to cope with this adversity. Biomass production, pigments, the content Na+ and K+, the accumulation of hydrogen peroxide (H2O2) and lipid peroxidation products (MDA), total phenolic compounds (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) were investigated in control samples, in samples treated with NaCl alone, and in samples treated with NaCl in combination with the biostimulant. The results showed that the biostimulant significantly mitigated the impact of the salt stress on shoot length and fresh weight, on chlorophyll and carotenoid contents, and reduced the amount of Na+ taken up by the species. Regarding the oxidative status, the biostimulated samples revealed lower amounts of H2O2 and MDA, while maize seedlings grown with NaCl alone exhibited the highest increases in the TPC, ABTS, and FRAP. The explanation for these effects is provided by highlighting the effectiveness of the biostimulant in avoiding Na+ accumulation, which resulted in a lower content of H2O2, MDA, TPC, and antioxidant activity.

scholarly journals Mycorrhizal fungi and microalgae modulate antioxidant capacity of basil plants

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Marieta Hristozkova ◽  
Liliana Gigova ◽  
Maria Geneva ◽  
Ira Stancheva ◽  
Ivanina Vasileva ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Green Algae ◽  
Mycorrhizal Fungi ◽  
Radical Scavenging ◽  
Arbuscular Mycorrhizal ◽  
Enzymatic Antioxidants ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
The Impact

Abstract Mycorrhizal fungi, algae and cyanobacteria are some of the most important soil microorganisms and major components of a sustainable soil-plant system. This study presents for the first time evidence of the impact of green alga and cyanobacterium solely and in combination with arbuscular mycorrhizal fungi (AMF) on plant-antioxidant capacity. In order to provide a better understanding of the impact of AMF and soil microalgae on Ocimum basilicum L. performance, changes in the pattern and activity of the main antioxidant enzymes (AOEs), esterases and non-enzymatic antioxidants including phenols, flavonoids, ascorbate, and α-tocopherols were evaluated. The targeted inoculation of O. basilicum with AMF or algae (alone and in combination) enhanced the antioxidant capacity of the plants and the degree of stimulation varied depending on the treatment. Plants in symbiosis with AMF exhibited the highest antioxidant potential as was indicated by the enhanced functions of all studied leaf AOEs: 1.5-, 2- and more than 10-fold rises of superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione reductase (GR), respectively. The greatest increase in the total esterase activity and concentration of phenols, flavonoids and ascorbate was marked in the plants with simultaneous inoculation of mycorrhizal fungi and the green algae. 2,2-diphenyl-1-pycril-hydrazyl (DPPH) free radical scavenging method and ferric reducing antioxidant power (FRAP) assay proved the increased plant antioxidant capacity after co-colonization of green algae and mycorrhizae.

scholarly journals Evaluation of European Cranberrybush (Viburnum opulus L.) genotypes for agro-morphological, biochemical and bioactive characteristics in Turkey

2017 ◽  
Vol 29 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Nilda Ersoy ◽  
Sezai Ercisli ◽  
Muttalip Gundogdu
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Viburnum Opulus ◽  
Total Anthocyanin ◽  
Antioxidant Power ◽  
Total Anthocyanins ◽  
Ferric Reducing Antioxidant Power

AbstractThe study evaluated some agro-morphological (fruit weight, fruit flesh ratio, fruit skin colour, number of fruits per raceme, number of racemes per bush), biochemical (vitamin C, soluble solids content, organic acids), and bioactive (total phenolics, total anthocyanins, total flavonoids, and antioxidant capacity) characteristics of the fruit of a number of selected European Cranberrybush (Viburnum opulus L.) genotypes grown in the Sivas province of Turkey. To evaluate the antioxidant capacity, ferric reducing antioxidant power (FRAP) assays were performed. The results showed genotype-specific differences in most of the agro-morphological, biochemical and bioactive characteristics. The range of fruit weight, number of fruits per raceme, and number of racemes per bush was between 0.67 and 0.82 g, 40.7 and 57.1, and 470 and 581, respectively. The highest vitamin C and soluble solids contents were found in the fruits of genotypes SIV-9 and SIV-6 as 39 mg per 100 g and 12.6%, respectively. Tartaric acid was the main organic acid in all the genotypes; it ranged from 120 to 144 mg per 100 g of fruit FW. Total phenolic, total anthocyanin and total flavonoid contents ranged from 621 to 987 mg gallic acid equivalents per 100 g, 15 to 51 mg cyanidin-3-rutinoside equivalents per 100 g, and 202 to 318 mg rutin equivalents per 100 g, respectively. Genotype SIV-10 had the highest antioxidant capacity (34.90 μmol Trolox per g, based on FRAP assays). The present study shows the potential of the evaluated European Cranberrybush genotypes for improving the nutritional value through germplasm enhancement programmes.

scholarly journals Some Morphological and Biochemical Characteristics of Wild Grown Caucasian Whortleberry (Vaccinium arctostaphylos L.) Genotypes from Northeastern Turkey

2018 ◽  
Vol 47 (2) ◽  
pp. 378-383 ◽  
Author(s):  
Gursel OZKAN ◽  
Sezai ERCISLI ◽  
Alam ZEB ◽  
Guleray AGAR ◽  
Halil Ibrahim SAGBAS ◽  
...  
Keyword(s):  
Organic Acids ◽  
Antioxidant Capacity ◽  
Fruit Shape ◽  
Fruit Weight ◽  
Harvest Date ◽  
Total Phenolic ◽  
Frap Assay ◽  
Total Anthocyanin ◽  
Antioxidant Power ◽  
Biochemical Characteristics

Some important morphological such as fruit weight, fruit external color, fruit shape, the number of berry per cluster, cluster color, plant crown habit, harvest date and biochemical characteristics including sugars, organic acids, total phenolics, total anthocyanins, and antioxidant capacity of thirteen wild grown Caucasian whortleberry sampled from Coruh valley, in northeastern Turkey, were determined. Antioxidant capacity was measured as FRAP assay (ferric reducing antioxidant power). Significantly differences were found among genotypes for most of the selected morphological and biochemical features. The genotypes had in general black fruit color and round shape. Harvest date varied from 2 August to 12 August. Fruit weight ranged from 0.37 to 1.08 g. Genotypes had only fructose and glucose in their fruits as sugars. Citric acid was dominant organic acids and was found between 8.87 and 11.20 g per kg of fresh fruits and followed by tartaric acid (2.85-3.30 g/kg). Fruits of genotypes exhibited very high total phenolic content, which ranged from 3740 to 5541 µg per g on a fresh weight basis. Total anthocyanin contents were between 81 and 172 µg of delphinidin-3-glucoside equivalent in per g fresh fruit indicating great diversity.

scholarly journals Effect of Heat Treatment on Smoothie Quality by Response Surface Methodology

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 6
Author(s):  
Joaquina Pinheiro ◽  
Diana I. Santos ◽  
Elsa M. Gonçalves ◽  
Marta Abreu ◽  
Margarida Moldão-Martins
Keyword(s):  
Heat Treatment ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Fruits And Vegetables ◽  
Total Phenolics ◽  
Treatment Time ◽  
Antioxidant Power ◽  
Quality Properties ◽  
The Impact

Smoothies are a popular and convenient way for to consume bioactive compounds from fruits and vegetables such as total phenolics, carotenoids and flavonoids, with the preservation treatment being an important action to guarantee the safety and extension of shelf-life. The main goal of this study was to evaluate the impact of heat treatment (HT) on smoothie prepared with “Fuji “apple (41%), pineapple (31%), cabbage (8%), pumpkin (10%) and banana (10%), by response surface methodology (RSM), where the temperature (70–100 °C) and treatment time (0.5–10.5 min), were the dependent variables. After optimization of HT conditions, a validation assay was performed to guarantee the minimal changes on color and reduction of 90% of polyphenoloxidase enzyme (PPO). Antioxidant activity (Ferric reducing antioxidant power assay (FRAP), DPPH, ABTS), total phenolics content (TPC), pH and solids soluble content were also analyzed. Predicted models of color parameters (L*, a*, ºh) and PPO enzymatic activity were found to be significant (p < 0.05) with regression coefficients (R2) of 0.84, 0.86, 0.92 and 0.97, respectively. From the RSM-generated model, the HT conditions that ensure a minimal green loss of smoothie and inactivation of PPO enzyme was at 85 °C over 7 min. In the validation study, these conditions were tested and proved to be sufficient to achieve the main goals. In the heat-treated smoothie, increases in TPC (10%) and antioxidant capacity (ABTS: 50%, DPPH: 17%, FRAP: 13%) were attained. This study demonstrated that RSM was efficient to select the optimal conditions of HT and improve the important quality properties that influence the product quality and the potential consumer’s health (TPC and antioxidant capacity).

Salt stress differently affects growth, water status and antioxidant enzyme activities in Solanum lycopersicum and its wild relative Solanum chilense

2014 ◽  
Vol 62 (5) ◽  
pp. 359 ◽  
Author(s):  
Juan Pablo Martínez ◽  
Alejandro Antúnez ◽  
Héctor Araya ◽  
Ricardo Pertuzé ◽  
Lida Fuentes ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Enzyme ◽  
Solanum Lycopersicum ◽  
Enzyme Activities ◽  
Water Status ◽  
Dry Weight ◽  
Saline Stress ◽  
Antioxidant Enzyme Activities ◽  
Solanum Chilense ◽  
Leaf Succulence

The effect of saline stress (NaCl, 40, 80 and 160 mmol L−1 of NaCl) on growth, plant water status and leaf antioxidant enzyme activities was investigated in a commercial cultivar of cherry tomato (Solanum lycopersicum var. cerasiforme L.) and in a wild-related species collected in a salt-affected area of North Chile (Solanum chilense Dun.). Salt stress was applied in a nutrient solution at the vegetative stage during 40 days. The highest NaCl concentration reduced shoot relative growth, fresh and dry weight and leaf area in the cultivated S. lycopersicum but had less impact on S. chilense. Both species were able to efficiently perform osmotic adjustment but S. chilense also exhibited an increase in leaf succulence. The oxidative stress estimated through malondialdehyde quantification was always higher in the cultivated S. lycopersicum, both in the absence and in the presence of salt. Total superoxide dismutase activity (EC 1.15.1.1) increased in response to the highest dose of NaCl in S. chilense but remained constant in S. lycopersicum. Salinity induced an increase in ascorbate peroxidase (EC 1.11.1.11) in S. chilense but reduced it in S. lycopersicum. It is concluded that S. chilense displays efficient strategies to cope with high NaCl doses and that management of the oxidative status is a key mechanism allowing this species to tolerate salinity.

EFFECTS OF SALINE WATER ON WATER STATUS, YIELD AND FRUIT QUALITY OF WILD (SOLANUM CHILENSE) AND DOMESTICATED (SOLANUM LYCOPERSICUM VAR. CERASIFORME) TOMATOES

2012 ◽  
Vol 48 (4) ◽  
pp. 573-586 ◽  
Author(s):  
JUAN-PABLO MARTÍNEZ ◽  
ALEJANDRO ANTÚNEZ ◽  
RICARDO PERTUZÉ ◽  
MARIA DEL PILAR ACOSTA ◽  
XIMENA PALMA ◽  
...  
Keyword(s):  
Salt Stress ◽  
Solanum Lycopersicum ◽  
Prolonged Exposure ◽  
Saline Water ◽  
Fruit Yield ◽  
Soluble Solids ◽  
Cultivated Plants ◽  
Shoot Biomass ◽  
Leaf Water Content ◽  
Solanum Chilense

SUMMARYFarmers around the world are concerned about the effects of human-induced salinity on crop yield and quality. Therefore, researchers are actively testing wild relatives of cultivated plants to identify candidates to improve crop performance under salt stress. A study was conducted to understand the effects of salt stress (Sodium chloride, NaCl) on cultivated tomato species (Solanum lycopersicum var. cerasiforme L.) and a wild tomato relative (Solanum chilense Dun.) from the Northern part of Chile. Plants were cultivated hydroponically under controlled environmental conditions for 112 days with nutrient solution containing 0 mM (3 dS m−1), 40 mM (6 dS m−1) and 80-mM (9 dS m−1) NaCl. Salt stress reduced the shoot biomass in S. lycopersicum but not in S. chilense. Both species were able to maintain the leaf water content; however, the cultivated S. lycopersicum showed osmotic adjustment, while S. chilense did not. Salt stress reduced the total fruit yield in S. lycopersicum based on a decrease in the mean fruit weight, but it had no impact on the number of fruits per plant. In contrast, salt stress had no significant impact on the fruit yield in S. chilense. Salt stress increased the total soluble solids content in S. lycopersicum and the titratable acidity in S. chilense. It was concluded that S. chilense displays a contrasting behaviour in response to prolonged exposure to moderate salinity compared with S. lycopersicum, and that this related species could be an interesting plant for breeding purposes.

scholarly journals Intraspecific diversity observed in the wild tomato species Solanum chilense in initial immune responses towards a glucan elicitor.

2021 ◽  
Author(s):  
Parvinderdeep S. Kahlon ◽  
Andrea Förner ◽  
Michael Muser ◽  
Mhaned Oubounyt ◽  
Michael Gigl ◽  
...  
Keyword(s):  
Immune Responses ◽  
Resistance Phenotype ◽  
Wild Tomato ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Multiple Components ◽  
In The Wild ◽  
Defence Responses ◽  
Solanum Chilense ◽  
High Diversity

Natural plant populations are highly polymorphic and often show intraspecific quantitative, variation in resistance properties against pathogens. The activation of the underlying defence responses can depend on the perception of conserved pathogen-associated molecular patterns (PAMPs). To dissect and understand such variation, we evaluated the diversity of responses induced by laminarin (representing a general glucan elicitor of plant immune responses) in the wild tomato species Solanum chilense. We confirm considerable overlap of the plant's global transcriptional responses to laminarin and to the oomycete pathogen Phytophthora infestans. We further measured key components of basal defence responses such as reactive oxygen species (ROS) production and levels of diverse phytohormones and their derivatives upon elicitation with laminarin in 83 plants originating from nine natural populations of S. chilense from distinct geographic origin. We found high diversity in these components at basal and elicitor-induced levels. We generated generalised linear mixed models (GLMMs) with these components to explain the observed resistance phenotype against P. infestans in the plants and found that additive effects of multiple components best explain resistance at the species level. For individual components, we observed the strongest positive correlation between the resistance phenotype and ethylene (ET) production upon laminarin elicitation. The strength of this correlation differed between individual populations. Chemical inhibition of ET production in individuals from a population, in which ET production was associated with resistance, provoked more severe disease symptoms. Our findings reveal high diversity in the strength of induced defence responses within a species and in the basal levels of other stress-related phytohormones. We show the involvement of multiple components with a quantitatively different contribution of individual components to resistance in geographically separated populations of S. chilense against P. infestans.

scholarly journals Effect of Freeze Drying and Simulated Gastrointestinal Digestion on Phenolic Metabolites and Antioxidant Property of the Natal Plum (Carissa macrocarpa)

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1420
Author(s):  
Faith Seke ◽  
Vimbainashe E. Manhivi ◽  
Tinotenda Shoko ◽  
Retha M. Slabbert ◽  
Yasmina Sultanbawa ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Array Detector ◽  
Gastrointestinal Digestion ◽  
Liquid Chromatograph ◽  
Antioxidant Power ◽  
Intestinal Phase ◽  
Glucosidase Activity ◽  
Freeze Dried ◽  
Small Intestinal ◽  
The Impact

Natal plums (Carissa macrocarpa) are a natural source of bioactive compounds, particularly anthocyanins, and can be consumed as a snack. This study characterized the impact of freeze drying and in vitro gastrointestinal digestion on the phenolic profile, antioxidant capacity, and α-glucosidase activity of the Natal plum (Carissa macrocarpa). The phenolic compounds were quantified using high performance liquid chromatography coupled to a diode-array detector HPLC-DAD and an ultra-performance liquid chromatograph (UPLC) with a Waters Acquity photodiode array detector (PDA) coupled to a Synapt G2 quadrupole time-of-flight (QTOF) mass spectrometer. Cyanidin-3-O-β-sambubioside (Cy-3-Sa) and cyanidin-3-O-glucoside (Cy-3-G) were the dominant anthocyanins in the fresh and freeze-dried Natal plum powder. Freeze drying did not affect the concentrations of both cyanidin compounds compared to the fresh fruit. Both cyanidin compounds, ellagic acid, catechin, epicatechin syringic acid, caffeic acid, luteolin, and quercetin O-glycoside from the ingested freeze-dried Natal plum powder was quite stable in the gastric phase compared to the small intestinal phase. Cyanidin-3-O-β-sambubioside from the ingested Natal plum powder showed bioaccessibility of 32.2% compared to cyanidin-3-O-glucoside (16.3%). The degradation of anthocyanins increased the bioaccessibility of gallic acid, protocatechuic acid, coumaric acid, and ferulic acid significantly, in the small intestinal digesta. The ferric reducing antioxidant power (FRAP), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) activities, and inhibitory effect of α-glucosidase activity decreased in the small intestinal phase. Indigenous fruits or freeze-dried powders with Cy-3-Sa can be a better source of anthocyanin than Cy-3-G due to higher bioaccessibility in the small intestinal phase.

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