scholarly journals High Throughput Screening and QSAR-3D/CoMFA: Useful Tools to Design Predictive Models of Substrate Specificity for Biocatalysts†

Molecules ◽  
2004 ◽  
Vol 9 (8) ◽  
pp. 673-693 ◽  
Author(s):  
J. Carballeira ◽  
M. Quezada ◽  
E. Álvarez ◽  
J. Sinisterra
Keyword(s):  
Substrate Specificity ◽  
High Throughput ◽  
Predictive Models ◽  
High Throughput Screening

scholarly journals Predictive Models of Prenatal Developmental Toxicity from ToxCast High-Throughput Screening Data

2011 ◽  
Vol 124 (1) ◽  
pp. 109-127 ◽  
Author(s):  
Nisha S. Sipes ◽  
Matthew T. Martin ◽  
David M. Reif ◽  
Nicole C. Kleinstreuer ◽  
Richard S. Judson ◽  
...  
Keyword(s):  
High Throughput ◽  
Predictive Models ◽  
High Throughput Screening ◽  
Developmental Toxicity

scholarly journals High Throughput Centrifugal Adhesion Screening Test for Soft Materials

2021 ◽  
Author(s):  
Yusu Chen ◽  
Qifeng Wang ◽  
Carolyn Mills ◽  
Johanna Kann ◽  
Kenneth Shull ◽  
...  
Keyword(s):  
Material Property ◽  
High Throughput ◽  
Predictive Models ◽  
High Throughput Screening ◽  
Dynamic Range ◽  
Low Cost ◽  
Colorimetric Method ◽  
Science Research ◽  
Soft Materials ◽  
Mechanical Adhesion

<p>High-throughput screening of material mechanical properties has the potential to transform material science research in both aiding in material discovery and developing predictive models. However, the development of these assays is inherently difficult with only a few methods and tools reported, and the mounting demand for enormous material property datasets to develop predictive models is unfulfilled by the limited throughput of the current techniques. In particular, equipment cost and instrument limitations prohibit the widespread generation of large material property datasets. We address this problem by developing a high-throughput colorimetric method for testing mechanical adhesion using a common laboratory centrifuge, multi-well plates and microparticles. The technique uses centrifugation to apply a homogenous mechanical detachment force across the samples in the multi-well plate. We also develop a high-throughput sample deposition method to prepare films with uniform thickness in each well, minimizing well-to-well variability in measurements. Our centrifugal adhesion testing method can differentiate polymer films with variate adhesion strengths and shows excellent agreement with the probe tack adhesion test. To illustrate the throughput and consistency of the overall process, we displayed a pattern on a multi-well plate by depositing two different formulations and performing the centrifugal test. We can achieve a throughput of thousands of samples per run, and it is limited only by the number of wells in the plates. With its simplicity, low cost and large dynamic range, this high-throughput method has the potential to change the landscape of adhesive material characterization.</p>

scholarly journals High Throughput Centrifugal Adhesion Screening Test for Soft Materials

2021 ◽  
Author(s):  
Yusu Chen ◽  
Qifeng Wang ◽  
Carolyn Mills ◽  
Johanna Kann ◽  
Kenneth Shull ◽  
...  
Keyword(s):  
Material Property ◽  
High Throughput ◽  
Predictive Models ◽  
High Throughput Screening ◽  
Dynamic Range ◽  
Low Cost ◽  
Colorimetric Method ◽  
Science Research ◽  
Soft Materials ◽  
Mechanical Adhesion

<p>High-throughput screening of material mechanical properties has the potential to transform material science research in both aiding in material discovery and developing predictive models. However, the development of these assays is inherently difficult with only a few methods and tools reported, and the mounting demand for enormous material property datasets to develop predictive models is unfulfilled by the limited throughput of the current techniques. In particular, equipment cost and instrument limitations prohibit the widespread generation of large material property datasets. We address this problem by developing a high-throughput colorimetric method for testing mechanical adhesion using a common laboratory centrifuge, multi-well plates and microparticles. The technique uses centrifugation to apply a homogenous mechanical detachment force across the samples in the multi-well plate. We also develop a high-throughput sample deposition method to prepare films with uniform thickness in each well, minimizing well-to-well variability in measurements. Our centrifugal adhesion testing method can differentiate polymer films with variate adhesion strengths and shows excellent agreement with the probe tack adhesion test. To illustrate the throughput and consistency of the overall process, we displayed a pattern on a multi-well plate by depositing two different formulations and performing the centrifugal test. We can achieve a throughput of thousands of samples per run, and it is limited only by the number of wells in the plates. With its simplicity, low cost and large dynamic range, this high-throughput method has the potential to change the landscape of adhesive material characterization.</p>

scholarly journals High-Throughput Screening for Substrate Specificity-Adapted Mutants of the Nisin Dehydratase NisB

2020 ◽  
Vol 9 (6) ◽  
pp. 1468-1478 ◽  
Author(s):  
Xinghong Zhao ◽  
Rubén Cebrián ◽  
Yuxin Fu ◽  
Rick Rink ◽  
Tjibbe Bosma ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
High Throughput ◽  
High Throughput Screening

Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening

2009 ◽  
Vol 102 (4) ◽  
pp. 988-994 ◽  
Author(s):  
Sung-Hee Park ◽  
Hyung-Yeon Park ◽  
Jae Kyung Sohng ◽  
Hee Chan Lee ◽  
Kwangkyoung Liou ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Domain Swapping

scholarly journals Predictive Models for Cytochrome P450 Isozymes Based on Quantitative High Throughput Screening Data

2011 ◽  
Vol 51 (10) ◽  
pp. 2474-2481 ◽  
Author(s):  
Hongmao Sun ◽  
Henrike Veith ◽  
Menghang Xia ◽  
Christopher P. Austin ◽  
Ruili Huang
Keyword(s):  
Cytochrome P450 ◽  
High Throughput ◽  
Predictive Models ◽  
High Throughput Screening ◽  
P450 Isozymes ◽  
Cytochrome P450 Isozymes

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts
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