scholarly journals Use of Enzyme Linked Immunosorbent Assay for detection of aflatoxin M1 in milk powder

2012 ◽  
Vol 26 (1) ◽  
pp. 39-42 ◽  
Author(s):  
S. D. Al-Sawaf ◽  
O. A. Abdullah ◽  
O. H. Sheet
Keyword(s):  
Immunosorbent Assay ◽  
Milk Powder ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Quantum bead-based fluorescence-linked immunosorbent assay for ultrasensitive detection of aflatoxin M1 in pasteurized milk, yogurt, and milk powder

2019 ◽  
Vol 102 (5) ◽  
pp. 3985-3993 ◽  
Author(s):  
Yaofeng Zhou ◽  
Sicheng Xiong ◽  
KangKang Zhang ◽  
Lin Feng ◽  
Xuelan Chen ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Milk Powder ◽  
Aflatoxin M1 ◽  
Ultrasensitive Detection ◽  
Pasteurized Milk

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

Determination of trace aflatoxin M1 levels in milk and milk products consumed in Turkey by using enzyme-linked immunosorbent assay

2012 ◽  
Vol 25 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Sezgin Bakırdere ◽  
Tolga Yaroğlu ◽  
Nihan Tırık ◽  
Mehmet Demiröz ◽  
Abdullah Karaca
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Products

scholarly journals Determination of Vegetal Proteins in Milk Powder by Enzyme-Linked Immunosorbent Assay: Interlaboratory Study

2002 ◽  
Vol 85 (6) ◽  
pp. 1390-1397 ◽  
Author(s):  
Lourdes Sánchez ◽  
María D Pérez ◽  
Pilar Puyol ◽  
Miguel Calvo ◽  
Gary Brett ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Collaborative Study ◽  
Milk Powder ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Proteins ◽  
Blind Test ◽  
Relative Standard ◽  
Standard Deviations ◽  
Phosphate Buffered Saline

Abstract Eight laboratories participated in a collaborative study to evaluate an enzyme-linked immunosorbent assay (ELISA) to determine soy, pea, and wheat proteins in pasteurized or ultra-high temperature (UHT) milk powders. To perform this assay, polyclonal antibodies for soy, pea, and wheat proteins were obtained from rabbit sera. Collaborators received calibration standards composed of milk powder containing 0–8% (w/w) vegetal protein in total protein and blind test samples containing approximately 1, 2, and 5% (w/w) vegetal protein. An indirect competitive ELISA was performed with a kit prepared by a participating laboratory; the kit contained plates coated with soy, pea, or wheat proteins, the corresponding specific antisera, enzyme-labeled second antibody, and substrate solution. Test samples and calibrants were extracted with phosphate-buffered saline, pH 7.4, containing 0.05% Tween and assayed with the ELISA kits. The degree of adulteration was affected by the type of heat treatment applied to the samples. The estimated percentage of vegetal protein addition was close to the theoretical value for pasteurized samples but much lower for UHT samples. For pasteurized samples, intralaboratory relative standard deviations ranged from 5 to 22% and interlaboratory relative standard deviations ranged from 14 to 34%.

Aflatoxin M1 in Nepalese sera, quantified by combination of monoclonal antibody immunoaffinity chromatography and enzyme-linked immunosorbent assay

1993 ◽  
Vol 14 (6) ◽  
pp. 1233-1235 ◽  
Author(s):  
Hiroki Okumura ◽  
Osamu Kawamura ◽  
Seishi Kishimoto ◽  
Akihiro Hasegawa ◽  
Santosh M. Shrestha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Immunoaffinity Chromatography ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay

Enzyme-linked immunosorbent assay for the detection of bovine rennet whey powder in milk powder and buttermilk powder

2008 ◽  
Vol 18 (3) ◽  
pp. 294-302 ◽  
Author(s):  
Maria G.E.G. Bremer ◽  
Anna E.M. Kemmers-Voncken ◽  
Eduard A.M. Boers ◽  
Rob Frankhuizen ◽  
Willem Haasnoot
Keyword(s):  
Immunosorbent Assay ◽  
Milk Powder ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whey Powder ◽  
Buttermilk Powder
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