scholarly journals Seminal Plasma Cytokines Are Predictive of the Outcome of Boar Sperm Preservation

2019 ◽  
Vol 6 ◽  
Author(s):  
Isabel Barranco ◽  
Lorena Padilla ◽  
Cristina Pérez-Patiño ◽  
Juan M. Vazquez ◽  
Emilio A. Martínez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Plasma Cytokines

scholarly journals Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing1,2

2016 ◽  
Vol 94 (5) ◽  
pp. 1906-1912 ◽  
Author(s):  
M. A. Torres ◽  
G. M. Ravagnani ◽  
D. F. Leal ◽  
S. M. M. K. Martins ◽  
B. B. D. Muro ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
The Stability

Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

2018 ◽  
Vol 198 ◽  
pp. 20-26 ◽  
Author(s):  
D.F. Leal ◽  
M.A. Torres ◽  
G.M. Ravagnani ◽  
S.M.M.K. Martins ◽  
F.V. Meirelles ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Quality Characteristics ◽  
Liquid Storage ◽  
Boar Sperm

scholarly journals Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site

2020 ◽  
Vol 21 (17) ◽  
pp. 6060 ◽  
Author(s):  
Chiara Luongo ◽  
Leopoldo González-Brusi ◽  
Paula Cots-Rodríguez ◽  
Mª José Izquierdo-Rico ◽  
Manuel Avilés ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Proteome Analysis ◽  
Interacting Proteins ◽  
Sperm Transport ◽  
Two Fluids ◽  
Boar Sperm ◽  
Uterine Fluid ◽  
Proteome Changes ◽  
Oviductal Fluid

Ejaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; (2) UF: sperm + 20% UF; (3) OF: sperm + 20% OF; (4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena.

scholarly journals Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

2018 ◽  
Vol 195 ◽  
pp. 30-37 ◽  
Author(s):  
Junwei Li ◽  
Jordi Roca ◽  
Cristina Pérez-Patiño ◽  
Isabel Barranco ◽  
Emilio A. Martinez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm

scholarly journals An Efficient Approach for RNA Extraction from Boar Sperm and Seminal Plasma

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (13) ◽  
Author(s):  
Vanmathy Kasimanickam ◽  
Ramanathan Kasimanickam
Keyword(s):  
Seminal Plasma ◽  
Rna Extraction ◽  
Efficient Approach ◽  
Boar Sperm

scholarly journals Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160988 ◽  
Author(s):  
Mariana Andrade Torres ◽  
Rommy Díaz ◽  
Rodrigo Boguen ◽  
Simone Maria Massami Kitamura Martins ◽  
Gisele Mouro Ravagnani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Seminal Plasma ◽  
Sperm Viability ◽  
Boar Sperm

Relative content of Niemann-Pick C2 protein (NPC2) in seminal plasma, but not that of spermadhesin AQN-1, is related to boar sperm cryotolerance

2020 ◽  
Vol 145 ◽  
pp. 181-189
Author(s):  
Julian Valencia ◽  
Marc Yeste ◽  
Armando Quintero-Moreno ◽  
Carolina del Pilar Niño-Cardenas ◽  
Francisco Javier Henao
Keyword(s):  
Seminal Plasma ◽  
Relative Content ◽  
Boar Sperm ◽  
Niemann Pick

scholarly journals Effects of supplemental seminal plasma on cryopreserved boar sperm quality

2018 ◽  
Vol 48 (1) ◽  
pp. 67
Author(s):  
M Barrientos-Morales ◽  
M Barrientos-Villeda ◽  
B Domínguez-Mancera ◽  
J.M. Pinos-Rodríguez ◽  
M.L. Juárez-Mosqueda
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Boar Sperm
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