scholarly journals Impaired Sensory Processing During Low-Oxygen Exposure: A Noninvasive Approach to Detecting Changes in Cognitive States

2020 ◽  
Vol 11 ◽  
Author(s):  
Todd R. Seech ◽  
Matthew E. Funke ◽  
Richard F. Sharp ◽  
Gregory A. Light ◽  
Kara J. Blacker
Keyword(s):  
Sensory Processing ◽  
Low Oxygen ◽  
Oxygen Exposure ◽  
Cognitive States

scholarly journals Cell-Wide Responses to Low-Oxygen Exposure in Desulfovibrio vulgaris Hildenborough

2007 ◽  
Vol 189 (16) ◽  
pp. 5996-6010 ◽  
Author(s):  
Aindrila Mukhopadhyay ◽  
Alyssa M. Redding ◽  
Marcin P. Joachimiak ◽  
Adam P. Arkin ◽  
Sharon E. Borglin ◽  
...  
Keyword(s):  
Growth Rate ◽  
Stress Response ◽  
Desulfovibrio Vulgaris ◽  
Proteomics Data ◽  
Low Oxygen ◽  
Air Exposure ◽  
Sulfate Reducing ◽  
Oxygen Exposure ◽  
Tetraheme Cytochrome ◽  
Periodic Exposure

ABSTRACT The responses of the anaerobic, sulfate-reducing organism Desulfovibrio vulgaris Hildenborough to low-oxygen exposure (0.1% O2) were monitored via transcriptomics and proteomics. Exposure to 0.1% O2 caused a decrease in the growth rate without affecting viability. Concerted upregulation of the predicted peroxide stress response regulon (PerR) genes was observed in response to the 0.1% O2 exposure. Several of the candidates also showed increases in protein abundance. Among the remaining small number of transcript changes was the upregulation of the predicted transmembrane tetraheme cytochrome c 3 complex. Other known oxidative stress response candidates remained unchanged during the low-O2 exposure. To fully understand the results of the 0.1% O2 exposure, transcriptomics and proteomics data were collected for exposure to air using a similar experimental protocol. In contrast to the 0.1% O2 exposure, air exposure was detrimental to both the growth rate and viability and caused dramatic changes at both the transcriptome and proteome levels. Interestingly, the transcripts of the predicted PerR regulon genes were downregulated during air exposure. Our results highlight the differences in the cell-wide responses to low and high O2 levels in D. vulgaris and suggest that while exposure to air is highly detrimental to D. vulgaris, this bacterium can successfully cope with periodic exposure to low O2 levels in its environment.

scholarly journals Preventing neutrophil from oxygen exposure allows their basal state maintenance

2020 ◽  
Author(s):  
Louise Injarabian ◽  
Quentin Giai Gianetto ◽  
Véronique Witko-Sarsat ◽  
Benoit S Marteyn
Keyword(s):  
Cholesterol Metabolism ◽  
Atmospheric Oxygen ◽  
White Blood Cells ◽  
Coagulation Cascade ◽  
Large Set ◽  
Low Oxygen ◽  
Autologous Plasma ◽  
Cytosolic Proteins ◽  
Oxygen Exposure ◽  
The Impact

AbstractNeutrophils are the most abundant circulating white blood cells and are central players of the innate immune response. During their lifecycle, neutrophils mainly evolve under low oxygen conditions (0.1 - 4% O2) to which they are well adapted. Neutrophils are atypical cells since they are mainly glycolytic, and highly susceptible to oxygen-exposure, which induces their activation and death, through mechanisms which remain currently elusive. Nevertheless, nearly all studies conducted on neutrophils are carried out under atmospheric oxygen (21%), corresponding to hyperoxic conditions. Here we investigated the impact of hyperoxia during neutrophil purification and culture on neutrophil viability, activation and cytosolic protein content. Neutrophil hyper-activation (CD62L shedding) is induced during culture under hyperoxic conditions (24h), compared to neutrophils cultured under anoxic conditions. In addition, we show that maintaining neutrophils in autologous plasma is the most suitable strategy to maintain their basal state.Our results show that manipulating neutrophils under hyperoxic conditions leads to the loss of ~100 cytosolic proteins during purification, while it does not lead to an immediate impact on neutrophils activation (CD11bhigh, CD54high, CD62Llow) or viability (DAPI+). We identified two clusters of proteins belonging to the cholesterol metabolism and to the complement and coagulation cascade pathways, which are highly susceptible to neutrophil oxygen-exposure during their purification.In conclusion, preserving neutrophil from oxygen-exposure during their manipulation – purification and culture- is recommended to avoid their experimental activation and for preserving a large set of cytosolic proteins from alteration.

scholarly journals Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis

2019 ◽  
Author(s):  
Shelly A. Trigg ◽  
Paul McElhany ◽  
Michael Maher ◽  
Danielle Perez ◽  
D. Shallin Busch ◽  
...  
Keyword(s):  
Glycogen Storage ◽  
Low Ph ◽  
Global Ocean ◽  
Dungeness Crab ◽  
Low Oxygen ◽  
Cancer Magister ◽  
Important Species ◽  
Atp Production ◽  
Oxygen Exposure ◽  
Exposure Pathway

ABSTRACTThe Dungeness crab is an economically and ecologically important species distributed along the North American Pacific coast. To predict how Dungeness crab may physiologically respond to future global ocean change on a molecular level, we performed untargeted metabolomic approaches on individual Dungeness crab juveniles reared in treatments that mimicked current and projected future pH and dissolved oxygen conditions. We found 94 metabolites and 127 lipids responded in a condition-specific manner, with a greater number of known compounds more strongly responding to low oxygen than low pH exposure. Pathway analysis of these compounds revealed that juveniles may respond to low oxygen through evolutionarily conserved processes including downregulating glutathione biosynthesis and upregulating glycogen storage, and may respond to low pH by increasing ATP production. Most interestingly, we found that the response of juveniles to combined low pH and low oxygen exposure was most similar to the low oxygen exposure response, indicating low oxygen may drive the physiology of juvenile crabs more than pH. Our study elucidates metabolic dynamics that expand our overall understanding of how the species might respond to future ocean conditions and provides a comprehensive dataset that could be used in future ocean acidification response studies.

Low oxygen exposure does not cause pulmonary injury in the newborn rat

2006 ◽  
Vol 82 (5) ◽  
pp. 335-340 ◽  
Author(s):  
A. White ◽  
J.R. McColm ◽  
J. Wade ◽  
Z. Yaqoob ◽  
K. Sedowofia ◽  
...  
Keyword(s):  
Pulmonary Injury ◽  
Low Oxygen ◽  
Newborn Rat ◽  
Oxygen Exposure

scholarly journals Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids

2021 ◽  
Vol 22 (2) ◽  
pp. 662
Author(s):  
Monia Souidi ◽  
Yvonne Sleiman ◽  
Ivana Acimovic ◽  
Jan Pribyl ◽  
Azzouz Charrabi ◽  
...  
Keyword(s):  
Embryoid Body ◽  
Mitochondrial Respiratory Chain ◽  
Cardiac Differentiation ◽  
Low Oxygen ◽  
Respiratory Chain Complexes ◽  
Oxygen Exposure ◽  
Chain Complexes ◽  
Functional Consequences ◽  
Metabolic Properties ◽  
2D And 3D

Numerous protocols of cardiac differentiation have been established by essentially focusing on specific growth factors on human pluripotent stem cell (hPSC) differentiation efficiency. However, the optimal environmental factors to obtain cardiac myocytes in network are still unclear. The mesoderm germ layer differentiation is known to be enhanced by low oxygen exposure. Here, we hypothesized that low oxygen exposure enhances the molecular and functional maturity of the cardiomyocytes. We aimed at comparing the molecular and functional consequences of low (5% O2 or LOE) and high oxygen exposure (21% O2 or HOE) on cardiac differentiation of hPSCs in 2D- and 3D-based protocols. hPSC-CMs were differentiated through both the 2D (monolayer) and 3D (embryoid body) protocols using several lines. Cardiac marker expression and cell morphology were assessed. The mitochondrial localization and metabolic properties were evaluated. The intracellular Ca2+ handling and contractile properties were also monitored. The 2D cardiac monolayer can only be differentiated in HOE. The 3D cardiac spheroids containing hPSC-CMs in LOE further exhibited cardiac markers, hypertrophy, steadier SR Ca2+ release properties revealing a better SR Ca2+ handling, and enhanced contractile force. Preserved distribution of mitochondria and similar oxygen consumption by the mitochondrial respiratory chain complexes were also observed. Our results brought evidences that LOE is moderately beneficial for the 3D cardiac spheroids with hPSC-CMs exhibiting further maturity. In contrast, the 2D cardiac monolayers strictly require HOE.

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