scholarly journals Direct Germline Transformation of Cotton Meristem Explants With No Selection

2020 ◽  
Vol 11 ◽  
Author(s):  
Yurong Chen ◽  
Andrea Lange ◽  
Zarir Vaghchhipawala ◽  
Xudong Ye ◽  
Annie Saltarikos
Keyword(s):  
Germline Transformation ◽  
Meristem Explants

scholarly journals Germline Transformation of the Silkworm Bombyx mori L. by Sperm-Mediated Gene Transfer1

2012 ◽  
Vol 87 (6) ◽  
Author(s):  
Wenlin Zhou ◽  
Chengliang Gong ◽  
Renyu Xue ◽  
Guangli Cao ◽  
Jinru Cao ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Germline Transformation ◽  
Bombyx Mori L ◽  
Silkworm Bombyx Mori

scholarly journals Establishment of a Rapid Plant Regeneration System in Physalis angulata L. through Axillary Meristems

2015 ◽  
Vol 7 (4) ◽  
pp. 471-474
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Shoot Culture ◽  
Shoot Multiplication ◽  
Multiple Shoot ◽  
Herbaceous Plant ◽  
Axillary Meristem ◽  
Regeneration System ◽  
Axillary Meristems ◽  
Meristem Explants ◽  
Physalis Angulata

An optimal plant propagation method of Physalis angulata L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum in vitro response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA3) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective in vitro shoot elongation and rooting was achieved on 1.0mg/L(GA3) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent.  This protocol can be used for commercial propagation and for future genetic improvement studies.

scholarly journals Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

2017 ◽  
Vol 26 (4) ◽  
pp. 440-452 ◽  
Author(s):  
F. Chu ◽  
W. Klobasa ◽  
P. Wu ◽  
S. Pinzi ◽  
N. Grubbs ◽  
...  
Keyword(s):  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Germline Transformation
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