scholarly journals Integrated Karyotypes of Diploid and Tetraploid Carrizo Citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.) as Determined by Sequential Multicolor Fluorescence in situ Hybridization With Tandemly Repeated DNA Sequences

2020 ◽  
Vol 11 ◽  
Author(s):  
Honghong Deng ◽  
Guohao Tang ◽  
Nuo Xu ◽  
Zhijian Gao ◽  
Lijin Lin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Citrus Sinensis ◽  
Poncirus Trifoliata ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Multicolor Fluorescence ◽  
Tandemly Repeated Dna

The molecular structure, chromosomal organization, and interspecies distribution of a family of tandemly repeated DNA sequences of Antirrhinum majus L.

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Thomas Schmidt ◽  
Jörg Kudla
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Antirrhinum Majus ◽  
Repeated Dna ◽  
Satellite Dnas ◽  
Repeated Dna Sequences ◽  
The North ◽  
Tandemly Repeated Dna

Monomers of a major family of tandemly repeated DNA sequences of Antirrhinum majus have been cloned and characterized. The repeats are 163–167 bp long, contain on average 60% A + T residues, and are organized in head-to-tail orientation. According to site-specific methylation differences two subsets of repeating units can be distinguished. Fluorescent in situ hybridization revealed that the repeats are localized at centromeric regions of six of the eight chromosome pairs of A. majus with substantial differences in array size. The monomeric unit shows no homologies to other plant satellite DNAs. The repeat exists in a similar copy number and conserved size in the genomes of six European species of the genus Antirrhinum. Tandemly repeated DNA sequences with homology to the cloned monomer were also found in the North American section Saerorhinum, indicating that this satellite DNA might be of ancient origin and was probably already present in the ancestral genome of both sections. Key words : Antirrhinum majus, satellite DNA, repetitive DNA, methylation, in situ hybridization.

Genomic organization, sequence interrelationship, and physical localization using in situ hybridization of two tandemly repeated DNA sequences in the genus Olea

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 527-534 ◽  
Author(s):  
Andreas Katsiotis ◽  
Marianna Hagidimitriou ◽  
Alexandra Douka ◽  
Polydefkis Hatzopoulos
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Genomic Library ◽  
Repeat Sequence ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Young Leaves ◽  
Partial Genomic Library ◽  
Tandemly Repeated Dna

Two tandemly repeated DNA sequences, the 81-bp family and pOS218, have been isolated from a Sau3AI Olea europaea ssp. sativa partial genomic library. Sequencing of the 81-bp element showed the monomer to be between 78 and 84 bases long and to contain 51-58% adenine and thymidine residues. Comparison between the monomers revealed heterogeneity of the sequence primary structure. The clone pOS218 is 218 bases long, and sequence comparison between the two elements revealed that an internal region of the pOS218 repeated DNA sequence had 79% homology to the 81 bp repeat sequence. A breakage-reunion mechanism, involving the CAAAA sequence, could be responsible for the derivation of pOS218 from the 81 bp family element. By using double target in situ hybridization, co-localization of the two sequences on Olea chromosomes was observed. The sequences were present at DAPI stained heterochromatic regions, as major or minor sites having a subtelomeric or interstitial location. Methylation studies using two sets of isoschizomers, Sau3AI-MboI and MspI-HpaII, demonstrated that most cytosine residues in the GATC sites and the internal cytosine in the CCGG sites of both elements were methylated in O. europaea ssp. sativa. No major difference in methylation was apparent between DNA extracted from young leaves or from callus of O. europaea ssp.sativa. Both elements are also present in Olea chrysophylla, Olea oleaster, and Olea africana, but are absent from other Oleaceae genera, including Phillyrea, Forsythia, Ligustrum, Parasyringa, and Jasminum.Key words: in situ hybridization, methylation, Oleaceae, phylogenetic relationships, repeated sequences.

Distribution of highly repeated DNA sequences in species of the genus Secale

Genome ◽  
1997 ◽  
Vol 40 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Angeles Cuadrado ◽  
Nicolás Jouve
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Nucleotide Sequences ◽  
Repeated Dna ◽  
Accurate Identification ◽  
Highly Repetitive Dna

The presence and distribution of the most important highly repetitive DNA sequences of rye in cultivated and wild species of the genus Secale were investigated using fluorescence in situ hybridization. Accurate identification of individual chromosomes in the most commonly recognized species or subspecies of the genus Secale (S. cereale, S. ancestrale, S. segetale, S. afghanicum, S. dighoricum, S. montanum, S. montanum ssp. kuprijanovii, S. africanum, S. anatolicum, S. vavilovii, and S. silvestre) was achieved using three highly repetitive rye DNA sequences (probes pSc119.2, pSc74, and pSc34) and the 5S ribosomal DNA sequence pTa794. It is difficult to superimpose trends in the complexity of repetitive DNA during the evolution of the genus on conclusions from other cytogenetic and morphological assays. However, there are two clear groups. The first comprises the self-pollinated annuals S. silvestre and S. vavilovii that have few repeated nucleotide sequences of the main families of 120 and 480 bp. The second group presents amplification and interstitialization of the repeated nucleotide sequences and includes the perennials S. montanum, S. anatolicum, S. africanum, and S. kuprijanovii, as well as the annual and open-pollinated species S. cereale and its related weedy forms. The appearance of a new locus for 5S rRNA in S. cereale and S. ancestrale suggests that cultivated ryes evolved from this wild weedy species.Key words: rye, repeated nucleotide sequence, 5S rDNA, fluorescence in situ hybridization, FISH.

Organization of highly repeated sequences in surface-spread pachytene chromosomes of rye

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 945-948 ◽  
Author(s):  
N Cuñado ◽  
J Barrios ◽  
J L Santos
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Repeated Dna ◽  
Telomeric Sequences ◽  
Different Types ◽  
Surface Spread ◽  
Fish Signal

A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescence in situ hybridization (FISH) has been applied to analyze the location and organization of five different highly repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin displays different types of organization. Telomeric sequences were seen tightly associated with the SC while other repetitive DNA sequences were found to form loops that are associated with SCs only at their bases. On the contrary, the FISH signal of a centromeric satellite had a granular appearance, reflecting that the hybridization occurs only with parts of the chromatin loops.Key words: fluorescence in situ hybridization, meiosis, repetitive DNA, rye, synaptonemal complex.

Molecular characterization and chromosome location of repeated DNA sequences in Hordeum species and in the amphiploid tritordeum (×Tritordeum Ascherson et Graebner)

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 850-857 ◽  
Author(s):  
Esther Ferrer ◽  
Yolanda Loarce ◽  
Gregorio Hueros
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Diploid Species ◽  
Repeated Sequences ◽  
Hordeum Chilense ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
In Situ Experiments ◽  
Basic Genome

Genomic DNA from 19 species and subspecies representing the four basic genomes (H, I, X, and Y) of Hordeum was restricted with HaeIII and hybridized with two repeated DNA sequences of Hordeum chilense. The potential use of repeated sequences in ascertaining genomic affinities within the genus Hordeum was studied by comparing restriction fragment patterns. The study demonstrated the following: (i) species that shared a basic genome showed more similar hybridization fragment patterns than species with different genomes, whether with pHchl or pHch3; (ii) hybridization with pHchl revealed the presence of certain fragments limited to the species with a H genome; and (iii) the alloploid nature of species like H. jubatum was confirmed. The chromosomal distribution of the two repeated sequences was studied in species representing each basic genome and in the amphiploid tritordeum using fluorescent in situ hybridization. No interspecific differences were found between the diploid species. In situ experiments indicated the alloploid nature of H. depressum. Both sequences allow H. chilense chromatin to be distinguished from wheat chromosomes in tritordeum.Key words: repeated DNA sequences; in situ hybridization, Hordeum, tritordeum.

Identification and chromosomal location of a new tandemly repeated DNA in maize

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 181-184 ◽  
Author(s):  
Chunxian Chen ◽  
Huihuang Yan ◽  
Wenxue Zhai ◽  
Lihuang Zhu ◽  
Jingsan Sun
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Fluorescent In Situ Hybridization ◽  
Methylation Pattern ◽  
Repeated Dna ◽  
Dna Library ◽  
New Family ◽  
Genomic Dna Library ◽  
Tandemly Repeated Dna

Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6. Key words: Zea mays, tandemly repeated DNA, satellite DNA, fluorescent in situ hybridization (FISH).

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences
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