scholarly journals Human Pathogen Colonization of Lettuce Dependent Upon Plant Genotype and Defense Response Activation

2020 ◽  
Vol 10 ◽  
Author(s):  
Cristián Jacob ◽  
Maeli Melotto
2008 ◽  
Vol 55 (6) ◽  
pp. 748-755 ◽  
Author(s):  
E. V. Persiyanova ◽  
K. V. Kiselev ◽  
V. P. Bulgakov ◽  
N. F. Timchenko ◽  
G. K. Chernoded ◽  
...  

2006 ◽  
Author(s):  
Dov Prusky ◽  
Lisa Vaillancourt ◽  
Robert Fluhr

Postharvest decay of fruits and vegetables caused by pathogenic and saprophytic fungi significantly impairs the quality and quantity of fresh produce brought to market. Consequently, there is considerable interest in identifying factors that determine the susceptibility of these commodities to pathogen infection. Insidious postharvest decays remain quiescent during fruit growth and harvest, but activate during the postharvest period. A key response to the physiological changes occurring during fruit ripening is the initiation of ammonium secretion by the pathogen. Ammonium ions at the infection site (ammonification) have subsequent effects on both the pathogen and the host. An accompanying alkalinization process resulting from ammonia accumulation contributes to pathogenicity, since some important fungal virulence factors, (such as pectate lyase in Colletotrichum sp.), are significantly expressed only under alkaline conditions. In this proposal, investigated the mechanisms by which ammonification and alkalinization of infected tissues by the pathogen affect the host’s defense response to fungal attack, and instead increase compatibility during postharvest pathogen-host interactions. Our hypotheses were:1) that host signals, including ripening-related changes, induce secretion of ammonia by the pathogen; 2) that ammonia accumulation, and the resultant environmental alkalinization regulate the expression of fungal virulence genes that are essential for postharvest rot development; 3) that ammonification enhanced fungal colonization, by “suppression of host responses”, including production of reactive oxygen species, activation of superoxide, and polyphenol oxidase production. Our objectives were: to analyze: 1) factor(s) which activate the production and secretion of ammonia by the fungus; 2) fungal gene(s) that play role(s) in the ammonification process; 3) the relationship between ammonification and the activation of host defense response(s) during pathogen colonization; and 4) analyze hostgene expression in alkalinized regions of fruits attacked by hemibiotrophic fungi.


1996 ◽  
Author(s):  
Jaime Vila ◽  
M. Carmen Fernandez ◽  
M. Nieves Perez ◽  
Gustavo Reyes
Keyword(s):  

Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.


2012 ◽  
Vol 37 (11) ◽  
pp. 2106-2110 ◽  
Author(s):  
Dan ZHAO ◽  
Ji-Rong ZHAO ◽  
Xi HUANG ◽  
Ning LI ◽  
Yan LIU ◽  
...  

2015 ◽  
Vol 22 (18) ◽  
pp. 2225-2235 ◽  
Author(s):  
M.H. Branquinha ◽  
S.S.C. Oliveira ◽  
L.S. Sangenito ◽  
C.L. Sodre ◽  
L.F. Kneipp ◽  
...  

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