scholarly journals The Calcium-Dependent Protein Kinase CPK33 Mediates Strigolactone-Induced Stomatal Closure in Arabidopsis thaliana

2019 ◽  
Vol 10 ◽  
Author(s):  
Xuening Wang ◽  
Shuo Lv ◽  
Xiangyu Han ◽  
Xiongjuan Guan ◽  
Xiong Shi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Kinase ◽  
Stomatal Closure ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

scholarly journals Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yi-Ju Lu ◽  
Pai Li ◽  
Masaki Shimono ◽  
Alex Corrion ◽  
Takumi Higaki ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Biology ◽  
Stomatal Closure ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Cytoskeleton Organization ◽  
Effector Triggered Immunity ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

AbstractPattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity.

Expression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana

1996 ◽  
Vol 30 (6) ◽  
pp. 1259-1275 ◽  
Author(s):  
Yan Hong ◽  
Makoto Takano ◽  
Chun-Ming Liu ◽  
Alexander Gasch ◽  
Mee-Len Chye ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Kinase ◽  
Gene Family ◽  
Dependent Protein Kinase ◽  
Kinase Gene ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Protein Kinase Gene ◽  
Calcium Dependent Protein Kinase

scholarly journals Calcium-Dependent Protein Kinase CPK21 Functions in Abiotic Stress Response in Arabidopsis thaliana

2011 ◽  
Vol 4 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Sandra Franz ◽  
Britta Ehlert ◽  
Anja Liese ◽  
Joachim Kurth ◽  
Anne-Claire Cazalé ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Kinase ◽  
Abiotic Stress Response ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

scholarly journals A Novel Calcium-Dependent Protein Kinase 1 Inhibitor Potently Prevents Toxoplasma gondii Transmission to Foetuses in Mouse

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4203
Author(s):  
Héloïse Débare ◽  
Nathalie Moiré ◽  
Firmin Baron ◽  
Louis Lantier ◽  
Bruno Héraut ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Toxoplasma Gondii ◽  
Intraperitoneal Administration ◽  
Congenital Toxoplasmosis ◽  
Parasite Burden ◽  
Oral Route ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

Treatments currently used to prevent congenital toxoplasmosis are non-specific of Toxoplasma gondii and have grievous side effects. To develop a more specific and less toxic drug, we have designed SP230, an imidazo[1,2-b]pyridazine salt targeting the Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) and active against acute toxoplasmosis in mice. Efficiency of SP230 to inhibit foetal transmission of the parasite was evaluated in a mouse model of congenital toxoplasmosis. Swiss mice were infected at mid-pregnancy with tachyzoites or cysts of the ME49 strain of T. gondii by intraperitoneal and oral route, respectively, and treated with SP230 at 50 mg/kg for 5 days by the same routes. Parasite burden in organs of dams and in foetuses was measured by quantitative PCR. Intraperitoneal administration of SP230 drastically reduced the number of parasites (more than 97% of reduction) in the brain and lungs of dams, and led to a reduction of 66% of parasite burden in foetuses. Oral administration of SP230 was particularly efficient with 97% of reduction of parasite burdens in foetuses. SP230 did not impact number and weight of offspring in our conditions. This inhibitor of TgCDPK1 is a promising candidate for the development of alternative therapeutics to treat infected pregnant women.

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