scholarly journals The Use of Pentaploid Crosses for the Introgression of Amblyopyrum muticum and D-Genome Chromosome Segments Into Durum Wheat

2019 ◽  
Vol 10 ◽  
Author(s):  
Manel Othmeni ◽  
Surbhi Grewal ◽  
Stella Hubbart-Edwards ◽  
Caiyun Yang ◽  
Duncan Scholefield ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Genome Chromosome ◽  
D Genome ◽  
Chromosome Segments

A cytological and molecular analysis of D-genome chromosome retention following F2–F6 generations of hexaploid×tetraploid wheat crosses

2018 ◽  
Vol 69 (2) ◽  
pp. 121 ◽  
Author(s):  
Sriram Padmanaban ◽  
Peng Zhang ◽  
Mark W. Sutherland ◽  
Noel L. Knight ◽  
Anke Martin
Keyword(s):  
Durum Wheat ◽  
Tetraploid Wheat ◽  
Triticum Aestivum L ◽  
Food Crops ◽  
Cytological Analysis ◽  
Genome Chromosome ◽  
D Genome ◽  
Ploidy Levels ◽  
F2 Generation ◽  
Global Food

Both hexaploid bread wheat (AABBDD) (Triticum aestivum L.) and tetraploid durum wheat (AABB) (T. turgidum spp. durum) are highly significant global food crops. Crossing these two wheats with different ploidy levels results in pentaploid (AABBD) F1 lines. This study investigated the differences in the retention of D chromosomes between different hexaploid × tetraploid crosses in subsequent generations by using molecular and cytological techniques. Significant differences (P < 0.05) were observed in the retention of D chromosomes in the F2 generation depending on the parents of the original cross. One of the crosses, 2WE25 × 950329, retained at least one copy of each D chromosome in 48% of its F2 lines. For this cross, the retention or elimination of D chromosomes was determined through several subsequent self-fertilised generations. Cytological analysis indicated that D chromosomes were still being eliminated at the F5 generation, suggesting that in some hexaploid × tetraploid crosses, D chromosomes are unstable for many generations. This study provides information on the variation in D chromosome retention in different hexaploid × tetraploid wheat crosses and suggests efficient strategies for utilising D genome retention or elimination to improve bread and durum wheat, respectively.

Production of durum wheat substitution haploids from durum × maize crosses and their cytological characterization

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 137-142 ◽  
Author(s):  
M Dogramac1-Altuntepe ◽  
P P Jauhar
Keyword(s):  
In Situ Hybridization ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Genomic In Situ Hybridization ◽  
Substitution Lines ◽  
Genome Chromosome ◽  
D Genome ◽  
Maize Pollen ◽  
A Genome

The objective of this study was to investigate the effect of individual durum wheat (Triticum turgidum L.) chromosomes on crossability with maize (Zea mays L.) and to cytologically characterize the haploids recovered. Fourteen 'Langdon' (LDN) D-genome disomic substitution lines, a LDN Ph mutant (Ph1b ph1b), and normal 'Langdon' were pollinated with maize pollen. After pollination, hormonal treatment was given daily for up to 14 days. Haploid embryos were obtained from all lines and were aseptically cultured. From a total of 55 358 pollinated florets, 895 embryos were obtained. Only 14 of the embryos germinated and developed into healthy plants. Different substitution lines showed varying degrees of success. The most successful was the substitution 5D(5B) for both embryo formation and haploid plantlet production. These results indicate that the substitution of 5D for 5B confers on durum wheat a greater ability to produce haploids. Fluorescent genomic in situ hybridization (GISH) showed that the substitution haploids consisted of 7 A-genome chromosomes, 6 B-genome chromosomes, and 1 D-genome chromosome. Triticum urartu Tum. genomic DNA was efficient in probing the 7 A-genome chromosomes, although the D-genome chromosome also showed intermediate hybridization. This shows a close affinity between the A genome and D genome. We also elucidated the evolutionary translocation involving the chromosomes 4A and 7B that occurred at the time of evolution of durum wheat. We found that the distal segment translocated from chromosome 7B constitutes about 24% of the long arm of 4A.Key words: cyclic translocation 4A·5A·7B, crossability, disomic substitution, fluorescent genomic in situ hybridization (GISH), Triticum turgidum.

Aneuploid analyses of hybrid necrosis and hybrid chlorosis in tetraploid wheats using the D genome chromosome substitution lines of durum wheat

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 594-601 ◽  
Author(s):  
Koichiro Tsunewaki
Keyword(s):  
Durum Wheat ◽  
Tetraploid Wheat ◽  
Hybrid Necrosis ◽  
Chromosome Substitution ◽  
Substitution Lines ◽  
Genome Chromosome ◽  
D Genome ◽  
Chromosome Substitution Lines ◽  
Complementary Genes ◽  
Tetraploid Wheats

Chromosomal locations of the Ne1 gene, one of the two complementary genes for type 1 hybrid necrosis, and two complementary genes, Cs1 and Cs2, for type 2 hybrid chlorosis in tetraploid wheats were determined by aneuploid analyses employing the D genome chromosome substitution lines of 'Langdon' durum wheat. The Ne1 gene of 'Langdon' is located on chromosome 5B, whereas the Cs1 gene of Triticum dicoccum 'Hokudai' and the Cs2 gene of T. timopheevi are located on chromosomes 5A and 4G, respectively. Chromosomes 4B and 4G show almost complete functional compensation, though they rarely pair with each other, but chromosome 4D of T. aestivum 'Chinese Spring' has only half the ability of chromosome 4G in compensating for chromosome 4B on the fertilization ability of the male gamete. The results have demonstrated the usefulness of the D genome chromosome substitution lines of durum wheat for determining the chromosomes carrying major genes in tetraploid wheat. The results of these studies support the reallocation of chromosome 4A to the B genome.Key words: durum wheat, hybrid necrosis, hybrid chlorosis, aneuploid analyses, chromosome substitution lines.

Molecular characterization and chromosome-specific TRAP-marker development for Langdon durum D-genome disomic substitution lines

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1545-1554 ◽  
Author(s):  
J. Li ◽  
D.L. Klindworth ◽  
F. Shireen ◽  
X. Cai ◽  
J. Hu ◽  
...  
Keyword(s):  
Triticum Turgidum ◽  
Tetraploid Wheat ◽  
Substitution Lines ◽  
Genome Chromosome ◽  
D Genome ◽  
Single Chromosome ◽  
B Genome ◽  
The Individual ◽  
Trap Marker

The aneuploid stocks of durum wheat ( Triticum turgidum L. subsp. durum (Desf.) Husnot) and common wheat ( T. aestivum L.) have been developed mainly in ‘Langdon’ (LDN) and ‘Chinese Spring’ (CS) cultivars, respectively. The LDN-CS D-genome chromosome disomic substitution (LDN-DS) lines, where a pair of CS D-genome chromosomes substitute for a corresponding homoeologous A- or B-genome chromosome pair of LDN, have been widely used to determine the chromosomal locations of genes in tetraploid wheat. The LDN-DS lines were originally developed by crossing CS nulli-tetrasomics with LDN, followed by 6 backcrosses with LDN. They have subsequently been improved with 5 additional backcrosses with LDN. The objectives of this study were to characterize a set of the 14 most recent LDN-DS lines and to develop chromosome-specific markers, using the newly developed TRAP (target region amplification polymorphism)-marker technique. A total of 307 polymorphic DNA fragments were amplified from LDN and CS, and 302 of them were assigned to individual chromosomes. Most of the markers (95.5%) were present on a single chromosome as chromosome-specific markers, but 4.5% of the markers mapped to 2 or more chromosomes. The number of markers per chromosome varied, from a low of 10 (chromosomes 1A and 6D) to a high of 24 (chromosome 3A). There was an average of 16.6, 16.6, and 15.9 markers per chromosome assigned to the A-, B-, and D-genome chromosomes, respectively, suggesting that TRAP markers were detected at a nearly equal frequency on the 3 genomes. A comparison of the source of the expressed sequence tags (ESTs), used to derive the fixed primers, with the chromosomal location of markers revealed that 15.5% of the TRAP markers were located on the same chromosomes as the ESTs used to generate the fixed primers. A fixed primer designed from an EST mapped on a chromosome or a homoeologous group amplified at least 1 fragment specific to that chromosome or group, suggesting that the fixed primers might generate markers from target regions. TRAP-marker analysis verified the retention of at least 13 pairs of A- or B-genome chromosomes from LDN and 1 pair of D-genome chromosomes from CS in each of the LDN-DS lines. The chromosome-specific markers developed in this study provide an identity for each of the chromosomes, and they will facilitate molecular and genetic characterization of the individual chromosomes, including genetic mapping and gene identification.

The inheritance and chromosomal location of a gene for chocolate chaff in durum wheat

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 229-233 ◽  
Author(s):  
C. F. Konzak ◽  
L. R. Joppa
Keyword(s):  
Durum Wheat ◽  
Chromosomal Location ◽  
Triticum Turgidum ◽  
Recessive Gene ◽  
Single Recessive Gene ◽  
Chromosome Substitution ◽  
Substitution Lines ◽  
D Genome ◽  
B Genome ◽  
Aneuploid Chromosome

The durum wheat (Triticum turgidum L. var. durum) cultivar 'Vic' was treated with the chemical mutagen N-methyl-N′-nitrosourea and among the M2 progeny a mutant with "chocolate chaff" (designated cc) was identified. Genetic analyses indicated that chocolate chaff is due to a single recessive gene mutation. The penetrance of the gene for chocolate chaff was environmentally influenced and varied from dark blotches on the glumes to complete coloration of culms as well as spikes. To determine the chromosomal location of the gene, the mutant was crossed with a set of 'Langdon' durum disomic substitution lines in which each of the 14 A- and B-genome chromosomes of durum wheat were replaced by their respective D-genome homoeologues. The segregation of cc was normal in all of the crosses except for those with the 7D(7A) and 7D(7B) lines. Cytogenetic analysis indicated that the gene was located on chromosome 7B, and that chromosome 7D has a gene that prevents the expression of cc when present in one or more copies. It was shown that the 'Langdon' D-genome disomic substitution lines can be used to determine the chromosomal location of genes in tetraploid wheat.Key words: Triticum turgidum, aneuploid, chromosome substitution, monosomic, cytogenetics.

INHERITANCE OF THE TYPE OF SOLID STEM IN GOLDEN BALL (TRITICUM DURUM): II. CYTOGENETICS OF THE RELATION BETWEEN SOLID STEM AND OTHER MORPHOLOGICAL CHARACTERS IN HEXAPLOID F5 LINES OF A HYBRID WITH RESCUE (T. AESTIVUM)

1959 ◽  
Vol 37 (6) ◽  
pp. 1207-1216 ◽  
Author(s):  
Ruby I. Larson
Keyword(s):  
Triticum Aestivum ◽  
Triticum Durum ◽  
Triticum Aestivum L ◽  
Morphological Characters ◽  
Genome Chromosome ◽  
D Genome ◽  
Stem Solidness ◽  
Pentaploid Hybrid ◽  
Golden Ball ◽  
Solid Stem

Cytogenetic analysis of selected F5 lines of the pentaploid hybrid, Rescue (Triticum aestivum L. emend. Thell.) × Golden Ball (T. durum Desf.) showed that chromosome XVI is the member of the D genome of Rescue that prevents transfer of the more solid top culm internode of Golden Ball to hexaploid segregates. It also produces a lax spike. Chromosome XX, which is the D-genome chromosome mainly responsible for the hollowness of hollow-stemmed hexaploids, probably has little effect in Rescue. Long awns were associated with low chromosome number but not with stem solidness or dense spike; therefore, the chromosome that suppresses awn development is probably not XVI.Three 42-chromosome segregates from the cross were more solid in the top internode than Rescue, presumably because of segregation of genes in the A and B genomes. It is unlikely, however, that a fully hexaploid segregate with a top internode as solid as that of Golden Ball can be selected from this hybrid.

scholarly journals Pyramiding different alien chromosome segments in durum wheat: Feasibility and breeding potential

2007 ◽  
Vol 55 (3) ◽  
pp. 267-276 ◽  
Author(s):  
Andrea Gennaro ◽  
Paola Forte ◽  
Roberta Carozza ◽  
Maria L. Savo Sardaro ◽  
Daniela Ferri ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Alien Chromosome ◽  
Breeding Potential ◽  
Chromosome Segments
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