scholarly journals An Optimized Screen Reduces the Number of GA Transporters and Provides Insights Into Nitrate Transporter 1/Peptide Transporter Family Substrate Determinants

2019 ◽  
Vol 10 ◽  
Author(s):  
Nikolai Wulff ◽  
Heidi Asschenfeldt Ernst ◽  
Morten Egevang Jørgensen ◽  
Sophie Lambertz ◽  
Tobias Maierhofer ◽  
...  
Keyword(s):  
Peptide Transporter ◽  
Nitrate Transporter ◽  
Transporter Family

A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants

2014 ◽  
Vol 19 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Sophie Léran ◽  
Kranthi Varala ◽  
Jean-Christophe Boyer ◽  
Maurizio Chiurazzi ◽  
Nigel Crawford ◽  
...  
Keyword(s):  
Family Members ◽  
Peptide Transporter ◽  
Nitrate Transporter ◽  
Transporter Family

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Mycorrhiza ◽  
2017 ◽  
Vol 28 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Navina Drechsler ◽  
Pierre-Emmanuel Courty ◽  
Daphnée Brulé ◽  
Reinhard Kunze
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Peptide Transporter ◽  
Nitrate Transporter ◽  
Transporter Family

scholarly journals Genome-Wide Identification and Analysis of Apple NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER Family (NPF) Genes Reveals MdNPF6.5 Confers High Capacity for Nitrogen Uptake under Low-Nitrogen Conditions

2018 ◽  
Vol 19 (9) ◽  
pp. 2761 ◽  
Author(s):  
Qian Wang ◽  
Changhai Liu ◽  
Qinglong Dong ◽  
Dong Huang ◽  
Cuiying Li ◽  
...  
Keyword(s):  
Nitrogen Uptake ◽  
Expression Patterns ◽  
High Capacity ◽  
General Information ◽  
Amino Acid Sequences ◽  
Peptide Transporter ◽  
Future Research ◽  
Nitrate Transporter ◽  
Transcriptional Responses ◽  
Transporter Family

The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins play important roles in moving substrates such as nitrate, peptides, amino acids, dicarboxylates, malate, glucosinolates, indole acetic acid (IAA), abscisic acid (ABA), and jasmonic acid. Although a unified nomenclature of NPF members in plants has been reported, this gene family has not been studied as thoroughly in apple (Malus × domestica Borkh.) as it has in other species. Our objective was to provide general information about apple MdNPFs and analyze the transcriptional responses of some members to different levels of nitrate supplies. We identified 73 of these genes from the apple genome and used phylogenetic analysis to organize them into eight major groups. These apple NPFs are structurally conserved, based on alignment of amino acid sequences and analyses of phylogenetics and conserved domains. Examination of their genomic structures indicated that these genes are highly conserved among other species. We monitored 14 cloned MdNPFs that showed varied expression patterns under different nitrate concentrations and in different tissues. Among them, NPF6.5 was significantly induced by both low and high levels of nitrate. When compared with the wild type, 35S:MdNPF6.5 transgenic apple calli were more tolerant to low-N stress, which demonstrated that this gene confers greater capacity for nitrogen uptake under those conditions. We also analyzed the expression patterns of those 73 genes in various tissues. Our findings benefit future research on this family of genes.

scholarly journals NPF genes excavation and their expression response to vernalization and nitrogen deficiency in allotetraploid rapeseed

2021 ◽  
Author(s):  
Hongbo Chao ◽  
Jianjie He ◽  
Weiguo Zhao ◽  
Hong Fu ◽  
Yingpeng Hua ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Functional Characterization ◽  
Nitrogen Deficiency ◽  
Enrichment Analysis ◽  
Functional Differentiation ◽  
Peptide Transporter ◽  
Peptide Transporters ◽  
Transporter Family ◽  
Expression Response ◽  
Metabolite Content

Abstract Background The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) genes, initially characterized as nitrate or peptide transporters in plants, involve in the transport of a large variety of substrates including amino acids, nitrate, auxin (IAA), jasmonates (JAs), abscisic acid (ABA) and gibberellins (GAs) and glucosinolates. The evolution and expression diversification of genes determine their functional differentiation in polyploid species. Results Among 169 NPF genes excavated in Brassica napus, 97 B. napus NPF (BnaNPF) genes evolved from B. rapa, and 72 BnaNPF genes from B. olereaca. They unevenly distributed on B. napus genome and exhibited obvious synteny with NPF genes in Arabidopsis thaliana, B. rapa and B. olereaca. BnaNPF genes were identified to show diversified expression patterns in 90 different organs or tissues based on transcriptome profile data. Besides, they exhibited complex expression changes in the development process of leaves, silique wall and seeds, which indicated that the expression of BnaNPF genes maybe respond to altered phytohormone and secondary metabolite content through combining with promoter elements enrichment analysis. Furthermore, many BnaNPF genes were detected to response to vernalization with two different patterns and 20 BnaNPF genes responded to nitrate deficiency. Conclusion The evolution of BnaNPF genes and their expression pattern including response to vernalization and nitrogen deficiency were characterized and provide valuable information for further functional characterization in rapeseed.

Genome-wide identification and expression analyses of nitrate transporter family genes in wild soybean (Glycine soja)

2020 ◽  
Vol 61 (4) ◽  
pp. 489-501
Author(s):  
Hongguang You ◽  
Yuanming Liu ◽  
Thuy Nguyen Minh ◽  
Haoran Lu ◽  
Pengmin Zhang ◽  
...  
Keyword(s):  
Glycine Soja ◽  
Wild Soybean ◽  
Nitrate Transporter ◽  
Transporter Family ◽  
Genome Wide

scholarly journals The Nitrate Transporter Family Protein LjNPF8.6 Controls the N-Fixing Nodule Activity

2017 ◽  
Vol 175 (3) ◽  
pp. 1269-1282 ◽  
Author(s):  
Vladimir Totev Valkov ◽  
Alessandra Rogato ◽  
Ludovico Martins Alves ◽  
Stefano Sol ◽  
Mélanie Noguero ◽  
...  
Keyword(s):  
Nitrate Transporter ◽  
Transporter Family ◽  
Family Protein

Projection Structure of DtpD (YbgH), a Prokaryotic Member of the Peptide Transporter Family

2009 ◽  
Vol 394 (4) ◽  
pp. 708-717 ◽  
Author(s):  
Fabio Casagrande ◽  
Daniel Harder ◽  
Andreas Schenk ◽  
Marcel Meury ◽  
Zohre Ucurum ◽  
...  
Keyword(s):  
Peptide Transporter ◽  
Transporter Family

scholarly journals Soybean Yellow Stripe-like7 is a symbiosome membrane peptide transporter essential for nitrogen fixation

2020 ◽  
Author(s):  
A Gavrin ◽  
PC Loughlin ◽  
EM Brear ◽  
OW Griffith ◽  
F Bedon ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Nitrogenase Activity ◽  
Metal Transport ◽  
Peptide Transporter ◽  
Excess Iron ◽  
Symbiosome Membrane ◽  
Transporter Family ◽  
Membrane Peptide ◽  
Mature Nodule ◽  
Yellow Stripe

ABSTRACTLegumes form a symbiosis with rhizobia that convert atmospheric nitrogen (N2) to ammonia which they provide to the plant in return for a carbon and nutrient supply. Nodules, developed as part of the symbiosis, harbor rhizobia which are enclosed in the plant-derived symbiosome membrane (SM), to form a symbiosome. In the mature nodule all exchanges between the symbionts occur across the SM. Here we characterize GmYSL7, a member of Yellow stripe-like family which is localized to the SM in soybean nodules. It is expressed specifically in nodule infected cells with expression peaking soon after nitrogenase becomes active. Although most members of the family transport metal complexed with phytosiderophores, GmYSL7 does not. It transports oligopeptides of between four and 12 amino acids. Silencing of GmYSL7 reduces nitrogenase activity and blocks development when symbiosomes contain a single bacteroid. RNAseq of nodules in which GmYSL7 is silenced suggests that the plant initiates a defense response against the rhizobia. There is some evidence that metal transport in the nodules is dysregulated, with upregulation of genes encoding ferritin and vacuolar iron transporter family and downregulation of a gene encoding nicotianamine synthase. However, it is not clear whether the changes are a result of the reduction of nitrogen fixation and the requirement to store excess iron or an indication of a role of GmYSL7 in regulation of metal transport in the nodules. Further work to identify the physiological substrate for GmYSL7 will allow clarification of this role.One sentence summaryGmYSL7 is a symbiosome membrane peptide transporter that is essential for symbiotic nitrogen fixation that when silenced blocks symbiosome development.

scholarly journals TheArabidopsisNRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism

2020 ◽  
Vol 117 (49) ◽  
pp. 31500-31509
Author(s):  
Shunsuke Watanabe ◽  
Naoki Takahashi ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Yuki Aoi ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Peptide Transporter ◽  
Root Tip ◽  
Loss Of Function ◽  
Primary Roots ◽  
Transporter Family ◽  
Family Protein ◽  
Protein Functions ◽  
Root Gravitropism ◽  
Root Tissues

Active membrane transport of plant hormones and their related compounds is an essential process that determines the distribution of the compounds within plant tissues and, hence, regulates various physiological events. Here, we report that theArabidopsisNITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY 7.3 (NPF7.3) protein functions as a transporter of indole-3-butyric acid (IBA), a precursor of the major endogenous auxin indole-3-acetic acid (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-functionnpf7.3mutants showed defective root gravitropism with reduced IBA levels and auxin responses. Nevertheless, the phenotype was restored by exogenous application of IAA but not by IBA treatment.NPF7.3was expressed in pericycle cells and the root tip region including root cap cells of primary roots where the IBA-to-IAA conversion occurs. Our findings indicate that NPF7.3-mediated IBA uptake into specific cells is required for the generation of appropriate auxin gradients within root tissues.

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