scholarly journals A Genome-Wide Association Study of Highly Heritable Agronomic Traits in Durum Wheat

2019 ◽  
Vol 10 ◽  
Author(s):  
Shubin Wang ◽  
Steven Xu ◽  
Shiaoman Chao ◽  
Qun Sun ◽  
Shuwei Liu ◽  
...  
2021 ◽  
Vol 25 (02) ◽  
pp. 388-396
Author(s):  
Yan Li

Uncovering the genetic basis of rice landraces has important applications in breeding. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of the agronomic traits of 60 rice species in Southeast Guizhou. We obtained a total of 178,287,776 reads, 314,065 SLAFs, and 571,521 single nucleotide polymorphisms (SNPs). A neighbor-joining phylogenetic tree, admixture proportions, and principal component analyses revealed that the investigated landraces were divided into japonica (heterozygosity rate 0.062) and indica (heterozygosity rate 0.073) groups. The groupings were consistent with the local classifications of ―He‖ and ―Gu‖ based on the resistance to seed shattering, and the SNPs clustered in the qSH1 gene. The GWAS of eight agronomic traits revealed that the signal peaks at four locations were closely related to previously reported genes or gene regions. This study demonstrates that the SLAF sequencing method combined with a GWAS may be effective for investigating the evolution of rice and identifying genes regulating complex traits in rice landraces cultivated in relatively isolated regions. © 2021 Friends Science Publishers


PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171105 ◽  
Author(s):  
Rodrigo Iván Contreras-Soto ◽  
Freddy Mora ◽  
Marco Antônio Rott de Oliveira ◽  
Wilson Higashi ◽  
Carlos Alberto Scapim ◽  
...  

2020 ◽  
Author(s):  
Dilan S. R. Patiranage ◽  
Elodie Rey ◽  
Nazgol Emrani ◽  
Gordon Wellman ◽  
Karl Schmid ◽  
...  

AbstractQuinoa germplasm preserves useful and substantial genetic variation, yet it remains untapped due to a lack of implementation of modern breeding tools. We have integrated field and sequence data to characterize a large diversity panel of quinoa. Whole-genome sequencing of 310 accessions revealed 2.9 million polymorphic high confidence SNP loci. Highland and Lowland quinoa were clustered into two main groups, with FST divergence of 0.36 and fast LD decay of 6.5 and 49.8 Kb, respectively. A genome-wide association study uncovered 600 SNPs stably associated with 17 agronomic traits. Two candidate genes are associated with thousand seed weight, and a resistance gene analog is associated with downy mildew resistance. We also identified pleiotropically acting loci for four agronomic traits that are highly responding to photoperiod hence important for the adaptation to different environments. This work demonstrates the use of re-sequencing data of an orphan crop, which is partially domesticated to rapidly identify marker-trait association and provides the underpinning elements for genomics-enabled quinoa breeding.


PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255761
Author(s):  
Waltram Ravelombola ◽  
Jun Qin ◽  
Ainong Shi ◽  
Qijian Song ◽  
Jin Yuan ◽  
...  

Soybean [Glycine max (L.) Merr.] is a crop of great interest worldwide. Exploring molecular approaches to increase yield genetic gain has been one of the main challenges for soybean breeders and geneticists. Agronomic traits such as maturity, plant height, and seed weight have been found to contribute to yield. In this study, a total of 250 soybean accessions were genotyped with 10,259 high-quality SNPs postulated from genotyping by sequencing (GBS) and evaluated for grain yield, maturity, plant height, and seed weight over three years. A genome-wide association study (GWAS) was performed using a Bayesian Information and Linkage Disequilibrium Iteratively Nested Keyway (BLINK) model. Genomic selection (GS) was evaluated using a ridge regression best linear unbiased predictor (rrBLUP) model. The results revealed that 20, 31, 37, and 23 SNPs were significantly associated with maturity, plant height, seed weight, and yield, respectively; Many SNPs were mapped to previously described maturity and plant height loci (E2, E4, and Dt1) and a new plant height locus was mapped to chromosome 20. Candidate genes were found in the vicinity of the two SNPs with the highest significant levels associated with yield, maturity, plant height, seed weight, respectively. A 11.5-Mb region of chromosome 10 was associated with both yield and seed weight. Overall, the accuracy of GS was dependent on the trait, year, and population structure, and high accuracy indicates that these agronomic traits can be selected in molecular breeding through GS. The SNP markers identified in this study can be used to improve yield and agronomic traits through the marker-assisted selection and GS in breeding programs.


2017 ◽  
Vol 91 (5) ◽  
pp. 861-873 ◽  
Author(s):  
Yaxi Liu ◽  
Yu Lin ◽  
Shang Gao ◽  
Zhanyi Li ◽  
Jian Ma ◽  
...  

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