scholarly journals Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

2019 ◽  
Vol 10 ◽  
Author(s):  
Wei Yan ◽  
Xiaoyun Wu ◽  
Yanan Li ◽  
Guanghua Liu ◽  
Zhanfei Cui ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Source To Sink

In transformed tobacco cells the apoplasmic invertase inhibitor operates as a regulatory switch of cell wall invertase

1998 ◽  
Vol 13 (2) ◽  
pp. 275-280 ◽  
Author(s):  
Silke Krausgrill ◽  
Steffen Greiner ◽  
Ulrike Köster ◽  
Rolf Vogel ◽  
Thomas Rausch
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Tobacco Cells ◽  
Invertase Inhibitor ◽  
Transformed Tobacco

scholarly journals Induction of a Pea Cell-Wall Invertase Gene by Wounding and Its Localized Expression in Phloem

1996 ◽  
Vol 112 (3) ◽  
pp. 1111-1117 ◽  
Author(s):  
L. Zhang ◽  
N. S. Cohn ◽  
J. P. Mitchell
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Invertase Gene

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

cDNA cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis

Biologia ◽  
2015 ◽  
Vol 70 (8) ◽  
Author(s):  
Chen Liu ◽  
Zhongrui Xu ◽  
Shenwen Cai ◽  
Luan Zhang ◽  
Zhiting Xiong
Keyword(s):  
Cell Wall ◽  
Evolutionary Relationship ◽  
Methylotrophic Yeast ◽  
Cell Wall Invertase ◽  
Reading Frame ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Acid Protein ◽  
Elsholtzia Haichowensis ◽  
A Cell ◽  
Theoretical Molecular Mass

AbstractThe main objective of the present study was to clone, heterologously express and characterize a novel cell wall invertase (FCWI) from a Cu tolerant population of Elsholtzia haichowensis. The full-length FCWI cDNA contained an open reading frame (ORF) of 1671 bp which encoded a 556-amino-acid protein. The theoretical molecular mass and pI of the deduced protein were 62.5 kDa and 9.29, respectively. Phylogenetic analysis showed that FCWI had a closer evolutionary relationship to cell wall invertase of dicot. FCWI was expressed in methylotrophic yeast Pichia pastoris and purified to near homogeneity. Recombinant FCWI enzyme had pH optima of 4.0 and temperature optima of 50◦C. Activity analyses in the presence of various metal cations indicated that FCWI was completely inhibited by Hg

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