scholarly journals Proteomic Analysis of Arabidopsis pldα1 Mutants Revealed an Important Role of Phospholipase D Alpha 1 in Chloroplast Biogenesis

2019 ◽  
Vol 10 ◽  
Author(s):  
Tomáš Takáč ◽  
Tibor Pechan ◽  
Olga Šamajová ◽  
Jozef Šamaj
Keyword(s):  
Phospholipase D ◽  
Proteomic Analysis ◽  
Chloroplast Biogenesis

scholarly journals Specificity of Loxosceles α Clade Phospholipase D Enzymes for Choline-Containing Lipids: Role of a Conserved Aromatic Cage

2021 ◽  
Vol 120 (3) ◽  
pp. 234a
Author(s):  
Emmanuel E. Moutoussamy ◽  
Qaiser Waheed ◽  
Greta Binford ◽  
Matthew Cordes ◽  
Hanif Muhammad Khan ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Aromatic Cage

Role of Phospholipase D Activation in Nervous System Physiology and Pathophysiology

2002 ◽  
Vol 65 (4) ◽  
pp. 1445-1455 ◽  
Author(s):  
Jochen Klein ◽  
Vered Chalifa ◽  
Mordechai Liscovitch ◽  
Konrad Löffelholz
Keyword(s):  
Nervous System ◽  
Phospholipase D ◽  
System Physiology

scholarly journals Corrigendum: Physiological and Proteomic Analysis of the Rice Mutant cpm2 Suggests a Negative Regulatory Role of Jasmonic Acid in Drought Tolerance

2018 ◽  
Vol 9 ◽  
Author(s):  
Rohit Dhakarey ◽  
Manish L. Raorane ◽  
Achim Treumann ◽  
Preshobha K. Peethambaran ◽  
Rachel R. Schendel ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Jasmonic Acid ◽  
Proteomic Analysis ◽  
Regulatory Role ◽  
Rice Mutant ◽  
Negative Regulatory Role

The role of phospholipase D in Glut-4 translocation

2003 ◽  
Vol 19 (6) ◽  
pp. 456-463 ◽  
Author(s):  
Ping Huang ◽  
Michael A. Frohman
Keyword(s):  
Phospholipase D ◽  
Glut 4 ◽  
Glut 4 Translocation

scholarly journals Targeting of Protein Kinase C-ϵ during Fcγ Receptor-dependent Phagocytosis Requires the ϵC1B Domain and Phospholipase C-γ1

2006 ◽  
Vol 17 (2) ◽  
pp. 799-813 ◽  
Author(s):  
Keylon L. Cheeseman ◽  
Takehiko Ueyama ◽  
Tanya M. Michaud ◽  
Kaori Kashiwagi ◽  
Demin Wang ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phospholipase C ◽  
Phospholipase D ◽  
Fluorescent Protein ◽  
Wild Type ◽  
Fcγ Receptor ◽  
Kinase C ◽  
Green Fluorescent

Protein kinase C-ϵ (PKC-ϵ) translocates to phagosomes and promotes uptake of IgG-opsonized targets. To identify the regions responsible for this concentration, green fluorescent protein (GFP)-protein kinase C-ϵ mutants were tracked during phagocytosis and in response to exogenous lipids. Deletion of the diacylglycerol (DAG)-binding ϵC1 and ϵC1B domains, or the ϵC1B point mutant ϵC259G, decreased accumulation at phagosomes and membrane translocation in response to exogenous DAG. Quantitation of GFP revealed that ϵC259G, ϵC1, and ϵC1B accumulation at phagosomes was significantly less than that of intact PKC-ϵ. Also, the DAG antagonist 1-hexadecyl-2-acetyl glycerol (EI-150) blocked PKC-ϵ translocation. Thus, DAG binding to ϵC1B is necessary for PKC-ϵ translocation. The role of phospholipase D (PLD), phosphatidylinositol-specific phospholipase C (PI-PLC)-γ1, and PI-PLC-γ2 in PKC-ϵ accumulation was assessed. Although GFP-PLD2 localized to phagosomes and enhanced phagocytosis, PLD inhibition did not alter target ingestion or PKC-ϵ localization. In contrast, the PI-PLC inhibitor U73122 decreased both phagocytosis and PKC-ϵ accumulation. Although expression of PI-PLC-γ2 is higher than that of PI-PLC-γ1, PI-PLC-γ1 but not PI-PLC-γ2 consistently concentrated at phagosomes. Macrophages from PI-PLC-γ2-/-mice were similar to wild-type macrophages in their rate and extent of phagocytosis, their accumulation of PKC-ϵ at the phagosome, and their sensitivity to U73122. This implicates PI-PLC-γ1 as the enzyme that supports PKC-ϵ localization and phagocytosis. That PI-PLC-γ1 was transiently tyrosine phosphorylated in nascent phagosomes is consistent with this conclusion. Together, these results support a model in which PI-PLC-γ1 provides DAG that binds to ϵC1B, facilitating PKC-ϵ localization to phagosomes for efficient IgG-mediated phagocytosis.

scholarly journals A proteomic analysis of the role of NOS‐like proteins in bacteria

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Andrea B.L. Dupont ◽  
J. Guy Guillemette
Keyword(s):  
Proteomic Analysis

scholarly journals Role of Temperature Stress on Chloroplast Biogenesis and Protein Import in Pea

2009 ◽  
Vol 150 (2) ◽  
pp. 1050-1061 ◽  
Author(s):  
Siddhartha Dutta ◽  
Sasmita Mohanty ◽  
Baishnab C. Tripathy
Keyword(s):  
Temperature Stress ◽  
Protein Import ◽  
Chloroplast Biogenesis
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