scholarly journals Effect of Thermal Stress on Tissue Ultrastructure and Metabolite Profiles During Initiation of Radiata Pine Somatic Embryogenesis

2019 ◽  
Vol 9 ◽  
Author(s):  
Ander Castander-Olarieta ◽  
Itziar A. Montalbán ◽  
Eliana De Medeiros Oliveira ◽  
Emilia Dell’Aversana ◽  
Luisa D’Amelia ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Thermal Stress ◽  
Radiata Pine ◽  
Metabolite Profiles ◽  
Tissue Ultrastructure

Temporal variations in metabolite profiles at different growth phases during somatic embryogenesis of Silybum marianum L.

2014 ◽  
Vol 120 (1) ◽  
pp. 127-139 ◽  
Author(s):  
Mubarak Ali Khan ◽  
Bilal Haider Abbasi ◽  
Huma Ali ◽  
Mohammad Ali ◽  
Mohammad Adil ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Temporal Variations ◽  
Growth Phases ◽  
Silybum Marianum ◽  
Metabolite Profiles

scholarly journals Induction of Radiata Pine Somatic Embryogenesis at High Temperatures Provokes a Long-Term Decrease in DNA Methylation/Hydroxymethylation and Differential Expression of Stress-Related Genes

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1762
Author(s):  
Ander Castander-Olarieta ◽  
Cátia Pereira ◽  
Ester Sales ◽  
Mónica Meijón ◽  
Isabel Arrillaga ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
High Temperatures ◽  
Stress Responses ◽  
Radiata Pine ◽  
Global Methylation ◽  
Protein Coding ◽  
Somatic Plants ◽  
Stress Related Genes ◽  
As Stress ◽  
Stress Mediators

Based on the hypothesis that embryo development is a crucial stage for the formation of stable epigenetic marks that could modulate the behaviour of the resulting plants, in this study, radiata pine somatic embryogenesis was induced at high temperatures (23 °C, eight weeks, control; 40 °C, 4 h; 60 °C, 5 min) and the global methylation and hydroxymethylation levels of emerging embryonal masses and somatic plants were analysed using LC-ESI-MS/ MS-MRM. In this context, the expression pattern of six genes previously described as stress-mediators was studied throughout the embryogenic process until plant level to assess whether the observed epigenetic changes could have provoked a sustained alteration of the transcriptome. Results indicated that the highest temperatures led to hypomethylation of both embryonal masses and somatic plants. Moreover, we detected for the first time in a pine species the presence of 5-hydroxymethylcytosine, and revealed its tissue specificity and potential involvement in heat-stress responses. Additionally, a heat shock protein-coding gene showed a down-regulation tendency along the process, with a special emphasis given to embryonal masses at first subculture and ex vitro somatic plants. Likewise, the transcripts of several proteins related with translation, oxidative stress response, and drought resilience were differentially expressed.

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

2011 ◽  
Vol 5 (2) ◽  
pp. 177-186 ◽  
Author(s):  
I. A. Montalbán ◽  
N. De Diego ◽  
E. Aguirre Igartua ◽  
A. Setién ◽  
P. Moncaleán
Keyword(s):  
Somatic Embryogenesis ◽  
Radiata Pine

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum
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