scholarly journals Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

2019 ◽  
Vol 9 ◽  
Author(s):  
Weijuan Liu ◽  
Yang Xiang ◽  
Xiaoyun Zhang ◽  
Gaoqiang Han ◽  
Xiujuan Sun ◽  
...  
2020 ◽  
Vol 21 (1) ◽  
pp. 351
Author(s):  
Liru Cao ◽  
Pengyu Zhang ◽  
Xiaomin Lu ◽  
Guorui Wang ◽  
Zhenhua Wang ◽  
...  

OSCAs are hyperosmolality-gated calcium-permeable channel proteins. In this study, two co-expression modules, which are strongly associated with maize proline content, were screened by weighted correlation network analysis, including three ZmOSCA family members. Phylogenetic and protein domain analyses revealed that 12 ZmOSCA members were classified into four classes, which all contained DUF221 domain. The promoter region contained multiple core elements responsive to abiotic stresses and hormones. Colinear analysis revealed that ZmOSCAs had diversified prior to maize divergence. Most ZmOSCAs responded positively to ABA, PEG, and NaCl treatments. ZmOSCA2.3 and ZmOSCA2.4 were up-regulated by more than 200-fold under the three stresses, and showed significant positive correlations with proline content. Yeast two-hybrid and bimolecular fluorescence complementation indicated that ZmOSCA2.3 and ZmOSCA2.4 proteins interacted with ZmEREB198. Over-expression of ZmOSCA2.4 in Arabidopsis remarkably improved drought resistance. Moreover, over-expression of ZmOSCA2.4 enhanced the expression of drought tolerance-associated genes and reduced the expression of senescence-associated genes. We also found that perhaps ZmOSCA2.4 was regulated by miR5054.The results provide a high-quality molecular resource for selecting resistant breeding, and lay a foundation for elucidating regulatory mechanism of ZmOSCA under abiotic stresses.


2019 ◽  
Vol 38 (9) ◽  
pp. 1109-1125 ◽  
Author(s):  
Jin Seok Yoon ◽  
Jae Yoon Kim ◽  
Man Bo Lee ◽  
Yong Weon Seo

Gene ◽  
2010 ◽  
Vol 452 (2) ◽  
pp. 54-62 ◽  
Author(s):  
Osmany Chacón ◽  
Marleny González ◽  
Yunior López ◽  
Roxana Portieles ◽  
Merardo Pujol ◽  
...  

2000 ◽  
Vol 23 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Yusuke Saijo ◽  
Shingo Hata ◽  
Junko Kyozuka ◽  
Ko Shimamoto ◽  
Katsura Izui

BMB Reports ◽  
2014 ◽  
Vol 47 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Joungsu Joo ◽  
Hae Jong Choi ◽  
Youn Hab Lee ◽  
Sarah Lee ◽  
Choong Hwan Lee ◽  
...  

2008 ◽  
Vol 30 (12) ◽  
pp. 2191-2198 ◽  
Author(s):  
Jian-Qiang Chen ◽  
Xiu-Ping Meng ◽  
Yun Zhang ◽  
Mian Xia ◽  
Xi-Ping Wang

2010 ◽  
Vol 33 (2) ◽  
pp. 403-409 ◽  
Author(s):  
Xiaohui Zhang ◽  
Zhe Zou ◽  
Pengjuan Gong ◽  
Junhong Zhang ◽  
Khurram Ziaf ◽  
...  

2018 ◽  
Vol 283 ◽  
pp. 11-21 ◽  
Author(s):  
Diego A. Peralta ◽  
Alejandro Araya ◽  
Diego F. Gomez-Casati ◽  
María V. Busi

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