scholarly journals BrLAS, a GRAS Transcription Factor From Brassica rapa, Is Involved in Drought Stress Tolerance in Transgenic Arabidopsis

2018 ◽  
Vol 9 ◽  
Author(s):  
Pan Li ◽  
Bin Zhang ◽  
Tongbing Su ◽  
Peirong Li ◽  
Xiaoyun Xin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Brassica Rapa ◽  
Transgenic Arabidopsis ◽  
Drought Stress Tolerance ◽  
Gras Transcription Factor

Expression of maize heat shock transcription factor gene ZmHsf06 enhances the thermotolerance and drought-stress tolerance of transgenic Arabidopsis

2015 ◽  
Vol 42 (11) ◽  
pp. 1080 ◽  
Author(s):  
Hui-cong Li ◽  
Hua-ning Zhang ◽  
Guo-liang Li ◽  
Zi-hui Liu ◽  
Yan-min Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Heat Shock ◽  
Stress Tolerance ◽  
Transgenic Arabidopsis ◽  
Germination Rate ◽  
Heat Shock Transcription Factor ◽  
Drought Stress Tolerance ◽  
Acquired Thermotolerance ◽  
A Genome

Based on the information of 25 heat shock transcription factor (Hsf) homologues in maize according to a genome-wide analysis, ZmHsf06 was cloned from maize leaves and transformed into Arabidopsis thaliana (L. Heynh.) (ecotype, Col-0). Three transgenic positive lines were selected to assess the basic and acquired thermotolerance and drought-stress tolerance under stresses and for some physiological assays. The sequence analysis indicates that ZmHsf06 contained the characteristic domains of class A type plant Hsfs. The results of qRT–PCR showed that the expression levels of ZmHsf06 were elevated by heat shock and drought stress to different extents in three transgenic lines. Phenotypic observation shows that compared with the Wt (wild-type) controls, the overexpressing ZmHsf06 of Arabidopsis plants have enhanced basal and acquired thermotolerance, stronger drought-stress tolerance and growth advantages under mild heat stress conditions. These results are further confirmed by physiological and biochemical evidence that transgenic Arabidopsis plants exhibit higher seed germination rate, longer axial-root length, higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), higher leaf chlorophyll content, but lower relative electrical conductivity (REC), malondialdehyde (MDA) and osmotic potential (OP) than the Wt controls after heat shock and drought treatments. ZmHsf06 may be a central representative of maize Hsfs and could be useful in molecular breeding of maize or other crops for enhanced tolerances, particularly during terminal heat and drought stresses.

scholarly journals Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 142 ◽  
Author(s):  
Mohamed El-Esawi ◽  
Aisha Alayafi
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Gas Exchange ◽  
Stress Tolerance ◽  
Plant Biomass ◽  
Oilseed Crop ◽  
Wild Type ◽  
Ros Scavenging ◽  
Antioxidant Genes ◽  
Drought Stress Tolerance

Drought stress significantly restricts plant growth and crop productivity. Cotton is the most important textile fiber and oilseed crop worldwide, and its cultivation is affected by drought stress, particularly in dry regions. Improving cotton tolerance to drought stress using the advanced genetic engineering technologies is a promising strategy to maintain crop production and fiber quality and meet the increasing worldwide fiber and oil demand. Dehydration-responsive element binding (DREB) transcription factors play a main role in regulating stresses-tolerance pathways in plant. This study investigated whether potato DREB2 (StDREB2) overexpression can improve drought tolerance in cotton. StDREB2 transcription factor was isolated and overexpressed in cotton. Plant biomass, boll number, relative water content, soluble sugars content, soluble protein content, chlorophyll content, proline content, gas-exchange parameters, and antioxidants enzymes (POD, CAT, SOD, GST) activity of the StDREB2-overexpressing cotton plants were higher than those of wild type plants. By contrast, the contents of malondialdehyde, hydrogen peroxide and superoxide anion of StDREB2-overexpressing transgenic plants were significantly lower than that of the wild type plants. Moreover, the transgenic cotton lines revealed higher expression levels of antioxidant genes (SOD, CAT, POD, GST) and stress-tolerant genes (GhERF2, GhNAC3, GhRD22, GhDREB1A, GhDREB1B, GhDREB1C) compared to wild-type plants. Taken together, these findings showed that StDREB2 overexpression augments drought stress tolerance in cotton by inducing plant biomass, gas-exchange characteristics, reactive oxygen species (ROS) scavenging, antioxidant enzymes activities, osmolytes accumulation, and expression of stress-related genes. As a result, StDREB2 could be an important candidate gene for drought-tolerant cotton breeding.

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