scholarly journals Effects of Transgenerational Plasticity on Morphological and Physiological Properties of Stoloniferous Herb Centella asiatica Subjected to High/Low Light

2018 ◽  
Vol 9 ◽  
Author(s):  
Kenian Li ◽  
Jinsong Chen ◽  
Qing Wei ◽  
Qian Li ◽  
Ningfei Lei
2022 ◽  
Author(s):  
Xiao Xiao ◽  
Linxuan He ◽  
Xiaomei Zhang ◽  
Yu Jin ◽  
Jinsong Chen

Abstract Transgenerational plasticity allows offsprings to be more adaptive in the environmental conditions experienced by their parents. It is suggested that differential effects of transgenerational plasticity on growth performance of offspring ramets may help to understand successful invasion of invasive plant with clonal growth comparing with its congeneric native one. A pot experiment using invasive herb Wedelia trilobata and its congeneric native species Wedelia chinensis was conducted to investigate differential effects of high/low light treatment experienced by mother ramets on morphological and photosynthetic properties of offspring ramets subjected to stressful low light treatment. For W. chinensis, stolon length and maximum carboxylation rate (Vmax) in offspring ramets from mother ramets subjected to low light treatment were significantly greater than those in offspring ramets from mother ramets subjected to high light treatment. For W. trilobata, leaf area and potential maximum net photosynthetic rate (Pmax) in offspring ramets from mother ramets subjected to low light treatment were significantly greater than those in offspring ramets from mother ramets subjected to high light treatment. We tentatively concluded that effects of transgenerational plasticity on morphological and photosynthetic properties among clonal plants could be species-specific. In addition, more favorable effect of transgenerational plasticity on growth performance was observed in the invasive plant than in its congeneric native species. It is suggested that transgenerational plasticity may be very important for successful invasion of invasive plant with clonal growth, especially in maternal environmental conditions. So, our experiment provides new insight into invasive mechanism of invasive plants.


Author(s):  
G.Y. Fan ◽  
J.M. Cowley

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.


Author(s):  
W. Lin ◽  
J. Gregorio ◽  
T.J. Holmes ◽  
D. H. Szarowski ◽  
J.N. Turner

A low-light level video microscope with long working distance objective lenses has been built as part of our integrated three-dimensional (3-D) light microscopy workstation (Fig. 1). It allows the observation of living specimens under sufficiently low light illumination that no significant photobleaching or alternation of specimen physiology is produced. The improved image quality, depth discrimination and 3-D reconstruction provides a versatile intermediate resolution system that replaces the commonly used dissection microscope for initial image recording and positioning of microelectrodes for neurobiology. A 3-D image is displayed on-line to guide the execution of complex experiments. An image composed of 40 optical sections requires 7 minutes to process and display a stereo pair.The low-light level video microscope utilizes long working distance objective lenses from Mitutoyo (10X, 0.28NA, 37 mm working distance; 20X, 0.42NA, 20 mm working distance; 50X, 0.42NA, 20 mm working distance). They provide enough working distance to allow the placement of microelectrodes in the specimen.


Author(s):  
Ray Keller

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)


Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
T Kaensaksiri ◽  
P Soontornchainaksaeng ◽  
N Soonthornchareonnon ◽  
S Prathanturarug
Keyword(s):  

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
A Alqahtani ◽  
W Tongkao-on ◽  
KM Li ◽  
V Razmovski-Naumovski ◽  
B Kimble ◽  
...  

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
N Gray ◽  
J Morré ◽  
J Kelley ◽  
C Maier ◽  
F Stevens ◽  
...  

2019 ◽  
Vol 57 (6) ◽  
pp. 665 ◽  
Author(s):  
Yen Thi Hoang ◽  
Quynh Thi Thu Tran ◽  
Ha Hoang Chu ◽  
Tuyen Thi Do ◽  
Thanh Tat Dang ◽  
...  

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.


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