scholarly journals Specific Fluorescence in Situ Hybridization (FISH) Test to Highlight Colonization of Xylem Vessels by Xylella fastidiosa in Naturally Infected Olive Trees (Olea europaea L.)

2018 ◽  
Vol 9 ◽  
Author(s):  
Massimiliano Cardinale ◽  
Andrea Luvisi ◽  
Joana B. Meyer ◽  
Erika Sabella ◽  
Luigi De Bellis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Olea Europaea ◽  
Xylella Fastidiosa ◽  
Specific Fluorescence ◽  
Olea Europaea L ◽  
Olive Trees ◽  
Olea Europaéa

Comparison of detection specificity of nitrifying bacteria in biofilm using fluorescence in situ hybridization and in situ fluorescent antibody methods

2003 ◽  
Vol 47 (5) ◽  
pp. 129-132
Author(s):  
N. Noda ◽  
Y. Ebie ◽  
M. Matsumura ◽  
S. Tsuneda ◽  
A. Hirata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Specific Fluorescence ◽  
Antibody Method ◽  
In Situ Detection ◽  
Detection Specificity ◽  
Fluorescent Antibody Method

The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) methods are very useful in the in situ detection of specific bacteria like nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and in situ fluorescent antibody methods was examined. As a result, no specific fluorescence was observed with either method when FISH was performed followed by the in situ fluorescent antibody method; however, when the in situ fluorescent antibody method was performed first followed by FISH, specific fluorescence was observed in both cases. Moreover, it was suggested that the detection specificities of FISH and the in situ fluorescent antibody method are almost identical.

Early water stress detection on olive trees (Olea europaea L. cvs ‘chemlali’ and ‘Chetoui’) using the leaf patch clamp pressure probe

2016 ◽  
Vol 131 ◽  
pp. 20-28 ◽  
Author(s):  
Feten Aissaoui ◽  
Hechmi Chehab ◽  
Besma Bader ◽  
Angham Ben Salem ◽  
Naouraz M’barki ◽  
...  
Keyword(s):  
Water Stress ◽  
Patch Clamp ◽  
Olea Europaea ◽  
Pressure Probe ◽  
Stress Detection ◽  
Olea Europaea L ◽  
Olive Trees ◽  
Olea Europaéa

Effect of prolonged vegetative reproduction of olive tree cultivars (Olea europaea L.) in mitochondrial homoplasmy and heteroplasmy

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 377-381 ◽  
Author(s):  
Angel García-Díaz ◽  
Ricardo Oya ◽  
Antonio Sánchez ◽  
Francisco Luque
Keyword(s):  
Mitochondrial Genome ◽  
Sexual Reproduction ◽  
Olea Europaea ◽  
Vegetative Reproduction ◽  
Intergenic Spacer Region ◽  
Olea Europaea L ◽  
Chloroplast Genomes ◽  
Mitochondrial Region ◽  
Olive Trees ◽  
Olea Europaéa

The inheritance of mitochondrial and chloroplast genomes does not follow Mendelian laws, but proceeds by vegetative segregation. Most organisms show organelle homoplasmy, which is probably produced and maintained during sexual reproduction. We have tested the effect of prolonged vegetative multiplication in the maintenance of mitochondrial homoplasmy and the generation of heteroplasmy in cultivated olive trees, Olea europaea L. Seven trees, each representing a different variety of olive, were analysed by the study of an intergenic spacer region of the mitochondrial genome. A very high level of heteroplasmy was detected in all cases. We found multiple genome variants of the sequence analysed. The frequency of genomes with no changes in the spacer region was 11.5%. This means that 88.5% of genomes contain at least one change. The same spacer mitochondrial region was sequenced in several clones from four olive trees of a second generation of sexually reproduced trees. In these trees, many clones were identical and had no changes, which represents a clear reduction of the heteroplasmy (p < 0.001). Therefore, this work supports the relevance of the role of sexual reproduction in the maintenance of mitochondrial homoplasmy and also shows that mutations accumulate in a non-coding sequence of the mitochondrial genome when vegetative propagation is maintained for a long period of time.Key words: mitochondrial genome, homoplasmy, heteroplasmy, olive trees, vegetative reproduction, sexual reproduction.

Effects of the planting density on water relations and production of ‘Chemlali’ olive trees (Olea europaea L.)

Trees ◽  
2010 ◽  
Vol 24 (6) ◽  
pp. 1137-1142 ◽  
Author(s):  
Mokhtar Guerfel ◽  
Youssef Ouni ◽  
Dalenda Boujnah ◽  
Mokhtar Zarrouk
Keyword(s):  
Water Relations ◽  
Olea Europaea ◽  
Planting Density ◽  
Olea Europaea L ◽  
Olive Trees ◽  
Olea Europaéa
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