scholarly journals Isolation and Functional Characterization of a Floral Repressor, BcMAF1, From Pak-choi (Brassica rapa ssp. Chinensis)

2018 ◽  
Vol 9 ◽  
Author(s):  
Feiyi Huang ◽  
Tongkun Liu ◽  
Xilin Hou
Keyword(s):  
Brassica Rapa ◽  
Functional Characterization ◽  
Pak Choi ◽  
Floral Repressor

Isolation and functional characterization of a floral repressor, BcFLC2, from Pak-choi (Brassica rapa ssp. chinensis)

Planta ◽  
2018 ◽  
Vol 248 (2) ◽  
pp. 423-435 ◽  
Author(s):  
Feiyi Huang ◽  
Tongkun Liu ◽  
Jin Wang ◽  
Xilin Hou
Keyword(s):  
Brassica Rapa ◽  
Functional Characterization ◽  
Pak Choi ◽  
Floral Repressor

scholarly journals Identification and Functional Characterization of a Cold-Related Protein, BcHHP5, in Pak-Choi (Brassica rapa ssp. chinensis)

2018 ◽  
Vol 20 (1) ◽  
pp. 93
Author(s):  
Jin Wang ◽  
Feiyi Huang ◽  
Xiong You ◽  
Xilin Hou
Keyword(s):  
Brassica Rapa ◽  
Abiotic Stresses ◽  
Quantitative Polymerase Chain Reaction ◽  
Functional Characterization ◽  
Regulation Mechanism ◽  
Pak Choi ◽  
Negative Effect ◽  
Polymerase Chain ◽  
Related Proteins

In plants, heptahelical proteins (HHPs) have been shown to respond to a variety of abiotic stresses, including cold stress. Up to the present, the regulation mechanism of HHP5 under low temperature stress remains unclear. In this study, BcHHP5 was isolated from Pak-choi (Brassica rapa ssp. chinensis cv. Suzhouqing). Sequence analysis and phylogenetic analysis indicated that BcHHP5 in Pak-choi is similar to AtHHP5 in Arabidopsis thaliana. Structure analysis showed that the structure of the BcHHP5 protein is relatively stable and highly conservative. Subcellular localization indicated that BcHHP5 was localized on the cell membrane and nuclear membrane. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that BcHHP5 was induced to express by cold and other abiotic stresses. In Pak-choi, BcHHP5-silenced assay, inhibiting the action of endogenous BcHHP5, indicated that BcHHP5-silenced might have a negative effect on cold tolerance, which was further confirmed. All of these results indicate that BcHHP5 might play a role in abiotic response. This work can serve as a reference for the functional analysis of other cold-related proteins from Pak-choi in the future.

Cloning and functional characterization of a floral repressor gene from Lavandula angustifolia

Planta ◽  
2020 ◽  
Vol 251 (2) ◽  
Author(s):  
Rebecca S. Wells ◽  
Ayelign M. Adal ◽  
Lina Bauer ◽  
Elaheh Najafianashrafi ◽  
Soheil S. Mahmoud
Keyword(s):  
Functional Characterization ◽  
Lavandula Angustifolia ◽  
Repressor Gene ◽  
Floral Repressor

Expression profiling and characterization of cold, freezing-related genes from Brassica rapa cultivars

2016 ◽  
Vol 63 (2) ◽  
pp. 105-115 ◽  
Author(s):  
Senthil Kumar Thamilarasan ◽  
Jong-In Park ◽  
Hee-Jeong Jung ◽  
Mi-Young Chung ◽  
Yong-Gu Cho ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
In Silico ◽  
Functional Characterization ◽  
Freezing Stress ◽  
Biological Database ◽  
Structural Domain ◽  
Freezing Resistance ◽  
Data Set ◽  
Go Annotation

Brassica is a very important vegetable group worldwide. Cold and freezing stress are the major environmental factors that limit the productivity of Brassica. In this study, we retrieved 903 stress-chilled unigenes and the unique transcripts were classified functionally using gene ontology (GO) hierarchy, Kyoto Encyclopedia of Genes and Genomes (KEGG). KEGG orthology and structural domain data were obtained from the biological database to the unigene data set. Unigene data sets provide a wide outlook of functional characterization of Brassica rapa. In silico analysis revealed 94.2% of unigenes to be well annotated toward Reeds one-dimensional concept. On the basis of similarity searches and GO annotation of biological process (BP), 139, 113, and 57 sequences showed a response to stress (SR), abiotic stimulus (AS), and biotic stimulus (BS), respectively. To validate this observation, seven unigenes were randomly selected from AS that are known to be associated with cold stress from previous studies in other species and these genes were examined by real-time polymerase chain reaction to understand the change in expression pattern under cold and freezing stress. In silico study of occurrence in the AS subcategory revealed the presence of 138 single sequence repeats, some of which are being explored to assess genetic diversity among B. rapa. Thus, the unigene data set provides valid resources for discovering the potential genes related to cold and freezing resistance in B. rapa cultivars, and can be useful resources for genetic engineering of Brassica sp.

scholarly journals Functional characterization of C-TERMINALLY ENCODED PEPTIDE (CEP) family in Brassica rapa L

2021 ◽  
Author(s):  
Ziwen Qiu ◽  
Keqing Zhuang ◽  
Yiting Liu ◽  
Xiaomin Ge ◽  
Chen Chen ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Functional Characterization

Characterization of drought and freezing tolerance of three representative cultivars of pak choi (Brassica rapa ssp. chinensis)

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Ying Huang ◽  
Yongbing Ren ◽  
Ren Wang ◽  
Tingting Fan ◽  
Haikun Jiang ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Freezing Tolerance ◽  
Pak Choi

scholarly journals Molecular and functional characterization of a Brmecp gene encoding 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Brassica rapa

2012 ◽  
Vol 39 (3) ◽  
pp. 189-195
Author(s):  
Yu-Jin Jung ◽  
Jang-Sun Choi ◽  
Ju-Nam Sun ◽  
Ill-Sup Nou ◽  
Yong-Gu Cho ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Functional Characterization ◽  
Gene Encoding

scholarly journals Isolation and functional characterization of BrUGT gene encoding a UDP-glycosyltransferase from Chinese cabbage (Brassica rapa)

2012 ◽  
Vol 39 (3) ◽  
pp. 212-218 ◽  
Author(s):  
Yu-Jin Jung ◽  
Hye-Jung Lee ◽  
Jang-Sun Choi ◽  
Yong-Gu Cho ◽  
Ill-Sup Nou ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Functional Characterization ◽  
Gene Encoding
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