scholarly journals Overexpression of SoCYP85A1, a Spinach Cytochrome p450 Gene in Transgenic Tobacco Enhances Root Development and Drought Stress Tolerance

2017 ◽  
Vol 8 ◽  
Author(s):  
Fangmeng Duan ◽  
Jun Ding ◽  
Dongsun Lee ◽  
Xueli Lu ◽  
Yuqi Feng ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Root Development ◽  
Cytochrome P450 Gene ◽  
P450 Gene ◽  
Drought Stress Tolerance

scholarly journals CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 161 ◽  
Author(s):  
Muhammad Junaid Rao ◽  
Yuantao Xu ◽  
Xiaomei Tang ◽  
Yue Huang ◽  
Jihong Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Wild Type ◽  
Ros Scavenging ◽  
Cytochrome P450 Gene ◽  
P450 Gene ◽  
Large Gene ◽  
Transgenic Lines

CYTOCHROME P450s genes are a large gene family in the plant kingdom. Our earlier transcriptome data revealed that a CYTOCHROME P450 gene of Citrus sinensis (CsCYT75B1) was associated with flavonoid metabolism and was highly induced after drought stress. Here, we characterized the function of CsCYT75B1 in drought tolerance by overexpressing it in Arabidopsis thaliana. Our results demonstrated that the overexpression of the CsCYT75B1 gene significantly enhanced the total flavonoid contents with increased antioxidant activity in transgenic Arabidopsis. The gene expression results showed that several genes that are responsible for the biosynthesis of antioxidant flavonoids were induced by 2–12 fold in transgenic Arabidopsis lines. After 14 days of drought stress, all transgenic lines displayed an enhanced tolerance to drought stress along with accumulating antioxidant flavonoids with lower superoxide radicals and reactive oxygen species (ROS) than wild type plants. In addition, drought-stressed transgenic lines possessed higher antioxidant enzymatic activities than wild type transgenic lines. Moreover, the stressed transgenic lines had significantly lower levels of electrolytic leakage than wild type transgenic lines. These results demonstrate that the CsCYT75B1 gene of sweet orange functions in the metabolism of antioxidant flavonoid and contributes to drought tolerance by elevating ROS scavenging activities.

TaASR1, a transcription factor gene in wheat, confers drought stress tolerance in transgenic tobacco

2013 ◽  
Vol 36 (8) ◽  
pp. 1449-1464 ◽  
Author(s):  
WEI HU ◽  
CHAO HUANG ◽  
XIAOMIN DENG ◽  
SHIYI ZHOU ◽  
LIHONG CHEN ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Drought Stress Tolerance
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