scholarly journals Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways

2017 ◽  
Vol 8 ◽  
Author(s):  
Song Song ◽  
Jian Chang ◽  
Chongjun Ma ◽  
Yan-Wen Tan
Keyword(s):  
Signal Transduction ◽  
Single Molecule ◽  
Plant Hormone ◽  
Signal Transduction Pathways ◽  
Single Molecule Fluorescence ◽  
Fluorescence Methods

scholarly journals Single-molecule fluorescence vistas of how lipids regulate membrane proteins

2021 ◽  
Author(s):  
Alyssa E. Ward ◽  
Yujie Ye ◽  
Jennifer A. Schuster ◽  
Shushu Wei ◽  
Francisco N. Barrera
Keyword(s):  
Membrane Proteins ◽  
Physical Properties ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Short Review ◽  
Single Molecule Fluorescence ◽  
Determining Factors ◽  
Fluorescence Methods ◽  
Basic Understanding ◽  
Sample Heterogeneity

The study of membrane proteins is undergoing a golden era, and we are gaining unprecedented knowledge on how this key group of proteins works. However, we still have only a basic understanding of how the chemical composition and the physical properties of lipid bilayers control the activity of membrane proteins. Single-molecule (SM) fluorescence methods can resolve sample heterogeneity, allowing to discriminate between the different molecular populations that biological systems often adopt. This short review highlights relevant examples of how SM fluorescence methodologies can illuminate the different ways in which lipids regulate the activity of membrane proteins. These studies are not limited to lipid molecules acting as ligands, but also consider how the physical properties of the bilayer can be determining factors on how membrane proteins function.

Surfing on a new wave of single-molecule fluorescence methods

2010 ◽  
Vol 7 (3) ◽  
pp. 031001 ◽  
Author(s):  
Johannes Hohlbein ◽  
Kristofer Gryte ◽  
Mike Heilemann ◽  
Achillefs N Kapanidis
Keyword(s):  
Single Molecule ◽  
New Wave ◽  
Single Molecule Fluorescence ◽  
Fluorescence Methods

scholarly journals RNA-Seq And WGCNA Analysis Identifies Salt-Responsive Genes In Pear (Pyrus Ussuriensis Maxim)

2021 ◽  
Author(s):  
Qiming Chen ◽  
Huizhen Dong ◽  
Zhihua Xie ◽  
Kaijie Qi ◽  
Xiaosan Huang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Hormone ◽  
Enrichment Analysis ◽  
Salt Resistance ◽  
Mapk Signaling ◽  
Differentially Expressed ◽  
Signal Transduction Pathways ◽  
Rna Seq

Abstract Background: Pear is one of the most abundant fruit crops and has been cultivated world-wide. However, the salt injury events caused by increased salinity limited the distribution and sustainable production of pear crops. Therefore, it is needed to take further efforts to understand the genetics and mechanisms of salt tolerance to improved salt resistance and productivity.Results: In this work, we analyzed the dynamic transcriptome of pear (Pyrus ussuriensis Maxim) under salt stress by using RNA-Seq and WGCNA. A total of 3540, 3831, 8374, 6267 and 5381 genes were identified that were differentially expressed after exposure to 200mM NaCl for 4, 6, 12, 24 and 48 hours, respectively, and 1163 genes were shared among the five comparisons. KEGG enrichment analysis of these DEGs (differentially expressed genes) revealed that “MAPK signaling” and “Plant hormone signal transduction” pathways were highly enriched. Meanwhile, 622 DEGs identified from WGCNA were highly correlated with these pathways, and some of them were able to indicate the salt tolerance of pear varieties. In addition, we provide a network to demonstrate the time-sequence of these co-expressed MAPK and hormone related genes.Conclusion: A comprehensive analysis about salt-responsive pear transcriptome were performed by using RNA-Seq and WGCNA. We demonstrated that “MAPK signaling” and “Plant hormone signal transduction” pathways were highly recruited during salt stress, and provided new insights into the metabolism of plant hormones related signaling at transcriptome level underlying salt resistance in pear. The dynamic transcriptome data obtained from this study and these salt-sensitive DEGs may provide potential genes as suitable targets for further biotechnological manipulation to improve pear salt tolerance.

scholarly journals Transcriptome analysis of the impact of exogenous methyl jasmonate on the opening of sorghum florets

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248962
Author(s):  
Suifei Liu ◽  
Yongqi Fu ◽  
Yongming He ◽  
Xiaochun Zeng
Keyword(s):  
Signal Transduction ◽  
Methyl Jasmonate ◽  
Molecular Mechanism ◽  
Linolenic Acid ◽  
Transcriptome Analysis ◽  
Metabolic Pathway ◽  
Plant Hormone ◽  
Plasma Cells ◽  
Sucrose Metabolism ◽  
Signal Transduction Pathways

Background Methyl Jasmonate (MeJA) could promote the opening of sorghum florets, but the molecular mechanism remains unclear. Objective We aimed to investigate the molecular mechanism of exogenous MeJA in promoting the opening of sorghum florets. Methods Hybrid sorghum Aikang-8 was selected as the test material in this study. Sorghum plants of uniform growth with approximately 20%-25% florets open were selected and treated with 0, 0.5 and 2.0 mmol/L of MeJA. Totally there were 27 samples with lodicules removed were obtained at different time points and used for the transcriptome analysis using the BGISEQ_500RS platform. Results The results showed the sorghum florets opened earlier than the control after the treatment with exogenous MeJA, and the promotive effect increased along with the increase of exogenous MeJA concentration. The number of differentially expressed genes (DEGs) in plasma cells increased with the increase of MeJA concentration, whether up- or down-regulated, after the exogenous MeJA treatment. Besides, the number of metabolic pathways was also positively correlated with the concentration of MeJA. GO and KEGG analysis suggested the DEGs were mainly enriched in starch and sucrose metabolism-related pathways (i.e., LOC8063704, LOC8083539 and LOC8056206), plant hormone signal transduction pathways (i.e., LOC8084842, LOC8072010, and LOC8057408), energy metabolic pathway (i.e., LOC8076139) and the α-linolenic acid metabolic pathway (i.e., LOC8055636, LOC8057399, LOC8063048 and LOC110430730). Functional analysis of target genes showed that two genes named LOC-1 (LOC8063704) and LOC-2 (LOC8076139) could induce the earlier flowering of Arabidopsis thaliana. Conclusion The results of this study suggest that exogenous MeJA treatments could induce the up- or down- regulation of genes related to starch and sucrose metabolism, -linolenic acid metabolism and plant hormone signal transduction pathways in the plasma cells of sorghum florets, thereby promoting the opening of sorghum florets.

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