scholarly journals Probing the Single Key Amino Acid Responsible for the Novel Catalytic Function of ent-Kaurene Oxidase Supported by NADPH-Cytochrome P450 Reductases in Tripterygium wilfordii

2017 ◽  
Vol 8 ◽  
Author(s):  
Ping Su ◽  
Hongyu Guan ◽  
Yifeng Zhang ◽  
Xing Wang ◽  
Linhui Gao ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Amino Acid ◽  
The Novel ◽  
Tripterygium Wilfordii ◽  
Nadph Cytochrome ◽  
Catalytic Function

scholarly journals Tobacco Cutworm (Spodoptera Litura) Larvae Silenced in the NADPH-Cytochrome P450 Reductase Gene Show Increased Susceptibility to Phoxim

2019 ◽  
Vol 20 (15) ◽  
pp. 3839 ◽  
Author(s):  
Hong-Yi Ji ◽  
Christian Staehelin ◽  
Yan-Ping Jiang ◽  
Shi-Wei Liu ◽  
Zhi-Hui Ma ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Amino Acid ◽  
Spodoptera Litura ◽  
Fat Body ◽  
Larval Mortality ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Amino Acid Sequences ◽  
Cytochrome P450 Monooxygenases ◽  
Expression Levels ◽  
Nadph Cytochrome

Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductases (CPRs) function as redox partners of cytochrome P450 monooxygenases (P450s). CPRs and P450s in insects have been found to participate in insecticide resistance. However, the CPR of the moth Spodoptera litura has not been well characterized yet. Based on previously obtained transcriptome information, a full-length CPR cDNA of S. litura (SlCPR) was PCR-cloned. The deduced amino acid sequence contains domains and residues predicted to be essential for CPR function. Phylogenetic analysis with insect CPR amino acid sequences showed that SlCPR is closely related to CPRs of Lepidoptera. Quantitative reverse transcriptase PCR (RT-qPCR) was used to determine expression levels of SlCPR in different developmental stages and tissues of S. litura. SlCPR expression was strongest at the sixth-instar larvae stage and fifth-instar larvae showed highest expression in the midgut. Expression of SlCPR in the midgut and fat body was strongly upregulated when fifth-instar larvae were exposed to phoxim at LC15 (4 μg/mL) and LC50 (20 μg/mL) doses. RNA interference (RNAi) mediated silencing of SlCPR increased larval mortality by 34.6% (LC15 dose) and 53.5% (LC50 dose). Our results provide key information on the SlCPR gene and indicate that SlCPR expression levels in S. litura larvae influence their susceptibility to phoxim and possibly other insecticides.

The roles of amino acid residues at positions 216 and 219 in the structural stability and metabolic functions of rat cytochrome P450 2D1 and 2D2

2008 ◽  
Vol 172 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Shizuo Narimatsu ◽  
Kimio Kiryu ◽  
Rei Yonemoto ◽  
Manabu Yoshino ◽  
Mitsuko Kobatake ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Amino Acid ◽  
Structural Stability ◽  
Amino Acid Residues ◽  
Metabolic Functions
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